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Visual function and vision-related quality of life after vitrectomy for idiopathic macular hole: a 12mo followup study

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International Journal of Ophthalmology(English edition) 2015年第4期8卷 764-769页

作者： Hong-Tao Duan Song Chen yue-xin wang Jia-Hui Kong Meng Dong Clinical College of Ophthalmology Tianjin Medical University Tianjin Eye Hospital Tianjin Institute of Ophthalmology Tianjin Key Laboratory of Ophthalmology and Visual Sciences

· AIM: To investigate the visual function and the relationship with vision-related quality of life(VRQOL)after macular hole repair surgery.· METHODS: Prospective case series. Thirty-six consecutive eyes in 36 patients who underwent pars plana vitrectomy(PPV) and internal limiting membrane(ILM) peeling were included. The 25-item National Eye Institute Visual Function Questionnaire(VFQ-25) was answered by the participants before and 3 and 12 mo after operation. Follow-up visits examinations included best-corrected visual acuity(BCVA), clinical examination,and central macular thickness(CMT) measured by optical coherence tomography(OCT).·RESULTS: Macular-hole closure was achieved in 35 of36 eyes(97.2%). At baseline and months 3 and 12, the log MAR BCVAs(mean±SD) were 1.15±0.47, 0.68±0.53(P <0.0001 versus baseline), and 0.55 ±0.49(P <0.001 versus baseline, P =0.273 versus month 3), respectively; the CMTs(μm) were 330 ±81, 244 ±62(P <0.001 versus baseline), and 225±58(P <0.001 versus baseline, P =0.222 versus month 3), respectively; the median preoperative VFQ-25 composite score of 73.50(63.92-81.13) increased postoperatively to 85.50(80.04-89.63) at 3mo(P <0.001)and 86.73(82.50-89.63) at 12mo(P <0.001) *** improved BCVA was correlated with improvements in five subscales(r =-0.605 to-0.336, P <0.001 to P =0.046) at 12 mo.· CONCLUSION: PPV with ILM peeling improved anatomic outcome, visual function, and VRQOL. Theimproved BCVA was an important factor related to the improved VRQOL.

关键词： idiopathic macular hole vitrectomy quality of life National Eye Institute Visual Function Questionnaire

Fabrication and Characterization of NEMS

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Optoelectronics Letters 2007年第2期3卷 91-94页

作者： JIAO Ji-wei YANG Heng LI Tie LI xin-xin wang yue-lin State Key Laboratory of Transducer Technology Shanghai Institute of Microsystem and Information Technology（SIMIT） Chinese Academy of Sciences Shanghai 200050 China

We proposed a called“nano-fabrication based on MEMS technology”approach to realize the typical nano-electro- mechanical structures,such as integrated nano probe,ultrathin cantilever,silicon nano wire,and doubly clamped nano beam, to demonstrate the feasibility and *** also introduced the characterization of nano structures based on laser vibrometer and piezoresistive effect,the latter method was first time applied to investigate a doubly daped nanobeam with a thickness of about 200 nm.

关键词： NEMS 纳米级机电结构制造特征 MEMS技术

Increased endothelin receptor B and G protein coupled kinase-2 in the mesentery of portal hypertensive rats

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World Journal of Gastroenterology 2013年第13期19卷 2065-2072页

作者： Qing-Hong Du Lin Han Jun-Jie Jiang Peng-Tao Li xin-yue wang Xu Jia Department of Pathology School of Fundamental MedicineBeijing University of Chinese Medicine

AIM: To elucidate the mechanisms of mesenteric vasodilation in portal hypertension (PHT), with a focus on endothelin signaling. METHODS: PHT was induced in rats by common bile duct ligation (CBDL). Portal pressure (PP) was measured directly via catheters placed in the portal vein tract. The level of endothelin-1 (ET-1) in the mesenteric circulation was determined by radioimmunoassay, and the expression of the endothelin A receptor (ETAR) and endothelin B receptor (ETBR) was assessed by immunofluorescence and Western blot. Additionally, expression of G protein coupled kinase-2 (GRK2) and β-arrestin 2, which influence endothelin receptor sensitivity, were also studied by Western blot. RESULTS: PP of CBDL rats increased significantly (11.89 ± 1.38 mmHg vs 16.34 ± 1.63 mmHg). ET-1 expression decreased in the mesenteric circulation 2 and 4 wk after CBDL. ET-1 levels in the systemic circulation of CBDL rats were increased at 2 wk and decreased at 4 wk. There was no change in ETAR expression in response to CBDL; however, increased expression of ETBR in the endothelial cells of mesenteric arterioles and capillaries was observed. In sham-operated rats, ETBR was mainly expressed in the CD31+ endothelial cells of the arterioles. With development of PHT, in addition to the endothelial cells, ETBR expression was noticeably detectable in the SMA+ smooth muscle cells of arterioles and in the CD31+ capillaries. Following CBDL, increased expression of GRK2 was also found in mesenteric tissue, though there was no change in the level of β-arrestin 2. CONCLUSION: Decreased levels of ET-1 and increased ETBR expression in the mesenteric circulation following CBDL in rats may underlie mesenteric vasodilation in individuals with PHT. Mechanistically, increased GRK2 expression may lead to desensitization of ETAR, as well as other vasoconstrictors, promoting this vasodilatory effect.

关键词： Portal hypertension Mesentery Endothelin Endothelin B receptor G protein coupled kinase-2

A comparative study on the transplantation of different concentrations of human umbilical mesenchymal cells into diabetic rats

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International Journal of Ophthalmology(English edition) 2015年第2期8卷 257-262页

作者： Jia-Hui Kong Dan Zheng Song Chen Hong-Tao Duan yue-xin wang Meng Dong Jian Song Clinical College of Ophthalmology Tianjin Medical University Tianjin Eye Hospital Tianjin Institute of Ophthalmology Tianjin Key Laboratory of Ophthalmology and Visual Sciences

AIM: To observe the effects of intravitreal injections of different concentrations of human umbilical mesenchymal stem cells on retinopathy in rats with diabetes ***: Healthy and adult male Sprague-Dawley(SD) rats were randomly assigned to a normal control group(group A), a diabetic retinopathy(DR) blank control group(group B), a high-concentration transplantation group(group C), a low-concentration transplantation group(group D) and a placebo transplantation group(group E). The expression of nerve growth factor(NGF)protein in the retinal layers was detected by immunohistochemical staining at 2, 4, 6 and ***: The expression of NGF was positive in group A and most positive in the retinal ganglion cell layer. In groups B and E, the expression of NGF was positive 2wk after transplantation and showed an increase in all layers. However, the level of expression had decreased in all layers at 4wk and was significantly reduced at 8wk. In groups C and D, the expression of NGF had increased at 2wk and continued to increase up to 8wk. The level of expression in group C was much higher than that in group ***: DR can be improved by intravitreal injection of human umbilical mesenchymal stem *** concentrations of human umbilical mesenchymal stem cells confer a better protective effect on DR than low concentrations.

关键词： diabetic retinopathy human umbilical mesenchymal stem cells nerve growth factor stem cell therapy

Differentiation of isolated human umbilical cord mesenchymal stem cells into neural stem cells

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International Journal of Ophthalmology(English edition) 2016年第1期9卷 41-47页

作者： Song Chen Wei Zhang Ji-Ming wang Hong-Tao Duan Jia-Hui Kong yue-xin wang Meng Dong Xue Bi Jian Song Tianjin Eye Hospital Tianjin Key Lab of Ophthalmology and Visual Science Tianjin Eye Institute Clinical College of Ophthalmology Tianjin Medical University

AIM：To investigate whether umbilical cord human mesenchymal stem cell（UC-MSC）was able to differentiate into neural stem cell and neuron.·METHODS：The umbilical cords were o btained from pregnant women with their written consent and the approval of the Clinic Ethnics ***-MSC were isolated by adherent culture in the medium contains 20%fetal bovine serum（FBS）,then they were maintained in the medium contain 10%FBS and induced to neural cells in neural differentiation *** investigated whether UC-MSC was able to differentiate into neural stem cell and neuron by using flow cytometry,reverse transcriptase-polymerase chain reaction（RT-PCR）and immunofluorescence（IF）analyzes.·R ESULTS：A substantial number of UC-MSC was harvested using the tissue explants adherent method at about *** cytometric study revealed that these cells expressed common markers of MSCs,such as CD105（SH2）,CD73（SH3）and *** induction of differentiation of neural stem cells,the cells began to form clusters;RT-PCR and IF showed that the neuron specific enolase（NSE）and neurogenic differentiation 1-positive cells reached 87.3%±14.7%and 72.6%±11.8%,*** showed neuronal cell differentiation after induced,including neuron-like protrusions,plump cell body,obviously and stronger ***-PCR and IF analysis showed that microtubule-associated protein 2（MAP2）and nuclear factor-M-positive cells reached 43.1%±10.3%and 69.4%±19.5%,respectively.·CONCLUSION：Human umbilical cord derived MSCs can be cultured and proliferated and differentiate into neural stem cells,which may be a valuable source for cell therapy of neurodegenerative eye diseases.

关键词： human umbilical cord mesenchymal stemcells neural stem cells neuron neurodegenerative eye diseases

RIG-I,a novel DAMPs sensor for myoglobin,activates NF-κB/caspase-3 signaling in CS-AKI model

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Military Medical Research 2022年第1期9卷 40-52页

作者： Peng-Tao wang Ning Li xin-yue wang Jia-Le Chen Chen-Hao Geng Zi-Quan Liu Hao-Jun Fan Qi Lv Shi-Ke Hou Yan-Hua Gong General Hospital of Tianjin Medical University Tianjin 300052China institute of Disaster Medicine Tianjin UniversityTianjin 300072China State Key Laboratory of Medicinal Chemical Biology Nankai UniversityTianjin 300350China Tianjin Key Laboratory of Disaster Medicine Technology Tianjin 300072China Wenzhou Safety(Emergency)Institute Tianjin UniversityWenzhou 325000Zhejiang China.

Background:Acute kidney injury(AKI)is the main life-threatening complication of crush syndrome(CS),and myoglobin is accepted as the main pathogenic *** pattern recognition receptor retinoicacid-inducible gene I(RIG-I)has been reported to exert anti-viral effects function in the innate immune ***,it is not clear whether RIG-I plays a role in *** present research was carried out to explore the role of RIG-I in ***:Sprague-Dawley rats were randomly divided into two groups:the sham and CS groups(n=12).After administration of anesthesia,the double hind limbs of rats in the CS group were put under a pressure of 3 kg for 16 h to mimic crush *** rats in both groups were denied access to food and *** were sacrificed at 12 h or 36 h after pressure was *** successful establishment of the CS-AKI model was confirmed by serum biochemical analysis and renal histological *** addition,RNA sequencing was performed on rat kidney tissue to identify molecular pathways involved in ***,NRK-52 E cells were treated with 200μmol/L ferrous myoglobin to mimic CS-AKI at the cellular *** cells and cell supernatant samples were collected at 6 h or 24 *** interfering RNAs(siRNA)was used to knock down RIG-I *** relative expression levels of molecules involved in the RIG-I pathway in rat kidney or cells samples were measured by quantitative real-time PCR(qPCR),Western blotting analysis,and immunohistochemistry(IHC)*** necrosis factor-α(TNF-α)was d etected by ***-immunoprecipitation(Co-IP)assays were used to detect the interaction between RIG-I and ***:RNA sequencing of CS-AKI rat kidney tissue revealed that the different expression of RIG-I signaling ***,Western blotting,and IHC assays showed that RIG-I,nuclear factor kappa-B(NF-κB)P65,p-P65,and the a poptotic marker caspase-3 and cleaved caspase-3 were up-regulated in the CS group(P<0.05).However,the

关键词： Crush syndrome Acute kidney injury Retinoic acid-inducible gene I Myoglobin Nuclear factor kappa-B/caspase-3 Damage-associated molecular patterns

Glytan decreases portal pressure via mesentery vasoconstriction in portal hypertensive rats

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World Journal of Gastroenterology 2014年第44期20卷 16674-16682页

作者： Qing-Hong Du Lin Han Jun-Jie Jiang Ya Xu Wei-Hong Li Peng-Tao Li xin-yue wang Xu Jia School of Fundamental Medicine Beijing University of Chinese Medicine Institute of Basic Research in Clinical Medicine China Academy of Chinese Medical Sciences Dongzhimen Hospital Affiliated to Beijing University of Chinese Medicine

AIM: To investigate the effects of Glytan on splanchnic hemodynamics and its reduction of portal pressure in portal hypertensive rats.

关键词： Glytan Portal hypertension Hemodynamics Mesentery Endothelin-1 Receptor Sensitivity

Paclitaxel-etoposide-carboplatin/cisplatin versus etoposidecarboplatin/cisplatin as first-line treatment for combined small-cell lung cancer: a retrospective analysis of 62 cases

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Cancer Biology & Medicine 2015年第2期12卷 117-125页

作者： yue-Ya Li Chan Zhou Deng-Xia Yang Jing wang Zhu-Jun Liu xin-yue wang Kai Li Department of Thoracic Oncology Tianjin Medical University Cancer Institute and Hospital National Clinical Research Center for Cancer Key Laboratory of Cancer Prevention and Therapy Tianjin Lung Cancer Diagnosis and Treatment Center

Objective: To compare the efficacy and adverse effects of paclitaxel-etoposide-carboplatin/cisplatin(TEP/TCE) regimen with those of etoposide-carboplatin/cisplatin(EP/CE) regimen as first-line treatment for combined small-cell lung cancer(CSCLC).Methods: A retrospective study was conducted on 62 CSCLC patients who were treated at Tianjin Medical University Cancer Institute and Hospital from July 2000 to April 2013 and administered with TEP/TCE regimen(n=19) or EP/CE regimen(n=43) as first-line CSCLC treatment. All patients received more than two cycles of chemotherapy, and the response was evaluated every two cycles. The primary endpoint was overall survival(OS), and the secondary endpoints were progression-free survival(PFS), objective response rate(ORR), disease control rate(DCR), and adverse effects. Results: ORR between the TEP/TCE and EP/CE groups showed a statistical difference(90% vs. 53%, P=0.033). Both groups failed to reach a statistical difference in DCR(100% vs. 86%, P=0.212). The median PFS and OS of the TEP/TCE group were slightly longer than those of the EP/CE group, although both groups failed to reach a statistical difference(10.5 vs. 8.9 months, P=0.484; 24.0 vs. 17.5 months, P=0.457). However, stratified analysis indicated that the PFS of patients with stages III and IV CSCLC showed marginally significant difference between the TEP/TCE and EP/CE groups(19.5 vs. 7.6 months; P=0.071). Both rates of grade IV bone marrow depression and termination of chemotherapy in the TEP/TCE group were significantly higher than those in the EP/CE group(26.3% vs. 7.0%, P=0.036; 31.6% vs. 14.7%, P=0.004). Conclusion: The TEP/TCE regimen may not be preferred for CSCLC, and this three-drug regimen requires further exploration and research. To date, the EP/CE regimen remains the standard treatment for CSCLC patients.

关键词： Small cell lung carcinoma (SCLC) chemotherapy CE regimen adverse effects survival analysis prognosis

Utilising network pharmacology and molecular dockingtoexplore the mechanism of Sangbaipi Decoction in the treatment of acute exacerbation of chronic obstructive pulmonary disease

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TMR Aging 2021年第2期3卷 9-18页

作者： yue-Jing Liu Yi-Hua Fan Li Ma Tang-Wei Feng xin-yue wang Rui wang xin-Ju Li Tianjin University of Traditional Chinese Medicine Tianjin 301617China First Teaching Hospital of Tianjin University of Traditional Chinese Medicine 300193Tianjin

Objective To explore themechanism of Sangbaipi Decoction(SBPD)in the treatment of acute exacer-bation of chronic obstructive pulmonary disease(AECOPD).Methods The active compounds of SBPD and targets of those active compounds were collectedfrom the TCMSP *** we built the AECOPD target database by OMIM,GeneCards,PharmGKB and *** intersectional targets arethep-otentialtargets of SBPD in the treatment *** built"Potential Active Compounds-Drug-AECOPDTargets"Network via Cytoscape *** the Protein-Protein Inter-action(PPI)network through STRING *** analyze the PPI network and“Potential Active Compounds-Drug-AECOPD Targets”Network via CytoNCA,then we got the core targets and key active compounds of SBPDin the treatment ***(GO)function enrichment and KEGG pathway enrichment on the intersection targets were analyzed by R *** key active compounds is molecularly docked with the core target protein receptors through AutoDock Vina soft-ware,and the 2D ligand-protein interaction diagramsare drawn through LigPlot 2.2 *** were 109 active compounds,205 targets of SBPD.2837 targetsrelated to AECOPD were picked out.157 intersectional targets were obtained from the two *** get 3 coretargets(TP53,JUN,VEGFA)and five key active compounds(quercetin,luteolin,kaempferol,wogonin,arachidonic acid)of *** GO function enrichment analysis showed that 2552 entries(P<0.05),of which there were 2261 biological processes(BP)items,and 84 related items of cell composition(CC),and 207 molecular function(MF)*** pathway enrichment analysis showed that 167 signaling path-ways(P<0.05),mainly including IL 17 signaling pathway,TNF signaling pathway,HIF-1 signaling *** molecular dock-ing structure shows that the key active compounds of SBPD have good affinities with the core *** may treatAECOPD by anti-inflammatory,anti-oxidation,airway mucus secretion reduction,and pulm

关键词： Sangbaipi Decoction network pharmacology mechanism molecular docking acute exacerbation of chronic obstructive pulmonary disease