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A Bioorthogonal Post-modification of N^(6)-Isopentenyl Adenosine i^(6) A RNA in Live Cells

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CCS Chemistry 2024年第9期6卷 2241-2253页

作者： Li-Jun Xie Hou-ping cheng Meng-Yuan chen xin wang Li Liu Bi-Feng Yuan Liang cheng Beijing National Laboratory for Molecular Sciences(BNLMS) CAS Key Laboratory of Molecular Recognition and FunctionCAS Research/Education Center for Excellence in Molecular Sciences Institute of ChemistryChinese Academy of SciencesBeijing 100190 School of Public Health Wuhan UniversityWuhan 430071 University of Chinese Academy of Sciences Beijing 100049

Selective methods for modulating RNA epigenetic modifications within living cells and organisms represent attractive techniques for investigating biological functions and medicinal *** contrast to enzymatic methods,abiotic chemical modulation offers access to diverse new-to-natural *** herein report a visible light-assisted bioorthogonal reaction involving flavin mononucleotide,sodium azide,and blue light *** concert with previous chemical approaches mimicking the demodification pathway,our system functions as a powerful and selective post-modification enzyme that targets N^(6)-prenyl adenosine i^(6) A in RNA and enables the efficient construction of an artificial N^(6)-cyanomethyl adenosine(cnm^(6) A).Notably,most endogenous biomacromolecular functions,including other common RNA epigenetics,remained ***,we have successfully modified i^(6) A in tRNA to cnm^(6) A in human cells and establish this system as a bona fide bioorthogonal reaction with potential applications in chemical biology and in-cell modulation.

关键词： chemical post-modification bioorthogonal reactions RNA modifications N^(6)-isopentenyl adenosine N^(6)-cyanomethyl adenosine

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关于安全案例论证构建的综述

软件学报 2024年第9期35卷 4013-4037页

作者：陈泽众邓玉欣上海市高可信计算重点实验室(华东师范大学) 上海200062

安全案例提供清晰、全面和可靠的论据,说明系统在特定环境下的操作满足可接受的安全性.在受监管的安全攸关领域,如汽车、航空和核能等领域,认证机构通常要求系统经过严格的安全评估程序,以确保其符合一个或多个安全标准.在系统开发中应... 详细信息

安全案例提供清晰、全面和可靠的论据,说明系统在特定环境下的操作满足可接受的安全性.在受监管的安全攸关领域,如汽车、航空和核能等领域,认证机构通常要求系统经过严格的安全评估程序,以确保其符合一个或多个安全标准.在系统开发中应用安全案例是一种新兴的技术手段,以结构化和全面的方式表达安全攸关系统的安全属性.对安全案例的4个基本构建步骤:确定目标、收集证据、构建论证和评估安全案例,进行简要介绍.然后聚焦于构建论证这一关键步骤,详细介绍现有的8种安全案例表达形式,包括目标结构符号(GSN)、声明-论点-证据(CAE)、结构化安全案例元模型(SACM)等,并分析了它们的优缺点.由于安全案例所需材料的显著复杂性,软件工具通常被用作构建和评估安全案例的实用方法.比较7种用于安全案例开发和评估的工具,包括astah system safety、gsn2x、NOR-STA、Socrates、ASCE、D-Case Editor和AdvoCATE.此外,还深入探讨了安全案例构建中所面临的多重挑战,这些挑战包括数据的可靠性和完整性、复杂性和不确定性的管理、监管和标准的不一致、人因工程、技术的快速发展以及团队和跨学科合作6个方面.最后,展望安全案例的未来研究方向,揭示其潜在应用和研究问题.

关键词：安全案例系统安全论证构建目标结构符号安全案例工具

Bifunctional TiO_(2-x)nanofibers enhanced gel polymer electrolyte for high performance lithium metal batteries

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Journal of Energy Chemistry 2024年第2期89卷 437-448,I0011页

作者： Yixin Wu Zhen chen yang wang Yu Li Chunxing Zhang Yihui Zhu Ziyu Yue xin Liu Minghua chen Key Laboratory of Engineering Dielectric and Applications(Ministry of Education) School of Electrical and Electronic EngineeringHarbin University of Science and TechnologyHarbin 150080HeilongjiangChina

Exploration of advanced gel polymer electrolytes(GPEs)represents a viable strategy for mitigating dendritic lithium(Li)growth,which is crucial in ensuring the safe operation of high energy density Li metal batteries(LMBs).Despite this,the application of GPEs is still hindered by inadequate ionic conductivity,low Li^(+)transference number,and subpar physicochemical ***,Ti O_(2-x)nanofibers(NF)with oxygen vacancy defects were synthesized by a one-step process as inorganic fillers to enhance the thermal/mechanical/ionic-transportation performances of composite *** characterizations and theoretical calculations reveal that the oxygen vacancies on the surface of Ti O_(2-x)NF accelerate the dissociation of Li PF_6,promote the rapid transfer of free Li^(+),and influence the formation of Li F-enriched solid electrolyte ***,the composite GPEs demonstrate enhanced ionic conductivity(1.90m S cm^(-1)at room temperature),higher lithium-ion transference number(0.70),wider electrochemical stability window(5.50 V),superior mechanical strength,excellent thermal stability(210℃),and improved compatibility with lithium,resulting in superior cycling stability and rate performance in both Li||Li,Li||Li Fe PO_(4),and Li||Li Ni_(0.8)Co_(0.1)Mn_(0.1)O_(2)***,the synergistic influence of nanofiber morphology and enriched oxygen vacancy structure of fillers on electrochemical properties of composite GPEs is comprehensively investigated,thus,it is anticipated to shed new light on designing high-performance GPEs LMBs.

关键词： Nanofibers fillers Oxygen vacancies Gel polymer electrolytes Lithium metal batteries

Preparation of yttrium oxide coating with enhanced thermal shock resistance on refractories used for titanium alloy melting and casting

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Journal of Iron and Steel Research International 2024年第6期31卷 1411-1422页

作者： Ao chen yan Ma Zhou-fu wang Hao Liu Xi-tang wang Sen-na chen chen-xin Zhang Zi-yi Tang Wei-dong Fu Wei cheng The State Key Laboratory of Refractories and Metallurgy Wuhan University of Science and TechnologyWuhan 430081HubeiChina Xiangyang Juli High Technology Material Co. Ltd.Xiangyang 441705HubeiChina Xiangyang Public Inspection and Testing Center Xiangyang 441000HubeiChina

In order to enhance the service life of refractories for melting and casting of titanium alloy,preparation of yttrium oxide coatings on the refractories surface is an effective *** improvement of thermal shock stability of the coating is beneficial to its spalling resistance during the high-temperature service,and the available slurry with good performance is the *** effects of the varieties and contents of dispersant(sodium tripolyphosphate,sodium hexametaphosphate,sodium pyrophosphate,citric acid and polycarboxylate ammonium salt)and binder(aluminum dihydrogen phosphate,zirconium acetate and yttria sol)on the stability,rheological and thixotropic properties of yttrium oxide waterbased slurry were investigated,and the effects of slurry composition design on the thermal shock resistance of the coating were *** results showed that the introduction of polycarboxylate ammonium salt as dispersant significantly improved the stability of the slurry,and the varieties of the binders had significant effects on the dispersibility of the *** aluminum dihydrogen phosphate and polycarboxylate ammonium salt were used as binder and dispersant,respectively,the slurry had better stability,suitable rheological and thixotropic *** using aluminum dihydrogen phosphate as binder,the coating had good thermal shock resistance and no obvious cracks were observed for the coating after thermal shock,which was attributed to the yttrium aluminate binding phase generated in the *** effective method was provided for preparing yttrium oxide coatings with enhanced thermal shock resistance applied in the process of titanium alloy melting and casting.

关键词： Yttrium oxide-Slurry Stability Rheological property Thixotropic property-Coating Thermal shock resistance

Pressure stimulated current in progressive failure process of combined coal-rock under uniaxial compression:Response and mechanism

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International Journal of Mining Science and Technology 2024年第2期34卷 227-243页

作者： Tiancheng Shan zhonghui Li xin Zhang Haishan Jia Xiaoran wang Enyuan wang Yue Niu Dong chen Weichen Sun Dongming wang State Key Laboratory of Coal Mine Disaster Prevention and Control China University of Mining and TechnologyXuzhou 221116China National Engineering Research Center for Coal Gas Control China University of Mining and TechnologyXuzhou 221116China School of Safety Engineering China University of Mining and TechnologyXuzhou 221116China State Key Laboratory for Fine Exploration and Intelligent Development of Coal Resources China University of Mining and TechnologyXuzhou 221116China State Key Laboratory of Intelligent Construction and Healthy Operation and Maintenance of Deep Underground Engineering China University of Mining and TechnologyXuzhou 221116China

Effective monitoring of the structural health of combined coal-rock under complex geological conditions by pressure stimulated currents(PSCs)has great potential for the understanding of dynamic disasters in underground *** reveal the effect of this way,the uniaxial compression experiments with PSC monitoring were conducted on three types of coal-rock combination samples with different strength *** mechanism explanation of PSCs are investigated by resistivity test,atomic force microscopy(AFM)and computed tomography(CT)methods,and a PSC flow model based on progressive failure process is *** influence of strength combinations on PSCs in the progressive failure process are *** results show the PSC responses between rock part,coal part and the two components are different,which are affected by multi-scale fracture characteristics and electrical *** the rock strength decreases,the progressive failure process changes obviously with the influence range of interface constraint effect decreasing,resulting in the different responses of PSC strength and direction in different parts to fracture *** PSC flow model is initially validated by the relationship between the accumulated charges of different *** results are expected to provide a new reference and method for mining design and roadway quality assessment.

关键词： Combined coal-rock Pressure stimulated current Progressive failure process Mechanism Flow model

Adaptive patient-cooperative compliant control of lower limb rehabilitation robot

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Biomimetic Intelligence & Robotics 2024年第2期4卷 42-49页

作者： Lingling chen Jjiabao Huang yanglong wang Shijie Guo Mengge wang xin Guo School of Artificial Intelligence Hebei University of TechnologyTianjin 300400China Inteligent Rehabilitation Device and Detection Technology Engineering Research Centre of the Ministry of Education Tianjin 300400China School of Mechanical Engineering Hebei University of TechnologyTianjin 300400China School of Electrical Engineering Hebei University of TechnologyTianjin 300400China

With the increase in the number of stroke patients,there is a growing demand for rehabilitation ***-assisted training is expected to play a crucial role in meeting this *** ensure the safety and comfort of patients during rehabilitation training,it is important to have a patient-cooperative compliant control system for rehabilitation *** order to enhance the motion compliance of patients during rehabilitation training,a hierarchical adaptive patient-cooperative compliant control strategy that includes patient-passive exercise and patient-cooperative exercise is proposed.A low-level adaptive backstepping position controller is selected to ensure accurate tracking of the desired *** the high-level,an adaptive admittance controller is employed to plan the desired trajectory based on the interaction force between the patient and the *** results of the patient-robot cooperation experiment on a rehabilitation robot show a significant improvement in tracking trajectory,with a decrease of 76.45%in the dimensionless squared jerk(DSJ)and a decrease of 15.38%in the normalized root mean square deviation(NRMSD)when using the adaptive admittance *** proposed adaptive patient-cooperative control strategy effectively enhances the compliance of robot movements,thereby ensuring the safety and comfort of patients during rehabilitation training.

关键词： Compliant control Lower limb rehabilitation robot(LLRR) Adaptive admittance controller Adaptive backstepping controller Human-robot interaction

Dynamic membrane filtration accelerates electroactive biofilms in bioelectrochemical systems

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Environmental Science and Ecotechnology 2024年第4期20卷 208-217页

作者： Jinning wang Mei chen Jiayao Zhang xinyi Sun Nan Li xin wang MOE Key Laboratory of Pollution Processes and Environmental Criteria Tianjin Key Laboratory of Environmental Remediation and Pollution ControlCollege of Environmental Science and EngineeringNankai UniversityNo.38 Tongyan RoadJinnan DistrictTianjin300350China School of Environmental Science and Engineering Tianjin UniversityNo.35 Yaguan RoadJinnan DistrictTianjin300350China

Bioelectrochemical systems(BES)have emerged as a dual-function technology for treating wastewater and recovering energy.A vital element of BES is the rapid formation and maintenance of electroactive biofilms(EABs).Previous attempts to accelerate EAB formation and improve electroactivities focused on enhancing the bacterial adhesion process while neglecting the rate-limiting step of the bacterial transport ***,we introduce membrane filtration into BES,establishing a dynamic membrane filtration system that enhances overall *** observed that optimal membrane flux considerably reduced the startup time for EAB ***,EABs established under a 25 L m^(-2)h^(-1)flux(EAB_(25)LMH)had a formation time of 43.8±1.3 h,notably faster than the 51.4±1.6 h in the static state(EAB_(0)LMH).Additionally,EAB_(25)LMH exhibited a significant increase in maximum current density,approximately 2.2 times higher than EAB_(0)*** correlation analysis indicated a positive relationship between current densities and biomass quantities and an inverse correlation with startup *** analysis revealed two critical findings:(i)variations in maximum current densities across different filtration conditions were associated with redox-active substances and biomass accumulation,and(ii)the incorporation of a filtration process in EAB formation enhanced the proportion of viable cells and encouraged a more diverse range of electroactive ***,the novel electroactive membrane demonstrated sustained current production and effective solid-liquid separation during prolonged operation,indicating its potential as a viable alternative in membrane-based *** approach not only provides a new operational model for BES but also holds promise for expanding its application in future wastewater treatment solutions.

关键词： Electroactive biofilm Membrane filtration Spatial structure Mass transfer Microbial community

Alkaline sphingomyelinase deficiency impairs intestinal mucosal barrier integrity and reduces antioxidant capacity in dextran sulfate sodium-induced colitis

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World Journal of Gastroenterology 2024年第10期30卷 1405-1419页

作者： Ye Tian xin Li Xu wang Si-Ting Pei Hong-xin Pan Yu-Qi cheng Yi-chen Li Wen-Ting Cao Jin-Dong Ding Petersen ping Zhang International School of Public Health and One Health Hainan Medical UniversityHaikou 571199Hainan ProvinceChina Medical Laboratory Science and Technology College Harbin Medical University-Daqing CampusDaqing 163000Heilongjiang ProvinceChina Department of Laboratory Diagnosis Qiqihar Tuberculosis Control CenterQiqihar 161000Heilongjiang ProvinceChina Department of Public Health University of CopenhagenCopenhagen 1353Denmark Department of Public Health University of Southern DenmarkOdense 5000Denmark

BACKGROUND Ulcerative colitis is a chronic inflammatory disease of the colon with an unknown *** sphingomyelinase(alk-SMase)is specifically expressed by intestinal epithelial cells,and has been reported to play an anti-inflammatory ***,the underlying mechanism is still *** To explore the mechanism of alk-SMase anti-inflammatory effects on intestinal barrier function and oxidative stress in dextran sulfate sodium(DSS)-induced *** Mice were administered 3%DSS drinking water,and disease activity index was determined to evaluate the status of *** permeability was evaluated by gavage administration of fluorescein isothiocyanate dextran,and bacterial translocation was evaluated by measuring serum *** epithelial cell ultrastructure was observed by electron *** blotting and quantitative real-time reverse transcription-polymerase chain reaction were used to detect the expression of intestinal barrier proteins and mRNA,*** oxidant and antioxidant marker levels were analyzed using commercial kits to assess oxidative stress *** Compared to wild-type(WT)mice,inflammation and intestinal permeability in alk-SMase knockout(KO)mice were more severe beginning 4 d after DSS *** mRNA and protein levels of intestinal barrier proteins,including zonula occludens-1,occludin,claudin-3,claudin-5,claudin-8,mucin 2,and secretory immunoglobulin A,were significantly reduced on 4 d after DSS *** observations revealed progressive damage to the tight junctions of intestinal epithelial ***,by day 4,mitochondria appeared swollen and ***,compared to WT mice,serum malondialdehyde levels in KO mice were higher,and the antioxidant capacity was significantly *** expression of the transcription factor nuclear factor erythroid 2-related factor 2(Nrf2)in the colonic mucosal tissue of KO mice was significantly decr

关键词： Alkaline sphingomyelinase Intestinal mucosal barrier Antioxidant capacity Dextran sulfate sodium-induced colitis nuclear factor erythroid 2-related factor 2

High-efficiency sodium storage of Co_(0.85)Se/WSe_(2) encapsulated in N-doped carbon polyhedron via vacancy and heterojunction engineering

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Carbon Energy 2024年第1期6卷 94-107页

作者： ya Ru Pei Hong Yu Zhou Ming Zhao Jian chen Li xin Ge Wei Zhang Chun cheng yang Qing Jiang Key Laboratory of Automobile Materials(Jilin University) Ministry of EducationSchool of Materials Science and EngineeringJilin UniversityChangchunChina Jilin Provincial International Cooperation Key Laboratory of High-Efficiency Clean Energy Materials Electron Microscopy CenterJilin UniversityChangchunChina

With the advantage of fast charge transfer,heterojunction engineering is identified as a viable method to reinforce the anodes'sodium storage ***,vacancies can effectively strengthen the Na+adsorption ability and provide extra active sites for Na+***,their synchronous engineering is rarely ***,a hybrid of Co_(0.85)Se/WSe_(2) heterostructure with Se vacancies and N-doped carbon polyhedron(CoWSe/NCP)has been fabricated for the first time via a hydrothermal and subsequent selenization *** aberration-corrected transmission electron microscopy confirms the phase interface of the Co_(0.85)Se/WSe_(2) heterostructure and the existence of Se *** functional theory simulations reveal the accelerated charge transfer and enhanced Na+adsorption ability,which are contributed by the Co_(0.85)Se/WSe_(2) heterostructure and Se vacancies,*** expected,the CoWSe/NCP anode in sodium-ion battery achieves outstanding rate capability(339.6 mAh g^(−1) at 20 A g^(−1)),outperforming almost all Co/W-based selenides.

关键词： Co_(0.85)Se/WSe_(2)heterostructure density functional theory simulations N-doped carbon polyhedron Se vacancies sodium-ion batteries