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PREPARATION OF ENANTIOMERICALLY PURE SYN-4,5-DIHYDROXY CARBOXYLIC ACID LACTONES BY MICROBIAL REDUCTION

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Chinese Chemical Letters 1991年第5期2卷 345-346页

作者： jian xin gu Zu Yi LI guo Qiang LIN Shanghai Institute of Organic Chemistry Academia Sinica 345 Lingling LuShanghai 200032

Enantiomerically pure syn-4,5-dihydroxy carboxylic acid lactones were prepared by microbial reduction of acyl lactones with resting cell of Aspergillus niger.

关键词： SYN PREPARATION OF ENANTIOMERICALLY PURE SYN-4,5-DIHYDROXY CARBOXYLIC ACID LACTONES BY MICROBIAL REDUCTION ACID

BIOTRANSFORMATION OF α-DIKETONES-SYNTHESIS OF OPTICALLY ACTIVE α-KETOLS

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Chinese Chemical Letters 1995年第6期6卷 457-458页

作者： jian xin gu Zu Yi Li guo Qiang Lin(Shanghai Institute of Organic Chemistry,Chinese Academyof Sciences,Fenglin Lu 354,Shanghai 200032) Chinese Acad Sci Shanghai Inst Organ Chem Shanghai 200032 Peoples R China

Asymmetric reduction of some α-diketones such as 1-phenyl-1,2-propanedione and substituted benzils with fungus Geotrichum sp.G38 afforded corresponding optically edhe α-ketols in excellent chemical and optical yields.

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Analysis of the urinary peptidome associated with Helicobacter pylori infection

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World Journal of Gastroenterology 2011年第5期17卷 618-624页

作者： Di Xiao Fan-Liang Meng Li-Hua He Yi-xin gu jian-Zhong Zhang National Institute for Communicable Disease Controland Prevention Chinese Center for Disease Control and Pre-vention Beijing 102206 China

AIM： To investigate the relationship between urinary peptide changes and Helicobacter pylori （H. pylorl） infection using urinary peptidome profiling. METHODS： The study was performed in volunteers （n = 137） who gave informed consent. Urinary peptides were enriched by magnetic beads based weak cation exchange chromatography＇and spectrums acquired by matrix-assisted laser desorption/ionization time-of-flight （MALDI-TOF） mass spectrometry （MS）. ClinProTools bioinformatics software was used for statistical analysis and the recognition of peptide patterns. The marker peptides were identified by LTQ Obitrap XL tandem MS. RESULTS： Approximately 50 proteins or peptides which loaded onto the magnetic beads were detected by MAL-DI-TOF MS. By optimizing the parameters of the model, the Genetic Algorithm model had good recognition capability （97%） and positive predictive value （94%）. Based on the model, 2 markers with molecular masses of 6788 and 1912 Da were found that differentiated between H. pylori positive and negative volunteers. The m/z 1912 sequence was parsed as SKQFTSSTSYN- RGDSTF. The peptide was identified as isoform 1 of the fibrinogen a chain precursor, whose concentration in urine was markedly higher in H. pylori infected volunteers than in H. pylori non-infected ones. CONCLUSION： The appearance of urinary fibrinogen degradation products is caused by an active H. pyloriinduced process.

关键词： Urinary peptidome profiling MB-matrix-assisted laser desorption/ionization time-of-flight massspectrometry Helicobacter pylori Fibrinogen degrada-tion products

Speed-regulating Simulation of Electronic Governor of Marine Main Diesel Engine

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Speed-regulating Simulation of Electronic Governor of Marine...

支撑方式对曲轴渗氮过程畸变影响的有限元分析

作者： CHU jian-xin, gu Wei, ZHENG Shi-jun (Marine SimulationCenter, Shanghai Maritime University,Shanghai,200135, China)

＜正＞This paper takes SPC-33 electronic governor as a research object, deduces its mathematics model and puts forward its simulative calculation block diagram. A visualized simulation software of electronic governor ... 详细信息

关键词： electronic governor speed regulation simulation

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上海交通大学学报 2003年第2期37卷 194-197页

作者：姚新顾剑锋李勇军潘健生上海交通大学材料科学与工程学院上海200030

对大型柴油机曲轴在渗氮过程中因支撑方式的不同而引起的曲轴畸变进行了有限元模拟分析 .实际测试了曲轴用 35Cr Mo钢在 50 0°C时的弹塑性和粘性力学参数 ,并运用陈化理论模型回归出应变与应力随时间变化的函数关系 .基于粘弹性有限元... 详细信息

对大型柴油机曲轴在渗氮过程中因支撑方式的不同而引起的曲轴畸变进行了有限元模拟分析 .实际测试了曲轴用 35Cr Mo钢在 50 0°C时的弹塑性和粘性力学参数 ,并运用陈化理论模型回归出应变与应力随时间变化的函数关系 .基于粘弹性有限元模型对 4种可能支撑方式下曲轴在50 0°C渗氮 50 h后的变形进行模拟 ,并选出了最佳的支撑方案 .实际测试结果表明 ,模拟结果基本反映了变形状况 .将其应用于实际生产 ,解决了大型曲轴渗氮畸变控制的难题 .

关键词：渗氮粘弹性曲轴畸变有限元

Comparative proteomic analysis of plasma exosomes reveals the functional contribution of N-acetyl-alpha-glucosaminidase to Parkinson’s disease

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Neural Regeneration Research 2025年第10期20卷 2998-3012页

作者： Yuan Zhao Yidan Zhang xin Liu jian Zhang Ya Gao Shuyue Li Cui Chang Xiang Liu guofeng Yang Department of Geriatrics Second Hospital of Hebei Medical UniversityShijiazhuangHebei ProvinceChina Department of Neurosurgery The Second Hospital of Hebei Medical UniversityShijiazhuangHebei ProvinceChina

Parkinson’s disease is the second most common progressive neurodegenerative disorder,and few reliable biomarkers are available to track disease *** proteins,DNA,mRNA,and lipids carried by exosomes reflect intracellular changes,and thus can serve as biomarkers for a variety of *** this study,we investigated alterations in the protein content of plasma exosomes derived from patients with Parkinson’s disease and the potential therapeutic roles of these proteins in Parkinson’s *** a tandem mass tag-based quantitative proteomics approach,we characterized the proteomes of plasma exosomes derived from individual patients,identified exosomal protein signatures specific to patients with Parkinson’s disease,and identified N-acetyl-alpha-glucosaminidase as a differentially expressed protein.N-acetyl-alpha-glucosaminidase expression levels in exosomes from the plasma of patients and healthy controls were validated by enzyme-linked immunosorbent assay and western *** results demonstrated that the exosomal N-acetyl-alpha-glucosaminidase concentration was not only lower in Parkinson’s disease,but also decreased with increasing Hoehn-Yahr stage,suggesting that N-acetyl-alpha-glucosaminidase could be used to rapidly evaluate Parkinson’s disease ***,western blot and immunohistochemistry analysis showed that N-acetyl-alpha-glucosaminidase levels were markedly reduced both in cells treated with 1-methyl-4-phenylpyridinium and cells overexpressingα-synuclein compared with control ***,N-acetyl-alpha-glucosaminidase overexpression significantly increased cell viability and inhibitedα-synuclein expression in 1-methyl-4-phenylpyridinium-treated *** together,our findings demonstrate for the first time that exosomal N-acetyl-alpha-glucosaminidase may serve as a biomarker for Parkinson’s disease diagnosis,and that N-acetyl-alpha-glucosaminidase may reduceα-synuclein expression and 1-methyl-4-phenylpyridinium-induced n

关键词： biomarker diagnosis exosomes N-acetyl-alpha-glucosaminidase Parkinson’s disease proteomic α-synuclein

Breaking the structural symmetry of active sites in zeolite imidazolate framework for enhanced CO2 photoreduction

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Science China Chemistry 2025年

作者： Yupeng Han Yayu Yan Qiaohong Li xin Wu Fei Wang jian Zhang State Key Laboratory of Structural Chemistry Fujian Institute of Research on the Structure of Matter Chinese Academy of Sciences University of Chinese Academy of Sciences College of Chemistry Fuzhou University

Adjusting the structure and composition of active sites is a highly effective method for activating linear ***, precise control of interfacial chemistry within molecular-scale environments presents a significant challenge in heterogeneous catalysis. In our study, we present symmetry-broken Mo-O-Co triple-atom active sites within the hybrid zeolite imidazole framework HZIF-CoMo. These sites disrupt the electronic balance and create a weak built-in electric field, thus promoting photocatalytic CO2reduction. The CO yield over HZIF-CoMo is improved to 3.1 mmol g-1h-1, which is over four times higher than the parent cobalt-imidazole framework MAF-6-Co. Both experimental and theoretical studies indicate that electronic modulation through the symmetry-breaking Mo-O-Co moiety acts as an organizing knob to accelerate charge separation and result in more carriers participating in the catalytic reaction. This work provides important insights into optimizing site-related electronic structures for targeted photocatalytic reactions.

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Single-cell omics: experimental workflow, data analyses and applications

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Science China Life Sciences 2025年第1期68卷 5-102页

作者： Fengying Sun Haoyan Li Dongqing Sun Shaliu Fu Lei gu xin Shao Qinqin Wang xin Dong Bin Duan Feiyang xing Jun Wu Minmin Xiao Fangqing Zhao Jing-Dong J.Han Qi Liu Xiaohui Fan Chen Li Chenfei Wang Tieliu Shi Department of Clinical Laboratory the Affiliated Wuhu Hospital of East China Normal University (The Second People's Hospital of Wuhu City) Pharmaceutical Informatics Institute College of Pharmaceutical SciencesZhejiang University Key Laboratory of Spine and Spinal Cord Injury Repair and Regeneration (Tongji University) Ministry of EducationOrthopaedic DepartmentTongji HospitalBioinformatics DepartmentSchool of Life Sciences and TechnologyTongji University Frontier Science Center for Stem Cells School of Life Sciences and TechnologyTongji University Translational Medical Center for Stem Cell Therapy and Institute for Regenerative Medicine Shanghai East HospitalBioinformatics DepartmentSchool of Life Sciences and TechnologyTongji University Research Institute of Intelligent Computing Zhejiang Lab Shanghai Research Institute for Intelligent Autonomous Systems Center for Single-cell Omics School of Public HealthShanghai Jiao Tong University School of Medicine National Key Laboratory of Chinese Medicine Modernization Innovation Center of Yangtze River DeltaZhejiang University Center for Bioinformatics and Computational Biology Shanghai Key Laboratory of Regulatory Biologythe Institute of Biomedical Sciences and School of Life SciencesEast China Normal University Beijing Institutes of Life Science Chinese Academy of Sciences Peking-Tsinghua Center for Life Sciences Academy for Advanced Interdisciplinary StudiesCenter for Quantitative Biology (CQB)Peking University Zhejiang Key Laboratory of Precision Diagnosis and Therapy for Major Gynecological Diseases Women's HospitalZhejiang University School of Medicine Key Laboratory of Advanced Theory and Application in Statistics and Data Science-MOE School of StatisticsEast China Normal University

Cells are the fundamental units of biological systems and exhibit unique development trajectories and molecular features. Our exploration of how the genomes orchestrate the formation and maintenance of each cell, and control the cellular phenotypes of various organismsis, is both captivating and intricate. Since the inception of the first single-cell RNA technology, technologies related to single-cell sequencing have experienced rapid advancements in recent years. These technologies have expanded horizontally to include single-cell genome, epigenome,proteome, and metabolome, while vertically, they have progressed to integrate multiple omics data and incorporate additional information such as spatial sc RNA-seq and CRISPR screening. Single-cell omics represent a groundbreaking advancement in the biomedical field,offering profound insights into the understanding of complex diseases, including cancers. Here, we comprehensively summarize recent advances in single-cell omics technologies, with a specific focus on the methodology section. This overview aims to guide researchers in selecting appropriate methods for single-cell sequencing and related data analysis.

关键词： single-cell sequencing genome epigenome proteomics metabolomics multimodal spatial transcriptomics CRISPR screening

SMMC7721肝癌细胞蛋白激酶B活性增高可驱动有功能的上皮钙粘着蛋白到细胞表面(英文)

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生物化学与生物物理进展 2003年第5期30卷 715-720页

作者：陈舌殷祥雷宗鸿亮范凯谊黄传新顾建新申宗侯复旦大学上海医学院生物化学系上海200032

为了研究蛋白激酶B (PKB)对上皮钙粘着蛋白 (E cadherin)的调节 ,使用了用胰岛素处理的野生型SMMC 772 1细胞及稳定表达持续激活PKB的SMMC 772 1细胞株 (Gag PKB/SMMC 772 1) .用RNA印迹法和蛋白质印迹法检测细胞E cadherin表达 ,发现... 详细信息

为了研究蛋白激酶B (PKB)对上皮钙粘着蛋白 (E cadherin)的调节 ,使用了用胰岛素处理的野生型SMMC 772 1细胞及稳定表达持续激活PKB的SMMC 772 1细胞株 (Gag PKB/SMMC 772 1) .用RNA印迹法和蛋白质印迹法检测细胞E cadherin表达 ,发现通过胰岛素刺激或在细胞中表达持续激活PKB从而增加PKB活性 ,不影响E cadherin的转录和蛋白质合成 ,但用流式细胞术和免疫荧光定位E cadherin ,则发现PKB活性增加能明显驱动E cadherin到细胞表面 ,从而导致部分通过E cadherin途径的细胞粘聚增加和细胞调亡的抑制 .因此 ,我们提供新的证据表明 ,增加PKB活性可驱动有功能的E cadherin分子到细胞表面 .

关键词：肝癌细胞蛋白激酶B 活性增高上皮钙粘着蛋白细胞表面细胞粘聚失巢凋亡