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Deep learning identification of novel autophagic protein-protein interactions and experimental validation of Beclin 2-Ubiquilin 1 axis in triple-negative breast cancer

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Oncology Research 2025年第1期33卷 67-81页

作者： XIANG LI WENKE JIN LIFENG WU huan WANG xin XIE WEI huanG BO LIU State Key Laboratory of Southwestern Chinese Medicine Resources School of Pharmacy and College of Medical TechnologyChengdu University of Traditional Chinese MedicineChengdu611137China Department of Biotherapy Cancer Center and State Key Laboratory of BiotherapyWest China HospitalSichuan UniversityChengdu610041China

Background:Triple-negative breast cancer(TNBC),characterized by its lack of traditional hormone receptors and HER2,presents a significant challenge in oncology due to its poor response to conventional *** is an important process for maintaining cellular homeostasis,and there are currently autophagy biomarkers that play an effective role in the clinical treatment of *** contrast to targeting protein activity,intervention with proteinprotein interaction(PPI)can avoid unrelated crosstalk and regulate the autophagy process with minimal interference ***:Here,we employed Naive Bayes,Decision Tree,and k-Nearest Neighbors to elucidate the complex PPI network associated with autophagy in TNBC,aiming to uncover novel therapeutic ***,the candidate proteins interacting with Beclin 2 were initially screened in MDA-MB-231 cells using Beclin 2 as bait protein by immunoprecipitation-mass spectrometry assay,and the interaction relationship was verified by molecular docking and CO-IP experiments after *** formation,cellular immunofluorescence,cell scratch and 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT)tests were used to predict the clinical therapeutic effects of manipulating candidate ***:By developing three PPI classification models and analyzing over 13,000 datasets,we identified 3733 previously unknown autophagy-related *** network analysis revealed the central role of Beclin 2 in autophagy regulation,uncovering its interactions with 39 newly identified ***,the CO-IP studies identified the substantial interaction between Beclin 2 and Ubiquilin 1,which was anticipated by our model and discovered in immunoprecipitation-mass spectrometry assay ***,in vitro investigations showed that overexpressing Beclin 2 increased Ubiquilin 1,promoted autophagy-dependent cell death,and inhibited proliferation and metastasis in MDA-MB-231 ***:This study not

关键词： Triple-negative breast cancer(TNBC) Autophagy Protein-protein interactions(PPI) Artificial intelligence(AI) Beclin 2 Ubiquilin 1

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空调列车运行时环境温度变化规律的研究

铁道学报 2003年第3期25卷 31-34页

作者：陈焕新杨培志中南大学土木建筑学院湖南长沙410075

传统的列车空调负荷计算方法是将列车作为静物处理,它不能反映外部环境温度随时间和空间的不同而变化这一特点。本文综合考虑了列车运行时外部环境温度的动态变化,计算得到车厢内壁面的温度波,反映了列车运行时环境温度变化规律的影响,... 详细信息

传统的列车空调负荷计算方法是将列车作为静物处理,它不能反映外部环境温度随时间和空间的不同而变化这一特点。本文综合考虑了列车运行时外部环境温度的动态变化,计算得到车厢内壁面的温度波,反映了列车运行时环境温度变化规律的影响,为今后列车空调动态负荷计算及列车空调机组实时运行调节提供了理论依据。

关键词：温度空调列车空调负荷

Mechanism of action of Linggui Zhugan Decoction in treating non-alcoholic fatty liver disease using untargeted metabolomics approach

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Traditional Medicine Research 2025年第2期10卷 65-76页

作者： huan Pei Ren-Lin Li xin-Ran Song Qian-Qian Wan Yu-Ming Wang Han-Zhou Li Wei-Quan Xu Jia-Bao Liao Wei-Bo Wen Jing Miao huan-Tian Cui School of Basic Medicine Yunnan University of Chinese MedicineKunming 650599China School Clinical Medical College Yunnan University of Chinese MedicineKunming 650599China First School of Clinical Medicine Yunnan University of Chinese MedicineKunming 650599China School of Integrative Chinese and Western Medicine Tianjin University of Traditional Chinese MedicineTianjin 301617China Department of Pharmacy Tianjin Second People's HospitalTianjin 300192China

Background:Non-alcoholic fatty liver disease(NAFLD)is a liver disorder characterized by the accumulation and degeneration of fat in the liver cells,a condition that may further deteriorate and lead to cirrhosis and liver *** studies showed that metabolic dysfunction can promote NAFLD *** Zhugan Decoction(LGZGD)has therapeutic effects on *** mechanism of LGZGD still remains *** study was to examine the impact of LGZGD on the metabolic processes involved in the development of ***:A mice model of NAFLD was treated with *** therapeutic potential of LGZGD was evaluated by assessing the activity of transaminases,lipids levels of blood,and pathological changes in the liver of the mice model of ***,this study also evaluated the influence of LGZGD on liver inflammation and oxidative ***:The results of untargeted metabolomics analysis showed that LGZGD reduced the disordered lipid metabolism in NAFLD *** improved the oxidative stress and also reduced the levels of pro-inflammatory cytokines in the *** metabolomics analysis of liver samples revealed that LGZGD treatment improved metabolic disorders,including alanine,aspartate,glutamate,glycerophospholipid metabolism,and citrate *** RT-qPCR and Western blot results showed that LGZGD could regulate the expression of key enzymes in the metabolic pathway of the citrate cycle,including ATP-citrate lyase(ACLY),alanine-glyoxylate aminotransferase-2(AGXT2),phosphatidylethanolamine N-methyltransferase(PEMT),and succinate dehydrogenase(SDH).Conclusion:We found that LGZGD can treat NAFLD by reducing inflammatory responses,inhibiting oxidative stress,regulating alanine,aspartate,glutamate,and glycerophospholipid metabolism,and citrate cycle pathways.

关键词： NAFLD LGZGD untargeted metabolomics glycerophospholipid metabolism citrate cycle

Nanomedicine-boosting icaritin-based immunotherapy of advanced hepatocellular carcinoma

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Military Medical Research 2023年第3期10卷 403-414页

作者： Yi Lu Yue Gao huan yang Yong Hu xin Li Department of Polymeric Materials School of Materials Science and EngineeringTongji UniversityShanghai 201804China Institute of Biotechnology RWTH Aachen University52074 AachenGermany DWI-Leibniz-Institute for Interactive Materials e.V. 52056 AachenGermany College of Chemistry Chemical Engineering and BiotechnologyDonghua UniversityShanghai 201620China School of Pharmacy Jiangsu UniversityZhenjiang 212013JiangsuChina Institute of Technical and Macromolecular Chemistry RWTH Aachen University52074 AachenGermany

Traditional treatments for advanced hepatocellular carcinoma(HCC),such as surgical resection,transplantation,radiofrequency ablation,and chemotherapy are unsatisfactory,and therefore the exploration of powerful therapeutic strategies is urgently *** has emerged as a promising strategy for advanced HCC treatment due to its minimal side effects and long-lasting therapeutic memory *** studies have demonstrated that icaritin could serve as an immunomodulator for effective immunotherapy of advanced ***,in 2022,icaritin soft capsules were approved by the National Medical Products Administration(NMPA)of China for the immunotherapy of advanced ***,the therapeutic efficacy of icaritin in clinical practice is impaired by its poor bioavailability and unfavorable in vivo delivery ***,functionalized drug delivery systems including stimuli-responsive nanocarriers,cell membrane-coated nanocarriers,and living cell-nanocarrier systems have been designed to overcome the shortcomings of drugs,including the low bioavailability and limited delivery efficiency as well as side *** together,the development of icaritin-based nanomedicines is expected to further improve the immunotherapy of advanced ***,we compared the different preparation methods for icaritin,interpreted the HCC immune microenvironment and the mechanisms underlying icaritin for treatment of advanced HCC,and discussed both the design of icaritin-based nanomedicines with high icaritin loading and the latest progress in icaritinbased nanomedicines for advanced HCC ***,the prospects to promote further clinical translation of icaritin-based nanomedicines for the immunotherapy of advanced HCC were proposed.

关键词： Icaritin Nanomedicine Advanced hepatocellular carcinoma Immunotherapy Clinical translation

Effect of Ni/(La+Mg) Ratio on Structure and Electrochemical Performance of La-Mg-Ni Alloy System

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Journal of Iron and Steel Research International 2009年第3期16卷 83-88页

作者： DONG Xiao-ping yang Li-ying ZHANG yang-huan WANG xin-lin College of Quality and Technical Supervision Hebei University Baoding 071002 Hebei China Department ofFunctional Material Research China Iron and Steel Research Institute Group Beijing 100081 China

As the alloy with the most suitable Ni/（La＋Mg） ratio has higher capacity and good cycle stability,the effects of Ni/（La＋Mg） ratios on the electrochemical performances of the La0.80Mg0.20Nix （x=3.5 to 5.0） alloys have been investigated to find the most suitable Ni/（La＋Mg） ratio. The results of XRD and SEM observations show that the phase composition of the alloys varies with different Ni/（La＋Mg） ratios. When Ni/（La＋Mg） is not more than 4.25,all the alloys contain LaNi5 and （La,Mg）2Ni7 phases,in addition,the LaMg and （La,Mg）Ni3 phases exist in the x=3.5 and 3.75 alloys,respectively. The LaMg2Ni9 phase exists in the x=4.25 alloy. There are the LaNi5 and LaMg2Ni9 phases in the x=4.5,4.75,and 5.0 alloys. The phase abundance and cell volume change with different Ni content. When the Ni/（La＋Mg） ratio is not more than 4.25,the alloys possess excellent activation capability,however,the activation capabilities of the alloys decrease with a further increase in the Ni/（La＋Mg） ratio. With increasing the Ni/（La＋Mg） ratio,the maximum discharge capacities,the medium voltages,and the cycle stabilities of the alloys first increase and then decrease. When the Ni/（La＋Mg） ratio is 3.75,the corresponding alloy has the maximum discharge capacity among all the alloys. However,the cycle stability of the Ni/（La＋Mg）=4.0 alloy is better than that of the others.

关键词： Ni/(La+Mg) ratio La-Mg-Ni alloy system structure characteristic electrochemical performance

Effects of high temperature during grain filling on physicochemical properties of waxy maize starch

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Journal of Integrative Agriculture 2016年第2期15卷 309-316页

作者： LU Da-lei yang huan SHEN xin LU Wei-ping Jiangsu Key Laboratory of Crop Genetics and Physiology/Co-Innovation Center for Modern Production Technology of Grain Crops Yangzhou University

Understanding the waxy maize starch physicochemical properties response to heat stress during grain filling could improve starch *** effects of heat stress during early（1-15 days after pollination,DAP） and late（16-30 DAP） grain filling stages on the starch physicochemical properties of four waxy maize varieties were *** only increased in Suyunuo 5 after exposure to high temperature at late grain filling *** effects of heat stress on digestibility and swelling power were dependent on varieties and ***,swelling power was increased by heat stress at early grain development stage and digestibility was increased by high temperature at late grain filling stage,*** results of correlation analysis indicated the starch with large granule size could swell well and easy ***,trough,final,and breakdown viscosities in response to heat stress were dependent on stages and *** general,peak,trough and final viscosities were decreased and increased by heat stress at early grain formation and late grain filling stages,respectively;whereas the breakdown and setback viscosities were similar among the three *** stress increased the gelatinization temperatures and retrogradation *** range decreased under heat stress at 1-15 DAP but remained constant under heat stress at 16-30 DAP in all *** starch exposed to high temperature at 16-30 DAP presented higher digestibility and peak viscosity and lower retrogradation percentage than those at 1-15 ***,heat stress at early grain formation stage severely affects the physicochemical properties of starch.

关键词： waxy maize heat stress digestibility pasting gelatinization retrogradation

Utilization-Aware Data Variable Allocation on NVM- Based SPM in Real-Time Embedded Systems

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Journal of Electronic Science and Technology 2021年第2期19卷 163-172页

作者： Jin-Yu Zhan Yi-xin Li Wei Jiang Jun-huan yang School of Information and Software Engineering University of Electronic Science and Technology of ChinaChengdu 610054 State Key Lab of Mathematical Engineering and Advanced Computing Wuxi 214000

With the development of the nonvolatile memory(NVM),using NVM in the design of the cache and scratchpad memory(SPM)has been *** paper presents a data variable allocation(DVA)algorithm based on the genetic algorithm for NVM-based SPM to prolong the *** lifetime can be formulated indirectly as the write counts on each SPM *** the differences between global variables and stack variables,our optimization model has three *** constraints of the central processing unit(CPU)utilization and size are used for all variables,while no-overlay constraint is only used for stack *** satisfy the constraints of the optimization model,we use the greedy strategy to generate the initial population which can determine whether data variables are allocated to SPM and distribute them evenly on SPM ***,we use the Mälardalen worst case executive time(WCET)benchmark to evaluate our *** experimental results show that the DVA algorithm can not only obtain close-to-optimal solutions,but also prolong the lifetime by 9.17% on average compared with SRAM-based SPM.

关键词： Data variables allocation genetic algorithm nonvolatile momory(NVM) scratchpad memory(SPM)

“To live a meaningful life,be yourself and make yourself”:Haoyan Jen—a pioneer of China’s environmental microbiology

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Protein & Cell 2021年第2期12卷 81-83页

作者： xin Zhang huan Liu Institute of Biophysics Chinese Academy of SciencesBeijing100101China Wuhan Institute of Virology Chinese Academy of SciencesWuhan430071China Chinese Center for Disease Control and Prevention Beijing102206China State Key Laboratory of Virology Wuhan430072China

There is a man who improves our living and working environment by turning pesticide residues in the field into nutrients for the crops,turning such wastes as paper and sawdust into sugar,which is then converted into liquid alcohol fuel,or purifying malodorous and toxic industrial wastewater into clean and non-toxic *** is *** Jen(简浩然),a pioneer of China’s environmental microbiology.

关键词： wastewater yourself turning

Hydrogen storage thermodynamics and kinetics of as-cast Ce-Mg-Ni-based alloy

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Journal of Iron and Steel Research International 2024年第3期31卷 752-766页

作者： Yan Qi xin Zhang Jun Li Dong-liang Zhao Shi-hai Guo yang-huan Zhang Department of Functional Material Research Central Iron and Steel Research InstituteBeijing 100081China China Rare Earth New Material(Weishan)Co..Ltd. Jining 277600ShandongChina Collaborative Innovation Center of Integrated Exploitation of Bayan Obo Multi-Metal Resources Inner Mongolia University of Science and TechnologyBaotou 014010Inner MongoliaChina

The reaction kinetics of alloys based on magnesium are known to be greatly improved by the partial substitution of Mg with rare earths and transition metals,particularly *** enhanced superficial hydrogen dissociation rate,the weakened Mg-H bond and the lower activation energy following element replacement are thought to be related to the better *** experimental alloys Ce5Mg_(95-x)Ni_(x)(x=5,10,15)were smelted by the vacuum induction *** phase transformation and structural evolution of experimental alloys before and after reaction with hydrogen were char-acterized by X-ray diffraction,scanning electron microscopy and transmission electron *** cast specimens contain CeMg_(12),Mg and Mg_(2)Ni phases,and the increase in Ni content results in an obvious growth of Mg_(2)Ni *** isothermal and non-isothermal hydrogenation and dehydrogenation kinetics of the experimental specimens were investi-gated using the Sievert apparatus,differential scanning calorimetry and thermal gravimetric *** activation energy may be calculated using the Arrhenius and Kissinger *** experimental alloys have been shown to have good activation properties,with a reversible hydriding and dehydriding capacities of around 5.0 wt.%in the first *** initial dehydrogenation temperature of MgH_(2) decreases from 557.5 to 537.7 K with changing Ni content from 5 to 15 at.%.The dehydrogenation activation energy also reduces from 77.09 to 62.96 kJ/mol,which explains the improved hydrogen storage performance caused by Ni *** can be shown that the impact of Ni on the decomposition enthalpy of MgH_(2) is quite modest,with the absolute enthalpy(ΔHr)only decreasing from 78.48 to 76.15 kJ/mol.

关键词： Mg-based alloy Ni content Hydrogen storage kinetics Thermodynamic property Activation energy