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RESEARCH ON A NEW FORGING TECHNIQUE—SHORT-HOLLOW-RAPID DIE FORGING

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Journal of Central South University 1997年第1期9卷 46-48页

作者： zen Sumin(Southwest Aluminum Fabrication Plant, Chongqing 631326, China) Southwest Aluminum Fabrication Plant Chongqing China

A new forging technique short-hollow--rapid die forging, used in Ti-Al alloys was *** with conventional method, the new method can improve the crystalline structure properties of theforged piece, reduce the average forging pressure by 20% to 30%, lessen the thickness deviation by 5 mm,and prolong the service life of the dies by 2 to 4 times.

关键词： rapid die forging Ti-Al alloy hollow

MicroRNA-29a modulates axon branching by targeting doublecortin in primary neurons

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Protein & Cell 2014年第2期5卷 160-169页

作者： Hanqin Li Susu Mao Haitao Wang Ke zen Chenyu Zhang Liang Li Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology State Key Laboratory of Pharmaceutical Biotechnology Nanjing University School of Life Sciences Nanjing 210093 China Department of Pharmacy 306th Hospital of PLA Beijing 100101 China

MicroRNAs （miRNAs） are endogenously expressed small, non-coding transcripts that regulate protein expression. Substantial evidences suggest that miRNAs are enriched in central nervous system, where they are hypothesized to play pivotal roles during neural devel- opment. In the present study, we analyzed miRNAs expression in mice cerebral cortex and hippocampus at different developmental stages and found miR-29a increased dramatically at postnatal stages. In addition, we provided strong evidences that miR-29a is enriched in mature neurons both in vitro and in v/vo. Further investigation demonstrated that the activation of gluta- mate receptors induced endogenous miR-29a level in primary neurons. Moreover, we showed that miR-29a directly regulated its target protein Doublecortin （DCX） expression, which further modulated axon branching in primary culture. Together, our results suggested that miR-29a play an important role in neuronal development of mice cerebrum.

关键词： MiRNAs are approximately 21 nucleotide non-codingtranscripts that are derived from hairpin precursors which

Immune modulatory function of abundant immune-related microRNAs in microvesicles from bovine colostrum

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Protein & Cell 2013年第3期4卷 197-210页

作者： Qi Sun Xi Chen Jianxiong Yu Ke zen Chen-Yu Zhang Liang Li Jiangsu Engineering Research Center for microRNA Biology and Biotechnology State Key Laboratory of Pharmaceutical BiotechnologyNanjing University School of Life SciencesNanjing 210093China Department of Virology University of California School of Public HealthBerkeleyCA 94720USA

Colostrum provides essential nutrients and immunologically active factors that are beneficial to *** previous work demonstrated that milk contains large amounts of miRNA that is largely stored in milk-derived microvesicles(MVs).In the present study,we found that the MVs from colostrum contain signifi cantly higher levels of several immune-related *** hypothesized that the colostrum MVs may transfer the immune-related miR-NAs into cells,which contribute to its immune modulatory *** isolated colostrum MVs by ultracentrifugation and demonstrated several immune modulation features associated with *** also provide evidence that the physical structure of milk-derived MVs is essential for transfer miRNAs and following immune modulation ***,we found that colostrum powder-derived MVs also contains higher levels of immune-related miRNAs that display similar immune modulation *** together,these results show that MV-containing immunerelated miRNAs may be a novel mechanism by which co-lostrum modulates body immune response.

关键词： colostrum miRNAs microvesicles immune modulation

Synthesis Characterization Non-isothermal Kinetics of the Thermal Decomposition and Redox Properties Derived from Copper(Ⅱ) Binuclear Coordination Compound of 1,4-Bis-(1'-Phenyl-3'-Methyl-5'-Pyrazolone-4')-1,4-Butanedione

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Chinese Chemical Letters 1997年第5期8卷 455-458页

作者： Cun SHAN Dian zen JIA Xi XIA(Department of Chemistry,, xinjiang University, Urumqi,830046). Department of Chemistry Xinjiang University Urumqi 830046 China

The paper reports the synthetic procedure and character of Copper(II) binuclearcoordination compound of 1,4-bis-(1'-phenyl-3'-methyl-5'-pyrazolone Thenon-isothermal kinetics of thermal decomposition of the complex has been stUdied from the TG-DTGcurves by means of the Achar et al. and Coats-Redfern methods,the most probab1e kinetic equation canbe expressed as dofdtrAe -E / RT * l /(2Q).The corresponding kinetic compensation effect expressions arefound to be lnuA=0. 1794E+0. *** non-isothermal thermal decomposition process of the complex isone-dimensional *** electrochemical studies of the complex(Cu2L'2)from cyclic voltamrnetriccurves by means of powder microelectrodes technique'',shows one two-electron irreversible process.

关键词： Methyl-5 Phenyl-3 Pyrazolone-4 Synthesis Characterization Non-isothermal Kinetics of the Thermal Decomposition and Redox Properties Derived from Copper Binuclear Coordination Compound of 1,4-Bis

Biopolymer-assisted synthesis of yttrium oxide nanoparticles

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Particuology 2014年第3期12卷 19-23页

作者： Hakan Kaygusuz Gkhan Bilir Filiz Tezcan F.Bedia Erim Gnl zen Department of Chemistry Istanbul Technical UniversityMaslak 34469IstanbulTurkey Department of Physics Istanbul Technical UniversityMaslak 34469IstanbulTurkey

Yttrium oxide nanopowder was prepared by a novel technique using an alginate biopolymer as a precursor. The technique is based on thermal decomposition of an yttrium alginate gel, which is produced in the form of beads by ionic gelation between the yttrium solution and sodium alginate. The effect of post-annealing temperature on the particle size of the nanocrystals was investigated at various tempera- tures. The products were characterized using X-ray diffraction, scanning electron microscopy, and atomic force microscopy. The size of the nanocrystalline Y2O3 particles varied from 22.7 to 38.7 nm, depending on the annealing temperature and time. The grain size distribution （GSD） was also determined. The GSD became more non-symmetrical as the annealing temperature increased, and the width of the distributions for the powders produced using the alginate method was less affected by heat treatment. This alginate method was compared with the conventional glycine combustion method, on the basis of particle size. The particles obtained using the proposed technique were smaller than those obtained using the combustion method. Alginate-assisted thermal decomposition is therefore an easy and cost-effective method for preparing nanosized Y2O3 crystals.

关键词： Y2O3 Nanoparticle Thermal decomposition Alginate Grain size distribution

Selective secretion of microRNA in CNS system

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Protein & Cell 2013年第4期4卷 243-247页

作者： Qipeng Zhang Jie Xu Qun Chen Xi Chen Ke zen Chen-Yu Zhang Jiangsu Engineering Research Center for microRNA Biology and Biotechnology State Key Laboratory of Pharmaceutical BiotechnologySchool of Life SciencesNanjing UniversityNanjing 210093China

EXTRACELLULAR miRNAs ARE PRESENT IN VARIOUS BODY FLUIDS MicroRNAs(miRNAs)are a diverse class of endogenous small non-coding RNAs,which posttranscriptionally regulate gene expression by interacting with their binding sites in target ***,several studies have demonstrated that miRNAs are also detectable outside cells,and these miRNAs may be called extracellular *** miRNAs are found in various body fluids,including plasma and serum(Chen et al.,2008),saliva(Park et al.,2009),amniotic fl uid(Weber et al.,2010),Bronchial lavage(Molina-Pinelo et al.,2012;Weber et al.,2010),sputum(Xie et al.,2010),tears and urine(Weber et al.,2010),bile(Shigehara et al.,2011),seminal plasma(Wang et al.,2011),breast milk(Zhou et al.,2012),peritoneal fluid(Chen et al.,2012a).Finding the extracellular miRNAs extended the research field of miRNAs,and got a lot of fruitful research fi ndings.

关键词： al. Weber endogenous

Identification of Ebola virus microRNAs and their putative pathological function

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Science China(Life Sciences) 2014年第10期57卷 973-981页

作者： LIANG HongWei ZHOU Zhen ZHANG SuYang zen Ke CHEN Xi ZHANG ChenYu Jiangsu Engineering Research Center for microRNA Biology and Biotechnology State Key Laboratory of Pharmaceutical BiotechnologySchool of Life Sciences Nanjing University

Ebola virus(EBOV),a member of the filovirus family,is an enveloped negative-sense RNA virus that causes lethal infections in humans and ***,more than 1000 people have been killed by the Ebola virus disease in Africa,yet no specific treatment or diagnostic tests for EBOV are *** this study,we identified two putative viral microRNA precursors(pre-miRNAs)and three putative mature microRNAs(miRNAs)derived from the EBOV *** production of the EBOV miRNAs was further validated in HEK293T cells transfected with a pcDNA6.2-GW/EmGFP-EBOV-pre-miRNA plasmid,indicating that EBOV miRNAs can be produced through the cellular miRNA processing *** also predicted the potential target genes of these EBOV miRNAs and their possible biological ***,this study reports for the first time that EBOV may produce miRNAs,which could serve as non-invasive biomarkers for the diagnosis and prognosis of EBOV infection and as therapeutic targets for Ebola viral infection treatment.

关键词： microRNA Ebola virus function

Emulsion‑based evolution of Escherichia coli for higher growth yield on D‑xylose identifies central role of cyclic AMP

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Systems Microbiology and Biomanufacturing 2023年第4期3卷 730-738页

作者： James S.Orr Edwin zen Xiaoyi Wang Christopher V.Rao DOE Center for Advanced Bioenergy and Bioproducts Innovation UrbanaILUSA Department of Chemical and Biomolecular Engineering University of Illinois at Urbana-Champaign600 S.Mathews Ave.UrbanaIL 61801USA Core Facility Center The First Affiliated Hospital of Nanjing Medical UniversityNanjingChina

D-xylose is an abundant sugar found in plant biomass and can be used as a renewable feedstock for the microbial production of diverse biofuels and ***,D-xylose metabolism is slow in many industrial microorganisms,at least as compared to glucose *** surprisingly,a number of approaches have been developed for improving D-xylose metabolism in diverse *** this work,we applied a previously developed evolution strategy based on media-in-oil emulsions for improving the growth yield of Escherichia coli NCM3722 on *** 30 rounds of evolutions,we isolated multiple mutants with increased growth yield on *** addition,we also observed similar increases in the growth *** mutants were selected for whole-genome *** mutants had an amber stop mutation in adenylate cyclase,which truncates nearly 60%of the ***,the ability of this mutant to grow on xylose indicated that truncated enzyme,lacking the C-terminal regulatory domain,is still *** other mutant had a point mutation in the cyclic AMP receptor protein(CRP),near the high affinity binding site for cyclic *** mutations,when intro-duced into wild type ***,were able to increase the growth yields at levels similar to the isolated *** addition to D-xylose,these mutant strains and their genetic mimics also exhibited higher growth rates and yields on glucose,lactose,and *** results suggest that the improved growth rates and yields are due to changes in the production and sensing of intracellular cyclic AMP concentrations and also suggest native concentrations are suboptimal with respect to the growth rate and yield under the growth conditions ***,these results may prove useful for engineering strains of *** for high-density fermentations or protein production.

关键词： Escherichia coli Xylose cAMP Adaptive laboratory evolution

Varying coefficient analysis for indeterminate species interactions with non-parametric estimation, exemplifying with a fig-fig wasp system

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Chinese Science Bulletin 2011年第24期56卷 2545-2552页

作者： SHI Lei WANG RuiWu ZHU Lixing zen WeiMing XU WangLi ZHENG Qi Statistics and Mathematics School Yunnan University of Finance and Economics Kunming 650221 China Ecology Conservation and Environment Center (ECEC) State Key Laboratory of Genetic Resources and Evolution Kunming Institute of Zoo-logy Chinese Academy of Sciences Kunming 650223 China Department of Mathematics Hong Kong Baptist University Hong Kong China Scientific and Technical Department Yunnan University of Nationalities Kunming 650031 China Statistics College RenMing University of China Beijing 100080 China

Research on species interactions has generally assumed that species have a fixed interaction and therefore linear or non-linear parametric regression models (e.g. exponential, logistic) have been widely used to describe the species interaction. However, these models that describe the relationship between interacting species as a specific functional response might not be appropriate for real biological communities, for instance, in a chaotic system, when the species relationship varies among different situations. To allow a more accurate description of the relationship, we developed a species correlation model with varying coefficient analysis, in which a non-parametric estimation is applied to identify, as a function of related factors, variation in the correlation coefficient. This was applied to a fig-fig wasp model system. When the effect of the factors on the relationship can be described with parameters, the new method reduces to traditional parametric correlation analysis. In this way, the new method is more general and flexible for empirical data analyses, but different by allowing investigation of whether a species interaction varies with respect to factors, and of the factors that maintain or change the species interaction. This method will have important applications in both theoretical and applied research (e.g. epidemiology, community management).

关键词：相互作用系数非参数估计混沌系统无花果物种非线性参数例证小蜂