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GATA3 siRNA inhibits the binding of NFAT1 to interleukin-13 promoter in human T cells

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Chinese Medical Journal 2010年第6期123卷 739-744页

作者： YAO xin yang yan HE Hai-yan WANG Min YIN Kai-sheng HUANG Mao Department of Respiratory Disease First Affiliated Hospital of Nanjing Medical UniversityNanjingJiangsu 210029China

Background Intedeukin-13 （IL-13） is recognized to be a key modulator in the pathogenesis of Th2-induced allergic inflammation. Transcription factors GATA3 and NFAT1 have been both implicated in the regulation of Th2 cytokines. We previously demonstrated the GATA3-NFAT1 association during human T cell activation. However, the function of the GATA3-NFAT1 complex in Th2 cytokines regulation is still unknown. Small interference RNA （siRNA） was constructed to knock down GATA3 expression in Hut-78 cells to investigate the possible role of GATA3-NFAT1 complex in IL-13 *** Cells were stimulated with anti-CD3 plus anti-CD28 antibodies to mimic in vivo antigen-mediated co-stimulation; the expression of IL-13 mRNA was determined by real-time PCR; chromation immunoprecipitation （CHIP） assay was employed to investigate the NFAT1 binding to IL-13 promoter. Results GATA3 siRNA suppressed the expression of GATA3 both in mRNA and protein levels in Hut-78 cells. The binding of NFAT1 to IL-13 promoter was inhibited by GATA3 siRNA in activated T cells, which was followed by the reduction of IL-13 *** GATA3-NFAT1 complex may play an important role in the regulation of IL-13 transcription in human T cells.

关键词： interleukin-13 asthma GATA3 NFAT1

Synthesis and Luminescence Properties of New Red Phosphor BaZnBO3F：Eu3＋ for Light-Emitting Diode

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材料科学研究（中英文版） 2014年第3期3卷 52-56页

作者： Wanping Chen Xiaoyan Dai xin yang yan Liu Huaihua Key Laboratory of Functional Inorganic & Polymeric Materials Department of Chemistry and Chemical EngineeringHuaihua University HuaiHua 418008 P. R. China

LINE-1 family member GCRG123 gene is up-regulated in human gastric signet-ring cell carcinoma

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World Journal of Gastroenterology 2008年第5期14卷 758-763页

作者： Gang-Shi Wang Meng-Wei Wang Ben-yan Wu xin-yan yang Wei-Hua Wang Wei-Di You Department of Gerontal Gastroenterology General Hospital of Chinese PLA Beijing 100853 China

AIM： To analyze the expression profiles of a human gastriccancer-related gene, GCRG123, in human gastric signetring cell carcinoma tissues, and to perform bioinformatics analysis on GCRG123.METHODS： In situ hybridization was used to explore the GCRG123 expression pattern in paraffin-embedded gastric tissues, including 15 cases of signet-ring cell carcinoma, 15 of intestinal-type adenocarcinoma, and 15 of normal gastric mucosa. Northern blotting was used to analyze the differences in GCRG123 expression between stomach signet-ring cell carcinoma and intestinal-type adenocarcinoma tissues. Online software, including BLAST, Multalin and BLAT, were applied for bioinformatics analysis. National Center for Biotechnology Information （NCBI） and the University of California Santa Cruz （UCSC） databases were used for the analyses.RESULTS： The in situ hybridization signal appeared as blue precipitates restricted to the cytoplasm. Ten out of 15 cases of gastric signet ring cell carcinoma, normal gastric mucosal epithelium and pyloric glands showed high GCRG123 expression. Low GCRG123 expression was observed in gastric intestinal-type adenocarcinoma and normal gastric glands. Northern blotting revealed that GCRG123 was up-regulated in signet-ring cell carcinoma tissue but down-regulated in intestinal-type adenocarcinoma tissue. BLAST and Multalin analyses revealed that the GCRG123 sequence had 92% similarity with the ORF2 sequence of human long interspersed nuclear element retrotransposons （LINE-1, L1）. BLAT analysis indicated that GCRG123 mapped to all chromosomes. GCRG123 was found to integrate in the intron-17 and -23 of Rb, 5＇ flanking region of IL-2 and clotting factor Ⅸ genes.CONCLUSION： GCRG123, an active member of the Lt family, was up-regulated in human gastric signet-ring cell carcinoma.

关键词： Stomach Signet-ring cell carcinoma GCRG123 LINE-1 In situ hybridization Bioinformatics

Inhibition of interleukin-13 gene expression in T cells through GATA-3 pathway by arsenic trioxide

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Chinese Medical Journal 2008年第22期121卷 2346-2349页

作者： YAO xin HE Hai-yan yang yan DAI Shan-lin SUN Pei-li YIN Kai-sheng HUANG Mao Department of Respiratory Diseases the First Affiliated Hospital of Nanjing Medical University Nanjing Jiangsu 210029 China

Aarsenic trioxide （AT） has a long history of use in both traditional Chinese medicine and in modem medicine in asthma therapy. Recently, Yin et al＇ found that AT even at small doses reduced the airway inflammation of sensitized guinea pigs. However the mechanism underlying this is still largely unknown. Interleukin 13 （IL-13）, as one of the important TH2 cytokines, plays an important role in asthma pathogenesis through promoting eosinophilic inflammation,

关键词： interleukin-13 asthma GATA-3 arsenic trioxide

Targeting TrkB–PSD-95 coupling to mitigate neurological disorders

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Neural Regeneration Research 2025年第3期20卷 715-724页

作者： xin yang Yu-Wen Alvin Huang John Marshall Department of Molecular Biology Cell Biology and BiochemistryBrown UniversityProvidenceRIUSA Department of Neurology Warren Alpert Medical School of Brown UniversityProvidenceRIUSA Center for Translational Neuroscience Robert J.and Nancy D.Carney Institute for Brain Science and Brown Institute for Translational ScienceBrown UniversityProvidenceRIUSA

Tropomyosin receptor kinase B(TrkB)signaling plays a pivotal role in dendritic growth and dendritic spine formation to promote learning and *** activity-dependent release of brain-derived neurotrophic factor at synapses binds to pre-or postsynaptic TrkB resulting in the strengthening of synapses,reflected by long-term ***,the association of postsynaptic density protein-95 with TrkB enhances phospholipase Cγ-Ca^(2+)/calmodulin-dependent protein kinaseⅡand phosphatidylinositol 3-kinase-mechanistic target of rapamycin signaling required for long-term *** this review,we discuss TrkB-postsynaptic density protein-95 coupling as a promising strategy to magnify brain-derived neurotrophic factor signaling towards the development of novel therapeutics for specific neurological disorders.A reduction of TrkB signaling has been observed in neurodegenerative disorders,such as Alzheimer's disease and Huntington's disease,and enhancement of postsynaptic density protein-95 association with TrkB signaling could mitigate the observed deficiency of neuronal connectivity in schizophrenia and *** with brain-derived neurotrophic factor is problematic,due to poor pharmacokinetics,low brain penetration,and side effects resulting from activation of the p75 neurotrophin receptor or the truncated TrkB.T1 *** TrkB agonists and antibodies that activate TrkB are being intensively investigated,they cannot distinguish the multiple human TrkB splicing isoforms or cell type-specific *** TrkB–postsynaptic density protein-95 coupling provides an alternative approach to specifically boost TrkB signaling at localized synaptic sites versus global stimulation that risks many adverse side effects.

关键词： Angelman syndrome autism brain-derived neurotrophic factor depression neurodegenerative disorder neurodevelopmental disorder postsynaptic density protein-95 synaptic plasticity TrkB

Defect Engineering:Can it Mitigate Strong Coulomb Effect of Mg^(2+)in Cathode Materials for Rechargeable Magnesium Batteries?

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Nano-Micro Letters 2025年第1期17卷 135-159页

作者： Zhengqing Fan Ruimin Li xin Zhang Wanyu Zhao Zhenghui Pan Xiaowei yang School of Chemistry and Chemical Engineering Shanghai Jiao Tong UniversityShanghai 200240People’s Republic of China School of Chemistry and Chemical Engineering Taiyuan University of TechnologyTaiyuan 030024People’s Republic of China School of Materials Science and Engineering Tongji UniversityShanghai 201804People’s Republic of China

Rechargeable magnesium batteries(RMBs)have been considered a promising“post lithium-ion battery”system to meet the rapidly increasing demand of the emerging electric vehicle and grid energy storage ***,the sluggish diffusion kinetics of bivalent Mg^(2+)in the host material,related to the strong Coulomb effect between Mg^(2+)and host anion lattices,hinders their further development toward practical *** engineering,regarded as an effective strategy to break through the slow migration puzzle,has been validated in various cathode materials for *** this review,we first thoroughly understand the intrinsic mechanism of Mg^(2+)diffusion in cathode materials,from which the key factors affecting ion diffusion are further ***,the positive effects of purposely introduced defects,including vacancy and doping,and the corresponding strategies for introducing various defects are *** applications of defect engineering in cathode materials for RMBs with advanced electrochemical properties are also ***,the existing challenges and future perspectives of defect engineering in cathode materials for the overall high-performance RMBs are described.

关键词： Rechargeable magnesium battery Sluggish diffusion kinetic Defect engineering Cathode materials Ion migration

A novel gene,GCRG224,is differentially expressed in human gastric mucosa

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World Journal of Gastroenterology 2003年第1期9卷 30-34页

作者： Gang-Shi Wang Meng-Wei Wang Ben-yan Wu Wei-Di You xin-yan yang Department of Gastroenterology,General Hospital of Chinese PLA,Beijing 100853,China Department of Gastroenterology General Hospital of Chinese PLA Beijing 100853 China. wanggangshi@***

AIM: To clone genes that may predispose us to humangastric cancer and to analyze it's expression in gastric ***: Specimens of paired tumor, paratumor andnormal gastric mucosa tissues collected from fifteen patientswho suffered from stomach antrum adenocarcinoma wereused for analysis. Seven out of the fifteen cases were firststudied by fluorescent differential display reversetranscription polymerase chain reaction (DDTR-PCR) *** differentially expressed bands of interest were cloned,analyzed by Northern blot, sequencing and RT-PCR. ThroughBLAST, the sequencing results were compared with GenBankdatabase for homology analysis. In situ hybridization withDIG-labeled cRNA probes was used to analyze the expressionof interesting cDNA bands in paraffin embedded pairednormal gastric mucosa and cancer tissues isolated from 30gastric adenocarcinoma ***: DDRT-PCR showed that one of the interestingcDNA bands, which was named W2, expressed much higherin all seven tested tumor and paratumor samples than intheir normal counterparts, it was sub-cloned into a pGEM-TEasy vector. Two subclones were subsequently *** of the subclone, GCRG224, was studied further. Thesequencing result showed that GCRG224 consisted of 1 159base pairs and had one open reading frame (ORF). It locatedat human chromosome 11q14. No homologue was found inGenBank database with GCRG224-ORF. This nucleotidesequence data were submitted to GenBank with accessionNo. AF438406. RT-PCR showed that GCRG224 expressedhigher in 11/15 gastric cancer tissues than in non-tumortissues. However, the result of Northern blot analysis showeda higher GCRG224 expression in the non-tumor tissue thanin the tumor one. Human multiple tissue Northern blot analysisrevealed that GCRG224 also expressed in human normal colontissue, and peripheral blood leukocyte. In situ hybridizationanalysis showed that only 5/30 adenocarcinoma, 3/18dysplasia and 6/18 intestinal metaplasia showed higherGCRG224 express

关键词： GCRG224基因胃癌胃粘膜聚合酶链反应克隆肿瘤病理学

A Study on the Synthesis of Biphenyl Bis（imidazoline） Ligands

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A Study on the Synthesis of Biphenyl Bis（imidazoline） Liga...

Revealing and predicting the long-term biomechanical response of orthokeratology by developing a patient-specific computational model

第十七届全国金属有机化学学术讨论会

作者： Wei Pang xin-yan yang Biao Gao Zhao Wang xin-Qi Hao Xue Mei Zhao 宋毛平 College of Chemistry and Molecular Engineering Henan Key Laboratory of Chemical Biology and Organic ChemistryZhengzhou University

＜正＞The preparation and application of the biphenyl bis（oxazaoline）ligands have made great progress[1-4].Since biphenyl bis（imidazolin）e ligands have very similar structure with biphenyl bis（oxazoline）ligands,we try to synthesize the biphenyl bis（imidazoline）ligands and study their ***,we have obtained products a,b and c by using 2,2’-biphenyldicarboxylic acid as the starting material.

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Science China(Physics,Mechanics & Astronomy) 2025年第1期Mechanics & Astronomy) .卷 156-168页

作者： Yifeng Li Zhuoran yang Ziming yan Huibin Shi Zhanli Liu Kaijie Wang Department of Engineering Mechanics School of Aerospace Engineering Tsinghua University Beijing Tongren Eye Center Beijing Tongren Hospital Capital Medical University Beijing Ophthalmology & Visual Sciences Key Lab

Orthokeratology(OK) is widely used for effective myopia correction and control. However, the incomplete understanding of its biomechanical mechanisms makes OK lens fitting rely heavily on clinician judgment, complicating accurate predictions of treatment outcomes. In this paper, we performed clinical experiments and numerical analysis to study corneal deformation modes and long-term changes in central corneal thickness. Clinical experiments were conducted on 194 Chinese myopic patients under OK treatment for 3 months. Based on the experimental data, a patient-specific computational biomechanical model for OK was established and validated. Specifically, the anisotropic mechanical properties of the cornea were incorporated into the model to describe the significant difference between its shear modulus(29.5 kPa) and tensile modulus(768.4 kPa). Additionally, a viscohyperelastic material model with a prolonged corneal relaxation time of 5.6 h was developed to capture the long-term deformation response. The results show that corneal thickness reduction in OK is primarily due to out-of-plane shear deformation,influenced by the cornea's low shear resistance. Modeling the extended corneal relaxation time is crucial for predicting long-term biomechanical responses. The computational model effectively captures long-term changes in central corneal thickness, potentially improving OK lens fitting accuracy.

关键词： orthokeratology computational biomechanical model long-term viscoelasticity myopia correction