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Study on Chemical Cross-linking Modification of Hyaluronan and the Biocompatibility of its Derivatives

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Chinese Journal of Biomedical Engineering(English Edition) 2006年第4期15卷 139-150页

作者： HU Guo-ying LIU xin GU han-qing The Second Hospital of Tianjin Medical University 300211 TianjinChina The Second Hospital of Tianjin Medical University300211 TianjinChina The Second Hospital of Tianjin Medical University300211 TianjinChina

Objective: Prepare cross-linked HA gels with higher mechanical stability, lower degradation velocity and desirable biocompatibility,so as to extend the usage of ***:*** molecular weight of HA (Mr_ HA ) by viscosimetry;*** cross-linked HA gels by DVS, GTA, DEC;*** the cross-linking and degradation procedure;*** the biocompatibility of the best HA gels. Results:The mechanical stability and durability to degradation of HA-DVS gels are superior to those of other gels, and when HA:DVS = 40:1(g/g), at 35℃ and in 0.2 M NaOH solution, the HA-DVS gel shows the best mechanical stability, and its cytotoxicity reaches class I, hemolysis ratio is lower than 5%. Conclusion:Our HA-DVS gel can be used to prepare biologic scaffolds.

关键词： Hyaluronic acid (Hyaluronan) Cross-linking Divinyl sulfone Diepoxy compound Glutaraldehyde Biocompatibility In vitro degradation

Study on the Preparation of Decellularized Scaffold of Bovine Pericardium

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Chinese Journal of Biomedical Engineering(English Edition) 2010年第3期19卷 129-138页

作者： YAN Fei-yan WEI Yi-han LIU xin GU han-qing Department of Biomedical Engineering Tianjin Medical University Tianjin 300211 China

Objective： To search for the best procedure on preparation ofacellular bovine pericardium,so to provide scaffolds for constructing tissue-engineering Methods： The bovine pericardiums were treated with 5 methods, which were divided into 6 groupsGroup A： Fresh bovinepericardium; Group B： Trypsin-detergent group;Group C： Freeze-thaw-detergent 24 h group; Group I）： Freeze-thaw-detergent 48 h group; Group Fz Freeze-thaw-nuclease group; Group F： Detergent-nuclease group. Then, by HE staining and scanning electron microscope to observe the effects ofdecellularization and fibrous changes among the 6 groups; by water content testingonechanical testing to observe the changes in physical properties of the matrix; by detecting the DNA content of each group to determine the effect of deeellularization qualitatively; by eytotoxieity test to detect the biocompatibility of bovine pericardium in each group. Results： The 5 methods can all remove the cellular components effectively, compared with the fresh bovine pericardium,the water content of each deeellularized group were increased （P〈0.05）, while the DNA content decreased （P〈0.05）, with statistically significant differences. Of group E, the fibers were a little disorder, with the largest tension and the elastic modulus increased, while the rupture tensile rate decreased. Compared with fresh bovine pericardium,the largest tension of the other decellularization groups were all decreased （P〈0.05）. The fibers of group B, group D were irregularly arranged and also with ruptures, both the elastic modulus and the rupture tensile rate decreased（P〈0.05）. In group C and F, the fibers were dense and their direction was normal, the elastic modulus and the rupture tensile rate were similar to the fresh bovine pericardium （P〉0.05）. Cytotoxicity results showed that the cell toxicity of group B, group C, group D, group E and group F were respectively 0.9, 0.6, 1.0, 1.0 and 0.5, each group were qualified toxicity test, in which group C and group

关键词： bovine pericardium deeellularization freeze-thaw method scaffold detergent

Breaking the structural symmetry of active sites in zeolite imidazolate framework for enhanced CO2 photoreduction

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Science China Chemistry 2025年

作者： Yupeng han Yayu Yan Qiaohong Li xin Wu Fei Wang Jian Zhang State Key Laboratory of Structural Chemistry Fujian Institute of Research on the Structure of Matter Chinese Academy of Sciences University of Chinese Academy of Sciences College of Chemistry Fuzhou University

Adjusting the structure and composition of active sites is a highly effective method for activating linear ***, precise control of interfacial chemistry within molecular-scale environments presents a significant challenge in heterogeneous catalysis. In our study, we present symmetry-broken Mo-O-Co triple-atom active sites within the hybrid zeolite imidazole framework HZIF-CoMo. These sites disrupt the electronic balance and create a weak built-in electric field, thus promoting photocatalytic CO2reduction. The CO yield over HZIF-CoMo is improved to 3.1 mmol g-1h-1, which is over four times higher than the parent cobalt-imidazole framework MAF-6-Co. Both experimental and theoretical studies indicate that electronic modulation through the symmetry-breaking Mo-O-Co moiety acts as an organizing knob to accelerate charge separation and result in more carriers participating in the catalytic reaction. This work provides important insights into optimizing site-related electronic structures for targeted photocatalytic reactions.

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Single-cell omics: experimental workflow, data analyses and applications

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Science China Life Sciences 2025年第1期68卷 5-102页

作者： Fengying Sun Haoyan Li Dongqing Sun Shaliu Fu Lei Gu xin Shao Qinqin Wang xin Dong Bin Duan Feiyang xing Jun Wu Minmin Xiao Fangqing Zhao Jing-Dong J.han Qi Liu Xiaohui Fan Chen Li Chenfei Wang Tieliu Shi Department of Clinical Laboratory the Affiliated Wuhu Hospital of East China Normal University (The Second People's Hospital of Wuhu City) Pharmaceutical Informatics Institute College of Pharmaceutical SciencesZhejiang University Key Laboratory of Spine and Spinal Cord Injury Repair and Regeneration (Tongji University) Ministry of EducationOrthopaedic DepartmentTongji HospitalBioinformatics DepartmentSchool of Life Sciences and TechnologyTongji University Frontier Science Center for Stem Cells School of Life Sciences and TechnologyTongji University Translational Medical Center for Stem Cell Therapy and Institute for Regenerative Medicine Shanghai East HospitalBioinformatics DepartmentSchool of Life Sciences and TechnologyTongji University Research Institute of Intelligent Computing Zhejiang Lab Shanghai Research Institute for Intelligent Autonomous Systems Center for Single-cell Omics School of Public HealthShanghai Jiao Tong University School of Medicine National Key Laboratory of Chinese Medicine Modernization Innovation Center of Yangtze River DeltaZhejiang University Center for Bioinformatics and Computational Biology Shanghai Key Laboratory of Regulatory Biologythe Institute of Biomedical Sciences and School of Life SciencesEast China Normal University Beijing Institutes of Life Science Chinese Academy of Sciences Peking-Tsinghua Center for Life Sciences Academy for Advanced Interdisciplinary StudiesCenter for Quantitative Biology (CQB)Peking University Zhejiang Key Laboratory of Precision Diagnosis and Therapy for Major Gynecological Diseases Women's HospitalZhejiang University School of Medicine Key Laboratory of Advanced Theory and Application in Statistics and Data Science-MOE School of StatisticsEast China Normal University

Cells are the fundamental units of biological systems and exhibit unique development trajectories and molecular features. Our exploration of how the genomes orchestrate the formation and maintenance of each cell, and control the cellular phenotypes of various organismsis, is both captivating and intricate. Since the inception of the first single-cell RNA technology, technologies related to single-cell sequencing have experienced rapid advancements in recent years. These technologies have expanded horizontally to include single-cell genome, epigenome,proteome, and metabolome, while vertically, they have progressed to integrate multiple omics data and incorporate additional information such as spatial sc RNA-seq and CRISPR screening. Single-cell omics represent a groundbreaking advancement in the biomedical field,offering profound insights into the understanding of complex diseases, including cancers. Here, we comprehensively summarize recent advances in single-cell omics technologies, with a specific focus on the methodology section. This overview aims to guide researchers in selecting appropriate methods for single-cell sequencing and related data analysis.

关键词： single-cell sequencing genome epigenome proteomics metabolomics multimodal spatial transcriptomics CRISPR screening

Advances in ethnopharmacology,phytochemistry and pharmacology of Erigerontis Herba and its Chinese medicine prescriptions

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Traditional Medicine Research 2025年第1期10卷 44-61页

作者： Yang Zhou Yuan-Yan Huang Ding-Yue Shang Ying Chen Meng Tian Cheng-Yu Jin xin Lu Bing-Chun Yan han-qing Pang Institute of Translational Medicine School of MedicineYangzhou UniversityYangzhou 225009China Jiangsu Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Treatment of Senile Diseases Yangzhou UniversityYangzhou 225009China School of Medicine Nanjing University of Chinese MedicineNanjing 210023China State Key Laboratory for Chemistry and Molecular Engineering of Medicinal Resources Guangxi Normal UniversityGuiling 541004China.

Erigerontis Herba(EH),the dried whole plant of Erigeron breviscapus,is well-known for circulating blood,activating meridians to alleviate pain,expelling wind,and clearing away *** has been extensively utilized in southern China for the treatment of stroke hemiplegia,chest stuffiness and pains,rheumatic arthralgia,headache,and *** review focuses on the botany,ethnopharmacology,phytochemistry,pharmacology and toxicity of EH and its related prescriptions to offer new insights for prospective research of *** information about EH was retrieved from ancient records and books,PubMed,China National Knowledge Infrastructure,Chinese Pharmacopoeia,Web of Science,Doctoral and Master’s Theses,and various electronic *** is a member of Compositae family and is mainly grown in southern *** Chinese medicine records that EH has the effects of circulating blood and removing blood stasis,expelling wind,and removing cold,as well as relieving rigidity of muscle and relieving *** now,nearly 200 ingredients have been characterized from EH,including flavonoids,caffeoyls,aromatic acids,coumarins,pentacyclic terpenoids,volatile oil and other *** extracts,EH related prescriptions(Dengzhan Xixin injection,Dengzhan Shengmai capsules,etc.)or compounds(scutellarin,scutellarein,etc.)possessed obvious therapeutic effects of ischemic stroke,cerebral hemorrhage,myocardial infarction,Alzheimer’s disease,diabetes and its complications,gastric cancer,bone,and joint degenerative ***,the major active compound of EH,has been used as a quality *** no obvious toxicity of EH has been *** to its traditional applications,ethnopharmacology,phytochemistry,pharmacology,and toxicity,EH was applied as a valuable herb for clinical application in food and medicine *** several compounds have been shown to possess diverse biological activities,the underlying mechanisms of their actions remain *** fully exploi

关键词： Erigeron breviscapus traditional applications ethnopharmacology phytochemistry pharmacology

Trinuclear gold-catalyzed site-selective alkylation of peptides

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Science China Chemistry 2025年第1期 249-256页

作者： qing-Yun Fang Chengyihan Gu Yinghan Chen Wencheng Yan Yong Liang Weipeng Li Chengjian Zhu Jie han Jin Xie State Key Laboratory of Coordination Chemistry Jiangsu Key Laboratory of Advanced Organic Materials Chemistry and Biomedicine Innovation Center (Chem BIC) School of Chemistry and Chemical Engineering Nanjing University Green Catalysis Center College of Chemistry Zhengzhou University State Key Laboratory of Natural Medicines China Pharmaceutical University

Selective modification of peptides has always been a challenging issue. The selective alkylation of peptides can alter the biological activity and physical properties of molecules, and it has gained significant research interest. We herein disclose an intriguing gold-catalyzed alkylation of peptides using bench-stable unactivated alkyl bromides under photoredox catalysis. A wide range of structurally diverse primary, secondary and tertiary alkyl bromides serve as effective coupling partners to precisely connect with the α-C(sp3)–H in glycine residue of peptides. This protocol demonstrates an exceptionally broad scope for alkyl halides and excellent tolerance for a wide range of useful functional groups. A series of relay transformations to construct cyclic peptides, to proceed click reaction and to realize peptide fluorescence labeling further enhance its synthetic practicality. In addition, mechanistic studies and density functional theory(DFT) calculations reveal an inner-sphere and subsequent outersphere single electron transfer(SET) mechanism.

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轴压作用下双层碳纳米管屈曲问题的理论分析

暨南大学学报（自然科学与医学版） 2005年第1期26卷 54-56页

作者：梁颖晶黄小清辛浩韩强华南理工大学交通学院工程力学系广东广州510640

　考虑双层碳纳米管层间范德华力和弹性介质的作用,利用弹性梁理论,建立了轴压作用下弹性介质中碳纳米管屈曲问题的理论分析模型,并给出了相应的临界屈曲应力.

关键词：纳米管屈曲范德华力

183例次支气管热成形术后近期呼吸不良事件分析

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中华结核和呼吸杂志 2017年第3期40卷 176-181页

作者：农英林江涛苏楠陈昕赵青韩春燕裘若帆中日友好医院呼吸与危重症医学科北京100029

目的分析支气管热成形术（BT）治疗重症支气管哮喘术后近期（术后3周）呼吸不良事件发生情况.方法 2014年3月至2016年7月中日友好医院62例重症哮喘患者接受BT治疗,共完成183例次手术.收集术后3周内所有患者呼吸不良事件发生情况,分析... 详细信息

目的分析支气管热成形术（BT）治疗重症支气管哮喘术后近期（术后3周）呼吸不良事件发生情况.方法 2014年3月至2016年7月中日友好医院62例重症哮喘患者接受BT治疗,共完成183例次手术.收集术后3周内所有患者呼吸不良事件发生情况,分析可能影响术后不良事件发生的因素.结果术后3周内62例患者中43例（69.4%）出现呼吸不良事件,87例次手术（47.5%）发生不良事件共153例次.主要不良事件为咳嗽（15例次,8.20%）、咳痰量增加（37例次,20.22%）、呼气峰值流量（PEF）短暂下降（37例次,20.22%）、胸闷（12例次,6.56%）、痰中带血丝（11例次,6.01%）、哮喘急性发作（10例次,5.46%）、肺炎（6例次,3.28%）.多数不良事件在术后1周可自行缓解或经对症治疗消失.无气管插管、恶性心律失常及死亡等严重不良事件发生.患者基础诱导痰嗜酸粒细胞百分比、基础血嗜酸粒细胞百分比、手术次数及术前FEV1占预计值%会影响术后不良事件的发生.术前FEV1占预计值%≥60%可减少术后不良事件发生.结论应用支气管热成形术治疗重症哮喘近期安全性良好.

关键词：哮喘治疗学支气管热成形术不良反应

Facile synthesis of the dodecahydridododecaborate (B12H122-) from borane Lewis base adducts

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Science China Chemistry 2025年

作者： Yi Jing xin-Ran Liu xing-Hua Wang Hui han Sha-Sha Liang Xi-Meng Chen Dong-Hui Wei Lai-Sheng Wang Xue-Nian Chen College of Chemistry Zhengzhou University School of Chemistry and Chemical Engineering Henan Key Laboratory of Boron Chemistry and Advanced MaterialsHenan Normal University Department of Chemistry Brown University

Polyhedral boranes are a class of well-known boron molecular clusters widely used in energy,chemistry,medicine,and materials science because of their unique physical and chemical *** efforts have been made in the past decades to find more effective synthetic methods for this important class of boron ***,existing synthetic methods suffer from low efficiency and low ***,we report a facile one-pot synthesis of[（CH3）3S]2B12H12with moderate yields at mild *** mechanisms for the multi-step chemoselective synthesis of B12H122-without other by-products are elucidated based on theoretical results and our previous *** Lewis base used in B–H bond condensation reaction,which acts as a hydrogen or to balance the newly generated polyhedral borane charges,is proposed and studied in *** current study has led to a more effective and selective synthetic method for B12H122-and has also implicated the syntheses of other new polyhedral boranes.

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