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6-gingerol protects nucleus pulposus-derived mesenchymal stem cells from oxidative injury by activating autophagy

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World Journal of Stem Cells 2020年第12期12卷 1603-1622页

作者： Li-Ping Nan feng Wang Yang Liu Zhong Wu xin-min feng Jun-Jian Liu Liang Zhang Department of Orthopedic Tongji University School of MedicineShanghai Tenth People’s HospitalTenth People’s Hospital of Tongji UniversityShanghai 200072China Department of Spine Surgery Tongji University School of MedicineShanghai East HospitalShanghai 200120China Department of Orthopedic West China Hospital of Sichuan UniversityChengdu 610000Sichuan ProvinceChina Department of Orthopedics Shanghai Tenth People’s HospitalTongji University School of MedicineShanghai 200072China Department of Orthopedics Clinical Medical College of Yangzhou UniversityYangzhou 225000Jiangsu ProvinceChina

BACKGROUND To date,there has been no effective treatment for intervertebral disc degeneration(IDD).Nucleus pulposus-derived mesenchymal stem cells(NPMSCs)showed encouraging results in IDD treatment,but the overexpression of reactive oxygen species(ROS)impaired the endogenous repair abilities of NPMSCs.6-gingerol(6-GIN)is an antioxidant and anti-inflammatory reagent that might protect NPMSCs from *** To investigate the effect of 6-GIN on NPMSCs under oxidative conditions and the potential *** The cholecystokinin-8 assay was used to evaluate the cytotoxicity of hydrogen peroxide and the protective effects of *** levels were measured by 2´7´-dichlorofluorescin diacetate *** metalloproteinase(MMP)was detected by the tetraethylbenzimidazolylcarbocyanine iodide *** assay and Annexin V/PI double-staining were used to determine the apoptosis ***,autophagy-related proteins(Beclin-1,LC-3,and p62),apoptosisassociated proteins(Bcl-2,Bax,and caspase-3),and PI3K/Akt signaling pathwayrelated proteins(PI3K and Akt)were evaluated by Western blot *** were detected by transmission electron microscopy in ***-3 was also detected by *** mRNA expression of collagen II and aggrecan was evaluated by real-time polymerase chain reaction(RT-PCR),and the changes in collagen II and MMP-13 expression were verified through an immunofluorescence *** 6-GIN exhibited protective effects against hydrogen peroxide-induced injury in NPMSCs,decreased hydrogen peroxide-induced intracellular ROS levels,and inhibited cell apoptosis.6-GIN could increase Bcl-2 expression and decrease Bax and caspase-3 *** MMP,Annexin V-FITC/PI flow cytometry and TUNEL assay results further confirmed that 6-GIN treatment significantly inhibited NPMSC apoptosis induced by hydrogen peroxide.6-GIN treatment promoted extracellular matrix(ECM)expression by reducing the oxidative stress injury-induced increas

关键词： Nucleus pulposus-derived mesenchymal stem cells 6-Gingerol Intervertebral disc degeneration Oxidative stress Autophagy Apoptosis

Urolithin a alleviates oxidative stress-induced senescence in nucleus pulposus-derived mesenchymal stem cells through SIRT1/PGC-1α pathway

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World Journal of Stem Cells 2021年第12期13卷 1928-1946页

作者： Peng-Zhi Shi Jun-Wu Wang Ping-Chuan Wang Bo Han Xu-Hua Lu Yong-xin Ren xin-min feng Xiao-Fei Cheng Liang Zhang Department of Orthopedic Dalian Medical UniversityDalian 116000Liaoning ProvinceChina Department of Orthopedics Clinical Medical College of Yangzhou UniversityYangzhou 225000Jiangsu ProvinceChina Department of Orthopedic Beijing Chaoyang HospitalCapital Medical UniversityBeijing 100020China Department of Orthopedics Changzheng Hospital of The Second Military Medical UniversityShanghai 200003China Department of Orthopedics First Affiliated Hospital of Nanjing Medical UniversityNanjing 210029Jiangsu ProvinceChina Department of Orthopedic Surgery Shanghai Key Laboratory of Orthopedics ImplantsShanghai Ninth People’s HospitalShanghai JiaoTong University School of MedicineShanghai 200011China

BACKGROUND In degenerative intervertebral disc(IVD),an unfavorable IVD environment leads to increased senescence of nucleus pulposus(NP)-derived mesenchymal stem cells(NPMSCs)and the inability to complete the differentiation from NPMSCs to NP cells,leading to further aggravation of IVD degeneration(IDD).Urolithin A(UA)has been proven to have obvious effects in delaying cell senescence and resisting oxidative *** To explore whether UA can alleviate NPMSCs senescence and to elucidate the underlying *** In vitro,we harvested NPMSCs from rat tails,and divided NPMSCs into four groups:the control group,H2O2 group,H2O2+UA group,and H2O2+UA+SR-18292 ***-associatedβ-Galactosidase(SA-β-Gal)activity,cell cycle,cell proliferation ability,and the expression of senescence-related and silent information regulator of transcription 1/PPAR gamma coactivator-1α(SIRT1/PGC-1α)pathway-related proteins and mRNA were used to evaluate the protective effects of *** vivo,an animal model of IDD was constructed,and Xrays,magnetic resonance imaging,and histological analysis were used to assess whether UA could alleviate IDD in *** We found that H2O2 can cause NPMSCs senescence changes,such as cell cycle arrest,reduced cell proliferation ability,increased SA-β-Gal activity,and increased expression of senescence-related proteins and *** UA pretreatment,the abovementioned senescence indicators were significantly *** further demonstrate the mechanism of UA,we evaluated the mitochondrial membrane potential and the SIRT1/PGC-1αpathway that regulates mitochondrial *** protected mitochondrial function and delayed NPMSCs senescence by activating the SIRT1/PGC-1α*** vivo,we found that UA treatment alleviated an animal model of IDD by assessing the disc height index,Pfirrmann grade and the histological *** In summary,UA could activate the SIRT1/PGC-1αsignaling pathway to protect mitochondrial function and alleviate ce

关键词： Urolithin A Mitochondrial function Oxidative stress Senescence Nucleus pulposus-derived Mesenchymal stem cells The silent information regulator of transcription 1/PPAR gamma coactivator-1αpathway

Quercetin ameliorates oxidative stress-induced senescence in rat nucleus pulposus-derived mesenchymal stem cells via the miR-34a-5p/SIRT1 axis

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World Journal of Stem Cells 2023年第8期15卷 842-865页

作者： Wen-Jie Zhao xin Liu Man Hu Yu Zhang Peng-Zhi Shi Jun-Wu Wang Xu-Hua Lu Xiao-Fei Cheng Yu-Ping Tao xin-min feng Yong-Xiang Wang Liang Zhang Graduate School Dalian Medical UniversityDalian 116044Liaoning ProvinceChina Department of Orthopedics Clinical Medical CollegeYangzhou UniversityYangzhou 225001Jiangsu ProvinceChina Department of Orthopedics Changzheng Hospital of The Second Military Medical UniversityShanghai 200003China Department of Orthopedic Surgery Shanghai Key Laboratory of Orthopedics ImplantsShanghai Ninth People’s HospitalShanghai Jiaotong University School of MedicineShanghai 200011China

BACKGROUND Intervertebral disc degeneration(IDD)is a main contributor to low back *** stress,which is highly associated with the progression of IDD,increases senescence of nucleus pulposus-derived mesenchymal stem cells(NPMSCs)and weakens the differentiation ability of NPMSCs in degenerated intervertebral discs(IVDs).Quercetin(Que)has been demonstrated to reduce oxidative stress in diverse degenerative *** To investigate the role of Que in oxidative stress-induced NPMSC damage and to elucidate the underlying *** In vitro,NPMSCs were isolated from rat ***-associatedβ-galactosidase(SA-β-Gal)staining,cell cycle,reactive oxygen species(ROS),realtime quantitative polymerase chain reaction(RT-qPCR),immunofluorescence,and western blot analyses were used to evaluated the protective effects of *** the relationship between miR-34a-5p and Sirtuins 1(SIRT1)was evaluated by dual-luciferase reporter *** explore whether Que modulates tert-butyl hydroperoxide(TBHP)-induced senescence of NPMSCs via the miR-34a-5p/SIRT1 pathway,we used adenovirus vectors to overexpress and downregulate the expression of miR-34a-5p and used SIRT1 siRNA to knockdown SIRT1 *** vivo,a puncture-induced rat IDD model was constructed,and X rays and histological analysis were used to assess whether Que could alleviate IDD in *** We found that TBHP can cause NPMSCs senescence changes,such as reduced cell proliferation ability,increased SA-β-Gal activity,cell cycle arrest,the accumulation of ROS,and increased expression of senescence-related *** abovementioned senescence indicators were significantly alleviated by Que *** decreased the expression levels of senescence-related proteins(p16,p21,and p53)and senescence-associated secreted phenotype(SASP),including IL-1β,IL-6,and MMP-13,and it increased the expression of *** addition,the protective effects of Que on cell senescence were partially reversed by mi

关键词： Quercetin Nucleus pulposus-derived mesenchymal stem cells Oxidative stress Senescence Intervertebral disc degeneration miR-34a-5p/SIRT1 pathway

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二自由度球面定点并联五杆机构的运动学分析

上海交通大学学报 2009年第5期43卷 830-833页

作者：方鑫许敏高峰上海交通大学振动冲击噪声国家重点实验室上海200240

以刚体空间运动学为基础,借助欧拉角法与球面解析几何方法建立由平行四杆驱动的二自由度球面定点五杆机构运动学模型,并对其运动学特性进行分析;运用三维CAD软件对运动过程进行仿真,将理论计算与仿真结果进行对比.结果表明,所建立的二... 详细信息

以刚体空间运动学为基础,借助欧拉角法与球面解析几何方法建立由平行四杆驱动的二自由度球面定点五杆机构运动学模型,并对其运动学特性进行分析;运用三维CAD软件对运动过程进行仿真,将理论计算与仿真结果进行对比.结果表明,所建立的二自由度球面定点五杆机构运动学模型准确有效.

关键词：球面定点并联机构运动学欧拉角法

非对称领结型纳米孔阵列的光透射特性（英文）

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光散射学报 2016年第3期28卷 285-292页

作者：孙鑫冯敏王斌曹学伟王玉芳南开大学物理科学学院天津300071

本文利用数值模拟的方法研究了两种不同的非对称领结型纳米孔结构的光学特性。对于偏置间隙的领结型纳米孔,其基模共振与孔的周长呈线性关系。并且,不同的间隙尺寸对间隙偏置的敏感度不同。对于间隙的一边的结构发生变化的领结型纳米孔... 详细信息

本文利用数值模拟的方法研究了两种不同的非对称领结型纳米孔结构的光学特性。对于偏置间隙的领结型纳米孔,其基模共振与孔的周长呈线性关系。并且,不同的间隙尺寸对间隙偏置的敏感度不同。对于间隙的一边的结构发生变化的领结型纳米孔,基模共振可以通过改变单个几何参量(剩余部分的高度h2)进行线性调制。另外,研究中还观察到了类Fabry-Perot共振的共振峰分裂。我们在这项工作中提出的这两种打破领结型纳米孔的对称性的方法可以灵活地对领结型纳米孔结构的共振进行操控。

关键词：领结型纳米孔异常光透射表面等离子体基元光学共振

Highly mass activity electrocatalysts with ultralow Pt loading on carbon black for hydrogen evolution reaction

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International Journal of minerals,Metallurgy and Materials 2025年第1期32卷 182-190页

作者： Shaorou Ke Yajing Zhao xin min Yanghong Li Ruiyu Mi Yangai Liu Xiaowen Wu minghao Fang Zhaohui Huang Engineering Research Center of ministry of Education for Geological Carbon Storage and Low Carbon Utilization of Resources Beijing Key Laboratory of Materials Utilization of Nonmetallic Minerals and Solid WastersNational Laboratory of Mineral MaterialsSchool of Materials Science and TechnologyChina University of Geosciences(Beijing)Beijing 100083China

Pt-based nanocatalysts offer excellent prospects for various ***,the low loading of Pt with excellent performance for efficient and stable nanocatalysts still presents a considerable *** this study,nanocatalysts with ultralow Pt content,excellent performance,and carbon black as support were prepared through in-situ ***~2-nm particles uniformly and stably dispersed on carbon black because of the strong s-p-d orbital hybridizations between carbon black and Pt,which suppressed the agglomeration of Pt *** unique structure is beneficial for the hydrogen evolution *** catalysts exhibited remarkable catalytic activity for hydrogen evolution reaction,exhibiting a potential of 100 mV at 100 mA·cm^(-2),which is comparable to those of commercial Pt/C *** activity(1.61 A/mg)was four times that of a commercial Pt/C catalyst(0.37 A/mg).The ultralow Pt loading(6.84wt%)paves the way for the development of next-generation electrocatalysts.

关键词： hydrogen evolution reaction ultralow platinum in-situ synthesis ultrasound

Enteromorpha prolifera polysaccharide-Fe(Ⅲ) complex promotes intestinal development as a new iron supplement

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Science China Life Sciences 2025年第1期 219-231页

作者： Yingying feng Yuying Wu Jialu Wang Zhenglin Dong Qian Yu Shuangshuang Xia Chunxue Liu Haihua Wang xin Wu Tianjin Institute of Industrial Biotechnology Chinese Academy of Sciences CAS Key Laboratory of Agro-ecological Processes in Subtropical Region Institute of Subtropical AgricultureChinese Academy of Sciences ANYOU Biotechnology Group Co.Ltd Qingdao Seawin Biotech Group Co. LTD

Iron is a crucial micronutrient, and its deficiency can have detrimental effects on the health of infants. Dietary polysaccharide-iron(Ⅲ) complexes(PICs) are promising for addressing iron deficiency due to their minimal adverse reactions and high iron absorption rate. This study aimed to investigate the effects of dietary Enteromorpha prolifera polysaccharide-Fe(Ⅲ) complex(EP-Fe) on newborns, using 3-day weaned piglets as the iron-deficiency model. Results showed that EP-Fe improved iron levels and promoted intestinal development in piglets. Transcriptome sequencing revealed that EP-Fe increased the survival of intestinal epithelial cells under hypoxia by upregulating the expression of genes that promote the development of the vascular system. Additionally, EP-Fe enhanced the mucosal barrier functions by inhibiting myosin light chain kinase(MLCK)/phosphorylated myosin light chain(p-MLC) signaling pathway to increase the expression of intestinal tight junction proteins. Furthermore, the 16S r RNA gene sequencing of gut microbiota showed that EP-Fe promoted the enrichment of Bacteroides＿fragilis and other gut microbes that can metabolize carbohydrates. In conclusion, EP-Fe is an effective iron supplement for newborns, and it can be developed as a comprehensive nutritional supplement.

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Metal-organic frameworks with dinuclear metal centers for synergistically boosting CO2 photoreduction

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Science China Chemistry 2025年第01期 201-208页

作者： Hui-feng Wang Wen-Jie Shi Yu-xin Yang Bao-Xia Dong Tong-Bu Lu Di-Chang Zhong School of Chemistry and Chemical Engineering Yangzhou University Institute for New Energy Materials and Low Carbon Technologies School of Materials Science and Engineering Tianjin University of Technology

Dinuclear metal synergistic catalysis （DMSC） has been proved powerful in boosting CO2reduction,while this has not been achieved in heterogeneous catalysts with long-range order *** this study,we,by introduction of metal catalytic centers into an indium-based metal-organic framework （MOF）,obtained a series of isostructural In-MOFs,including three MOFs with mononuclear metal catalytic center （M-In）,three MOFs with dinuclear homometallic catalytic centers （MM-In）,and three MOFs with dinuclear heterometallic catalytic centers (MM’-In,M/M’=Co（II）,Ni（II）,Zn（II）).It was found that all these MOFs can serve as catalysts for photochemical CO2reduction to *** with M-In,the catalytic activities of MM-In were obviously enhanced,and that of MM’-In was further significantly *** example,the CO yield of Co Zn-In reaches a record value of 12,700μmol g-1h-1,which is 4.0,8.1,9.4,and 18.6-fold higher than that of Co Co-In,Zn Zn-In,Co-In,and Zn-In,***,the catalytic activities of Co Ni-In and Ni Zn-In also show different fold increase than those of corresponding dinuclear homometallic and mononuclear metallic *** results of control experiments and theoretical calculation reveal that the higher catalytic activities of MM-In over M-In can be ascribed to the DMSC effect,and the higher catalytic activity of MM’-In over MM-In can be ascribed to the strengthened DMSC *** work reveals the relationship between DMSC effect and bimetal centers in CO2reduction,which provides new insights into the DMSC and paves a new way to design highly active catalysts for photochemical CO2reduction.

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氧化铁磁性纳米颗粒介导缺氧诱导因子1α shRNA质粒转染逆转A549/CDDP细胞对顺铂耐药的实验研究

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生物化学与生物物理进展 2010年第10期37卷 1090-1100页

作者：涂馨闵凌峰陈琼谢明萱何玲玲中南大学湘雅医院老年医学科呼吸内科长沙410008

评价了氧化铁磁性纳米颗粒作为缺氧诱导因子1α shRNA(HIF-1α shRNA)重组质粒载体进行体内体外转染的可行性及其逆转人肺腺癌耐药细胞株(A549/CDDP)顺铂耐性的效果,并初步探讨相关机制.在成功构建HIF-1α shRNA重组质粒后,分别利用氧... 详细信息

评价了氧化铁磁性纳米颗粒作为缺氧诱导因子1α shRNA(HIF-1α shRNA)重组质粒载体进行体内体外转染的可行性及其逆转人肺腺癌耐药细胞株(A549/CDDP)顺铂耐性的效果,并初步探讨相关机制.在成功构建HIF-1α shRNA重组质粒后,分别利用氧化铁磁性纳米颗粒及脂质体介导质粒转染A549/CDDP细胞及其移植瘤裸鼠动物模型,荧光显微镜检测转染效率,RT-PCR及细胞免疫化学检测转染后A549/CDDP细胞HIF-1α、多药耐药相关蛋白1(multidrug resistance1,MRP1)、肺耐药相关蛋白(lung resistance-related protein,LRP)mRNA及蛋白质表达,免疫组化检测转染后A549/CDDP移植瘤HIF-1α、MRP1及LRP蛋白表达,MTT法检测转染HIF-1α shRNA前后A549/CDDP细胞对顺铂的耐药性,并检测转染HIF-1α shRNA后A549/CDDP细胞移植瘤的肿瘤生长指数,HE染色检测纳米颗粒转染对裸鼠肝、肾及脑的组织学毒理作用.结果显示,氧化铁磁性纳米颗粒介导转染相对效率高于脂质体介导(P<0.01),HIF-1α shRNA转染A549/CDDP细胞后HIF-1α、MRP1、LRP mRNA及蛋白质表达下降,逆转A549/CDDP细胞对顺铂耐性82%,纳米颗粒介导HIF-1α shRNA转染A549/CDDP细胞移植瘤裸鼠动物模型后,移植瘤组织中HIF-1α、MRP1及LRP蛋白表达下降,转染HIF-1α shRNA后抑制移植瘤的生长,且与顺铂有协同效应,纳米颗粒转染后裸鼠肝、肾及脑组织无坏死.这些结果说明,纳米颗粒介导HIF-1α shRNA的体内体外转染效率高于脂质体,RNA干扰技术封闭HIF-1α基因可较大程度逆转耐顺铂人肺腺癌细胞的耐药性及其抑制瘤的生长,其作用可能与HIF-1α shRNA降低HIF-1α、MRP1及LRP表达有关,HIF-1α基因可作为逆转肺癌耐药治疗的有效靶点,磁性纳米颗粒介导HIF-1α shRNA质粒转染具有一定的生物安全性.

关键词：氧化铁磁性纳米颗粒肺肿瘤基因转染缺氧诱导因子1α(HIF-1d) RNA干扰