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Gli-1 siRNA induced apoptosis in Huh7 cells

World Journal of Gastroenterology 2008年第4期14卷 582-589页

作者： Xi-Lin Chen Liang-Qi Cao Miao-Rong She Qian wang Xiao-hui Huang xin-hui fu Department of Hepatobiliary Surgery the First Affiliated Hospital of Sun Yat-SenUniversity Guangzhou 510080 Guangdong Province China Department of Hematology GuangdongProvincial People's Hospital Guangzhou 510080 GuangdongProvince China Department of General SurgeryLaboratory the First Affiliated Hospital of Sun Yat-Sen UniversityGuangzhou 510080 Guangdong Province China

AIM： To investigate the effects of Gli-1 small interference RNA （siRNA） on Huh7 cells, and the change of Bcl-2 expression in Huh7 cells. METHODS： Human hepatocellular carcinoma cells Huh7 were used. Cell viability was analyzed by 3-（4, 5-Dimethylthiazol-2-yl）-2, 5-diphenyl tetrazolium bromide （MTT） assay. The expressions of Gli-1 and Bcl-2 family members were detected by RT-PCR and Western blot. Apoptosis was detected by Flow cytometry using propidium iodide, measured by Hoechst 33258 staining using Advanced Fluorescence Microscopy and caspase-3 enzymatic assay. Cell growth was analyzed after treatment with Gli-1 siRNA and 5-fluorouracil （5-fu）. RESULTS： Inhibition of Gli-1 mRNA in Huh7 cells through Gli-1 siRNA reduced cell viability. Gli-1 siRNA treatment also induced apoptosis by three criteria, increase in the sub-G1 cell cycle fraction, nuclear condensation, a morphologic change typical of apoptosis, and activation of caspase-3. Gli-1 siRNA was also able to down-regulate Bcl-2. However, Gli-1 siRNA resulted in no significant changes in Bcl-xl, Bax, Bad, and Bid. furthermore, Gli-1 siRNA increased the cytotoxic effect of 5-fu on Huh7 cell. CONCLUSION： Down-regulation of Bcl-2 plays an important role in apoptosis induced by Gli-1 siRNA in HCC cells. Combination Gli-1 siRNA with chemotherapeutic drug could represent a more promising strategy against HCC. The effects of the strategies need further investigation in vivo and may have potential clinical application.

关键词： Gli-1 transcription factor Small interferenceRNA Apoptosis Hepatocellular carcinoma 5-fluorouracil

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一种局部地面定位系统的研究与探索

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科学技术与工程 2017年第10期17卷 251-257页

作者：严新辉傅菊根安徽理工大学土木建筑学院淮南232001

针对传统定位技术定位精度低、稳定性较差,无法在室内环境定位以及定位对象局限于数字设备的缺点,提出一种依靠物体对地面产生压力进行定位的方法。该方法通过在地面下铺设经过改进的矩阵扫描电路,实时获得地面所有物体踩压位置数据,将... 详细信息

针对传统定位技术定位精度低、稳定性较差,无法在室内环境定位以及定位对象局限于数字设备的缺点,提出一种依靠物体对地面产生压力进行定位的方法。该方法通过在地面下铺设经过改进的矩阵扫描电路,实时获得地面所有物体踩压位置数据,将该数据通过互联网发送至用户智能终端实现定位。首先介绍了定位系统的原理,接着介绍局部地面定位系统中的一些参数,然后对智能终端程序进行流程分析并进行数据仿真,模拟了人在地面向各个方向行走时地面数据的变化并总结了其规律;最后,制作了相关硬件并根据仿真得出的算法进行了手机端软件的编写,完成了整个系统的开发,实现了局部地面定位系统在定位导航、安防监控、信息输入方面的功能。

关键词：定位技术扫描电路智能终端地面定位

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让观众沉浸在每一个镜头中——论艾曼努尔·卢贝兹基的镜头美学

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艺术百家 2017年第4期33卷 81-87页

作者：马立新付慧青山东师范大学传媒学院山东济南250014

艾曼努尔·卢贝兹基是享誉当代国际影坛的大师级墨西哥电影摄影师。在二十余年的艺术生涯中,他以持续的技术和艺术创新引领着当代电影摄影艺术的方向,他的每一部作品几乎都获得了各类国际专业奖项,且是唯一连续三届获得奥斯卡最佳摄影... 详细信息

艾曼努尔·卢贝兹基是享誉当代国际影坛的大师级墨西哥电影摄影师。在二十余年的艺术生涯中,他以持续的技术和艺术创新引领着当代电影摄影艺术的方向,他的每一部作品几乎都获得了各类国际专业奖项,且是唯一连续三届获得奥斯卡最佳摄影奖的艺术家;他始终恪守观众第一的美学理念,以增加观众的沉浸体验为最高追求,形成了独具特色的长镜头美学和自然摄影美学风格,在成就自身的同时,还成就了很多他的合作者。

关键词：电影艺术艾曼努尔·卢贝兹基长镜头美学自然摄影美学沉浸美学

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Vector meson's spin alignments in high energy reactions

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Science China(Physics,Mechanics & Astronomy) 2025年第1期Mechanics & Astronomy) .卷 5-20页

作者： Jin-hui Chen Zuo-Tang Liang Yu-Gang Ma xin-Li Sheng Qun Wang Key Laboratory of Nuclear Physics and Ion-beam Application (MoE) Institute of Modern PhysicsFudan University Institute of Frontier and Interdisciplinary Science Key Laboratory of Particle Physics and Particle Irradiation (MOE)Shandong University INFN-Firenze Department of Modern Physics University of Science and Technology of China School of Mechanics and Physics Anhui University of Science and Technology

The global spin alignment of vector mesons has been observed by the STAR collaboration at the Relativistic Heavy Ion Collider （RHIC） at Brookhaven National Laboratory （BNL）.It provides a unique opportunity to probe the correlation between the polarized quark and antiquark in the strongly coupled quark-gluon plasma （sQGP） produced in relativistic heavy ion collisions,opening a new window to explore the properties of s *** addition,spin alignments of vector mesons have also been observed in other high-energy particle collisions such as e+e-annihilations at high energies where hadron production is dominated by quark fragmentation *** results obtained are quite different from those obtained in heavy ion collisions where quark coalescence/combination mechanism dominates suggesting strong dependence on hadronization *** comprehensive studies in different hadronization processes are *** this article,we present a brief review of theoretical and experimental advances in the study of vector meson’s spin alignments in a variety of high-energy particle collisions,with emphasis on hadronization mechanisms.

关键词： vector meson spin alignment global polarization relativistic heavy ion collisions fragmentation

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Single-cell omics: experimental workflow, data analyses and applications

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Science China Life Sciences 2025年第1期68卷 5-102页

作者： Fengying Sun Haoyan Li Dongqing Sun Shaliu fu Lei Gu xin Shao Qinqin Wang xin Dong Bin Duan Feiyang xing Jun Wu Minmin Xiao Fangqing Zhao Jing-Dong J.Han Qi Liu Xiaohui Fan Chen Li Chenfei Wang Tieliu Shi Department of Clinical Laboratory the Affiliated Wuhu Hospital of East China Normal University (The Second People's Hospital of Wuhu City) Pharmaceutical Informatics Institute College of Pharmaceutical SciencesZhejiang University Key Laboratory of Spine and Spinal Cord Injury Repair and Regeneration (Tongji University) Ministry of EducationOrthopaedic DepartmentTongji HospitalBioinformatics DepartmentSchool of Life Sciences and TechnologyTongji University Frontier Science Center for Stem Cells School of Life Sciences and TechnologyTongji University Translational Medical Center for Stem Cell Therapy and Institute for Regenerative Medicine Shanghai East HospitalBioinformatics DepartmentSchool of Life Sciences and TechnologyTongji University Research Institute of Intelligent Computing Zhejiang Lab Shanghai Research Institute for Intelligent Autonomous Systems Center for Single-cell Omics School of Public HealthShanghai Jiao Tong University School of Medicine National Key Laboratory of Chinese Medicine Modernization Innovation Center of Yangtze River DeltaZhejiang University Center for Bioinformatics and Computational Biology Shanghai Key Laboratory of Regulatory Biologythe Institute of Biomedical Sciences and School of Life SciencesEast China Normal University Beijing Institutes of Life Science Chinese Academy of Sciences Peking-Tsinghua Center for Life Sciences Academy for Advanced Interdisciplinary StudiesCenter for Quantitative Biology (CQB)Peking University Zhejiang Key Laboratory of Precision Diagnosis and Therapy for Major Gynecological Diseases Women's HospitalZhejiang University School of Medicine Key Laboratory of Advanced Theory and Application in Statistics and Data Science-MOE School of StatisticsEast China Normal University

Cells are the fundamental units of biological systems and exhibit unique development trajectories and molecular features. Our exploration of how the genomes orchestrate the formation and maintenance of each cell, and control the cellular phenotypes of various organismsis, is both captivating and intricate. Since the inception of the first single-cell RNA technology, technologies related to single-cell sequencing have experienced rapid advancements in recent years. These technologies have expanded horizontally to include single-cell genome, epigenome,proteome, and metabolome, while vertically, they have progressed to integrate multiple omics data and incorporate additional information such as spatial sc RNA-seq and CRISPR screening. Single-cell omics represent a groundbreaking advancement in the biomedical field,offering profound insights into the understanding of complex diseases, including cancers. Here, we comprehensively summarize recent advances in single-cell omics technologies, with a specific focus on the methodology section. This overview aims to guide researchers in selecting appropriate methods for single-cell sequencing and related data analysis.

关键词： single-cell sequencing genome epigenome proteomics metabolomics multimodal spatial transcriptomics CRISPR screening

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Phylogeny and species delimitation of the C-genome diploid species in Oryza

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Journal of Systematics and Evolution 2011年第5期49卷 386-395页

作者： 1,2~Li-Li ZANG 1~xin-hui ZOU 1~fu-Min ZHANG 3,4~Ziheng YANG 1,2~Song GE 1(State Key Laboratory of Systematic and Evolutionary Botany,Institute of Botany,Chinese Academy of Sciences,Beijing 100093,China) 2(Graduate University of the Chinese Academy of Sciences,Beijing 100049,China) 3(Department of Biology,University College London,London WC1E 6BT,United Kingdom) 4(Center for Computational and Evolutionary Biology,Institute of Zoology,Chinese Academy of Sciences,Beijing 100101,China) (State Key Laboratory of Systematic and Evolutionary Botany Institute of Botany Chinese Academy of Sciences Beijing 100093 China) (Graduate University of the Chinese Academy of Sciences Beijing 100049 China) (Department of Biology University College London London WC1E 6BT United Kingdom) (Center for Computational and Evolutionary Biology Institute of Zoology Chinese Academy of Sciences Beijing 100101 China)

The diploid Oryza species with C-genome type possesses abundant genes useful for rice improvement and provides parental donors of many tetraploid species with the C-genome(BBCC,CCDD).Despite extensive studies,the phylogenetic relationship among the C-genome species and the taxonomic status of some taxa remain *** this study,we reconstructed the phylogeny of three diploid species with C-genome(Oryza officinalis,***,and ***) based on sequences of 68 nuclear single-copy *** obtained a fully resolved phylogenetic tree,clearly indicating the sister relationship of *** and ***,with *** being the more divergent *** phylogenies of the C-genome species found in previous studies might result from lineage sorting,introgression/hybridization and limited number of genetic markers *** further applied a recently developed Bayesian species delimitation method to investigate the species status of the Sri Lankan and African *** of two datasets(68 genes with a single sample,and 10 genes with multiple samples) support the distinct species status of the Sri Lankan and African *** addition,we evaluated the impact of the number of sampled individuals and loci on species *** simulation suggests that sampling multiple individuals is critically important for species delimitation,particularly for closely related species.

关键词： Bayesian species delimitation Oryza phylogeny taxonomy.

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The interaction between KIF21A and KANK1 regulates dendritic morphology and synapse plasticity in neurons

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Neural Regeneration Research 2025年第1期20卷 209-223页

作者： Shi-Yan Sun Lingyun Nie Jing Zhang Xue Fang Hongmei Luo Chuanhai fu Zhiyi Wei Ai-hui Tang Hefei National Research Center for Physical Sciences at the Microscale CAS Key Laboratory of Brain Function and DiseaseMinistry of Education Key Laboratory for Membrane-less Organelles and Cellular DynamicsDivision of Life Sciences and MedicineUniversity of Science and Technology of ChinaHefeiAnhui ProvinceChina Institute of Artificial Intelligence Hefei Comprehensive National Science CenterHefeiAnhui ProvinceChina CAS Center for Excellence in Molecular Cell Sciences Division of Life Sciences and MedicineUniversity of Science and Technology of ChinaHefeiAnhui ProvinceChina Department of Neurobiology School of Life SciencesSouthern University of Science and TechnologyShenzhenGuangdong ProvinceChina Brain Research Center Southern University of Science and TechnologyShenzhenGuangdong ProvinceChina

Morphological alterations in dendritic spines have been linked to changes in functional communication between neurons that affect learning and ***-4 KIF21A helps organize the microtubule-actin network at the cell cortex by interacting with KANK1;however,whether KIF21A modulates dendritic structure and function in neurons remains *** this study,we found that KIF21A was distributed in a subset of dendritic spines,and that these KIF21A-positive spines were larger and more structurally plastic than KIF21A-negative ***,the interaction between KIF21A and KANK1 was found to be critical for dendritic spine morphogenesis and synaptic *** of either KIF21A or KANK1 inhibited dendritic spine morphogenesis and dendritic branching,and these deficits were fully rescued by coexpressing full-length KIF21A or KANK1,but not by proteins with mutations disrupting direct binding between KIF21A and KANK1 or binding between KANK1 and *** down KIF21A in the hippocampus of rats inhibited the amplitudes of long-term potentiation induced by high-frequency stimulation and negatively impacted the animals’cognitive *** together,our findings demonstrate the function of KIF21A in modulating spine morphology and provide insight into its role in synaptic function.

关键词： actin cytoskeleton dendrite KANK1 KIF21A microtubule spine morphology spine synaptic plasticity talin1

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Pancreatic stent improves the success rate of needle-knife papillotomy in patients with difficult biliary cannulation

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World Journal of Gastroenterology 2025年第1期31卷 58-67页

作者： Mu-Hsien Lee Cheng-hui Lin Chi-Huan Wu Yung-Kuan Tsou Kai-Feng Sung Sheng-fu Wang Nai-Jen Liu Department of Gastroenterology and Hepatology Chang Gung Memorial Hospital and Chang Gung University College of MedicineTaoyuan 33305Taiwan School of Medicine Chang Gung UniversityTaoyuan 33305Taiwan

BACKGROUND Needle-knife precut papillotomy(NKP)is typically performed ***,it remains unclear whether pancreatic stent(PS)placement can improve the outcomes of *** To explore whether PS placement improves the success rate of NKP in patients with difficult biliary *** This single-center retrospective study included 190 patients who underwent NKP between January 2017 and December 2021 after failed conventional biliary *** cases with incidental pancreatic duct cannulation during conventional biliary cannulation,the decision for pre-NKP PS placement was made at the endoscopist's *** primary outcome was the difference in the NKP success rate between patients with and without PS placement;the secondary outcome was the adverse event *** Among the 190 participants,82 received pre-NKP PS(PS-NKP group)whereas 108 did not[freehand or freehand NKP(FH-NKP)group].Post-NKP selective biliary cannulation was successful in 167(87.9%)patients,and the PS-NKP had a significantly higher success rate than the FH-NKP group(93.9%vs 83.3%,P=0.027).The overall adverse event rates were 7.3%and 11.1%in the PS-NKP and FH-NKP groups,respectively(P=0.493).A periampullary diverticulum(PAD)and significant intraoperative bleeding during NKP were independently associated with NKP failure;however,a pre-NKP PS was the only predictor of NKP *** the 44 participants with PADs,the PS-NKP group had a non-significantly higher NKP success rate than the FH-NKP group(87.5%and 65%,respectively;P=0.076).CONCLUSION PS significantly improved the success rate of NKP in patients with difficult biliary cannulation.

关键词： Difficult biliary cannulation Endoscopic retrograde cholangiopancreatography Needle-knife papillotomy Pancreatic stent Selective biliary cannulation

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基于XML的仿真想定标记语言SSML

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系统仿真学报 2004年第9期16卷 1928-1930,2025页

作者：陈欣胡晓惠付勇傅妤华北京系统工程研究所北京100101

想定管理是仿真系统中的重要组成部分,想定管理的目标是实现想定与仿真相分离的处理机制,保持想定数据的独立性。本文提出的基于XML的仿真想定标记语言SSML,利用XML的Schema定义想定数据的约束规则并进行语法检查。通过SSML所提供的中... 详细信息

想定管理是仿真系统中的重要组成部分,想定管理的目标是实现想定与仿真相分离的处理机制,保持想定数据的独立性。本文提出的基于XML的仿真想定标记语言SSML,利用XML的Schema定义想定数据的约束规则并进行语法检查。通过SSML所提供的中间访问层实现想定处理与想定数据库存储模式的分离。利用XML所提供的Xquery标准和Xpath标准实现想定数据的可伸缩性、动态裁减和重组。

关键词：想定管理可扩展的标记语言(XML) 仿真想定标记语言(SSML) 高层体系结构(HLA)

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Staged dendrite suppression for high safe and stable lithium-sulfur batteries

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Journal of Energy Chemistry 2025年第1期100卷 674-683页

作者： Jun Jiang Zhenjie Lu Yanwen Ding Shujun Liu Zhijie Qi Tian Tang Yunfan Zhang Zhiyuan Ma Jingwen Sun Liang Xue Wenyao Zhang Pan Xiong xin Wang Junwu Zhu Yongsheng fu Key Laboratory for Soft Chemistry and functional Materials of Ministry of Education Nanjing University of Science and Technology Jiangsu Pylon Battery Co.Ltd

The unavoidable dendrite growth and shuttle effect have long been stranglehold challenges limiting the safety and practicality of lithium-sulfur ***,we propose a dual-action strategy to address the lithium dendrite issue in stages by constructing a multifunctional surface-negatively-charged nanodiamond layer with high ductility and robust puncture resistance on polypropylene （PP） *** uniformly loaded compact negative layer can not only significantly enhance electron transmission efficiency and promote uniform lithium deposition,but also reduce the formation of dendrite during early deposition *** importantly,under the strong puncture stress encountered during the deterioration of lithium dendrite growth under limiting current,the high ductility and robust puncture resistance（145.88 MPa） of as-obtained nanodiamond layer can effectively prevent short circuits caused by unavoidable lithium *** Li||Li symmetrical cells assembled with nanodiamond layer modified PP demonstrated a stable cycle of over 1000 h at 2 mA cm-2with a polarization voltage of only 29.3 ***,the negative charged layer serves as a physical barrier blocking lithium polysulfide ions,effectively mitigating capacity *** improved cells achieved a capacity decay of only 0.042%per cycle after 700 cycles at 3 C,demonstrating effective suppression of dendrite growth and capacity attenuation,showing promising prospect.

关键词： Lithium-sulfur batteries Staged dendrite suppression Shuttle effect Surface-negatively-charged nanodiamond

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