检索结果-南通市图书馆

在线全文

学校读者我要写书评

暂无评论

两岸蔬菜行业标准比对及共通对策研究

标准科学 2024年第4期 122-128页

作者：林新萍厦门市标准化研究院

蔬菜是居民生活必不可少的农产品,两岸蔬菜及其制品贸易对菜农至关重要,但是内地出口至台湾地区的蔬菜中时常因两岸安全标准差异而受阻。在两岸融合发展的大背景下,本文以蔬菜为研究对象,对两岸蔬菜相关标准进行比对分析,为两岸蔬菜质... 详细信息

蔬菜是居民生活必不可少的农产品,两岸蔬菜及其制品贸易对菜农至关重要,但是内地出口至台湾地区的蔬菜中时常因两岸安全标准差异而受阻。在两岸融合发展的大背景下,本文以蔬菜为研究对象,对两岸蔬菜相关标准进行比对分析,为两岸蔬菜质量安全管控提供参考,并提出两岸蔬菜标准共通的建议。

关键词：蔬菜标准差异标准共通

维普期刊数据库博看期刊

细胞分裂周期蛋白73基因缺失抑制粟酒裂殖酵母细胞的有性生殖和有丝分裂

在线全文

学校读者我要写书评

暂无评论

中国生物化学与分子生物学报 2024年第6期40卷 807-818页

作者：刘梦楠白鑫余雯李欣霖丁祥侯怡铃西华师范大学生命科学学院西南野生动植物资源保护教育部重点实验室四川南充637009 西华师范大学环境科学与工程学院嘉陵江流域生态环境保护与污染防治南充市重点实验室四川南充637009

cdc73基因编码粟酒裂殖酵母(Schizosaccharomyces Pombe)RNA聚合酶Ⅱ辅助因子Cdc73,参与G_(2)期检查点激活并调控细胞周期。但cdc73基因缺失对细胞有丝分裂动力学的调控尚不清楚。本研究采用活细胞成像、荧光蛋白标记技术探究粟酒裂殖酵... 详细信息

cdc73基因编码粟酒裂殖酵母(Schizosaccharomyces Pombe)RNA聚合酶Ⅱ辅助因子Cdc73,参与G_(2)期检查点激活并调控细胞周期。但cdc73基因缺失对细胞有丝分裂动力学的调控尚不清楚。本研究采用活细胞成像、荧光蛋白标记技术探究粟酒裂殖酵母cdc73基因缺失后对细胞有性生殖和细胞有丝分裂中微管、肌动蛋白、线粒体和组蛋白动力学的影响。结果表明:在有性生殖中,cdc73基因缺失会导致子囊孢子长度增加14.23%,产4个孢子数量的细胞减少64.08%;活细胞成像结果分析发现,在有丝分裂中,分裂后期微管伸长的长度缩短11.21%,伸长时间减少17.39%;肌动蛋白环形成和收缩速率分别降低33.33%和26.09%,形成和收缩时间分别延长58.00%和40.38%;同时,肌动蛋白环、线粒体和组蛋白表达量都增加。本研究揭示了cdc73基因缺失可抑制有丝分裂中纺锤体的伸长,延缓肌动蛋白环的形成与收缩,为进一步探寻Cdc73蛋白在细胞分裂中参与调控微管和肌动蛋白动力学功能提供了一定的科学依据。

关键词： cdc 73基因粟酒裂殖酵母有性生殖有丝分裂细胞动力学

Function and regulation of muscle stem cells in skeletal muscle development and regeneration:a narrative review

在线全文

学校读者我要写书评

暂无评论

Journal of Bio-X Research 2021年第3期4卷 89-96页

作者： Han Zhu xin lin Yarui Diao Departments of Pediatrics and Cellular&Molecular Medicine Pediatric Diabetes Research CenterSanford Consortium for Regenerative MedicineUniversity of CalformiaSan DiegoLa JollaCA Department of Cell Biology Duke University Medical CenterDurhamNCUSA Regeneration Next Iitiative Duke University Medical CenterDurhamNCUSA Department of Orthopedic Surgery Duke University Medical CenterDurhamNCUSA

Skeletal muscle plays an essential role in generating the mechanical force necessary to support the movement of our body and daily exercise. Compared with cardiac and smooth muscle, in mammals, skeletal muscle exhibits remarkable regenerative capacity in response to damage. Muscle stem cells, also known as satellite cells, directly contribute to regeneration. Here, we review primary and secondary myogenesis processes with a focus on muscle stem cells, as well as the function and regulation of muscle stem cells in adult muscle regeneration in mammals.

关键词： muscle stem cell satellite cell skeletal muscle development skeletal muscle regeneration stem cell niche

激光定向能量沉积Inconel 718合金高频窄脉冲电化学后处理行为研究

在线全文

学校读者我要写书评

暂无评论

中国激光 2024年第10期51卷 255-266页

作者：耿建峰吴琳郭鹏飞王慧君林鑫张宇锋杨光黄卫东青岛理工大学山东增材制造工程技术研究中心山东青岛266520 西北工业大学凝固技术国家重点实验室陕西西安710072 沈阳航空航天大学机电工程学院辽宁沈阳110136

高频窄脉冲电解加工技术能有效提高加工精度和表面质量,在镍基高温合金等难加工金属材料的精密制造方面有着广泛的应用。然而,对于微观组织极不均匀的激光定向能量沉积构件,其加工质量尚不清晰,尤其是采用无水电解液时。以激光定向能量... 详细信息

高频窄脉冲电解加工技术能有效提高加工精度和表面质量,在镍基高温合金等难加工金属材料的精密制造方面有着广泛的应用。然而,对于微观组织极不均匀的激光定向能量沉积构件,其加工质量尚不清晰,尤其是采用无水电解液时。以激光定向能量沉积Inconel 718合金为研究对象,采用频率为30~100 kHz、占空比为30%~80%的高频窄脉冲电流以及饱和NaCl乙二醇电解液进行射流电解加工实验。结果表明:沉积态Inconel 718合金组织由γ基体相、Nb偏析区与枝晶间相(主要为γ/Laves共晶相)组成;在10.50 A/cm^(2)的电流密度下,加工区表面粗糙度随脉冲频率的增加而增大,且脉冲频率为30 kHz时表面粗糙度最小(R_(a)=1.562μm),加工精度最高;表面粗糙度随占空比的增加先减小后增大,占空比为50%时表面粗糙度最小,占空比为60%时加工精度最高;而在直流模式下,表面粗糙度随电流密度的增大而降低,且电流密度为10.50 A/cm^(2)时,表面质量最优(R_(a)=0.526μm),这是由于高电流密度更容易诱导表面“过饱和盐膜”的形成,从而有效抑制选择性溶解,降低表面粗糙度。但在加工精度方面,高频窄脉冲电流模式的加工定域性较好。最后,基于“盐膜”理论和双电层模型,揭示了高频窄脉冲电流模式下沉积态Inconel 718合金的微区阳极溶解机理,为提高激光增材制造镍基高温合金射流电解加工表面质量提供了理论支撑和实验依据。

关键词：激光技术激光定向能量沉积 Inconel 718镍基高温合金射流电解加工表面完整性加工精度

SIMILITUDE ANALYSIS AND SIMPLIFIED MODELS FOR WATER WAVES INDUCED BY EXPLOSION

在线全文

学校读者我要写书评

暂无评论

Journal of Hydrodynamics 1991年第3期3卷 41-49页

作者： Guo-guang, Shen De-jun, Li xin, lin Jing-chang, Yang Tianjin Univ Tianjin China

The similitude analysis of water waves induced by an explosion are investigated based on the principle of dimensional analysis in this paper. From the mechanism of water waves generated by an explosion, two mechanical models, formation of an initial crater and evolution of a water-column are proposed. The numerical simulation is accomplished by using the MAC method. The results indicate the importance of determining an equivalent water column for the prediction of some essential wave factors. Compared with the 8 kg TNT explosion touching water surface, the size of equivalent water column is obtained.

关键词： Computers Applications Fluid Mechanics Hydrodynamics

Interception, degradation and contributions of terrestrial organic carbon obtained from lignin analysis in Wujiang River, southwest China

在线全文

学校读者我要写书评

暂无评论

Acta Geochimica 2021年第6期40卷 857-870页

作者： xin lin Yujie Wang Jinhua Zhang Ming Yang Xueping Chen Fushun Wang Jing Ma School of Environmental and Chemical Engineering Shanghai UniversityShanghai 200444China

Understanding the fate of terrestrial organic carbon(OC)in a cascade impoundment system is critical for recognizing the role of carbon sink for reservoirs.Surface sediments collected from eight cascade reservoirs across the Wujiang River,southwestern China,were analyzed for elemental and stable carbon isotopic(δ^13C)composition,and lignin phenols(∑8 andΛ8)to investigate the spatial distribution,contribution,origin and degradation of sedimentary terrestrial OC.The values of total organic carbon(TOC)and∑8 exhibited a remarkable reduction along the upstream-downstream transect suggesting the trapping effect of cascade-damming.A relatively broad range ofδ^13C(-26.61 to-25.54%,95%CI)and C/N(6.80-18.20)indicated mixed allochthonous/autochthonous OC sources in surface sediments.The quantitative simulation indicates that the OC of the sediments mainly was derived from terrestrial organic matter.Soilderived OC rather than C_(3)vascular plant-derived OC makes a major contribution to sedimentary terrestrial OC in reservoirs on karst terrain.As evidenced by lignin compositions andδ^13C,the predominant vascular plant origins of terrestrial OC along the Wujiang River are non-woody angiosperm C_(3)plants.The aged reservoirs showed a trend of increasing contribution of autochthonous OC,which potentially weaken the role of carbon sink for reservoirs.The relationship between runoff inputs,watershed area/water surface area ratios,and water residence time and Λ8 were explored,indicating the natural and anthropogenic influences on terrestrial OC remains very complex in a cascade-damming river.

关键词： Lignin phenols Terrestrial organic carbon Cascade impoundment

核酸条形码技术:扩展蛋白质-蛋白质相互作用检测通量的新方法

在线全文

学校读者我要写书评

暂无评论

中国生物化学与分子生物学报 2024年第3期40卷 281-294页

作者：李林鑫秦晓红米立志天津大学生命科学学院结构与分子生物学系天津300072

蛋白质-蛋白质相互作用(protein-protein interaction,PPI)几乎参与了机体内所有重要的生物学过程,在细胞的基本生命过程中扮演了至关重要的角色,开发高通量的PPI检测新方法具有重要的生物学意义。目前,下一代测序技术(next-generation ... 详细信息

蛋白质-蛋白质相互作用(protein-protein interaction,PPI)几乎参与了机体内所有重要的生物学过程,在细胞的基本生命过程中扮演了至关重要的角色,开发高通量的PPI检测新方法具有重要的生物学意义。目前,下一代测序技术(next-generation sequencing,NGS)发展快速,能在几天内测定超过10亿个模板的DNA序列。由于并行DNA测序技术所特有的敏感性、特异性、高通量和多路复用优势,其已被用作广谱分子计数器,应用于基因组测序和转录物组测序等领域。核酸条形码技术通过将寡核苷酸标签与目标蛋白质连接起来,从而标记编码蛋白质。之后,利用高通量的测序方法检测相互作用的蛋白质,实现了PPI的高通量检测。这一技术推动了PPI检测方法的飞速发展,提升了单次实验检测的通量,为构建PPI网络提供了强有力的技术支持。本文详细阐述了核酸条形码在PPI检测方法中的设计、生成和读取;通过分析核酸条形码技术在PPI研究中的应用范例,探讨了各自的优势和不足,并评估了数据的可靠性,讨论了基于核酸条形码技术的PPI检测方法未来的发展趋势。

关键词：蛋白质-蛋白质相互作用核酸条形码检测技术下一代测序技术

Laser-based directed energy deposition of novel Sc/Zr-modified Al-Mg alloys:columnar-to-equiaxed transition and aging hardening behavior

在线全文

学校读者我要写书评

暂无评论

Journal of Materials Science & Technology 2021年第10期69卷 168-179页

作者： Zihong Wang xin lin Yao Tang Nan Kang Xuehao Gao Shuoqing Shi Weidong Huang State Key Laboratory of Solidification Processing Northwestern Polytechnical UniversityXi'an 710072China Key Laboratory of Metal High Performance Additive Manufacturing and Innovative Design MIIT ChinaNorthwestern Polytechnical UniversityXi'an 710072China

The control of grain morphology is important in laser additive manufacturing(LAM),as grain morphology further affects the hot cracking resistance,anisotropy,and strength–ductility synergy of materials.To develop a solidification-control solution and achieve columnar-to-equiaxed transition(CET)in Al-based alloys during LAM,Sc-and-Zr-modified Al-Mg alloys were processed via directed energy deposition(DED).CET was achieved by introducing high potent primary Al_(3)(Sc,Zr)nucleation sites ahead of the solidification interface.Furthermore,the relationship between the solidification control parameters and precipitation behavior of primary Al_(3)(Sc,Zr)nucleation sites was established using the time-dependent nucleation theory.Then,the CET was studied according to the Hunt criterion.The results indicated that coarse columnar grain structure was still obtained at the inner region of the molten pool at low Sc/Zr contents owing to the effective suppression of the precipitation of the primary Al_(3)(Sc,Zr)nucleation sites via rapid solidification during DED.In addition,the relatively low melt temperature at the fusion boundary unavoidably promoted the precipitation of primary Al_(3)(Sc,Zr)nucleation sites,which resulted in a fine equiaxed grains band at the edge of the molten pool.As the Sc/Zr content increased,the solidification cooling rate was not sufficient to suppress the precipitation of the primary Al_(3)(Sc,Zr)nucleation sites,and a fully equiaxed grain structure was obtained.Furthermore,the effect of the layer-by-layer manufacturing process on the subsequent precipitation strengthening of secondary Al_(3)(Sc,Zr)precipitates was discussed.Both the remelting and subsequent aging during thermal cycling should be considered to achieve greater precipitation strengthening.

关键词： Al-Mg-Sc-Zr alloys Additive manufacturing Directed energy deposition Columnar-to-equiaxed transition Phase precipitation

X-Paste improves wound healing in diabetes via NF-E2-related factor/HO-1 signaling pathway

在线全文

学校读者我要写书评

暂无评论

World Journal of Diabetes 2024年第6期15卷 1299-1316页

作者： Ming-Wei Du xin-lin Zhu Dong-xing Zhang Xian-Zhen Chen Li-Hua Yang Jin-Zhou Xiao Wen-Jie Fang Xiao-Chun Xue Wei-Hua Pan Wan-Qing Liao Tao Yang Institute of Cardiovascular Disease Shanghai Key Laboratory of Traditional Chinese Clinical MedicineShanghai University of Traditional Chinese MedicineShanghai 200120China Department of Dermatology Shanghai Key Laboratory of Molecular Medical MycologySecond Affiliated Hospital of Naval Medical UniversityShanghai 200003China Department of Dermatology Dongshan HospitalMeizhou 514000Guangdong ProvinceChina Department of Dermatology Central Hospital Affiliated to Shandong First Medical UniversityJinan 250000Shandong ProvinceChina Department of Emergency Naval Hospital of Eastern TheaterZhoushan 316000Zhejiang ProvinceChina Department of Pharmacy 905th Hospital of People’s Liberation Army of China(PLA)NavyShanghai 200052China

BACKGROUND Diabetic foot ulcers(DFU),as severe complications of diabetes mellitus(DM),significantly compromise patient health and carry risks of amputation and mortality.AIM To offer new insights into the occurrence and development of DFU,focusing on the therapeutic mechanisms of X-Paste(XP)of wound healing in diabetic mice.METHODS Employing traditional Chinese medicine ointment preparation methods,XP combines various medicinal ingredients.High-performance liquid chromatography(HPLC)identified XP’s main components.Using streptozotocin(STZ)-induced diabetic,we aimed to investigate whether XP participated in the process of diabetic wound healing.RNA-sequencing analyzed gene expression differences between XP-treated and control groups.Molecular docking clarified XP’s treatment mechanisms for diabetic wound healing.Human umbilical vein endothelial cells(HUVECs)were used to investigate the effects of Andrographolide(Andro)on cell viability,reactive oxygen species generation,apoptosis,proliferation,and metastasis in vitro following exposure to high glucose(HG),while NF-E2-related factor-2(Nrf2)knockdown elucidated Andro’s molecular mechanisms.RESULTS XP notably enhanced wound healing in mice,expediting the healing process.RNA-sequencing revealed Nrf2 upregulation in DM tissues following XP treatment.HPLC identified 21 primary XP components,with Andro exhibiting strong Nrf2 binding.Andro mitigated HG-induced HUVECs proliferation,metastasis,angiogenic injury,and inflammation inhibition.Andro alleviates HG-induced HUVECs damage through Nrf2/HO-1 pathway activation,with Nrf2 knockdown reducing Andro’s proliferative and endothelial protective effects.CONCLUSION XP significantly promotes wound healing in STZ-induced diabetic models.As XP’s key component,Andro activates the Nrf2/HO-1 signaling pathway,enhancing cell proliferation,tubule formation,and inflammation reduction.

关键词： Words:Diabetes mellitus Wound healing NF-E2-related factor-2/HO-1 signaling pathway Andrographolide