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Predicting disease progression in cirrhotic patients after transjugular intrahepatic portosystemic shunt implantation:A sexstratified analysis

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World Journal of Gastroenterology 2023年第42期29卷 5768-5780页

作者： Qian Zhang Li Long Hong-Lin Zhu Hong Peng xin-hua luo Kang-Shun Zhu Rong-Pin Wang Department of Radiology Guizhou Provincial People’s HospitalGuiyang 550002Guizhou ProvinceChina The Second Affiliated Hospital of Guangzhou Medical University Guangzhou 510260Guangdong ProvinceChina Department of Infectious Diseases Guizhou Provincial People’s HospitalGuiyang 550002Guizhou ProvinceChina Department of Interventional Radiology Guizhou Provincial People’s HospitalGuiyang 550002Guizhou ProvinceChina Department of Interventional Radiology The Second Affiliated Hospital of Guangzhou Medical UniversityGuangzhou 510260Guangdong ProvinceChina

BACKGROUND Transjugular intrahepatic portosystemic shunt(TIPS)has been extensively used to treat portal hypertension-associated complications,including *** prediction of post-TIPS prognosis is important for cirrhotic patients,as more aggressive liver transplantation is needed when the post-TIPS prognosis is *** To construct a nutrition-based model that could predict the disease progression of cirrhotic patients after TIPS implantation in a sex-dependent *** This study retrospectively recruited cirrhotic patients undergoing TIPS implantation for *** quality was assessed by measuring the skeletal muscle index(SMI)by computed *** Cox proportional hazard models were utilized to determine the association between SMI and disease progression in cirrhotic patients after TIPS *** This study eventually included 186 cirrhotic patients receiving TIPS who were followed up for 30.5±18.8 *** male patients,the 30-mo survival rate was significantly lower and the probability of progressive events was higher(3.257-fold)in the low-level SMI group than in the high-level SMI *** to the multivariate Cox analysis of male patients,SMI<32.8 was an independent risk factor for long-term adverse outcomes after TIPS implantation.A model was constructed,which involved creatinine,plasma ammonia,SMI,and acute-on-chronic liver failure and hepatic encephalopathy occurring within half a year after *** model had an area under the receiver operating characteristic curve of 0.852,sensitivity of 0.926,and specificity of *** to the results of the DeLong test,this model outperformed other models(Child-Turcotte-Pugh,Model for End-Stage Liver Disease,and Freiburg index of post-TIPS survival)(P<0.05).CONCLUSION SMI is strongly associated with poor long-term outcomes in male patients with cirrhosis who underwent TIPS implantation.

关键词： Transjugular intrahepatic portosystemic shunt Skeletal muscle index Nutrition Cirrhosis Sex stratified Prognosis

Chloride intracellular channel 1 regulates colon cancer cell migration and invasion through ROS/ERK pathway

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World Journal of Gastroenterology 2014年第8期20卷 2071-2078页

作者： Pan Wang Yun Zeng Tao Liu Chao Zhang Pei-Wu Yu Ying-Xue Hao hua-xin luo Gang Liu Department of General Surgery Affiliated Hospital of North Sichuan Medical College Department of General Surgery and Minimally Invasive Gastrointestinal Surgery Southwest HospitalThird Military Medical University Sichuan Key Laboratory of Medical Imaging North Sichuan Medical College Center for Molecular Imaging and Translational Medicine School of Public HealthXiamen University

AIM: To investigate the mechanisms of chloride intracellular channel 1 (CLIC1) in the metastasis of colon cancer under hypoxia-reoxygenation (H-R) conditions.

关键词： Colon cancer Intracellular chloride channel 1 Hypoxia-reoxygenation Reactive oxygen species Extracellular signal-regulated kinase Cancer invasion

Silent information regulator sirtuin 1 ameliorates acute liver failure via the p53/glutathione peroxidase 4/gasdermin D axis

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World Journal of Gastroenterology 2024年第11期30卷 1588-1608页

作者： xing-Nian Zhou Quan Zhang Hong Peng Yu-Jie Qin Yu-Hong Liu Lu Wang Ming-Liang Cheng xin-hua luo Hong Li Department of Infectious Diseases The Affiliated Hospital of Guizhou Medical UniversityGuizhou Medical UniversityGuiyang 550004Guizhou ProvinceChina Department of Infectious Diseases Guizhou Provincial People's HospitalGuiyang 550001Guizhou ProvinceChina

BACKGROUND Acute liver failure(ALF)has a high mortality with widespread hepatocyte death involving ferroptosis and *** silent information regulator sirtuin 1(SIRT1)-mediated deacetylation affects multiple biological processes,including cellular senescence,apoptosis,sugar and lipid metabolism,oxidative stress,and *** To investigate the association between ferroptosis and pyroptosis and the upstream regulatory *** This study included 30 patients with ALF and 30 healthy individuals who underwent serum alanine aminotransferase(ALT)and aspartate aminotransferase(AST)testing.C57BL/6 mice were also intraperitoneally pretreated with SIRT1,p53,or glutathione peroxidase 4(GPX4)inducers and inhibitors and injected with lipopolysaccharide(LPS)/D-galactosamine(D-GalN)to induce *** D(GSDMD)^(-/-)mice were used as an experimental *** changes in liver tissue were monitored by hematoxylin and eosin ***,AST,glutathione,reactive oxygen species,and iron levels were measured using commercial ***-and pyroptosis-related protein and mRNA expression was detected by western blot and quantitative real-time polymerase chain ***1,p53,and GSDMD were assessed by immunofluorescence *** Serum AST and ALT levels were elevated in patients with ***1,solute carrier family 7a member 11(SLC7A11),and GPX4 protein expression was decreased and acetylated p5,p53,GSDMD,and acyl-CoA synthetase long-chain family member 4(ACSL4)protein levels were elevated in human ALF liver *** the p53 and ferroptosis inhibitor-treated and GSDMD^(-/-)groups,serum interleukin(IL)-1β,tumour necrosis factor alpha,IL-6,IL-2 and C-C motif ligand 2 levels were decreased and hepatic impairment was *** mice with GSDMD knockout,p53 was reduced,GPX4 was increased,and ferroptotic events(depletion of SLC7A11,elevation of ACSL4,and iron accumulation)were *** vitro,knockdown of p53 and overexpression of GPX

关键词： Silent information regulator sirtuin 1 Ferroptosis Pyroptosis p53/glutathione peroxidase 4/gasdermin D Acute liver failure

Assessing recent recurrence after hepatectomy for hepatitis Brelated hepatocellular carcinoma by a predictive model based on sarcopenia

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World Journal of Gastroenterology 2024年第12期30卷 1727-1738页

作者： Hong Peng Si-Yi Lei Wei Fan Yu Dai Yi Zhang Gen Chen Ting-Ting Xiong Tian-Zhao Liu Yue huang Xiao-Feng Wang Jin-Hui Xu xin-hua luo Department of Infectious Diseases Guizhou Provincial People’s HospitalGuiyang 550002Guizhou ProvinceChina Department of Hepatobiliary Surgery Guizhou Provincial People’s HospitalGuiyang 550002Guizhou ProvinceChina

BACKGROUND Sarcopenia may be associated with hepatocellular carcinoma(HCC)following *** traditional single clinical variables are still insufficient to predict *** still lack effective prediction models for recent recurrence(time to recurrence5 cm(HR=2.222,95%CI:1.285-3.842,P<0.05),and hepatitis B virus DNA level≥2000 IU/mL(HR=2.1,95%CI:1.407-3.135,P<0.05)were independent risk factors associated with postoperative recurrence of *** on the sarcopenia to assess the RFS model of hepatectomy with hepatitis B-related liver cancer disease(SAMD)was established combined with other the above risk *** area under the curve of the SAMD model was 0.782(95%CI:0.705-0.858)in the training cohort(sensitivity 81%,specificity 63%)and 0.773(95%CI:0.707-0.838)in the validation ***,a SAMD score≥110 was better to distinguish the high-risk group of postoperative recurrence of *** Sarcopenia is associated with recent recurrence after hepatectomy for hepatitis B-rel

关键词： Alpha-fetoprotein Hepatitis B virus Hepatectomy Hepatocellular carcinoma Nomogram Predictive models Recurrence Recurrence-free survival Risk factors Sarcopenia

Effect of Danshao huaxian capsule on expression of matrix metalloproteinase-1 and tissue inhibitor of metalloproteinase-1 in fibrotic liver of rats

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World Journal of Gastroenterology 2005年第32期11卷 4953-4956页

作者： Qin Yang Ru-Jia Xie Xiao-Xia Geng xin-hua luo Bing Han Ming-Liang Cheng Department of Pathophysiology Guiyang Medical College Guiyang 550004 Guizhou Province China Department of Infectious Diseases Affiliated Hospital of Guiyang Medical College Guiyang 550004 Guizhou Province China Department of Infectious Diseases People's Hospital of Guizhou Province Guiyang 550004 Guizhou Province China

AIM： To investigate the effects of Danshao huaxian （DSHX） capsules, a preparation of traditional Chinese medicine, on the expression of matrix metalloproteinase-1 （MMP-1）, and tissue inhibitor of metalloproteinase-1 （TIMP-1） in the fibrous livers of rats. METHODS： Eighty male Wistar rats were randomly divided into normal control group （group A）, CCl4-induced hepatic fibrosis group （group B）, non-DSHX-treated group （group C）, low dose-treated group （group D）, and high dose-treated group （group E）. Fibrous liver models in rats were induced by subcutaneous injection of CCI4, oral administration of alcohol and high-lipid/low-protein diet for 8 wk. After the models were established, the rats in groups D and E were orally given a low dose （0.5 g/kg） and a high dose （1.0 g/kg） of DSHX daily for 8 wk, respectively. Then, the liver indexes, serum hyaluronic acid （HA） and alanine aminotransferase （ALT） were examined. The degree of hepatic fibrosis was evaluated by optical microscopy. Hydroxyproline （Hyp） in the urine was determined, and the expression of MMP-1 and TIMP-1 was detected by immunohistochemical techniques. RESULTS： In groups D and E, the liver indexes, levels of serum HA and ALT reduced and development of hepatic fibrosis weakened significantly. The urinary Hyp and expression of MMP-1 in the liver tissues elevated, but the expression of TIMP-1 decreased obviously, as compared to groups B and ***： DSHX enhances the expression of MMP-1 but decreases that of TIMP-1 in liver tissues of CCI4-induced hepatic fibrotic rats, which may result in its elevated activity that contributes to fighting against hepatic fibrosis.

关键词： Danshao huaxian capsule Hepatic fibrosis MMP-1 TIMP-1 Collagen

Effects of Dan-Shao-hua-Xian on the expression of collagen type I and III in rats with hepatic fibrosis

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Hepatobiliary & Pancreatic Diseases International 2004年第4期3卷 558-563页

作者： Xiao-Xia Geng, Qin Yang, Ru-Jia Xie, xin-hua luo, Cheng-Xiu Li and Ming-Liang Cheng Guizhou, China Department of Infectious Diseases, Affiliated Hospital of Guiyang Medical College Department of Pathophysiology, Guiyang Medical College Depart- ment of Infectious Diseases, People’ s Hospital of Guizhou Province and Department of Pharmacology, Guiyang Medical College Department of Infectious Diseases Affiliated Hospital of Guiyang Medical College Guizhou 550004 China.

BACKGROUND: Hepatic fibrosis is the common patholo gical change in various chronic liver diseases,and its major cause is the imbalance between the production and degra dation of the extracellular matrix, which is mainly com posed of collagens. Dan-Shao-hua-Xian ( DSHX) cap sule, a traditional Chinese herbal compound, has shown marked preventive effects on hepatic fibrosis in rats in our previous studies. The present study was designed to further investigate its therapeutic actions on hepatic fibrosis in rats and its possible mechanisms. METHODS: Eighty male Wistar rats were randomly divid- ed into normal control group, hepatic fibrosis group, non DSHX-treated group, low-dose-treated group, and high dose-treated group. The rat models of hepatic fibrosis were established by subcutaneous injecton of CC14 , drinking al- cohol , giving diet of hyperliprosis and hypoprotein for 8 weeks. The two DSHX-treated groups were treated respec- tively with low dose (0.5 g/kg) and high dose (1.0 g/kg) of DSHX capsule p. o. everyday for 8 weeks. At the end of the experiment, liver indexes were calculated in each group in addition to the levels of the serum hyaluronic acid and alanine aminotransferase. Their degree of hepatic fibrosis and urinary excretion of hydroxyproline and expression of collagen , were detected. RESULTS: Comparison of the indexes of the hepatic fibro- sis group and non-DSHX-treated group revealed that the liver indexes, levels of serum hyaluronic acid and alanine aminotransferase, and stage of hepatic fibrosis were all sig- nificantly reduced in the two DSHX treated groups. The urinary excretion of hydroxyproline was increased and the expression of collagen I and HI in liver tissue was lessened. These alterations were more obviously observed in the high-dose-treated group. CONCLUSION: DSHX capsule has certain therapeutic effect on hepatic fibrosis in rats.

关键词： liver fibrosis collagen hyaluronic acid hydroxyproline traditional Chinese herbs

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应用基因芯片对人肝纤维化相关基因的筛查

世界华人消化杂志 2008年第14期16卷 1525-1529页

作者：罗新华杨勤张权程明亮贵阳医学院附属医院感染科贵州省贵阳市550004 贵阳医学院病理生理学教研室贵州省贵阳市550004

目的:筛查肝纤维化组织相关基因,探讨肝纤维化发生机制.方法:抽提肝纤维化组织和正常肝组织总RNA来制备探针,经杂交、洗涤后,通过计算机扫描分析肝纤维化组织和正常肝组织基因表达谱的差异情况,并用实时荧光定量PCR技术对其中部分差异... 详细信息

目的:筛查肝纤维化组织相关基因,探讨肝纤维化发生机制.方法:抽提肝纤维化组织和正常肝组织总RNA来制备探针,经杂交、洗涤后,通过计算机扫描分析肝纤维化组织和正常肝组织基因表达谱的差异情况,并用实时荧光定量PCR技术对其中部分差异基因的表达水平变化进行验证.结果:筛选出差异表达的基因68个,其中肝纤维化组织中表达上调的基因35个,表达下调的基因33个,这些基因按照功能可以分为调控细胞信号转导、DNA损伤与修复、转录调控因子、代谢相关基因以及未知功能基因.与正常肝组织比较,肝纤维化组织中C/EB Pβ mRNA表达下调(22.02±4.82vs59.13±8.21,P<0.01),MMP-14 mRNA表达上调(257.33±26.58vs21.65±4.37,P<0.01),结果与基因芯片一致.结论:多种基因参与肝纤维化的形成过程,肝纤维化组织与正常肝组织之间存在有明显的基因表达差异.

关键词：肝纤维化肝组织基因芯片基因表达实时荧光定量PCR技术

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乙型肝炎肝纤维化组织双向电泳技术的优化

世界华人消化杂志 2007年第13期15卷 1554-1557页

作者：罗新华杨勤张权程明亮贵阳医学院附属医院感染科贵州省贵阳市550004 贵阳医学院病理生理学教研室贵州省贵阳市550004

目的:建立并优化乙型肝炎肝纤维化组织蛋白质组研究所需的双向凝胶电泳技术,提高其分辨率和重复性.方法:外科手术获取乙型肝炎肝纤维化组织,提取肝组织总蛋白.利用固相pH梯度双向凝胶电泳技术分离总蛋白,考马斯亮蓝染色后分析凝胶图像.... 详细信息

目的:建立并优化乙型肝炎肝纤维化组织蛋白质组研究所需的双向凝胶电泳技术,提高其分辨率和重复性.方法:外科手术获取乙型肝炎肝纤维化组织,提取肝组织总蛋白.利用固相pH梯度双向凝胶电泳技术分离总蛋白,考马斯亮蓝染色后分析凝胶图像.对2-DE中的关键因素与环节,如样本处理、上样量、电泳参数等各步条件进行了一系列优化.结果:优化条件后的乙型肝炎肝纤维化组织双向凝胶电泳图谱水平拖尾减少、斑点分辨率改善、斑点检出数量增加(246±33→729±83).结论:该双向凝胶电泳技术可应用于乙型肝炎肝纤维化组织的蛋白质组学研究.

关键词：乙型肝炎肝纤维化组织双向凝胶电泳

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肝纤维化患者血清差异蛋白质的筛选

世界华人消化杂志 2009年第16期17卷 1672-1675页

作者：罗新华程明亮杨勤张权贵州省人民医院感染科贵州省贵阳市550002 贵阳医学院附属医院感染科贵州省贵阳市550004

目的:对比分析肝纤维化患者和健康人血清中差异表达的蛋白质,筛选与肝纤维化发生密切相关的血清蛋白质.方法:肝纤维化患者和健康人空腹血清标本各6例分别等量混合成2个样本,去除血清中的白蛋白和IgG.利用双向凝胶电泳(2-DE)分离肝纤维... 详细信息

目的:对比分析肝纤维化患者和健康人血清中差异表达的蛋白质,筛选与肝纤维化发生密切相关的血清蛋白质.方法:肝纤维化患者和健康人空腹血清标本各6例分别等量混合成2个样本,去除血清中的白蛋白和IgG.利用双向凝胶电泳(2-DE)分离肝纤维化血清和健康人血清总蛋白质后,硝酸银染色,经图像分析筛选差异表达的蛋白点.应用基质辅助激光解吸电离飞行时间质谱(MALDI-TOF-MS)鉴定差异表达的蛋白点,并用ELISA法对其中部分差异蛋白的表达水平进行验证.结果:筛选出2组间的差异蛋白点共517个,对其中表达量差异3倍以上的24个蛋白点进行MALDI-TOF-MS分析,鉴定出包括触珠蛋白、载脂蛋白A-Ⅳ、转铁蛋白、T细胞受体β链、血清白蛋白及血清白蛋白前体等8个蛋白质,其中3个蛋白质在肝纤维化血清中表达上调,5个蛋白质在肝纤维化血清中表达下调.与健康人血清比较,ELISA法检测肝纤维化血清中触珠蛋白、载脂蛋白A-Ⅳ表达均下调,结果与双向凝胶电泳一致.结论:肝纤维化血清与健康人血清的蛋白质表达谱表现出一定差异,这些差异表达的血清蛋白质有望成为肝纤维化诊断与判定预后的血清标志物.

关键词：肝纤维化血清蛋白质组学