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Effects of neutral phytase on growth performance and phosphorus utilization in crucian carp(Carassius auratus)

Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2017年第10期18卷 886-896页

作者： xin-zheng nie Sha CHEN Xiao-xu ZHANG Bin-yang DAI Li-chun QIAN Key Laboratory of Nutrition and Feed Science in East China Ministry of AgricultureCollege of Animal Sciences Zhejiang Universit

A feeding trial was conducted for nine weeks to investigate the effects of partially replacing Ca（H2PO4）2 with neutral phytase on the growth performance, phosphorus utilization, nutrient digestibility, serum biochemical parameters, bone and carcass mineral composition, and digestive-enzyme-specific activity in crucian carp（Carassius auratus）.The diets prepared with 0.8%, 0%, and 1.8% Ca（H2PO4）2（1%=1 g/100 g） supplements were regarded as the P1E0, negative control（NC）, and positive control（PC） groups, respectively; the other three experimental diets were prepared with the addition of 200, 300, and 500 U/kg of neutral phytase, respectively, based on the P1E0 *** hundred and eighty-four fish（（1.50±0.01） g） were randomly distributed in the six treatments with four replicates *** fish were initially fed with 2%–3% diets of their body weight per day, with feeding twice daily（08：00 and 16：00）, under a 12-h light/12-h dark cycle at the temperature of（27.56±0.89） ℃.The results showed that supplemental phytase at different levels in the diet improved the final body weight, average daily gain, feed conversion ratio, phosphorus utilization, and protein efficiency ratio of crucian carp（P〈0.05）.Phytase supplementation increased the mineral content in serum（P）, bone（P, Ca）, and carcass（P, Ca, Zn, Na, and Mg）（P〈0.05）; the trypsin and chymotrypsin activity soared when fed with the phytase-supplemented diets（P〈0.05）.We may conclude that supplemental dietary neutral phytase improved the growth performance, phosphorus utilization as well as nutrient utilization in crucian carp, and it can be considered an important nutritional replacement for Ca（H2PO4）2.

关键词： Neutral phytase, Crucian carp (Carassius auratus) Growth performance Phosphorus utilization Apparent digestibility coefficient Body composition

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Characterization of β-glucosidase from Aspergillus terreus and its application in the hydrolysis of soybean isoflavones

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2016年第6期17卷 455-464页

作者： Feng-ying YAN Wei XIA Xiao-xu ZHANG Sha CHEN xin-zheng nie Li-chun QIAN The Key Laboratory of Molecular Animal Nutrition Ministry of Education College of Animal Sciences Zhejiang University

An extracellular β-glucosidase produced by Aspergillus terreus was identified, purified, characterized and was tested for the hydrolysis of soybean isofiavone. Matrix-assisted laser desorption/ionization with tandem time-of- flight/time-of-flight mass spectrometry （MALDI-TOF/TOF MS） revealed the protein to be a member of the glycosyl hydrolase family 3 with an apparent molecular mass of about 120 kDa. The purified 13-glucosidase showed optimal activity at pH 5.0 and 65℃ and was very stable at 50℃. Moreover, the enzyme exhibited good stability over pH 3.0-8.0 and possessed high tolerance towards pepsin and trypsin. The kinetic parameters Km （apparent Michaelis- Menten constant） and Vmax （maximal reaction velocity） for p-nitrephenyl-β-D-cjlucopyranoside （pNPG） were 1.73 mmol/L and 42.37 U/mg, respectively. The Krn and Vmax for cellobiose were 4.11 mmol/L and 5.7 U/mg, respectively. The enzyme efficiently converted isoflavone glycosides to aglycones, with a hydrolysis rate of 95.8% for daidzin, 86.7% for genistin, and 72.1% for glycitin. Meanwhile, the productivities were 1.14 mmol/（L.h） for daidzein, 0.72 mmol/（L.h） for genistein, and 0.19 mmol/（L.h） for glycitein. This is the first report on the application of A. terreus β-glucosidase for converting isoflavone glycosides to their aglycones in soybean products.

关键词： β-glucosidase Aspergillus terreus Characterization Hydrolysis Soybean isoflavones

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Remark on Mild Solution to the 3D Incompressible Micropolar System in Fourier–Herz Framework

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Acta Mathematica Sinica,English Series 2019年第10期35卷 1595-1616页

作者： Yao nie Xiao xin zheng School of Mathematics and Systems Science Beihang University

This paper is devoted to investigating the existence and uniqueness of mild solution to the 3D incompressible micropolar fluid system in the Fourier–Herz framework. By taking advantage of microlocal analysis and the mutual effect in the same frequency range of convection term, we give a special initial data(u0,ω0) whose norm of FB1,q^-1(q>2) is arbitrarily small, however, the couple(u0,ω0) produces a solution which is arbitrarily large in FB1,q^-1 after an arbitrarily short time. This implies the system is ill-posed in the sense of "norm inflation" as q>2.

关键词： Ill-posedness micropolar system Fourier-Herz space norm inflation

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Simulation and evaluation study of atmospheric aerosol nonsphericity as a function of particle size

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Particuology 2024年第7期90卷 281-291页

作者： Qianjun Mao xin nie School of Urban Construction Wuhan University of Science and TechnologyWuhan430065China College of New Energy and Materials Northeast Petroleum UniversityDaqing163318China

Aerosol nonsphericity causes great uncertainty in radiative forcing assessments and climate *** considerable studies have attempted to quantify this uncertainty,the relationship between aerosol nonsphericity and particle size is usually not considered,thus reducing the accuracy of the *** this study,a coupled inversion algorithm combining an improved stochastic particle swarm optimization algorithm and angular light scattering is used for the nonparametric estimation of aerosol nonsphericity variation with particle size,and the optimal sample selection method is employed to screen the *** on the verification of inversion accuracy,the variation of aerosol aspect ratio with particle size based on the ellipsoidal model in global regions has been obtained from Aerosol Robotic Network(AERONET)data,and the effect of nonsphericity on radiative forcing and dry deposition has been *** results show that the aspect ratio increases with particle size in all regions,with the maximum ranging from 1.4 to 1.8 in the desert,reflecting the differences in aerosol composition at different particle *** radiation calculations,considering aerosol nonsphericity makes the aerosol cooling effect weaker and surface radiative fluxes increase,but hardly changes the aerosol absorption,with maximum differences of 9.22%and 22.12%at the bottom and top of the atmosphere,***,the differences in radiative forcing between aspect ratios as a function of particle size and not varying with particle size are not significant,averaging less than 2%.Besides,the aspect ratio not varying with particle size underestimates the deposition velocity of small particles and overestimates that of large particles compared to that as a function of particle size,with maximum differences of 7%and 4%,respectively.

关键词： Aerosol nonsphericity Inversion Aspect ratio Radiative forcing Dry deposition velocity

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Robot Intelligence for Real World Applications

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Chinese Journal of Electronics 2018年第3期27卷 446-458页

作者： LIU Yunhui zheng Fan GUO Ruibin WANG Jiangliu nie Qiang WANG xin WANG Zerui T Stone Robotics Institute The Chinese University of Hong Kong National University of Defense Technology

This paper presents a brief review on recent work on machine intelligence for real-world applications of *** act in a real world environment,a robot should possess a broad sense of intelligence including speech,perception,reasoning,action,*** this paper,we particularly deal with the intelligence involving action or body *** intelligence related to robot action/motion can be classified into two categories:manipulation intelligence and mobility *** manipulation intelligence means the skill/intelligence of reliably manipulating objects according to tasks and the mobility intelligence corresponds to the ability of autonomously moving,or flying,and or jumping in a natural ***-robot interaction is another important topic for real-world *** addition to reviewing the ma jor approaches,this paper also gives an overview on our efforts in these important topics.

关键词： Machine intelligence Simultaneous localization and mapping(SLAM) Medical robots Humanrobot interaction

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Scattering Analysis and Optimization of Spherical Acoustic Cloak with Unideal Pentamode Material

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Acta Mechanica Solida Sinica 2020年第3期33卷 347-360页

作者： xin nie Yi Chen Xiaoning Liu Key Laboratory of Dynamics and Control of Flight Vehicle Ministry of EducationSchool of Aerospace EngineeringBeijing Institute of TechnologyBeijing 100081China

The acoustic scattering is theoretically studied in this paper for three-dimensional spherical cloak composed of unideal pentamode material,for which small shear rigidity is always inevitable for a real designed microstructure.A theoretical formulation is developed to efficiently evaluate the cloaking *** generic scattering feature of the cloak and the efTects of material imperfectness and inner cloak boundary constraints are systematically *** preferable constraint type and the critical imperfectness parameter of the material are identified for possible broadband *** addition,a very practical lining shell scheme is proposed to tune the constraint strength on the inner *** combining the theoretical model with optimization algorithm,it is further proved that the cloak can be reduced by several piecewiseuniform layers and optimized to achieve excellent invisibility in targeted frequency *** study will provide valuable guidance for the future microstructural design of cloaks.

关键词： Pentamode material Spherical acoustic cloak Scattering calculation Boundary effect Optimization

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Synthesis of SnO_2 nanorods from aqueous solution:The effect of preparation conditions on the formed patterns

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Chinese Chemical Letters 2010年第8期21卷 999-1002页

作者： xin Wen Huang Zong Jian Liu Yi Fan zheng Qiu Lin nie Resource & Environment Catalysis Institute College of Chemical Engineering and Materials Science Zhejiang University of Technology Hangzhou 310014 China College of Biological and Environmental Engineering Zhejiang University of Technology Hangzhou 310014 China College of Mechanical & Electrical Engineering Hangzhou Dianzi University Hangzhou 300018 China

SnO2 nanorods were deposited on the Si substrates in an aqueous solution containing both SnCl4 and CO（NH2）*** is found that different self-assembled patterns of SnO2 nanorods can be obtained by changing the deposition conditions such as the molar ratio of CO（NH2）2 to SnCl4 and the pretreatment of the *** SnO2 nanorods,for example,can be changed into flower-like patterns when the molar ratio of CO（NH2）2 to SnCl4 is raised,and well-aligned nanorod arrays can be formed when the pretreatment of the substrate is *** addition,some interesting patterns,***-like patterns,can also be observed.

关键词： Tin dioxide Nanorods Heterogeneous nucleation

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Au nanostructures:an emerging prospect in cancer theranostics

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Science China(Life Sciences) 2012年第10期55卷 872-883页

作者： nie xin CHEN ChunYing CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety National Center for Nanoscience and Technology of ChinaBeijing 100190China

Au nanoparticles have been used in biomedical applications since ancient times. However, the rapid development of nanotechnology over the past century has led to recognition of the great potential of Au nanoparticles in a wide range of applications. Advanced fabrication techniques allow us to synthesize a variety of Au nanostructures possessing physiochemical properties that can be exploited for different purposes. Functionalization of the surface of Au nanoparticles further eases their application in various roles. These advantages of Au nanoparticles make them particularly suited for cancer treatment and diagnosis. The small size of Au particles enables them to preferentially accumulate at tumor sites to achieve in vivo targeting after systemic administration. Efficient light absorption followed by rapid heat conversion makes them very promising in photothermal therapy. The facile surface chemistry of Au nanoparticles eases delivery of drugs, ligands or imaging contrast agents in vivo. In this review, we summarize recent development of Au nanoparticles in cancer theranostics including imaging-based detection, photothermal therapy, chemical therapy and drug delivery. The multifunctional nature of Au nanoparticles means they hold great promise as novel anti-cancer therapeutics.

关键词： Au nanoparticle anti-tumor activity photothermal therapy drug delivery surface plasmon resonance diagnostics

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Effect of replacing antibiotics using multi-enzyme preparations on production performance and antioxidant activity in piglets

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Journal of Integrative Agriculture 2017年第3期16卷 640-647页

作者： HAN xin-yan YAN Feng-ying nie xin-zheng XIA Wei CHEN Sha ZHANG Xiao-xu QIAN Li-chun Key Laboratory of Animal Nutrition and Feed Science in East China Ministry of Agriculture/College of Animal Sciences Zhejiang University Hangzhou 310058 P.R. China

The study was conducted to investigate the effects of replacing antibiotics using multi-enzyme preparations on growth performance,coefficient of total tract apparent digestibility,digestive enzyme activity,and antioxidant property in piglets.A total of 160 piglets（（21.35±0.22）kg）were randomly assigned to five dietary treatments：1）basal diet supplemented with antibiotics（AC）,2）antibiotic diet supplemented with 0.5 g kg^-1 multi-enzyme preparations（AC＋0.5EP）,3）antibiotic diet supplemented with 1.5 g kg^-1 multi-enzyme preparations（AC＋1.5EP）,4）basal diet supplemented with a half dosage of antibiotics and 1.5 g kg^-1 multi-enzyme preparations（AH＋1.5EP）,and 5）basal diet supplemented with 1.5 g kg^-1 multienzyme preparations（BC＋1.5EP）.The results showed that AC＋1.5EP significantly improved the feed efficiency,apparent digestibility of ether extract（EE）and crude ash（CA）,lipase activity in pancreas and duodenum content,maltase and lactase activity in jejunum and ileum mucosa,glutathione peroxidase（GSH-Px）concentration in serum and liver,and decreased malondialdehyde（MDA）concentration in serum and liver compared with piglets receiving AC（P〈0.05）.Piglets receiving BC＋1.5EP showed no significant difference in growth performance（P〉0.05）but had lower MDA concentration than piglets receiving AC（P〈0.05）.The apparent digestibility of EE and crude fiber（CF）,duodenal lipase activity,jejunum mucosa maltase,and ileum mucosa lactase activity of piglets receiving AH＋1.5EP or BC＋1.5EP were significantly improved compared with piglets receiving AC（P〈0.05）.These results indicated an additive growth promotion effect between antibiotics and multi-enzyme preparations on piglets,and the multi-enzyme preparations may be used as substitutes for antibiotics for improving piglet production performance and health status.

关键词： antibiotics multi-enzyme preparations growth performance digestibility antioxidant property

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Investigation on the spatial-polarizational correlation based on 3GPP spatial channel model

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Science China(Information Sciences) 2014年第2期57卷 80-91页

作者： nie xin ZHANG JianHua ZHANG Ping Beijing Institute of Spacecraft System Engineering Key Laboratory of Universal Wireless Communications Ministry of EducationBeijing University of Posts and Telecommunications

The correlation among signals arriving at diferent elements of the antenna array has a significant impact on the performance of multiple-input multiple-output(MIMO)*** investigate the inter-element correlation when slant polarized antennas are used,a general approach for calculating the correlation coefcient is proposed based on a model that incorporates the antenna configuration,namely the antenna spacing and the slant angle,as well as the channel parameters,such as the power azimuth spectrum(PAS)and the crosspolarization discrimination(XPD).By applying this method to the 3rd generation partnership project(3GPP)spatial channel model(SCM),the expression of the inter-element correlation when the PAS follows the Laplacian distribution is *** approximate expressions are also given for two special cases,i.e.,the case where the angular spread(AS)of the PAS is small,and the case where the AS is so large that the PAS can be approximated by the uniform *** these expressions,the impact of the antenna configuration and the channel parameters on the correlation is *** that,the trend of inter-element correlation with the slant angle is ***,the equivalent relation between the slant angle and the antenna spacing is *** results can provide guidelines for the antenna configuration under diferent propagation conditions.

关键词： inter-element correlation slant polarized antennas power azimuth spectrum cross-polarization discrimination

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