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Localization and secretory pathways of a 58K-like protein in multi-vesicular bodies in callus of Arabidopsis thaliana

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Science China(Life Sciences) 2008年第9期51卷 827-832页

作者： xu Shan QIAN Jie SONG xin ZHU jian School of Life Science and Technology Tongji UniversityShanghai 200092China Laboratory of Electron Microscopy Faculty of Basic Medicine ScienceSecond Military Medical UniversityShanghai 200433China

Multi-vesicular bodies in endocytosis and protoplasts are special cellular structures that are consid-ered to be originated from invagination of plasma membranes. However, the genesis and function of multi-vesicular bodies, the relationship with Golgi bodies and cell walls, and their secretory pathways remain controversial and ambiguous. Using a monoclonal antibody against an animal 58K protein, we have detected, by Western blotting and confocal microscopy, that a 58K-like protein is present in the calli of Arabidopsis thaliana and Hypericum perforatum. The results of immuno-electron microscopy showed that the 58K-like protein was located in the cisternae of Golgi bodies, secretory vesicles, multi-vesicular bodies, cell walls and vacuoles in callus of Arabidopsis thaliana, suggesting that the multi-vesicular bodies may be originated from Golgi bodies and function as a transporter carrying substances synthesized in Golgi bodies to cell walls and vacuoles. It seems that multi-vesicular bodies have a close relationship with the development of the cell wall and vacuole. The possible secretory pathways of multi-vesicular bodies might be in exocytosis, in which multi-vesicular bodies carry sub-stances to the cell wall for its construction, and in endocytosis, in which multi-vesicular bodies carry substances to the vacuole for its development, depending on what they carry and where the materials are transported. We hence propose that there is more than one pathway for the secretion of multi-vesicular bodies. In addition, our results provided a paradigm that a plant molecule, such as the 58k-like protein in callus of Arabidopsis thaliana, can be detected using a cross-reactive monoclonal antibody induced by an animal protein, and illustrate the existence of analog molecules in both animal and plant kingdoms.

关键词： 58K-like protein Arabidopsis thaliana localization multi-vesicular bodies secretory pathway

Effect of emodin and sandostatin on metabolism of eicosanoids in acute necrotizing pancreatitis

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World Journal of Gastroenterology 2000年第2期6卷 293-294页

作者： jian xin Wu Jia Yu xu Yao Zong Yuan Department of Gastroenterology Shanghai First People’s HospitalShanghai 200080China Ruijin Hospital Shanghai Second Medical UniversityShanghai 200025China

INTRODUCTIONIn order to study the therapeutic mechanisms ofemodin,an extract of Rhubarb （Rhizoma et RadixRhei,a traditional Chinese herbal medicine）,andsandostatin in the treatment of acute necrotizingpancreatitis （ANP）,we used the two drugs in ratmodels of the disease and observed the changes ofplasma thromboxane-2（TXB2）,6-keto-prostaglandin F1α(6-keto-PGF1α)and prostaglandinE2（PEG2）.

关键词： pancreatitis eicosanoids metabolism emodin sandostatin

Location of Caspase 3-like Protease in the Development of Sieve Element and Tracheary Element of Stem in Cucurbita moschata

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Journal of Integrative Plant Biology 2008年第12期50卷 1499-1507页

作者： Xia Hao Jie Qian Shan xu xin Song jian Zhu School of Life Science and Technology Tongji University Shanghai 200092 China

The casepase is considered to regulate the process of programmed cell death in the development of organisms. In this study, caspase 3-like protease was detected by immunohistochemistry and immunoelectron microscopy during the development of sieve element and tracheary element of stem in Cucurbita moschata Duch. Antibody with brown color （under light microscopy） and gold particles （under transmission electron microscopy） for detecting caspase 3-like protease was mainly displayed in inner phloem, external phloem and xylem in the region close to procambium. From the results it was considered that caspase 3-like protease did exist in vascular elements and played different roles during the development of sieve and tracheary elements, and different types of programmed cell death might be carried out. The caspase 3-like protease mainly participated in making cytoplasmic streaming cease and in degrading P-protein bodies; however, it rarely participated in the function for signal transferring in the developmental sieve element. However, it might induce calcium accumulation for rupturing the tonoplast in the signal of PCD in the developmental tracheary element.

关键词： caspase 3-like protease Cucurbita moschata immunoelectron microscopy P-protein bodies programmed cell death.

A demand side controller of electrolytic aluminum industrial microgrids considering wind power fluctuations

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Protection and Control of Modern Power Systems 2022年第1期7卷 738-750页

作者： xin Ding jian xu Yuanzhang Sun Siyang Liao Jingwen Zheng School of Electrical Engineering and Automation Wuhan UniversityWuhan 430072China School of Mechanical Engineering University of Shanghai for Science and TechnologyShanghaiChina State Grid Hubei Electric Power Research Institute State Grid Hubei Electric Power Co.Ltd.WuhanChina

Direct wind power purchase for large industrial users is a meaningful way to improve wind power consumption and decrease industrial production ***-term wind power fluctuations may lead to large-scale wind power curtailment *** promote use of wind energy,a demand side control method is proposed based on output regulator theory for a grid-connected industrial microgrid with electrolytic aluminum loads to continuously track and respond to wind power *** control model of the EALs and the dominant frequencies of the wind power fluctuation signals are analyzed and incorporated into the demand side control *** feedback control signals with active power deviations on the tie-line are used to design the demand side *** are conducted for an actual industrial microgrid to validate the feasibility and effectiveness of the proposed *** results demonstrate that the proposed controller based on output regulator theory is able to effectively track wind power fluctuations.

关键词： Wind power fluctuations Electrolytic aluminum load Demand side control Output regulator theory Industrial microgrid

Umbilical cord blood mercury levels in China

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Journal of Environmental Sciences 2013年第2期25卷 386-392页

作者： Meiqin Wu Chonghuai Yan jian xu Wei Wu Hui Li xin Zhou MOE-Shanghai Key Laboratory of Children's Environmental Health Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine

Mercury （Hg） is a well-known neurotoxicant. Hg exposure at high levels can harm individuals of all ages. Even low level exposure to Hg can damage the brain of fetuses and young children, and affect their central nervous system and cognitive development. The aims of our study were to measure total Hg levels in infant umbilical cord blood and to investigate the risk factors associated with total Hg cord blood levels in various cities in China. Our goal was to provide clues for the prevention of Hg exposure in utero. The results indicated that the average cord blood mercury levels （CBMLs） were （1.81 ± 1.93） μg/L, which were lower than those found in most previous studies. The concentrations also differed according to geographic region. The CBMLs were not only associated with family economic and living conditions, but also with diet in pregnant women, especially the intake of marine fish, shellfish, poultry, formula milk and fruits.

关键词： infant mercury risk factor umbilical cord blood

Coordinated Frequency Control for Isolated Power Systems with High Penetration of DFIG-Based Wind Power

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CSEE Journal of Power and Energy Systems 2024年第4期10卷 1399-1414页

作者： xin Ding Wei Lin jian xu Yuanzhang Sun Liangzhong Yao Beilin Mao School of Electrical Engineering and Automation Wuhan UniversityWuhan 430072China Department of Electrical Engineering and Computer Science Case Western Reserve UniversityClevelandOH 44106 USA

This paper proposes a coordinated frequency control scheme for emergency frequency regulation of isolated power systems with a high penetration of wind *** proposed frequency control strategy is based on the novel nonlinear regulator theory,which takes advantage of nonlinearity of doubly fed induction generators(DFIGs)and generators to regulate the frequency of the power *** deviations and power imbalances are used to design nonlinear feedback controllers that achieve the reserve power distribution between generators and DFIGs,in various wind speed *** effectiveness and dynamic performance of the proposed nonlinear coordinated frequency control method are validated through simulations in an actual isolated power grid.

关键词： Frequency control isolated power systems nonlinear feedback nonlinear regulator theory wind power

High activity in catalytic cracking of large molecules over micro-mesoporous silicoaluminophosphate with controlled morphology

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Science China Chemistry 2010年第11期53卷 2279-2284页

作者： QI jian ZHAO TianBo xu xin LI FengYan SUN GuiDa Institute for Chemical Physics Beijing Institute of TechnologyBeijing 100081China Department of Applied Chemistry Beijing Institute of Petrochemical TechnologyBeijing 102617China

A novel micro-mesoporous silicoaluminophosphate(MUS-5) with controlled morphology has been first synthesized in a two-step *** physical properties of the silicoaluminophosphate were characterized using XRD,SEM,TEM,nitrogen adsorption-desorption and NH3-TPD *** the pH value of the solution system was varied in the range from 2.0 to 5.0,three different morphologies of silicoaluminophosphate including chain-like,flower-like and barrel-like morphology were *** tests showed that the silicoaluminophosphate exhibited higher catalytic activity compared with the conventional microporous SAPO-5 under the same conditions for catalytic cracking of 1,3,5-triisopropylbenzene heavy *** remarkable catalytic reactivity was mainly attributed to the presence of the hierarchical porosity in the silicoaluminophosphate catalyst.

关键词： micro-mesoporous silicoaluminophosphate controlled morphology catalytic cracking large molecule

直接Z-型BAs/Ga_(2)SO范德华异质结光催化分解水研究

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原子与分子物理学报 2025年第6期42卷 10-16页

作者：许康黄欣杨志红南京邮电大学电子与光学工程学院、柔性电子(未来技术)学院南京210023 南京邮电大学理学院南京210023

基于第一性原理计算,系统研究了BAs/Ga_(2)SO异质结的光催化分解水性能.结果表明:BAs/Ga_(2)SO异质结具有良好的热力学稳定性并表现出交错的能带排列方式,带隙值为0.73 eV,界面处存在从BAs指向Ga_(2)SO的内建电场使其形成了直接Z-型异质... 详细信息

基于第一性原理计算,系统研究了BAs/Ga_(2)SO异质结的光催化分解水性能.结果表明:BAs/Ga_(2)SO异质结具有良好的热力学稳定性并表现出交错的能带排列方式,带隙值为0.73 eV,界面处存在从BAs指向Ga_(2)SO的内建电场使其形成了直接Z-型异质结,能够有效分离光生电子-空穴对.另外,异质结的带边位置可以跨越水的氧化还原电位,析氢反应发生在BAs侧而析氧反应发生在Ga_(2)SO侧.最后,基于吉布斯自由能变化计算,发现在有光照时,析氢和析氧反应在BAs/Ga_(2)SO异质结表面可自发进行.以上结果表明BAs/Ga_(2)SO异质结是有潜力的光催化分解水材料.

关键词：第一性原理计算直接Z型异质结光催化分解水吉布斯自由能

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Influence of yttrium on the structure and magnetostriction of Fe_(83)Ga_(17) alloy

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International Journal of Minerals,Metallurgy and Materials 2012年第9期19卷 849-855页

作者： Xi-ming Xiao xue-xu Gao Ji-heng Li jian-xin Xie State Key Laboratory for Advanced Metals and Materials University of Science and Technology Beijing Beijing 100083 China School of Materials Science and Engineering University of Science and Technology Beijing Beijing 100083 China

Polycrystalline Fe83Ga17 alloy rods with various amounts of yttrium were prepared by high vacuum induction melting. It is found that yttrium addition has a significant effect on the structure and magnetostriction of Fes3Ga17 alloy. The small addition of yttrium alters the solidification character and the grain shape of Fe83Ga17 alloy, and as a result, columnar grains with the 〈100〉 preferential direction are pro- duced. Yttrium addition improves the magnetostrictive performance of the as-cast Fes3Ga17 alloy. The magnetostriction values of the as-cast alloy with 0.32at% and 0.64at% yttrium addition go up to 119×10^-6 and 137×10^-6 under 15 MPa compressive stress, respectively. The energy dispersive spectroscopy （EDS） result shows that almost all of the yttrium atoms exist in the Y2Fe17-xGax phase. A small amount of this kind of secondary phase cannot obviously increase the saturate magnetic field.

关键词： gallium alloys iron alloys yttrium magnetostfiction supercooling polycrystallirte materials