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Characterization of CD133^+ parenchymal cells in the liver:Histology and culture

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World Journal of Gastroenterology 2009年第39期15卷 4896-4906页

作者： Seiichi Yoshikawa Yoh zen Takahiko Fujii Yasunori Sato Tetsuo Ohta Yutaka Aoyagi Yasuni Nakanuma Department of Human Pathology Kanazawa University Graduate School of Medicine Division of Gastro-enterology and Hepatology Niigata University Graduate School of Medical and Dental Sciences Division of Pathology Kanazawa University Hospital Department of Gastrointestinal Surgery Kanazawa University Graduate School of Medicine

AIM： To reveal the characteristics of CD133^＋ cells in the liver, METHODS： This study examined the histological characteristics of CD133^＋ cells in non-neoplastic and neoplastic liver tissues by immunostaining, and also analyzed the biological characteristics of CD133^＋ cells derived from human hepatocellular carcinoma （HCC） or cholangiocarcinoma cell lines. RESULTS： Immunostaining reveated constant expression of CD133 in non-neoplastic and neoplastic biliary epithelium, and these cells had the immunophenotype CD133^＋/CK19^＋/HepPar-1. A small number of CD133^＋/CK19/HepPar-1^＋ cells were also identified in HCC and combined hepatocellular and cholangiocarcinoma. In addition, small ductal structures, resembling the canal of Hering, partly surrounded by hepatocytes were positive for CD133. CD133 expression was observed in three HCC （HUH7, PLC5 and HepG2） and two cholangiocarcinoma cell lines （HuCCT1 and CCKS1）. Fluorescence-activated cell sorting （FACS） revealed that CD133^＋ and CD133 cells derived from HuH7 and HuCCT1 cells similarly produced CD133^＋ and CD133 cells during subculture. To examine the relationship between CD133^＋ cells and the side population （SP） phenotype, FACS was performed using Hoechst 33342 and a monoclonal antibody against CD133. The ratios of CD133^＋/CD133 cells were almost identical in the SP and non-SP in HUH7. In addition, four different cellular populations （SP/CD133^＋, SP/CD133, nonP/CD133^＋, and non- SP/CD133） could similarly produce CD133^＋ and CD133- cells during subculture. CONCLUSION： This study revealed that CD133 could be a biliary and progenitor cell marker in vivo. However, CD133 alone is not sufficient to detect tumor-initiating cells in cell lines.

关键词： Cholangiocarcinoma Hepatocellular carcinoma Keratins Stem cells

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唐代世俗女性披帛探究

西部皮革 2024年第3期46卷 149-151页

作者：曹琪李欣盐城工业职业技术学院

魏晋时期，儒学统治地位动摇，为外来佛教的传播提供了空间，佛禅中理想化的审美追求促进了披帛的发展，开始广泛出现于壁画之中，直至唐代才在世俗女子的服饰中流行。披帛作为唐代女性服饰的重要组成部分，广泛流传于宫廷、贵族女性和... 详细信息

魏晋时期，儒学统治地位动摇，为外来佛教的传播提供了空间，佛禅中理想化的审美追求促进了披帛的发展，开始广泛出现于壁画之中，直至唐代才在世俗女子的服饰中流行。披帛作为唐代女性服饰的重要组成部分，广泛流传于宫廷、贵族女性和乐舞伎之中，承担着重要的装饰及审美功能，是女性身份的一种社会标志。唐王朝开放包容的社会环境、丰富多彩的艺术形式，极大地促进了服饰文化的进步，推动了披帛的流行与发展。

关键词：披帛服饰文化艺术形式

Risk factors and therapeutic response in Chinese patients with peptic ulcer disease

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World Journal of Gastroenterology 2010年第16期16卷 2017-2022页

作者： Shou-Wu Lee Teng-Yu Lee Hong-zen Yeh Chun-Fang Tung Yen-Chun Peng Division of Gastroenterology and Hepatology Department of Internal MedicineTaichung Veterans General Hospital

AIM:To assess the risk factors and the eff icacy of medications of patients with gastric and duodenal ulcers among Chinese patients in ***:Patients with peptic ulcers,diagnosed by upper endoscopy,were retrospectively collected between January 2008 and December *** differences were ***:Among all 448 cases,254(56.6%) and 194(43.4%) patients had gastric ulcers and duodenal ulcers *** with gastric ulcers were younger than those with duodenal *** more men existed,there was a female predominance in middle-aged *** with duodenal ulcers had a higher rate of Helicobacter pylori(***) infection(62.4% vs 43.3%,P = 0.001),and those with gastric ulcers owned a significantly higher amount of aspirin and nonsteroidal anti-inflammatory drug(NSAID) use(7.5% vs 1.5%,6.7% vs 2.1%,P = 0.001).Tobacco smoking and alcohol drinking had no different impact between these two ***-pump inhibitors and H2-receptor antagonists(H2RA) were effective,but significantly less so in cases with duodenal ulcers receiving H2RAs,or in those with *** infection and a history of NSAID ***:Patients with gastric ulcers had lower *** infection but more aspirin or NSAID *** therapy was ineffective in gastric ulcers underwent H2RA treatment,and cases combined *** infection and NSAID use.

关键词： Epidemiology Peptic ulcer disease Therapy

Effects of neutral salt and alkali on ion distributions in the roots,shoots,and leaves of two alfalfa cultivars with differing degrees of salt tolerance

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Journal of Integrative Agriculture 2017年第8期16卷 1800-1807页

作者： WANG Xiao-shan REN Hai-long WEI zen-wu WANG Yun-wen REN Wei-bo Department of Grassland Science College of Animal Science and TechnologyYangzhou UniversityYangzhou 225009P.R.China Institute of Grassland Research Chinese Academy of Agricultural SciencesHohhot 010000P.R.China Department of Grassland Science College of Animal Science and TechnologyChina Agricultural UniversityBeijing 100193P.R.China

The effects of neutral salt and alkali on the ion distribution were investigated in two alfalfa （Medicago sativa L.） cultivars, including Zhongmu 1, a high salt-tolerant cultivar, and Algonquin, a low salt-tolerant cultivar. The alkali stress expressed more serious growth inhibition than the neutral salt stress at the same Na＋ concentration. Compared with Algonquin, Zhongmu 1 did not exhibit a higher alkali tolerance under the Na2CO3-NaHCO3 treatment with the low Na＋ concentration （50 mmol L-l）. The alkali increased the accumulation of Na＋, Ca2＋, and Mg2＋ in the root and changed the Ca2＋ and Mg2＋ balance in the entire alfalfa plant. The salt and alkali stresses decreased the K＋ and Fe3＋ contents of the roots and leaves, the root Mn2＋ content, and the shoot Zn2＋ content, but they increased the Fe3＋ accumulation of the shoots, the shoot and leaf Cu2＋ contents, and the leaf Zn2＋ content in both alfalfa cultivars. Based on the results obtained under the conditions of this experiment, we found that the salt and alkali stresses reduced the plant growth in both alfalfa cultivars, while the alkali caused a stronger stress than the neutral salt in alfalfa. Thus, we conclude that under hydroponic conditions, the deleterious effects of the alkali on plants are due to the distribution change of some trophic ion balance in the roots, shoots, and leaves of the plants by causing of Na＋, CO3^2-, and/or HCO3- stresses.

关键词： alfalfa ion distribution neutral salts alkali stress

Enhancing Li-ion conduction and mechanical properties via addition of fluorine-containing metal–organic frameworks in allsolid-state cross-linked hyperbranched polymer electrolytes

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Nano Research 2022年第10期15卷 8946-8954页

作者： Wen Wen Qinghui zeng Pingping Chen xin Wen Zhenfeng Li Yu Liu Jiazhu Guan Anqi Chen Wei Liu Liaoyun Zhang School of chemical sciences University of Chinese Academy of SciencesBeijing 100049China Research Institute of Petroleum Exploration&Development(RIPED) PetroChinaBeijing 100083China

Poly(ethylene oxide)(PEO)-based solid polymer electrolytes(SPEs)are commonly used in lithium metal batteries(LMBs)for their good Li-salt solvating ability and easy ***,the relatively low Li-ion conduction ability hinders their further *** this work,a novel hyperbranched-polyether-type composite solid polymer electrolyte(CSPE)is prepared via a quick cross-linking reaction between aldehyde-terminated polyethylene glycol(PEG)and hyperbranched poly(ethylene imine)(HPEI)in the presence of lithium salt and fluorine-containing Zr-based metal–organic framework(MOF)UiO-66-(F)_(4).The hydrogen bonds between the fluorine atoms and amino groups in the electrolyte help to the better dispersion of UiO-66-(F)_(4) in the polymer matrix,which is beneficial to solving the problem of aggregation of ***,the CSPEs with the functional MOF fillers show improvements in both electrochemical and mechanical ***,the Li-ion transference number(t)is considerably enhanced from 0.23 to ***-solid-state LMBs based on the CSPE also present good cycling performances.A high specific discharge capacity of 141.4 mAh·g^(−1) is remained after 200 cycles at 0.2 *** study not only provides an effective synthesis method of the cross-linked hyperbranched polymer electrolyte,but also puts forward a new strategy for uniform dispersion of inorganic fillers in CSPEs.

关键词： hyperbranched cross-linked polymer electrolyte all-solid-state metal-organic frameworks composite lithium metal batteries

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地震引起的边坡灾害风险评估(英文)

岩石力学与工程学报 2008年第12期27卷 2488-2493页

作者：陈光齐 zen Kouki KASUYA Yuki 九州大学土木与结构工程系

2005年日本福冈县7级地震引起大量边坡破坏。震中附近的志贺岛的环岛公路由于其上方边坡崩塌导致交通中断达1.5 a之久。其原因是崩塌边坡上方出现了较大的沉降裂缝,有可能受将来的暴雨或地震的触发再次导致边坡崩塌。根据钻孔资料及实... 详细信息

2005年日本福冈县7级地震引起大量边坡破坏。震中附近的志贺岛的环岛公路由于其上方边坡崩塌导致交通中断达1.5 a之久。其原因是崩塌边坡上方出现了较大的沉降裂缝,有可能受将来的暴雨或地震的触发再次导致边坡崩塌。根据钻孔资料及实验室实验结果,对该边坡未来可能发生的崩塌规模产生3种意见:小规模表层崩塌(A)、中规模中层崩塌(B)及大规模深层崩塌(C)。而边坡修复费用则根据所想定的可能崩塌规模从几千万日元到几十亿日元不等。为了合理决定对策规模,对3种可能的崩塌规模分别进行风险评估。结果表明,大规模深层崩塌(C)由于发生概率较小使得其风险远远小于小规模表层崩塌(A)和中规模中层崩塌(B),因而在修复中可以不予考虑。另外,虽然小规模表层崩塌(A)和中规模中层崩塌(B)的风险相当,但考虑到对策效果(加固后的风险减少与投入的加固资金费用之比),认为以小规模表层崩塌为对象制定修复计划较为合理,但在部分区域可考虑有关针对中规模中层崩塌的对策。本提案被相关部门所采用。

关键词：边坡工程风险评估地震灾害非连续变形分析

The protective role of myeloid-derived suppressor cells in concanavalin A-induced hepatic injury

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Protein & Cell 2014年第9期5卷 714-724页

作者： Wenli Diao Fangfang Jin Bing Wang Chen-Yu Zhang Jiangning Chen Ke zen Limin Li Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology State Key Laboratory of Pharmaceutical Biotechnology School of Life Sciences Nanjing University Nanjing 210093 China People's Liberation Army 404 Hospital Weihai 264200 China

The mechanism underlying T cell-mediated fulminant hepatitis is not fully understood. In this study, we investigated whether myeloid derived suppressor cells （MDSCs） could prevent the concanavalin A （ConA）- induced hepatitis through suppressing T cell proliferation. We observed an increase in the frequencies of MDSCs in mouse spleen and liver at early stage of ConA treatment, implicating that the MDSCs might be involved in the initial resistance of mice against ConA- mediated inflammation. Subpopulation analysis showed that the MDSCs in liver of ConA-induced mice were mainly granulocytic MDSCs. Adoptive transfer of the bone marrow-derived MDSCs into ConA-treated mice showed that the MDSCs migrated into the liver and spleen where they suppressed T cell proliferation through ROS pathway. In addition, the frequencies of MDSCs in mice were also significantly increased by the treatment with immune suppressor glucocorticoids. Transfer of MDSCs into the regulatory T cell （Treg）- depleted mice showed that the protective effect of MDSCs on ConA-induced hepatitis is Treg-independent. In conclusion, our results demonstrate that MDSCs possess a direct protective role in T cell-mediated hepatitis, and increasing the frequency of MDSCs by either adoptive transfer or glucocorticoid treatment represents a potential cell-based therapeutic strategy for the acute inflammatory disease.

关键词： myeloid derived suppressor cells T cell-mediated hepatitis ROS glucocorticoids concanavalin A(ConA) adoptive transfer glucocorticoid treatment

Studies on the structure of xindongnins A and B

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Acta Chimica Sinica(English Edition) 1985年第2期3卷 127-134页

作者： SUN Han-Dong LIN Zhong-Wen FU Jian Kunming Institute of Botany,Academia Sinica,Kunming,ChinaZHENG xin-Rong GAO zen-Yi Henan Institute of Biology,Academy of Henan Province Kunming Institute of Botany Academia Sinica Kunming China Henan Institute of Biology Academy of Henan Province

Two new diterpenoids,xindongnins A (1) and B (2) with an ent-kaurene skeleton wereisolated from the leaves of Rabdosia rubescens (Hemsl.) Hara collected in xinyang of Henan,andtheir structures were deduced from spectr... 详细信息

关键词： Studies on the structure of xindongnins A and B

Productivity and the Survival Rate after Winter Management of Seven Tropical Forage Accessions in Subtropical Region of China

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Agricultural Sciences 2017年第5期8卷 451-464页

作者： Chengxin He ling Mo Yuqing Huang Zhongfeng Zhang Michael Hare Danjuan zen Gaozhong Pu Guangxi Key Laboratory of Plant Conservation and Restoration Ecology in Karst Terrain Guangxi Institute of Botany CAS Guilin China Faculty of Agriculture Ubon Ratchathani University Ubon Ratchathani Thailand

Forage production and quality of five tropical grass accessions (Guimu-1 hybrid elephant grass (PG1), Mulato II (M II), Ubon paspalum (PU), hybrid elephant grass (PH) and Reyan 11 paspalum (PR11)) and two tropical legume accessions (Reyan 5 stylo (SR5) and Ubon stylo (SU)) were evaluated in a field experiment in a subtropical area with hot summers and cold winters in Guangxi, China. Three forage stubble cover managements: no cover (CK), dry mass cover (MC) and plastic cover (PC) were applied at the end of the summer period to evaluate cold tolerance and accession survival over the winter. Photosynthesis measurements were taken from the forages in mid-summer. The results showed that PG1 accession produced significantly higher dry matter yields (67.0 t·ha-1) than the other grass and legume accessions. Legume accessions SU and SR5 produced much less dry matter (6.4 - 7.2 t·ha-1) compared to the grasses. M II, PU and PR11 contained the largest proportion of leaf. PG1 and PH showed good cold tolerance (survival rate >85%) under -1°C conditions without any cover management. PR11 had better cold tolerance than PU. M II exhibited very poor winter survival under no cover and with massive cover, and only survived well under plastic cover. The two stylo accessions died completely under all winter cover management treatments. In the growing season, under a given photosynthesis photon flux density (PPFD) = 1500 μmol m-2 s-1, the net photosynthesis rate Pn of all the seven accessions was above 28 μmol m-2 s-1, of which hybrid elephant grass and M II were above 42 μmol m-2 s-1. Though these 7 tropical accessions yielded high production in the grow season, stylo and M II are sensitive to cold in subtropical region of Guangxi. The result suggests that PG1 and PU are good accessions and can be used as productive perennial pasture, while stylo and M II are simply considered as annual one. Additionally, if paspalum was trained in cool region before being introduced to subtropical regio

关键词： Cold Tolerance Production Photosynthesis Tropical Grasses Winter