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Neuroscience Bulletin 2018年第6期34卷 1007-1016页

作者： Yan-Yan Chang xin-wei gong Hai-Qing gong Pei-Ji Liang Pu-Ming Zhang Qin-Chi Lu Department of Neurology Ren Ji Hospital School of Medicine Shanghai Jiao Tong University School of Biomedical Engineering Shanghai Jiao Tong University

Exploring the transition from inter-ictal to ictal epileptiform discharges(IDs) and how GABAAreceptormediated action affects the onset of IDs will enrich our understanding of epileptogenesis and epilepsy *** used Mg2+-free artificial cerebrospinal fluid(ACSF) to induce epileptiform discharges in juvenile mouse hippocampal slices and used a micro-electrode array to record the discharges. After the slices were exposed to Mg2+-free ACSF for 10 min–20 min, synchronous recurrent seizurelike events were recorded across the slices, and each event evolved from inter-ictal epileptiform discharges(IIDs) to pre-ictal epileptiform discharges(PIDs), and then to *** the transition from IIDs to PIDs, the duration of discharges increased and the inter-discharge interval decreased. After adding 3 lmol/L of the GABAAreceptor agonist muscimol, PIDs and IDs disappeared, and IIDs remained. Further, the application of 10 lmol/L muscimol abolished all the epileptiform discharges. When the GABAAreceptor antagonist bicuculline was applied at 10 lmol/L, IIDs and PIDs disappeared, and IDs remained at decreased intervals. These results indicated that there are dynamic changes in the hippocampal network preceding the onset of IDs, and GABAAreceptor activity suppresses the transition from IIDs to IDs in juvenile mouse hippocampus.

关键词： Epileptiform discharge Gamma-aminobutyric acid Bicuculline Muscimol Micro-electrode array Hippocampal slice

Pharmacokinetics and the bystander effect in CD：：UPRT/5-FC bi-gene therapy of glioma

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Chinese Medical Journal 2009年第11期122卷 1267-1272页

作者： SHI De-zhi HU wei-xing LI Li-xin CHEN gong wei Dong GU Pei-yuan Department of Neurosurgery First Affiliated Hospital of Nanjing Medical University Nanjing Jiangsu 210029 China

Background Cytosine deaminase （CD） converts 5-fluorocytosine （5-FC） to 5-fluorouracil （5-FU） in CD/5-FC gene therapy, 5-FU will be mostly converted into nontoxic 13-alanine without uracil phosphoribosyltransferase （UPRT）. UPRT catalyzes the conversion of 5-FU to 5-fluorouridine monophosphate, which directly kills CD：：UPRT-expressing cells and surrounding cells via the bystander effect. But the pharmacokinetics and the bystander effect of CD：：UPRT/5-FC has not been verified in vivo and in vitro. Before the CD：：UPRT/5-FC bi-gene therapy system is used in clinical trial, it is essential to monitor the transgene expression and function in vivo. Thus, we developed a preclinical tumor model to investigate the feasibility of using ^19F-magnetic resonance spectroscopy （^19F-MRS） and optical imaging to measure non-invasive CD and UPRT expression and its bystander effect. Methods C6 and C6-CD：：UPRT cells were cultured with 5-FC. The medium, cells and their mixture were analyzed by ^19F-MRS. Rats with intracranial xenografted encephalic C6-CD：：UPRT glioma were injected intraperitoneally with 5-FC and their ^19F-MRS spectra recorded. Then the pharmacokinetics of 5-FC was proved. Mixtures of C6 and C6-CD：：UPRT cells at different ratios were cultured with 5-FC and the cytotoxic efficacy and survival rate of cells recorded. To determine the mechanism of the bystander effect, the culture media from cell comprising 25% and 75% C6-CD：：UPRT cells were examined by ^19F-MRS. A comparative study of mean was performed using analysis of variance （ANOVA）. Results ^19F-MRS on samples from C6-CD：：UPRT cells cultured with 5-FC showed three broad resonance signals corresponding to 5-FC, 5-FU and fluorinated nucleotides （F-Nuctd）. For the C6 mixture, only the 5-FC peak was detected. In vivo serial ^19F-MRS spectra showed a strong 5-FC peak and a weak 5-FU peak at 20 minutes after 5-FC injection. The 5-FU concentration reached a maximum at about 50 minutes. The F-Nuctd signal appeared after about

关键词： glioma cytosine deaminase uracil phosphoribosyltransferase pharmacokinetics magnetic resonance spectroscopy bystander effect

Spatiotemporal dynamics of high-K^+-induced epileptiform discharges in hippocampal slice and the effects of valproate

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Neuroscience Bulletin 2013年第1期29卷 28-36页

作者： Jian-Sheng Liu Jing-Bo Li xin-wei gong Hai-Qing gong Pu-Ming Zhang Pei-Ji Liang Qin-Chi Lu Department of Neurology Renji Hospital School of Medicine Shanghai Jiao Tong University Department of Biomedical Engineering Shanghai Jiao Tong University

The epileptic seizure is a dynamic process involving a rapid transition from normal activity to a state of hypersynchronous neuronal discharges. Here we investigated the network properties of epileptiform discharges in hippocampal slices in the presence of high K ＋ concentration （8.5 mmol/L） in the bath, and the effects of the anti-epileptic drug valproate （VPA） on epileptiform discharges, using a microelectrode array. We demonstrated that epileptiform discharges were predominantly initiated from the stratum pyramidale layer of CA3a-b and propagated bi-directionally to CA1 and CA3c. Disconnection of CA3 from CA1 abolished the discharges in CA1 without disrupting the initiation of discharges in CA3. Further pharmacological experiments showed that VPA at a clinically relevant concentration （100 μmol/L） suppressed the propagation speed but not the rate or duration of high-K＋-induced discharges. Our findings suggest that pacemakers exist in the CA3a-b region for the generation of epileptiform discharges in the hippocampus. VPA reduces the conduction of such discharges in the network by reducing the propagation speed.

关键词： epileptiform discharges hippocampal slices microelectrode array valproate

浅谈大学英语课程中跨文化素质的培养——以电子科技大学成都学院为例

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科教导刊 2018年第3期 25-26页

作者：胡莘龚伟电子科技大学成都学院四川成都610000

语言是文化最重要的载体,语言又受文化的影响,文化对语言有制约作用。语言与文化密不可分,只有学会了语言所承载的文化知识,学习者才能真正地去理解和掌握一门语言,从而运用语言进行有效、成功的交流。本文提出了教师在传授英语语言技... 详细信息

语言是文化最重要的载体,语言又受文化的影响,文化对语言有制约作用。语言与文化密不可分,只有学会了语言所承载的文化知识,学习者才能真正地去理解和掌握一门语言,从而运用语言进行有效、成功的交流。本文提出了教师在传授英语语言技能的同时应该加强英语国家文化背景知识的讲授,以及如何提高学生的语言理解能力和跨文化交际能力。

关键词：语言文化英语教学交际能力跨文化

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三溴甲烷的毒性作用概述

中国工业医学杂志 2016年第5期29卷 356-358页

作者：丁恩民刘炘龚伟朱宝立南京医科大学公共卫生学院职业医学与环境卫生学系江苏南京211166 江苏省疾病预防控制中心职业病防治所江苏南京210009

三溴甲烷在生活、生产上有着广泛的用途,主要用作溶剂、致冷剂、防火化学品以及有机合成的中间体和药物制造。进入环境中的三溴甲烷大多是用氯气给水消毒时产生的副产物,而企业在生产和使用三溴甲烷及贮运过程中,作业工人会因接触三溴... 详细信息

三溴甲烷在生活、生产上有着广泛的用途,主要用作溶剂、致冷剂、防火化学品以及有机合成的中间体和药物制造。进入环境中的三溴甲烷大多是用氯气给水消毒时产生的副产物,而企业在生产和使用三溴甲烷及贮运过程中,作业工人会因接触三溴甲烷而产生健康损害。由于三溴甲烷环境和职业暴露途径广泛,其毒性和健康风险也越来越受到人们的关注,需要尽快制定和完善三溴甲烷的职业和环境接触限值。

关键词：三溴甲烷毒性

Potassium and calcium channels in different nerve cells act as therapeutic targets in neurological disorders

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Neural Regeneration Research 2025年第5期20卷 1258-1276页

作者： Qing Qiu Mengting Yang Danfeng gong Haiying Liang Tingting Chen Department of Pharmacology School of PharmacyNantong UniversityNantongJiangsu ProvinceChina Jiangsu Province Key Laboratory of Inflammation and Molecular Drug Target NantongJiangsu ProvinceChina Department of Pharmacy Longyan First Affiliated Hospital of Fujian Medical UniversityLongyanFujian ProvinceChina

The central nervous system, information integration center of the body, is mainly composed of neurons and glial cells. The neuron is one of the most basic and important structural and functional units of the central nervous system, with sensory stimulation and excitation conduction functions. Astrocytes and microglia belong to the glial cell family, which is the main source of cytokines and represents the main defense system of the central nervous system. Nerve cells undergo neurotransmission or gliotransmission, which regulates neuronal activity via the ion channels, receptors, or transporters expressed on nerve cell membranes. Ion channels, composed of large transmembrane proteins, play crucial roles in maintaining nerve cell homeostasis. These channels are also important for control of the membrane potential and in the secretion of neurotransmitters. A variety of cellular functions and life activities, including functional regulation of the central nervous system, the generation and conduction of nerve excitation, the occurrence of receptor potential, heart pulsation, smooth muscle peristalsis, skeletal muscle contraction, and hormone secretion, are closely related to ion channels associated with passive transmembrane transport. Two types of ion channels in the central nervous system, potassium channels and calcium channels, are closely related to various neurological disorders, including Alzheimer's disease, Parkinson's disease, and epilepsy. Accordingly, various drugs that can affect these ion channels have been explored deeply to provide new directions for the treatment of these neurological disorders. In this review, we focus on the functions of potassium and calcium ion channels in different nerve cells and their involvement in neurological disorders such as Parkinson's disease, Alzheimer's disease, depression, epilepsy, autism, and rare disorders. We also describe several clinical drugs that target potassium or calcium channels in nerve cells and could be u

关键词： astrocytes calcium channels central nervous system extracellular ion concentration microglia neurological disorders neurons potassium channels

The Superlinear Convergence Analysis of a Nonmonotone BFGS Algorithm on Convex Objective Functions

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Acta Mathematica Sinica,English Series 2008年第1期24卷 35-42页

作者： gong Lin YUAN Zeng xin wei School of Science East China University of Science and Technology Shanghai 200237 P. R. China College of Mathematics and Information Science Guangxi University Nanning 530004 P. R. China

We prove the superlinear convergence of a nonmonotone BFGS algorithm on convex objective functions under suitable conditions.

关键词： BFGS method superlinear convergence nonmonotone linesearch

Application type identification of Internet flows based on medium mathematics

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The Journal of China Universities of Posts and Telecommunications 2010年第6期17卷 72-79,88页

作者： CHENG wei-qing gong Jian DING wei SUN Zhi-xin Computer College Nanjing University of Posts and Telecommunications Nanjing 210003 China School of Computer Science and Technology Southeast University Nanjing 210096 China State Key Laboratory of Software Development Environment Beijing 100191 China

Internet application identification is needed by network management in many aspects,such as quality of service （QoS） management,intrusion detection,traffic engineering,accounting,and so on. This article makes an in-depth study of precise identification of Internet applications by using flow characteristics instead of well-known port or application signature match. A novel approach that identifies the application type of an Internet protocol （IP） flow by finding what flow the flow looks the most like based on medium mathematics system （MMS） is proposed. The approach differs from previous ones mainly in two aspects：it has inherent scalability due to its use of the measure of n-dimensional medium truth degree; not only features of a flow,but also the association between the flow and the other flows of the same host as well as the relation among all flows of a host are employed to recognize a flow＇s application type. For the present,some popular applications are concentrated on,and up to six application types can be identified with better accuracy. The results of experiments conducted on Internet show that the proposed methodology is effective and deserves attention.

关键词： application type identification flow characteristics measure of medium truth degree MMS

Experimental and simulated characterization of creep behavior of P92 steel with prior cyclic loading damage

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Journal of Materials Science & Technology 2017年第12期33卷 1540-1548页

作者： wei Zhang Xiaowei Wang Jianming gong Yong Jiang xin Huang School of Mechanical and Power Engineering Nanjing Tech UniversityNanjing 211816China Jiangsu Key Lab of Design and Manufacture of Extreme Pressure Equipment Nanjing 211816China Department of Material Textiles and Chemical EngineeringFaculty of Engineering and ArchitectureGhent University.Zwijnaarde B-9052Belgium

The effect of prior cyclic loading on creep behavior of P92 steel was investigated. Creep tests on prior cyclic loading exposure specimens were performed at 650？C and 130 MPa. In order to clarify the influence of prior cyclic loading on creep behavior, optical microscope, scanning electron microscope and transmission electron microscope were used. Experimental results indicate that the prior cyclic loading degrades the creep strength significantly. However, the degradation tends to be saturated with further increase in prior cyclic loading. From the view of microstructural evolution, the recovery of martensite laths takes place during prior cyclic loading exposure. This facilitates the dislocation movement during the following creep process. Therefore, premature rupture of creep test occurs. Additionally, saturated behavior of degradation can be attributed to the near completed recovery of martensite laths. Based on the effect of prior cyclic loading, a newly modified Hayhurst creep damage model was proposed to consider the prior cyclic loading damage. The main advantage of the proposed model lies in its ability to directly predict creep behavior with different levels of prior cyclic loading damage. Comparison of the predicted and experimental results shows that the proposed model can give a reasonable prediction for creep behavior of P92 steel with different level of prior cyclic loading damage.

关键词： Prior cyclic loading Creep behavior Martensite morphology Life prediction

Angular Momentum Josephson Effect between Two Isolated Condensates

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Chinese Physics Letters 2021年第6期38卷 1-6页

作者： wei-Feng Zhuang Yue-xin Huang Ming gong CAS Key Laboratory of Quantum Information University of Science and Technology of ChinaHefei 230026China Synergetic Innovation Center of Quantum Information and Quantum Physics University of Science and Technology of ChinaHefei 230026China

We demonstrate that the two degenerate energy levels in spin–orbit coupled trapped Bose gases,coupled by a quenched Zeeman field,can be used for angular momentum Josephson *** a static quenched field,we can realize a Josephson oscillation with a period ranging from millisecond to hundreds of ***,by a driven Zeeman field,we realize a new Josephson oscillation,in which the population imbalance may have the same expression as the current in the direct-current Josephson *** the dynamics of the condensate cannot follow up the modulation frequency,it is in the self-trapping *** new dynamic is understood from the time-dependent evolution of the constant-energy trajectory in the phase *** model has several salient advantages compared to the previous *** condensates are isolated from their excitations by a finite gap,thus can greatly suppress the damping effect induced by thermal atoms and Bogoliubov *** oscillation period can be tuned by several orders of magnitude without influencing other *** experiments,the dynamics can be mapped out from spin and momentum spaces,thus it is not limited by the spatial resolution in absorption *** system can serve as a promising platform for matter wave interferometry and quantum metrology.

关键词： Josephson oscillation momentum