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New insights into the heat responses of grape leaves via combined phosphoproteomic and acetylproteomic analyses

Horticulture Research 2019年第1期6卷 489-503页

作者： Guo-Tian liu Jian-Fu Jiang xin-na liu Jin-Zhu Jiang Lei Sun Wei Duan Rui-Min Li Yi Wang David Lecourieux Chong-Huai liu Shao-Hua Li Li-Jun Wang Beijing Key Laboratory of Grape Science and Enology and Key Laboratory of Plant Resources Institute of Botanythe Chinese Academy of SciencesBeijing 100093China Zhengzhou Fruit Research Institute Chinese Academy of Agricultural SciencesZhengzhou 450009China College of Horticulture Northwest A&F UniversityYangling 712100China Universite´de Bordeaux ISVVEcophysiologie et Ge´nomique Fonctionnelle de la VigneUMR 1287F-33140 Villenave d’OrnonFrance INRA ISVVEcophysiologie et Ge´nomique Fonctionnelle de la VigneUMR 1287F-33140 Villenave d’OrnonFrance University of Chinese Academy of Sciences Beijing 100093China

Heat stress is a serious and widespread threat to the quality and yield of many crop species,including grape(Vitis vinifera L.),which is cultivated ***,we conducted phosphoproteomic and acetylproteomic analyses of leaves of grape plants cultivated under four distinct temperature *** phosphorylation or acetylation of a total of 1011 phosphoproteins with 1828 phosphosites and 96 acetyl proteins with 148 acetyl sites changed when plants were grown at 35℃,40℃,and 45℃in comparison with the proteome profiles of plants grown at 25℃.The greatest number of changes was observed at the relatively high *** classification and enrichment analysis indicated that phosphorylation,rather than acetylation,of serine/arginine-rich splicing factors was involved in the response to high *** finding is congruent with previous observations by which alternative splicing events occurred more frequently in grapevine under high *** in acetylation patterns were more common than changes in phosphorylation patterns in photosynthesis-related proteins at high temperatures,while heat-shock proteins were associated more with modifications involving phosphorylation than with those involving *** proteins were identified with changes associated with both phosphorylation and acetylation,which is consistent with crosstalk between these posttranslational modification types.

关键词： temperature cultivated Vitis

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Comparison of the major malformation rate of children conceived from cryopreserved embryos and fresh embryos

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Chinese Medical Journal 2010年第14期123卷 1893-1897页

作者： LI Hong-zhen QIAO Jie CHI Hong-bin CHEN xin-na liu Ping MA Cai-hong Medical Center -for Human Reproduction Department of Obstetrics and Gynecology Peking University Third Hospital Beijing 100191 China

Background Cryopreserved embryo transfer has become indispensable in reproductive technology. More and more children are conceived from frozen-thawed embryo transfer （FET）. The risk of birth defects associated with frozen-thawed embryo transfer has been evaluated and conflict results are obtained. The aim of this study was to compare the rate of major malformations in children conceived from cryopreserved embryos with that of children from fresh embryos. Methods A retrospective analysis was performed on children conceived from frozen-thawed embryos and fresh embryos between January 2005 and December 2008 at the Reproduction Center of the Third Hospital, Peking University. The major malformation rates were compared between two groups for all children, as welt as singletons or twins, separately. The frequencies of different subtypes of malformations classified according to different organ system were also compared. Results Thirty-four of 3125 children from cryopreserved embryos had a major malformation. The malformation rate was 1.09%, which was comparable to that for children after fresh embryos transfer （1.53%（55/3604）, OR： 0.71, 95%Cl; 0.46-1.09）. The malformation rate was also similar when the analysis was limited to children from cryopreserved embryos resulted from in vitro fertilization （IVF）（1.39%）and fresh IVF（1.3%）. However, children from cryopreserved embryos resulted from intracytoplasmic sperm injections （ICSI） had much lower malformation rate than from fresh ICS1（0.63% vs.1.83%, OR： 0.34, 95% CI： 0.16-0.75）. No difference was found in the incidence of major malformations in singletons from cryo ICSI （0.73%） and fresh ICS1（1.9%）, or from cryo IVF（1.49%） and fresh IVF（1.67%）. Similar malformation rate was found in multiples from cryo ICS1（0.52%） and fresh ICS1（1.76%）, or cryo IVF（1.30%） and fresh IVF（0.90%）. The distribution and risk of the subtype of malformations, such as cardiovascular, gastrointestinal, neural tube, urogenital, musculoskeletal and facia

关键词： congenital malformation fertilization in vitro sperm injections, intracytoplasmic frozen-thawed embryo transfer

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Maximizing biomass utilization: An integrated strategy for coproducing multiple chemicals

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Journal of Energy Chemistry 2025年第1期100卷 180-191页

作者： Byeongchan Ahn Sua Jo Jonggeol na J.Jay liu Young-Ju Kim Wangyun Won Department of Chemical and Biological Engineering Korea University Department of Chemical Engineering (Integrated process) Kyung Hee University Department of Chemical Engineering and Materials Science Ewha Womans University Department of Chemical Engineering Pukyong National University Resources Engineering Plant Research Department Korea Institute of Geoscience and Mineral Resources

Lignocellulosic biomass is one of the viable solutions to alleviate the global warming. However, the limited utilization of biomass majorly focused on cellulose and hemicellulose restricts the economic and environmental feasibilities. To cope with this issue, we proposed an integrated process of co-producing 1,6-hexanediol（1,6-HDO） with tetrahydrofuran and adipic acid from biomass, referred to as Strategy A. To compare the impacts of lignin upgrading and feedstock, Strategy B, which co-produces tetrahydrofuran alone, and Strategy C, which is the traditional route to produce 1,6-HDO from fossil fuels, were used. Heat networks are also designed to reduce operating costs and indirect carbon emissions due to energy consumption, saving 87% and 83% of the heat and cooling requirements, respectively, in Strategy A. The market competitiveness of Strategy A was evaluated by determining the minimum selling price through techno-economic analysis, and sustainability was thoroughly investigated by quantifying the environmental impacts through both midpoint and endpoint life-cycle assessments（LCAs）.Strategy A was found to be the most favorable both economically（USRDSCHARDOLLAR3,402/ton） and environmentally（-26.9 kg CO2eq.）. This indicates that lignin valorization is not only economically but also environmentally preferred. Finally, changes in economic and environmental feasibilities depending on economic, process, and environmental parameters were investigated using sensitivity and uncertainty analyses. The results of these analyses provide valuable insight into bio-based chemical production.

关键词： Biorefinery Carbon mitigation Climate change Global warming Lignin utilization Bioeconomy

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碱金属修饰g-C_3N_4的能带结构调控与载流子迁移过程

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物理化学学报 2016年第10期32卷 2488-2494页

作者：祝林马新国刘娜徐国旺黄楚云湖北工业大学理学院武汉430068 湖北工业大学太阳能高效湖北省协同创新中心武汉430068

采用平面波超软赝势方法研究了Li、na、K三种碱金属离子修饰对g-C_3N_4能带结构和载流子迁移过程的影响。对建立的六种吸附构型分别采用广义梯度近似和局域密度近似进行计算,发现三种碱金属离子均更趋向于吸附在g-C_3N_4片层内的大空洞... 详细信息

采用平面波超软赝势方法研究了Li、na、K三种碱金属离子修饰对g-C_3N_4能带结构和载流子迁移过程的影响。对建立的六种吸附构型分别采用广义梯度近似和局域密度近似进行计算,发现三种碱金属离子均更趋向于吸附在g-C_3N_4片层内的大空洞中央位置(F位置)。对于碱金属与g-C_3N_4形成的n型Schottky结,通过能带结构和功函数的计算,发现界面电荷的平衡使g-C_3N_4的能带电位分别整体下移1.52V(Li)、1.07V(na)、0.86V(K)。其中K离子的引入一方面将g-C_3N_4的价带和导带调整到更合适的氧化还原电位,另一方面增大了g-C_3N_4的最高占据轨道(HOMO)和最低未占据轨道(LOMO)的分布,有利于提高载流子的迁移率,同时出现的HOMO和LOMO轨道非共面特性有利于电子和空穴的分离。

关键词：光催化 g-C3N4 能带结构载流子迁移

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枫香叶片衰老过程中光合能力的变化

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应用生态学报 2016年第10期27卷 3129-3136页

作者：罗紫东关华德章新平刘娜湖南师范大学资源与环境科学学院长沙410081 福林德斯大学环境学院

利用Li-6400XT便携式光合作用测定系统,于2014年10—12月测定枫香叶片衰老过程中光合作用光响应曲线,采用叶氏模型和非直角双曲线模型进行模拟,分析枫香叶片衰老过程中光合能力的变化.结果表明:随着枫香叶片逐渐变黄变红,其净光合速率... 详细信息

利用Li-6400XT便携式光合作用测定系统,于2014年10—12月测定枫香叶片衰老过程中光合作用光响应曲线,采用叶氏模型和非直角双曲线模型进行模拟,分析枫香叶片衰老过程中光合能力的变化.结果表明:随着枫香叶片逐渐变黄变红,其净光合速率的光响应能力逐渐降低,实测的最大净光合速率从叶片开始泛黄时的2.88μmol CO_2·m^(-2)·s^(-1)下降到叶片衰老后期(12月8日)的0.95μmol CO_2·m^(-2)·s^(-1).2种光响应模型均较好地模拟了观测的光响应数据,其中叶氏模型表现更优.模拟得到的最大净光合速率、表观量子效率、光补偿点的量子效率、暗呼吸速率等参数均随枫香叶片衰老凋落而逐渐下降,反映出枫香叶片衰老过程中光合能力缓慢下降的过程.在树梢红叶未落期间,枫香叶片仍具有一定的净光合作用能力,这有利于增加秋冬季节的碳吸收量.

关键词：光合能力光响应模型光合作用模拟叶片衰老

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Research status and prospects of the fractal analysis of metal material surfaces and interfaces

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International Journal of Minerals,Metallurgy and Materials 2025年第1期32卷 20-38页

作者： Qinjin Dai Xuefeng liu xin Ma Shaojie Tian Qinghe Cui Beijing Advanced Innovation Center for Materials Genome Engineering University of Science and Technology BeijingBeijing 100083China Beijing Laboratory of Metallic Materials and Processing for Modern Transportation University of Science and Technology BeijingBeijing 100083China Laboratory for Advanced Materials Processing of Ministry of Education University of Science and Technology BeijingBeijing 100083China School of Mechanical and Electrical Engineering Xinjiang Institute of EngineeringUrumqi 830023China

As a mathematical analysis method,fractal analysis can be used to quantitatively describe irregular shapes with self-similar or self-affine *** analysis has been used to characterize the shapes of metal materials at various scales and *** methods make it difficult to quantitatively describe the relationship between the regular characteristics and properties of metal material surfaces and ***,fractal analysis can be used to quantitatively describe the shape characteristics of metal materials and to establish the quantitative relationships between the shape characteristics and various properties of metal *** the perspective of two-dimensional planes and three-dimensional curved surfaces,this paper reviews the current research status of the fractal analysis of metal precipitate interfaces,metal grain boundary interfaces,metal-deposited film surfaces,metal fracture surfaces,metal machined surfaces,and metal wear *** relationship between the fractal dimensions and properties of metal material surfaces and interfaces is *** from three perspectives of fractal analysis,namely,research scope,image acquisition methods,and calculation methods,this paper identifies the direction of research on fractal analysis of metal material surfaces and interfaces that need to be *** is believed that revealing the deep influence mechanism between the fractal dimensions and properties of metal material surfaces and interfaces will be the key research direction of the fractal analysis of metal materials in the future.

关键词： metal material surfaces and interfaces fractal analysis fractal dimension homogeneity

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Zwitterionic hyaluronic acid derivatives for co-delivery of both chemotherapeutic and nucleic acid drugs in breast cancer treatment

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nano Research 2025年第1期18卷 607-617页

作者： Ruo-Lin Jiang Hui-na liu Yu-Fan Yang Zhi-Cheng Zhang Qi Dai Xiao-Yan Bao Lin-Jie Wu Ya-xin Qin xin Tan Xiao-Yan Sun Xu-Fang Ying Zhi-Qing Ben Min Han Institute of Pharmaceutics College of Pharmaceutical SciencesZhejiang UniversityHangzhou 310058China Department of Radiation Oncology Key Laboratory of Cancer Prevention and InterventionThe Second Affiliated HospitalCollege of MedicineZhejiang UniversityHangzhou 310058China Hangzhou Institute of Innovative Medicine Zhejiang UniversityHangzhou 310058China Jinhua Institute of Zhejiang University Jinhua 321299China national Key Laboratory of Advanced Drug Delivery and Release Systems Zhejiang UniversityHangzhou 310058China Polytechnic institute Zhejiang UniversityHangzhou 310015China

Traditionally,hyaluronic acid has been widely used for drug delivery,but the current application bottleneck is that hyaluronic acid is hydrophilic and electronegative,which makes it difficult to carry hydrophobic drugs and small interfering Rna(siRna)with the same *** on previous studies,we designed and synthesized hyaluronic acid nanocarriers HA-spermine/N,N,N-trimethylcystamine/DOX-TPP(HSTD)for loading siRna to overcome the problem of siRna release caused by strong electrostatic ***,N,N,Ntrimethylcystamine in the carrier can be degraded by intracellular glutathione to completely and rapidly release siRna,thus promoting ***,when codelivered with the chemotherapy drug doxorubicin(DOX),this novel nanocarrier showed promising synergy in inhibiting tumor growth.

关键词： zwitterionic hyaluronic acid N N N-trimethylcystamine small interfering Rna(siRna) doxorubicin

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Comparative proteomic analysis of plasma exosomes reveals the functional contribution of N-acetyl-alpha-glucosaminidase to Parkinson’s disease

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Neural Regeneration Research 2025年第10期20卷 2998-3012页

作者： Yuan Zhao Yidan Zhang xin liu Jian Zhang Ya Gao Shuyue Li Cui Chang Xiang liu Guofeng Yang Department of Geriatrics Second Hospital of Hebei Medical UniversityShijiazhuangHebei ProvinceChina Department of Neurosurgery The Second Hospital of Hebei Medical UniversityShijiazhuangHebei ProvinceChina

Parkinson’s disease is the second most common progressive neurodegenerative disorder,and few reliable biomarkers are available to track disease *** proteins,Dna,mRna,and lipids carried by exosomes reflect intracellular changes,and thus can serve as biomarkers for a variety of *** this study,we investigated alterations in the protein content of plasma exosomes derived from patients with Parkinson’s disease and the potential therapeutic roles of these proteins in Parkinson’s *** a tandem mass tag-based quantitative proteomics approach,we characterized the proteomes of plasma exosomes derived from individual patients,identified exosomal protein signatures specific to patients with Parkinson’s disease,and identified N-acetyl-alpha-glucosaminidase as a differentially expressed protein.N-acetyl-alpha-glucosaminidase expression levels in exosomes from the plasma of patients and healthy controls were validated by enzyme-linked immunosorbent assay and western *** results demonstrated that the exosomal N-acetyl-alpha-glucosaminidase concentration was not only lower in Parkinson’s disease,but also decreased with increasing Hoehn-Yahr stage,suggesting that N-acetyl-alpha-glucosaminidase could be used to rapidly evaluate Parkinson’s disease ***,western blot and immunohistochemistry analysis showed that N-acetyl-alpha-glucosaminidase levels were markedly reduced both in cells treated with 1-methyl-4-phenylpyridinium and cells overexpressingα-synuclein compared with control ***,N-acetyl-alpha-glucosaminidase overexpression significantly increased cell viability and inhibitedα-synuclein expression in 1-methyl-4-phenylpyridinium-treated *** together,our findings demonstrate for the first time that exosomal N-acetyl-alpha-glucosaminidase may serve as a biomarker for Parkinson’s disease diagnosis,and that N-acetyl-alpha-glucosaminidase may reduceα-synuclein expression and 1-methyl-4-phenylpyridinium-induced n

关键词： biomarker diagnosis exosomes N-acetyl-alpha-glucosaminidase Parkinson’s disease proteomic α-synuclein

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个体化方案在胃癌营养支持患者中的应用效果

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癌症进展 2017年第2期15卷 189-192页

作者：陈昕刘娜邱辉刘明哈尔滨医科大学附属第一医院内分泌科哈尔滨1500010

目的探讨个体化护理方案在胃癌营养支持患者中的应用效果。方法选取实施个体化护理模式的胃癌患者55例为观察组,选取实施常规护理模式的胃癌患者55例为对照组。术前及营养支持结束后测量患者的体重、血清白蛋白(ALB)、前白蛋白(PAB)和... 详细信息

目的探讨个体化护理方案在胃癌营养支持患者中的应用效果。方法选取实施个体化护理模式的胃癌患者55例为观察组,选取实施常规护理模式的胃癌患者55例为对照组。术前及营养支持结束后测量患者的体重、血清白蛋白(ALB)、前白蛋白(PAB)和转铁蛋白(TRF)等营养指标及免疫指标(T细胞亚群CD4^+、CD8^+、CD4^+/CD8^+和IgG、IgA、Ig M)。实施个体化护理前后,对护理质量和患者满意度进行调查统计。结果营养支持后,两组患者的ALB、TRF和PAB水平均较本组营养支持前升高(P﹤0.05),两组间各指标水平比较,差异无统计学意义(P﹥0.05);营养支持后,两组患者的IgG、IgA、Ig M、CD4^+、CD8^+和CD4^+/CD8^+水平均较本组营养支持前升高(P﹤0.05),两组间各指标水平比较,差异无统计学意义(P﹥0.05);观察组患者对护理人员技术水平、服务态度、仪表仪容、住院环境、医护措施和巡视病情及应急处理的满意度均高于对照组患者(P﹤0.05);个体化护理方案实施后,观察组护理人员病室管理、护理书写、重患者护理、急救管理、消毒隔离和药品管理评分均较本组护理前高,差异均有统计学意义(P﹤0.05),观察组各指标评分均高于对照组,差异有统计学意义(P﹤0.05)。结论个体化护理模式在胃癌患者营养支持中的应用,主要效果体现在提高了环境整洁度,提高了护理工作效率,培养了良好的工作作风,提高了优质服务,加强和深化了安全预警意识,提高了患者对护理的满意度,值得临床推广。

关键词：个体化护理胃癌营养支持常规护理

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Single-cell omics: experimental workflow, data analyses and applications

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Science China Life Sciences 2025年第1期68卷 5-102页

作者： Fengying Sun Haoyan Li Dongqing Sun Shaliu Fu Lei Gu xin Shao Qinqin Wang xin Dong Bin Duan Feiyang xing Jun Wu Minmin Xiao Fangqing Zhao Jing-Dong J.Han Qi liu Xiaohui Fan Chen Li Chenfei Wang Tieliu Shi Department of Clinical Laboratory the Affiliated Wuhu Hospital of East China Normal University (The Second People's Hospital of Wuhu City) Pharmaceutical Informatics Institute College of Pharmaceutical SciencesZhejiang University Key Laboratory of Spine and Spinal Cord Injury Repair and Regeneration (Tongji University) Ministry of EducationOrthopaedic DepartmentTongji HospitalBioinformatics DepartmentSchool of Life Sciences and TechnologyTongji University Frontier Science Center for Stem Cells School of Life Sciences and TechnologyTongji University Translational Medical Center for Stem Cell Therapy and Institute for Regenerative Medicine Shanghai East HospitalBioinformatics DepartmentSchool of Life Sciences and TechnologyTongji University Research Institute of Intelligent Computing Zhejiang Lab Shanghai Research Institute for Intelligent Autonomous Systems Center for Single-cell Omics School of Public HealthShanghai Jiao Tong University School of Medicine national Key Laboratory of Chinese Medicine Modernization Innovation Center of Yangtze River DeltaZhejiang University Center for Bioinformatics and Computational Biology Shanghai Key Laboratory of Regulatory Biologythe Institute of Biomedical Sciences and School of Life SciencesEast China Normal University Beijing Institutes of Life Science Chinese Academy of Sciences Peking-Tsinghua Center for Life Sciences Academy for Advanced Interdisciplinary StudiesCenter for Quantitative Biology (CQB)Peking University Zhejiang Key Laboratory of Precision Diagnosis and Therapy for Major Gynecological Diseases Women's HospitalZhejiang University School of Medicine Key Laboratory of Advanced Theory and Application in Statistics and Data Science-MOE School of StatisticsEast China Normal University

Cells are the fundamental units of biological systems and exhibit unique development trajectories and molecular features. Our exploration of how the genomes orchestrate the formation and maintenance of each cell, and control the cellular phenotypes of various organismsis, is both captivating and intricate. Since the inception of the first single-cell Rna technology, technologies related to single-cell sequencing have experienced rapid advancements in recent years. These technologies have expanded horizontally to include single-cell genome, epigenome,proteome, and metabolome, while vertically, they have progressed to integrate multiple omics data and incorporate additional information such as spatial sc Rna-seq and CRISPR screening. Single-cell omics represent a groundbreaking advancement in the biomedical field,offering profound insights into the understanding of complex diseases, including cancers. Here, we comprehensively summarize recent advances in single-cell omics technologies, with a specific focus on the methodology section. This overview aims to guide researchers in selecting appropriate methods for single-cell sequencing and related data analysis.

关键词： single-cell sequencing genome epigenome proteomics metabolomics multimodal spatial transcriptomics CRISPR screening

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