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Food Bioscience 2022年第4期48卷 398-405页

作者： Gui-Li Huang Tian-Tian Liu Jia-Jia Ma Ling-Xiang Sun Si-yao Sui xin-yao quan Yu-Ning Wang Agricultural Product Storage and Processing Laboratory Suzhou Academy of Agricultural SciencesSuzhou215105China Key Laboratory of Carbohydrate Chemistry and Biotechnology Science Center for Future FoodsJiangnan UniversityWuxi214122China Jiangsu Key Laboratory for Biomass Energy and Material Institute of Chemical Industry of Forest Products CAFNanjing210042China

The harvested loquat fruits are prone to browning,leading to a decline in fruit *** activity of polyphenol oxidase(PPO)is an important way to prevent *** this study,we evaluated the inhibitory impact of oligomeric procyanidins(OPC)on hydroxylation of monophenol and oxidation of o-diphenol catalyzed by PPO and effect of OPC on loquat fruits *** obtained results revealed that OPC could attenuate the activity of monophenolase and *** monophenolase activity,the inhibition rate was observed to be dose-dependent with an IC50 of 11.6±3.4μg/mL for the steady-state *** diphenolase activity,OPC inhibited PPO activity(IC50=15.75±2.1μg/mL)with a reversible and competitive mechanism and quenched fluorescence of PPO by forming OPC-PPO complex with one binding site in a static *** addition,the data obtained from storage assays revealed that OPC could enhance the quality of harvested loquat fruits by attenuating the PPO activity,augmenting the content of total phenolics and flavonoids,and simultaneously delaying the browning of *** this research,we identified a potent PPO inhibitor and expanded its potential as a food preservative.

关键词： Polyphenol oxidase Oligomeric procyanidins Inhibitory mechanism Fluorescence quenching Storage

Chimeric molecules facilitate the degradation of androgen receptors and repress the growth of LNCaP cells

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Asian Journal of Andrology 2009年第1期11卷 119-126页

作者： Yue-Qing Tang Bang-Min Han xin-quan yao Yan Hong Yan Wang Fu-Jun Zhao Sheng-Qiang Yu Xiao-Wen Sun Shu-Jie Xia Department of Urology Shanghai First People＇s Hospital Institute of Urology Department of Urology Wujiang Third People＇s Hospital Suzhou 215228 China Department of Immunology Shanghai Jiao Tong University Shanghai 200025 China

Post-translational degradation of protein plays an important role in cell life. We employed chimeric molecules （dihydrotestosterone-based proteolysis-targeting chimeric molecule [DHT-PROTAC]） to facilitate androgen receptor （AR） degradation via the ubiquitin-proteasome pathway （UPP） and to investigate the role of AR in cell proliferation and viability in androgen-sensitive prostate cancer cells. Western blot analysis and immunohistochemistry were applied to analyse AR levels in LNCaP cells after DHT-PROTAC treatment. Cell counting and the 3-（4,5-dimethylthiazol-2-yl）-2,5- diphenyl tetrazolium bromide （MTT） cell viability assay were used to evaluate cell proliferation and viability after AR elimination in both LNCaP and PC-3 cells. AR was tagged for elimination via the UPP by DHT-PROTAC, and this could be blocked by proteasome inhibitors. Degradation of AR depended on DHT-PROTAC concentration, and either DHT or an ALAPYIP-（arg）8 peptide could compete with DHT-PROTAC. Inhibition of cell proliferation and decreased viability were observed in LNCaP cells, but not in PC-3 or 786-0 cells after DHT-PROTAC treatment. These data indicate that AR elimination is facilitated via the UPP by DHT-PROTAC, and that the growth of LNCaP cells is repressed after AR degradation.

关键词： androgen receptor LNCaP prostate cancer proteolysis ubiquitin

采后流通温度对精品包装苏州青感官及内在品质的影响

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江苏农业学报 2023年第1期39卷 218-228页

作者：马佳佳孙灵湘黄桂丽全鑫瑶隋思瑶王毓宁江苏太湖地区农业科学研究所江苏苏州215105

为进一步明确采后流通影响精品包装叶菜品质的温度和关键环节,以苏州青为试验对象,模拟产地处理→贮藏/流通→配送末端→货架的流通程序,研究苏州青在流通过程中呼吸代谢、外观、质地、风味、滋味及营养物质含量的变化。结果表明,与常温... 详细信息

为进一步明确采后流通影响精品包装叶菜品质的温度和关键环节,以苏州青为试验对象,模拟产地处理→贮藏/流通→配送末端→货架的流通程序,研究苏州青在流通过程中呼吸代谢、外观、质地、风味、滋味及营养物质含量的变化。结果表明,与常温(20.0℃)流通相比,低温(5.0℃)流通抑制了苏州青的总呼吸速率与糖酵解途径(Embden-Meyerhof-Parnas,EMP)、三羧酸循环(Tricarboxylic acid cycle,TCA)途径的呼吸速率,保持了其苦味、涩味,并具有较高的甜味氨基酸含量增幅,延缓了其组织剪切力、咸味的上升及叶绿素含量的损失。综合分析可知,EMP途径是苏州青模拟流通条件下的呼吸主路径,各呼吸代谢路径对流通温度存在差异化响应,货架1 d是总呼吸以及EMP、TCA途径呼吸的转折点,流通贮藏是影响苏州青剪切力和整体挥发性气味的重要环节。真空预冷环节可以延缓苏州青质地变化,也会造成叶绿素a损失。研究结果可为精品包装苏州青的流通温度控制提供理论依据和数据支撑。

关键词：温度流通环节苏州青品质

MOFs的合成及其在食品包装中的应用研究进展

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包装工程 2022年第21期43卷 78-88页

作者：全鑫瑶隋思瑶黄桂丽孙灵湘马佳佳王毓宁苏州市农业科学院江苏苏州215105

目的通过对金属-有机框架材料(Metal Organic Frames,MOFs)的合成方法及其在食品包装中的研究进展进行简要介绍和分类总结,为MOFs基功能性食品包装材料的设计和制备提供新的见解,并推动MOFs在食品包装领域中的实际应用。方法介绍了MOFs... 详细信息

目的通过对金属-有机框架材料(Metal Organic Frames,MOFs)的合成方法及其在食品包装中的研究进展进行简要介绍和分类总结,为MOFs基功能性食品包装材料的设计和制备提供新的见解,并推动MOFs在食品包装领域中的实际应用。方法介绍了MOFs材料的合成机理,概述了共沉淀法、扩散法、溶剂热法、微波合成法等MOFs材料的制备方法,根据功能分类介绍了MOFs及其复合材料在抗菌、保鲜、催熟、检测等食品包装领域的研究进展,并讨论了MOFs在食品包装应用中的挑战和机遇。结论可以对MOFs的组成和结构进行设计和调整,从而获得丰富的物化特性,以实现食品包装所需的特定功能。MOFs材料在未来功能化、智能化食品包装领域中具有良好的应用价值和产业化前景。

关键词：金属-有机框架纳米材料食品包装保鲜

不同提取方法对马蹄皮总提取物抗氧化活性的影响

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食品研究与开发 2017年第8期38卷 22-26页

作者：陈秋娟谢微罗杨合李金凤王胜新全耀贺州学院化学与生物工程学院广西贺州542899 贺州学院食品科学与工程技术研究院广西贺州542899

利用不同提取方法提取获得马蹄皮总提取物,研究不同提取物的总还原能力和羟自由基清除能力。结果显示:不同提取物均具有不同程度的总还原能力和羟自由基清除能力,且均具有量效关系,表明不同提取物均具有一定的抗氧化能力;其中,抗氧能力... 详细信息

利用不同提取方法提取获得马蹄皮总提取物,研究不同提取物的总还原能力和羟自由基清除能力。结果显示:不同提取物均具有不同程度的总还原能力和羟自由基清除能力,且均具有量效关系,表明不同提取物均具有一定的抗氧化能力;其中,抗氧能力最强的是用酸性乙醇作提取剂微波辅助提取的提取物。

关键词：马蹄皮总还原能力羟自由基抗氧化

Experimental and numerical study on protective effect of RC blast wall against air shock wave

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Defence Technology（防务技术） 2024年第1期31卷 567-579页

作者： xin-zhe Nian quan-min Xie xin-li Kong Ying-kang yao Kui Huang State Key Laboratory of Precision Blasting Jianghan UniversityWuhan430056China Hubei Key Laboratory of Blasting Engineering Jianghan UniversityWuhan430056China Hubei(Wuhan)Institute of Explosion and Blasting Technology Jianghan UniversityWuhan430056China College of Defense Engineering Army Engineering University of PLANanjing210007China Institute of Defense Engineering AMSPLALuoyang471023China

Prototype experiments were carried out on the explosion-proof performance of the RC blast *** mass of TNT detonated in the experiments is 5 kg and 20 kg *** shock wave overpressure was tested in different *** above experiments were numerically simulated,and the simulated shock wave overpressure waveforms were compared with that tested and given by CONWEP *** results show that the numerically simulated waveform is slightly different from the test waveform,but similar to CONWEP *** dimensional analysis and numerical simulation under different working conditions,the equation for the attenuation rate of the diffraction overpressure behind the blast wall was *** to the corresponding standards,the degree of casualties and the damage degree of the brick concrete building at a certain distance behind the wall can be determined when parameters are *** above results can provide a reference for the design and construction of the reinforced concrete blast wall.

关键词： Blast wall Shock wave Diffraction Overpressure Protection

Ultrabroad Terahertz Bandpass Filter Based on a Multiple-Layered Metamaterial with Flexible Substrates

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Chinese Physics Letters 2012年第9期29卷 84-86页

作者： LIANG Lan-Ju yao Jian-quan YAN xin College of Opto-electronics Engineering Zaozhuang UniversityZaozhuang 277160 College of Precision Instrument and Opto-electronics Engineering Institute of Laser and Opto-electronicsTianjin UniversityTianjin 300072 Key Laboratory of Opto-electronics Information Technology(Ministry of Education) Tianjin UniversityTianjin 300072

An ultrabroad and sharp transition bandpass flexible terahertz(THz)filter is designed using a multiple-layered *** bandpass filter has excellent filtering capability,with a 3 dB bandwidth of about 0.47 THz and sharp band-edge transitions of 80 dB/THz and 96 dB/THz,respectively,and it can be realized by a coupling individual resonance *** find that the geometry parameters have an influence on the transmission profile,which are capable of giving us meaningful guidance in design of high profile bandpass THz *** numerical results show that the multiple-layered flexible metamaterial provides an effective way to achieve ultrabroad THz devices.

关键词： sharp layered meaningful

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青稞分蘖角度的QTL定位

作物学报 2025年第1期51卷 260-272页

作者：杨景发余鑫莲姚有华姚晓华王蕾吴昆仑李新青海大学农林科学院/青海省作物分子育种重点实验室/青海省青稞遗传育种重点实验室/青藏高原种质资源研究与利用实验室/青海大学省部共建三江源生态与高原农牧业国家重点实验室青海西宁810016

分蘖角度是青稞株型的主要构成因素,在提高青稞产量与抗倒伏性中发挥着极为重要的作用。为解析青稞分蘖角度的遗传机制,本研究以青稞品种‘达章紫’(株型松散)和‘昆仑10号’(株型紧凑)为亲本构建的RIL群体为材料,基于高密度遗传图谱,... 详细信息

分蘖角度是青稞株型的主要构成因素,在提高青稞产量与抗倒伏性中发挥着极为重要的作用。为解析青稞分蘖角度的遗传机制,本研究以青稞品种‘达章紫’(株型松散)和‘昆仑10号’(株型紧凑)为亲本构建的RIL群体为材料,基于高密度遗传图谱,在多环境中进行青稞分蘖角度的QTL定位,并利用RIL群体衍生的剩余杂合系,对鉴定到的主效QTLqTA7H-1进行精细定位。在青稞7条染色体上共检测到9个控制分蘖角度的QTL,解释表型变异为6.41%~33.57%,其中,qTA3H-1和qTA7H-1为多环境共检测的主效QTL,平均加性效应分别为5.42°(增效)和-3.87°(减效);在减效QTL qTA7H-1初定位区间筛选到4个剩余杂合体,自交衍生F8:9近等基因系,在置信区间内加密设计14对分子标记对RHL群体极端单株进行检测,利用获得的5种交换类型单株,将qTA7H-1进一步界定在PC08(32,252,397)与PA10(41,790,765)约9.54Mb的物理区间内。本研究初步揭示调控青稞分蘖角度的遗传因子,为开展分蘖角度性状的遗传改良与分子设计育种奠定了基础。

关键词：青稞分蘖角度 QTL定位主效位点

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Enantioselective reductive conjugate alkenylation of α,β-enones with keto alkenyl acetates by nickel catalysis

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Science China Chemistry 2025年第1期 157-162页

作者： Rong-De He Yun-Lei Luo Qiu-quan Pan Qi-Wei yao xing-Zhong Shu State Key Laboratory of Applied Organic Chemistry College of Chemistry and Chemical Engineering Lanzhou University

Reactions involving alkenyl acetates offer cost-effective and environmentally friendly routes for alkene synthesis, yet their asymmetric variant remains elusive. Concurrently, asymmetric conjugate alkenylation predominantly centered on nucleophilic addition using alkenyl–M. This manuscript presents an asymmetric reductive conjugate alkenylation reaction involving alkenyl acetates. The method facilitates the enantioselective addition of keto alkenyl groups to α,β-enones, resulting in the formation of unsaturated diketones—a class of useful structural motifs that are challenging to access otherwise. The use of electron-rich Pyroxy ligand is essential for achieving both high reaction efficiency and enantioselectivity.

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Broadband Terahertz Transmission Modulation Based on Hybrid Graphene-Metal Metamaterial

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Journal of Electronic Science and Technology 2018年第2期16卷 98-104页

作者： Lan-Ju Liang Zhang Zhang xin Yan xin Ding De-quan Wei Qi-Li Yang Zhen-Hua Li Jian-quan yao College of Precision Instrument and Opto-Electronics Engineering Tianjin UniversityTianjin 300072 School of Opto-Electronic Engineering Zaozhuang UniversityZaozhuang 277160

A novel patterned metamaterial,composed of graphene layer and metal periodic array of split ring resonators （SRRs） and cross-shapedresonators （CSRs）, with broadband terahertz （THz） wave modulation was proposed and theoretically studied. It demonstrated that a broad passband high transmission of over 96.1% in the frequency range from 1.02 THz to 1.66 THz and two narrow band resonance frequencies f1 and f2 could be generated. The modulation depth of transmission was 29.2% when the graphene layer was covered on the metal metamaterial surface, and the modulation depth could be further increased by increasing the Fermi energy of graphene layer and reached approximately 79.5% at 1.0 eV in a broadband THz frequency range. The resonance frequencies of f1 and f2 were blue-shifted, and their modulation depths reached about 63.2% and 18%, respectively. These results show that the ultrathin graphene-metal metamaterial exhibits potential to achieve highperformance active THz devices and may offer widespread applications.

关键词： Graphene metamaterial modulator terahertz