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Trehalose attenuates abdominal aortic aneurysm formation by inducing autophagy in smooth muscle cells

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Journal of Geriatric Cardiology 2023年第3期20卷 214-222页

作者： Bo JIANG Xuan LI Mo wang guang-xin LI Peng-Wei REN Yu-Qi wang Shi-Jie xin Ling-Feng QIN Department of Vascular Surgery the First Hospital of China Medical UniversityKey Laboratory of PathogenesisPrevention and Therapeutics of Aortic AneurysmShenyangChina Department of Surgery Yale School of MedicineNew HavenCTUSA Department of Breast and Thyroid Surgery Peking University Shenzhen HospitalShenzhenChina Yale Stem Cell Center Yale School of MedicineNew HavenCTUSA Department of Biomedical Engineering Yale UniversityNew HavenCTUSA

BACKGROUND Trehalose is a naturally occurring disaccharide,which has been identified as an autophagy inducer and ex-hibits protective effect in cardiovascular diseases such as myocardial infraction and atherosclerosis.However,the functional role of trehalose in abdominal aortic aneurysm(AAA)remains undefined.METHODS To study the effect of trehalose in AAA,trehalose(1 g/kg per day)were given for 14 continuous days in a mouse model of elastase-induced abdominal aortic aneurysm.On day 14,ultrasound was performed to measure aortic diameter before the abdominal aortas were harvested and processed for further analysis.Verhoeff-Van Gieson staining and TUNEL staining were performed on paraffin sections to evaluate vascular histology and apoptosis,immunofluorescence staining and Western-blot were performed to evaluate expression of autophagy markers.RESULTS Echocardiography and in situ pictures demonstrated that trehalose attenuated infrarenal aorta dilation.Verhoeff-Van Gieson staining showed elastin degradation was improved in trehalose-treated group.Compared with vehicle-treated mice,trehalose treatment restored smooth muscle cell contractile phenotype with increasedα-SMA,Calponin and Myh11 expression.Furthermore,trehalose also attenuated cell apoptosis and leukocytes infiltration.Importantly,trehalose induced autophagy with decrease SQSTM1/p62 accumulation,increased lamp2 expression and LC3B conversion.CONCLUSION Trehalose attenuated AAA progression with decreased inflammation and restored SMC contractile phenotype by inducing autophagy.These results demonstrated the therapeutic potential of trehalose in AAA.

关键词： abdominal aneurysm protective

The ornithine-urea cycle involves fumaric acid biosynthesis in Aureobasidium pullulans var.aubasidani,a green and eco-friendly process for fumaric acid production

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Synthetic and Systems Biotechnology 2023年第1期8卷 33-45页

作者： xin Wei Miao Zhang guang-Yuan wang guang-Lei Liu Zhen-Ming Chi Zhe Chi College of Marine Life Sciences Ocean University of ChinaYushan RoadNo.5QingdaoChina Laboratory for Marine Biology and Biotechnology Qingdao National Laboratory for Marine Science and Technology266003China College of Life Science Shandong Province Key Laboratory of Applied MycologyQingdao Agricultural UniversityQingdao266109China

The current petroleum chemical methods for fumaric acid production can cause heavy pollution and global warming.In this study,the engineered strains of A.pullulans var.aubasidani were found to be suitable for green fumaric acid producer.Removal and complementation of the relevant genes showed only the ornithine-urea cycle(OUC)was involved in high level fumarate biosynthesis which was controlled by the Ca^(2+)signaling pathway.Removal of both the GOX gene encoding glucose oxidase and the PKS1 gene encoding the polyketide synthase for 3,5-dihydroxydecanoic acid biosynthesis and overexpression of the PYC gene encoding pyruvate carboxylase made the strain e-PYC produce 88.1±4.3 g/L of fumarate at flask level and 93.9±0.8 g/L of fumarate during the fed-batch fermentation.As a yeast-like fungal strain,it was very easy to cultivate A.pullulans var.aubasidani DH177 and their mutants in the bioreactor and to edit its genomic DNAs to enhance fumarate production.It was found that 2 mol of CO_(2) could be fixed during a maximal theoretical yield of 2 mol of fumarate per mole of glucose consumed in the OUC.Therefore,the OUC-mediated fumarate biosynthesis pathway in A.pullulans var.aubasidani was a green and eco-friendly process for the global sustainable development and carbon neutrality.

关键词： Fumaric acid A.pullulans var.aubasidani Ornithine-urea cycle Ca^(2+)signaling pathway

Effect of End Shape on Flow and Heat Transfer Characteristics of Two Parallel Plates

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Journal of Thermal Science 2024年第3期33卷 815-832页

作者： ZHANG guang HONG Tianxiao wang Dongrui xin Jialin TAO Junyu LIN Zhe Key Laboratory of Fluid Transmission Technology of Zhejiang Province Zhejiang Sci-Tech UniversityHangzhou310018China

Flow around a pair of flat plates is a basic hydrodynamics problem.In this paper,the flow and heat transfer characteristics of two parallel plates with different edge shapes are numerically calculated.Under different inclined angles,the influence of chamfered and rounded structures with different sizes at the end-edge on unsteady flow and heat transfer characteristics of two parallel plates are analyzed.It is found that the instability and unsteadiness of flow decrease with the increase of end-edge size,and the non-uniformity of wake velocity of both rounded and chamfered plates decreases gradually.The non-uniformity of wake temperature increases firstly and then decreases at a small inclined angle,and the amplitude becomes the largest when S_(rou)(S_(cha))=3,while it basically keeps monotonically increasing at a large inclined angle.Moreover,the global heat transfer performance of the flat plate is obviously affected by the end-edge modification,especially the chamfered structure.With the increase of chamfered size,the global Nusselt number basically shows the decreasing trend.This study provides a theoretical basis for the application of plate-shape structure in engineering fields.

关键词： two parallel plates end shape flow characteristic heat transfer numerical simulation

A Data Mining-Based Study on Medication Rules of Chinese Herbs to Treat Heart Failure with Preserved Ejection Fraction

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Chinese Journal of Integrative Medicine 2022年第9期28卷 847-854页

作者： GUO Hong-xin wang Jian-ru PENG guang-cao LI Ping ZHU Ming-jun Henan University of Chinese Medicine Zhengzhou450046China The First Affiliated Hospital of Henan University of Chinese Medicine Zhengzhou450099China

Objective:To summarize the medication rules of Chinese herbs to treat heart failure with preserved ejection fraction(HFPEF)based on data mining and to provide references for clinical utilization.Methods:The China National Knowledge Infrastructure(CNKI),Wanfang database(Wanfang),VIP database(VIP),Chinese Biomedical Literature(CBM),PubMed,Embase,and Cochrane Library databases were searched from inception to October 2021 to identify relevant literature on treating HFPEF with Chinese herbs.Microsoft Excel 2019 was used to set up a database,and then,association rule analysis and hierarchical cluster analysis were performed by using apriori algorithm and hclust function respectively in R-Studio(Version 4.0.3).Results:A total of 182 qualified papers were included,involving a total of 92 prescriptions,130 Chinese herbs,and 872 individual herbs prescribed,with an average of 9.5 herbs per prescription.The six most frequently prescribed herbs were Astragali Radix(Huangqi),Salviae Miltiorrhizae Radix Et Rhizoma(Danshen),Poria(Fuling),Glycyrrhizae Radix Et Rhizoma(Gancao),Cinnamomi Ramulus(Guizhi),and Ginseng Radix Et Rhizoma(Renshen).There were 35 herbs used more than 5 times,involving 11 efficacy categories.The top three categories were deficiency-tonifying herbs,blood-activating and stasis-removing herbs,and dampnessdraining diuretic herbs.The most commonly used herbs were mainly warm and sweet.The primary meridian tropisms were Lung Meridian,Heart Meridian and Spleen Meridian.Association rule analysis yielded 26 association rules,such as Astragali Radix(Huangqi)&Salviae Miltiorrhizae Radix Et Rhizoma(Danshen),Poria(Fuling),Cinnamomi Ramulus(Guizhi)&Atractylodis Macrocephalae Rhizoma(Baizhu).Hierarchical cluster analysis yielded four herb classes,and their functions were mainly qi-replenishing and yang-warming,bloodactivating and diuresis-inducing.Conclusions:HFPEF is the syndrome of root vacuity and tip repletion,and its core pathogenesis is"deficiency","stasis",and"wafer",with"deficiency"being the most principal,which is

关键词： heart failure with preserved ejection fraction data mining Chinese herbs medication rules association rules cluster analysis

Oscillating field stimulation promotes axon regeneration and locomotor recovery after spinal cord injury

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Neural Regeneration Research 2022年第6期17卷 1318-1323页

作者： Yi-xin wang Jin-Zhu Bai Zhen Lyu guang-Hao Zhang Xiao-Lin Huo Department of Spine and Spinal Cord Surgery Beijing Bo’ai HospitalRehabilitation Research CenterBeijingChina School of Rehabilitation Medicine Capital Medical UniversityBeijingChina Beijing Key Laboratory of Bioelectromagnetism Institute of Electrical EngineeringChinese Academy of SciencesBeijingChina School of Electronics Electrical and Communication EngineeringUniversity of Chinese Academy of SciencesBeijingChina

Oscillating field stimulation(OFS)is a potential method for treating spinal cord injury.Although it has been used in spinal cord injury(SCI)therapy in basic and clinical studies,its underlying mechanism and the correlation between its duration and nerve injury repair remain poorly understood.In this study,we established rat models of spinal cord contusion at T10 and then administered 12 weeks of OFS.The results revealed that effectively promotes the recovery of motor function required continuous OFS for more than 6 weeks.The underlying mechanism may be related to the effects of OFS on promoting axon regeneration,inhibiting astrocyte proliferation,and improving the linear arrangement of astrocytes.This study was approved by the Animal Experiments and Experimental Animal Welfare Committee of Capital Medical University(supplemental approval No.AEEI-2021-204)on July 26,2021.

关键词： astrocyte orientation astrocyte proliferation axonal regeneration locomotor recovery neural regeneration neural repair oscillating field stimulation spinal cord injury stimulus duration

Antarctic ice-sheet near-surface snowmelt detection based on the synergy of SSM/I data and QuikSCAT data

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Geoscience Frontiers 2018年第3期9卷 955-963页

作者： xing-Dong wang xin-Wu Li Cheng wang xin-guang Li College of Information Science and Engineering Henan University of Technology Key Laboratory of Digital Earth Institute of Remote Sensing & Digital EarthChinese Academy of Sciences

Microwave radiometer SSM/I data and scatterometer QuikSCAT data have been widely used for the icesheet near-surface snowmelt detection based on their sensitivity to liquid water present in snow. In order to improve the Antarctic ice-sheet near-surface snowmelt detection accuracy, a new Antarctic icesheet near-surface snowmelt synergistic detection method was proposed based on the principle of complementary advantages of SSM/I data(high reliability) and QuikSCAT data(high sensitivity) by the use of edge detection model to automatically extract the edge information to get the distribution of Antarctic snowmelt onset date, snowmelt duration and snowmelt end date. The verification result shows that the proposed snowmelt synergistic detection method improves the detection accuracy from about 75% to 86% based on AWS(Automatic Weather Stations) Butler Island and Larsen Ice Shelf. The algorithm can also be applied to other regions, which provides methodological support and supplement for the global snowmelt detection.

关键词： Snowmelt detection SSM/I data QuikSCAT data Synergy Edge detection model

Constructing Tertiary Alcohols with Vicinal Stereocenters:Highly Diastereo-and Enantioselective Cyanosilylation of α-Branched Acyclic Ketones and Their Kinetic Resolution

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CCS Chemistry 2022年第6期4卷 2140-2152页

作者： Wen-Biao Wu xin Yu Jin-Sheng Yu xin wang Wen-guang wang Jian Zhou Shanghai Engineering Research Center of Molecular Therapeutics and New Drug Development Shanghai Key Laboratory of Green Chemistry and Chemical ProcessesEast China Normal UniversityShanghai 200062 School of Chemistry and Chemical Engineering Shandong UniversityJinan 250100 College of Chemistry Sichuan UniversityChengdu 610064 State Key Laboratory of Organometallic Chemistry Shanghai Institute of Organic ChemistryShanghai 200032

We report the first highly diastereo-and enantioselective C-C bond-forming reaction of racemic α-branched ketones to construct tertiary alcohols with adjacent stereocenters.Accordingly,a highly stereoselective cyanosilylation of racemic ketones is developed using our bifunctional cyanating reagent,Me_(2)(CH_(2)Cl)SiCN,giving C^(α)-tetrasubstituted silyl cyanohydrins withtwovicinal stereocenters inupto>20:1 diastereomeric ratio(dr)and 90-98% enantiomeric excess(ee)values,which can undergo various diversification reactions bymanipulating the chloromethyl group.A highly selective kinetic resolution of acyclicα-branched ketones is also developed that allows facile access to acyclic α-alkyl,allyl,and propargyl ketones with good recovery and excellent ee values.The synthetic value of this protocol is further demonstrated by the formal synthesis of the anti-obesity agent,taranabant(MK-0364).The activation of Jacobsen’s privileged catalyst(salen)AlCl by a suitable phosphorane plays a crucial role in the reaction.X-ray crystallographic analysis of single crystals of phosphorane-(salen)AlCl complexes and theoretical calculations help provide a working model.The present transformation opens a new path for the catalytic stereoselective synthesis of stereochemically complex tertiary alcohols featuring two stereocenters(adjacent or not)from racemic ketones.

关键词： tertiary alcohols with vicinal stereocenters ketone cyanosilylation kinetic resolution

Amphioxus Gli knockout disrupts the development of left–right asymmetry but has limited impact on neural patterning

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Marine Life Science & Technology 2023年第4期5卷 492-499页

作者： xin Huang Qiongqiong Ren Yiquan wang Sebastian M.Shimeld guang Li State Key Laboratory of Cellular Stress Biology School of Life SciencesXiamen UniversityXiamen 361005China Department of Zoology University of OxfordOxford OX13SZUK

The Gli transcription factors are the primary mediators of Hedgehog(Hh)signaling.Vertebrate genomes contain multiple Gli paralogues with different functions downstream of Hh signal receipt,in part explaining the complexity of cellular responses to Hh that allow concentration-dependent target gene activation.Amphioxus is a chordate that split from the vertebrate lineage early in the evolution of chordates,before the genome duplications that occurred in early vertebrate evolution.It has a single Gli gene whose transcripts can be alternately spliced to yield two protein isoforms called GliS and GliL.We generated two knockout mutations in amphioxus Gli,one that affects the whole gene and a second that only affects GliL.Both knockouts showed major morphological and molecular defects in the development of left–right asymmetry,a phenotype that is similar but not identical to that previously found in Hh mutants.Hh signaling also patterns the amphioxus neural tube.Here,however,knockout of GliL showed no identifiable phenotype,while knockout of the full gene showed only small changes to the expression of one gene family,Olig.Other genes that were prominently affected by Hh knockout were not altered in expression in either knockout.Reasons for the differences between Hh and Gli knockouts in the pharynx and neural tube are discussed in the context of the likely different functions of amphioxus Gli isoforms.

关键词： Hh signaling pathway Chordates Embryonic development Evolution

Ultrathin self-assembly MXene/Co-based bimetallic oxide heterostructures as superior and modulated microwave absorber

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Journal of Materials Science & Technology 2023年第3期134卷 132-141页

作者： Hui-Ya wang Xiao-Bo Sun Yue xin Shu-Hao Yang Peng-Fei Hu guang-Sheng wang School of Chemistry Beihang UniversityBeijing 102206China Research Institute of Aero-Engine Beihang UniversityBeijing 102206China

Lightweight and high-efficiency absorbing materials equipped with tunable electromagnetic properties are the foremost important factors for driving applications of 5 G smart era and military stealth.Stim-ulated by the remarkable merits of heterogeneous interface engineering,a series of unique flower-like Co-based multiphases nanostructures were successfully synthesized through identical hydrothermal method,and subsequently anchored on the Ti_(3)C_(2)T_(x)nanosheets.The suitable metal ion modulation is feasible to regulate the components,heterogeneous interfaces and defects for tailoring the electromag-netic parameters and microwave absorption(MA)property.Notably,the permittivity decreases of CMOT(CoO/MCo_(2)O_(4)/Ti_(3)C_(2)T_(x),M=Fe,Cu,Zn)composites proceed gradually in the order of Fe^(2+),Cu^(2+),Zn^(2+),while the MA performance with tunable absorption peak could be modulated subtly.The asobtained CFOT-12(10 wt%),CCOT-15(10 wt%)and CZOT-15(15 wt%)exhibit excellent MA capacity with a minimum reflection loss(RL_(min))of-41.06,-52.67,-52.52 dB and corresponding effective absorption bandwidth(EAB)of 3.68,4.48,3.92 GHz at 2.10,1.90,1.80 mm,respectively.This work expands a novel approach to construct multicomponent heterostructure for tuning the EM parameters and absorbing property,as well as realize the high efficient and light weight.

关键词： Heterogeneous interface Modulation MXene High-efficiency absorbing materials Lightweight