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含肝细胞生长因子水凝胶修复家兔股骨颈部缺损

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中国组织工程研究 2014年第52期18卷 8470-8473页

作者：郭林甄瑞鑫赵德伟田丰德于晶朱瑞萍大连大学附属中山医院辽宁省大连市116001

背景:肝细胞生长因子可促进骨组织再生,在骨组织修复方面有着巨大的潜力,但是在体内较短的半衰期限制了其在临床的应用。目的:观察含有肝细胞生长因子的半合成细胞外基质样水凝胶体内促进股骨颈骨缺损修复的作用。方法:取12只兔制作双... 详细信息

背景:肝细胞生长因子可促进骨组织再生,在骨组织修复方面有着巨大的潜力,但是在体内较短的半衰期限制了其在临床的应用。目的:观察含有肝细胞生长因子的半合成细胞外基质样水凝胶体内促进股骨颈骨缺损修复的作用。方法:取12只兔制作双侧股骨颈骨缺损模型,采用自体配对对比,左侧为对照侧不对骨缺损做任何处置,右侧为实验侧植入含有肝细胞生长因子的半合成细胞外基质样水凝胶。结果与结论:苏木精-伊红染色见2周时实验侧缺损修复区血管分布较对照侧均匀;4周时实验侧缺损区均匀填充新生骨小梁,而对照侧由外向内骨小梁形成减少;8周时实验侧缺损区皮质骨形成,并且骨髓腔再通,有骨髓细胞填充,对照侧仍有粗大骨痂填充缺损区,堵塞髓腔。钼靶X射线观测到8周时,实验侧难以区分正常骨质与新生骨质交界线,而对照侧缺损区与周围骨质分界明显。证实含有肝细胞生长因子的半合成细胞外基质样水凝胶可以促进家兔股骨颈部骨缺损的骨组织修复。

关键词：组织构建缓释材料肝细胞生长因子细胞外基质材料组织工程水凝胶骨缺损修复兔半合成细胞外基质样水凝胶

8种木本植物木质部栓塞变化与生理生态指标间关系的研究(II)——与光合生理生态指标的关系

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西北林学院学报 2006年第1期21卷 37-42页

作者：安锋蔡靖姜在民张远迎赵平娟张硕新西北农林科技大学林学院西北农林科技大学生命学院

选取西北农林科技大学西林校区校园内自然状况下生长良好的耐旱树种刺槐(Robin iap seudoacacia)、元宝枫(A cer truncatum)、沙棘(H ippophae rhamnoid es)、白榆(U lmus pum ila)、油松(P inus tabu laeform is)、白皮松(P inus bungeana)及中生树种女贞(L igustrum lucidum)、柳树(S a lix m atsud ana K o *** la)为研究对象,测定了它们3个季节内的木质部栓塞日变化规律及其与光照强度、大气温度、气孔导度、蒸腾速率等生理生态指标的关系。结果表明:光照强度、大气湿度和大气温度主要是通过对植物气孔运动、蒸腾作用和体内水分关系的调节对木质部栓塞起作用的;木质部栓塞是植物一天生长过程中不可避免的“平常事件”,与植物的光合速率、蒸腾速率、气孔导度和水分利用效率间存在一定的相关关系;它的发生和恢复可能是某些植物适应水分胁迫的一种机制。

关键词：木质部栓塞生态指标生理指标

全血金标免疫渗滤法快速检测棘球蚴病的现场应用

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中国寄生虫学与寄生虫病杂志 2005年第2期23卷 90-92页

作者：陈新华温浩张朝霞冯晓辉张静萍张金辉马旭东郑树森浙江大学医学院附属第一医院肝胆胰外科杭州310031 新疆维吾尔自治区包虫病临床研究所新疆包虫病基础医学重点实验室乌鲁木齐830054 新疆维吾尔自治区临床检验中心乌鲁木齐830000

目的建立一种检测全血标本的棘球蚴病现场快速诊断方法。方法制备检测棘球蚴病抗体的全血金标免疫渗滤法(DIGFA)快速诊断试剂盒,以土豆凝集素与全血混合达到快速凝血的目的,并对1678名流行区健康体检者、38例棘球蚴病患者、52名非流行... 详细信息

目的建立一种检测全血标本的棘球蚴病现场快速诊断方法。方法制备检测棘球蚴病抗体的全血金标免疫渗滤法(DIGFA)快速诊断试剂盒,以土豆凝集素与全血混合达到快速凝血的目的,并对1678名流行区健康体检者、38例棘球蚴病患者、52名非流行区健康体检者、40例其他肝肺占位患者做棘球蚴病全血现场诊断试验,评价其作为初筛棘球蚴病普查手段的实际应用价值。结果全血DIGFA对1678份流行区体检者阳性检出率为8.46%,其中肝脏B超及胸部透视阳性检出率为3.04%,对比免疫学检查142例阳性结果和影像学检查51例阳性结果:影像学检查阳性51例中有43例全血DIGFA为阳性,对其余8例影像学检查阳性而全血DIGFA阴性体检者进行16个月随访,经CT检查或病理学检查证实为肝棘球蚴坏死(3例)、肝钙化(2例)、肝囊肿(2例)、肝癌(1例)。而免疫学检查阳性的142例体检者中43例B超为阳性,对其余99例全血DIGFA阳性而影像学检查阴性的体检者进行16个月随访,发现肺部棘球蚴3例(随访仍在继续,数据统计截止到2003年6月)。对临床手术和病理检查确诊的38例棘球蚴病患者的阳性检出率为89.5%,对52份非流行区无狗羊接触史的健康体检者全血血样阳性检出率为0,与40例其他非棘球蚴性肝肺占位疾病(肝囊肿10例、肝血管瘤10例、肝癌10例、肺结核6例、肺癌4例)全血血样无交叉反应。随机抽取各组全血标本190例分别提取全血和血清标本,进行全血DIGFA、血清DIGFA和血清ELISA单盲法检测,三者检测结果差异无显著性(P>0.05)。结论棘球蚴病全血快速诊断试剂盒操作简便和快速,适合普查和流行病学调查。

关键词：金标免疫渗滤法棘球蚴病现场应用快速检测全血快速诊断试剂盒 DIGFA 阳性检出率影像学检查健康体检者 2003年6月免疫学检查快速诊断方法流行病学调查全血标本非流行区阳性结果病理学检查 ELISA 诊断试验应用价值

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大鼠骨髓间充质干细胞磁标记及MR成像研究

中华放射学杂志 2006年第2期40卷 155-159页

作者：蔡金华冯敢生王新吴汉平赵建农郭大靖余国容黎川刘官信王世一华中科技大学同济医学院附属协和医院放射科武汉430022 第三军医大学附属西南医院放射科重庆医科大学附属第二医院放射科重庆医科大学附属儿童医院放射科重庆医科大学附属儿童医院儿科研究所

目的应用菲立磁-多聚左旋赖氨酸复合物标记大鼠骨髓间充质干细胞,探讨MR成像显示磁标记干细胞的可行性。方法制备菲立磁-多聚左旋赖氨酸复合物。分离培养Wistar大鼠骨髓间充质干细胞,以菲立磁-多聚左旋赖氨酸复合物标记干细胞。分别于... 详细信息

目的应用菲立磁-多聚左旋赖氨酸复合物标记大鼠骨髓间充质干细胞,探讨MR成像显示磁标记干细胞的可行性。方法制备菲立磁-多聚左旋赖氨酸复合物。分离培养Wistar大鼠骨髓间充质干细胞,以菲立磁-多聚左旋赖氨酸复合物标记干细胞。分别于标记后24h及1、2、3周行普鲁士蓝染色观察细胞内铁,台盼蓝排除试验检测细胞活力。应用1.5TMR仪,以SE序列T1WI、T2WI和梯度回波(GRE)序列T2WI行磁标记干细胞成像。结果普鲁士蓝染色显示细胞质内大量铁颗粒存在,标记率100%;随细胞分裂增殖,细胞内铁颗粒逐渐减少。干细胞磁标记后24h及1、2、3周的台盼蓝拒染率分别为91.00%、93.00%、91.75%和92.50%,与未标记细胞相比较差异无统计学意义(P>0.05)。103、104、105个磁标记干细胞T2WI信号降低分别为63.75%、82.31%、91.92%,T2WI信号降低分别为68.24%、83.01%、93.94%。105个干细胞磁标记后24h及1、2、3周T2WI信号降低分别为93.75%、75.92%、41.75%、8.83%。结论应用菲立磁-多聚左旋赖氨酸复合物标记大鼠间充质干细胞安全、有效;T2WI对磁标记干细胞的显示最敏感;MR信号改变与干细胞数目及分裂增殖状态相关。

关键词：干细胞磁共振成像诊断显像动物实验

经尿道等离子前列腺分离电切术和传统电切术治疗BPH的疗效比较

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中华男科学杂志 2011年第5期17卷 435-439页

作者：赵国栋杨景玲刘致中王登宇高文峰李建新刘建平陈勇包头第八医院泌尿外科内蒙古包头014040 包钢医院泌尿外科内蒙古包头014040

目的:比较经尿道等离子前列腺分离电切术和等离子刀经尿道传统电切术治疗良性前列腺增生(BPH)的临床疗效,并对分离电切术进行研究。方法:2005年9月至2010年8月,收集81例BPH患者,随机分为2组。单盲法,行经尿道等离子前列腺分离电切术40例... 详细信息

目的:比较经尿道等离子前列腺分离电切术和等离子刀经尿道传统电切术治疗良性前列腺增生(BPH)的临床疗效,并对分离电切术进行研究。方法:2005年9月至2010年8月,收集81例BPH患者,随机分为2组。单盲法,行经尿道等离子前列腺分离电切术40例,行等离子刀经尿道传统电切术41例;比较2组年龄、术前超声测量前列腺体积、手术中切除腺体重量、手术时间、术中出血、术后带尿管时间、术前及术后IPSS评分(国际前列腺症状评分)。结果:两组病例仅术后IPSS评分比较有统计学意义(P<0.05),分离电切组与传统电切组IPSS评分分别为(8.70±1.13)分和(9.95±1.54)分。结论:经尿道等离子前列腺分离电切术和等离子刀经尿道传统电切术比较,经尿道等离子前列腺分离电切术临床疗效更为显著。

关键词：良性前列腺增生经尿道等离子前列腺分离电切术等离子刀经尿道传统电切术

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T大颗粒淋巴细胞白血病临床及实验室特征

中华血液学杂志 2009年第3期30卷 179-182页

作者：赵馨周康王慧君张莉刘庆国井丽萍李洪强杨栋林储榆林张凤奎中国医学科学院、北京协和医学院血液学研究所、血液病医院天津300020

目的提高对T大颗粒淋巴细胞白血病（T—LGLL）的认识。方法回顾性分析我院1999年6月至2007年7月间确诊的27例T—LGLL患者临床及实验室检查特征。结果T—LGLL起病潜隐、进展缓慢，中位确诊年龄48岁。贫血相关症状最为突出，51．9％患者... 详细信息

目的提高对T大颗粒淋巴细胞白血病（T—LGLL）的认识。方法回顾性分析我院1999年6月至2007年7月间确诊的27例T—LGLL患者临床及实验室检查特征。结果T—LGLL起病潜隐、进展缓慢，中位确诊年龄48岁。贫血相关症状最为突出，51．9％患者脾脏轻中度肿大，14．8％患者肝脏轻度肿大。未有合并类风湿性关节炎者。外周血中性粒细胞〈1．5×10^9／L者19例，占70．4％，〈0．5×10^9／L者5例，占18．5％。贫血占88．9％，中位血红蛋白58g/L，合并纯红细胞再生障碍者18例，占66．67％。外周血大颗粒淋巴细胞（LGL）绝对值中位数1．45×10^9／L，21例（77．8％）患者LGL数≤2．0×10^9／L，22例（81．5％）患者LGL免疫表型为CD3^＋CD8^＋CD57^＋CD56^＋。免疫抑制治疗总有效率91．3％，完全血液学缓解率为65．2％。结论T—LGLL起病及进展缓慢，主要表现为血细胞减少和脾脏肿大，常合并纯红细胞再生障碍。多数患者外周血LGL计数≤2．0×10^9／L，免疫表型以CD3^＋CD8^＋CD57^＋为主，对免疫抑制治疗反应良好。

关键词：白血病大颗粒淋巴细胞贫血再生障碍纯红细胞免疫抑制治疗

Extension Protocol for the Investigation on Nutrition Status and Clinical Outcome of Patients with Common Cancers in China(INSCOC)Study:2021 update

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Journal of Nutritional Oncology 2022年第2期7卷 64-74页

作者： Hong Xia Xu Chun Hua Song Liang yu Yin Chang Wang Zhen Ming Fu Zeng Qing Guo yuan Lin Ying Ying Shi Wen Hu Yi Ba Su Yi Li Zeng Ning Li Kun Hua Wang jing Wu Ying He Jia Jun Yang Cong Hua Xie Fu Xiang Zhou xin Xia Song Gong Yan chen Wen Jun Ma Su Xia Luo Zi Hua chen Zhi Kang chen Ming Hua Cong Hu Ma Chun Ling Zhou Wei Wang Qi Luo Yong Mei Shi yu Mei Qi Hai ping Jiang Wen Xian Guan Jun Qiang chen Xiang Hua Wu Jia xin chen yu Fang Lan Zhou Yong Dong feng Rong Shao Tan Tao Li Jun Wen Ou Qing Chuan zhao Jian Xiong Wu Min Weng Qing Hua Yao Ya Yin yu Quan Jun Lyu Hong Qiu Ming Liu Wei Li Han ping Shi The Investigation on Nutrition Status and Clinical Outcome of Common Cancers(INSCOC)Group Department of Nutrition Daping HospitalArmy Medical University(Third Military Medical University)Chongqing 400042China Department of Epidemiology College of Public HealthZhengzhou UniversityZhengzhou 450001HenanChina Cancer Center of the First Hospital of Jilin University Changchun 130021Jilin China Cancer Center Renmin Hospital of Wuhan UniversityWuhan 430060HubeiChina Department of Medical Oncology Fujian Cancer HospitalFujian Medical University Cancer HospitalFuzhou 350014FujianChina Department of Gastrointestinal Surgery Affiliated Tumor Hospital of Guangxi Medical UniversityNanning 530021GuangxiChina Department of Surgery The First Affiliated Hospital of SunYat-Sen UniversityGuangzhou 510080GuangdongChina Department of Clinical Nutrition West China Hospital of Sichuan UniversityChengdu 610041SichuanChina Department of Gastrointestinal Oncology Tianjin Medical University Cancer Institute and HospitalNational Clinical Research Center for CancerTianjin Key Laboratory of Cancer Prevention and TherapyTianjin 300060China Department of Nutrition and Metabolism of Oncology The First Affiliated Hospital of University of Science and Technology of ChinaAnhui Provincial Cancer HospitalHefei 230031AnhuiChina Department of Clinical Nutrition The First Hospital of Hebei Medical UniversityShijiazhuang 050031HebeiChina Department of Gastrointestinal Surgery Institute of GastroenterologyThe First Affiliated Hospital of Kunming Medical UniversityKunming 650032YunnanChina Department of Clinical Nutrition The First Affiliated Hospital of Zhejiang Chinese Medical UniversityZhejiang Provincial Hospital of Traditional Chinese MedicineHangzhou 310006ZhejiangChina Department of Clinical Nutrition Chongqing General HospitalChongqing 400014China Department of Colorectal and Anal Surgery Huizhou Municipal Central HospitalHuizhou 516001GuangdongChina Department of Radiation and Medical Oncology Zhongnan Hospital of Wuhan UniversityWuhan 430071HubeiChina Department of Oncology Xingtai Peo

Background Malnutrition is common in patients with cancer,and this adversely affects the survival and quality of life of *** Society for Nutritional Oncology issued a multi-center,large-scale,long-term follow-up prospective study,the Investigation on Nutrition Status and Clinical Outcome of Patients with Common Cancers in China(INSCOC study)since *** is an extension to the previous 2013-2020 study *** study still sought to:①address the prognostic impact of nutritional factors and quality of life on cancer patient survival;②describe the overall and cancer-specific incidence and/or distribution of malnutrition and different measurements of patient quality of *** and study design This is an observational,multi-centered,hospital-based prospective cohort *** collection will be performed at baseline(within 48 hours after patient admission),during the hospital stay and 30 days after hospital ***-up will be conducted for 1-20 years after *** primary outcome will be the all-cause mortality/overall survival,and secondary outcomes will be the length of hospital stay and costs of *** factors will include demographic characteristics,tumor characteristics,information about chronic diseases,hematological measurements(e.g.,red blood cell count,total lymphocyte counts,hemoglobin,albumin,prealbumin,creatinine,C-reactive protein,IL-6),anthropometric measurements(e.g.,height,weight,arm circumference,arm muscle circumference,triceps skinfold thickness,and waist circumference),body composition parameters,PG-SGA scores,quality of life(as indicated by the QLQ-C30 questionnaire),muscle mass(as indicated by the calf circumference),muscle strength(as indicated by the handgrip strength),muscle function(as indicated by the six-meter walking speed test)and physical status assessments(as indicated by the Karnofsky Performance Status scores).This clinical study protocol was approved by local Ethics Committees of

关键词： Cancer Malnutrition Nutritional status Nutritional assessment Survival Patient-Generated Subjective Global Assessment

蜂王浆中链霉素残留的提取、净化及液相色谱测定方法研究

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食品科学 2008年第11期29卷 487-489页

作者：薛晓锋吴黎明陈兰珍李桂芬黄京平李熠赵静中国农业科学院蜜蜂研究所北京100093

本实验对蜂王浆中链霉素残留的提取净化及液相色谱检测方法进行研究。蜂王浆样品用含有蛋白沉淀剂的磷酸提取液(pH2.0)溶解,超声波辅助提取蜂王浆中的链霉素残留。样液经离心后,分别用阳离子交换柱和反相固相萃取柱净化。采用柱后衍生-... 详细信息

本实验对蜂王浆中链霉素残留的提取净化及液相色谱检测方法进行研究。蜂王浆样品用含有蛋白沉淀剂的磷酸提取液(pH2.0)溶解,超声波辅助提取蜂王浆中的链霉素残留。样液经离心后,分别用阳离子交换柱和反相固相萃取柱净化。采用柱后衍生-高效液相色谱梯度洗脱与荧光检测分析蜂王浆中的链霉素残留。以Atlantis HILIC Silica(2.1mm×150mm,3.0μm)为色谱柱,以酸性水溶液和乙腈为流动相,链霉素在25min内实现较好的分离,方法快速准确,灵敏度高,是一种较好的定性和定量方法。所研究的提取净化方法及色谱分离条件能有效排除蜂王浆中的杂质干扰,添加回收率在71.7%～77.4%之间,方法检出限为10μg/kg。

关键词：蜂王浆链霉素高效液相色谱荧光检测器柱后衍生

Validity of the Chinese Version of the Patient-Generated Subjective Global Assessment (PG-SGA) in Gastric Cancer Patients

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Journal of Nutritional Oncology 2018年第4期3卷 182-188页

作者： Zhen Ming Fu Hong Xia Xu Chun Hua Song Wei Li Zeng Qing Guo yuan Lin Ying Ying Shi Wen Hu Yi Ba Su Yi Li Zeng Ning Li Kun Hua Wang jing Wu Ying He Jia Jun Yang Cong Hua Xie xin Xia Song Gong Yan chen Wen Jun Ma Su Xia Luo Zi Hua chen Ming Hua Cong Hu Ma Chun Ling Zhou Wei Wang Qi Luo Yong Mei Shi yu Mei Qi Hai ping Jiang Wen Xian Guan Jun Qiang chen Jia xin chen yu Fang Lan Zhou Yong Dong feng Rong Shao Tan Tao Li Jun Wen Ou Qing Chuan zhao Jian Xiong Wu Chang Wang Han ping Shi The Investigation on Nutrition Status and Clinical Outcome of Common Cancers (INSCOC) Group Cancer Center Renmin Hospital of Wuhan University Wuhan Hubei 430060 China Department of Nutrition and Metabolism of Oncology Affiliated Provincial Hospital of Anhui Medical University Hefei Anhui 230031 China Department of Clinical Nutrition The First Hospital of Hebei Medical University Shijiazhuang Hebei 050031 China Department of Gastrointestinal Surgery Institute of Gastroenterology The First Affiliated Hospital of Kunming Medical University Kunming Yunnan 650032 China Department of Clinical Nutrition The First People's Hospital of Kashi Xinjiang 844000 China Department of Clinical Nutrition Chongqing General Hospital Chongqing 400014 China Department of Colorectal and Anal Surgery Huizhou Municipal Central Hospital Huizhou Guangdong 516001 China Department of Radiation and Medical Oncology Zhongnan Hospital of Wuhan University Wuhan Hubei 430071 China Department of Oncology Xingtai People’s Hospital Hebei Medical University Xingtai Hebei 054031 China The First Department of the Tumor Hospital of Harbin Medical University Harbin Heilongjiang 150085 China Department of Nutrition Guangdong General Hospital Guangdong Academy of Medical Sciences Guangzhou Guangdong 510080 China Department of Nutrition Daping Hospital & Research Institute of Surgery Third Military Medical University Chongqing 400042 China Department of Oncology Affiliated Cancer Hospital of Zhengzhou University and Henan Cancer Hospital Zhengzhou Henan 450008 China Department of General Surgery Xiangya Hospital Central South University Changsha Hunan 410008 China Comprehensive Oncology Department Cancer Hospital Chinese Academy of Medical Sciences Beijing 100021 China Department of Oncology Affiliated Hospital of Zunyi Medical University Zunyi Guizhou 563000 China The Fourth Affiliated Hospital Harbin Medical University Harbin Heilongjiang 150001 China Cancer center The First People's Hospital of Foshan Foshan Guangdong 528000 China Department of Gastrointestinal Tumor Surge

Purpose Malnutrition is severe among gastric cancer patients in China. While the Patient-Generated Subjective Global Assessment (PG-SGA) is a widely used nutritional assessment tool, the validation for Chinese patients with gastric cancer has not been performed yet. The aim of this research is to validate the Chinese version of the PGSGA in Chinese gastric cancer patients by conducting analyses of nutrition screening and assessment data. Methods Two thousand consecutive adult patients with gastric cancer were included in this observational, cross-sectional study in several tertiary hospitals in China. A survey was made among those patients, which included several questionnaires and some anthropometric parameters. We calculated the parameters to validate the use of PG-SGA categorizing the nutritional status of gastric cancer patients. Then we performed statistical analysis to evaluate the internal consistency, external consistency, and construct validity. Results This study verified the ability of the PG-SGA to differentiate gastric cancer patients into different nutritional groups. The confirmatory factor analysis verified the original four-factor structure of the PG-SGA. The internal and external consistency was measured by a concordance analysis which showed an intraclass correlation coefficients (ICC) of 0.987. The correlations of the Box 4 score with the Karnofsky performance status score demonstrated alternative form validity with good external consistency (Pearson, r = 0.643, P < 0.001). All three anthropometric dimensions of the PG-SGA were supported by principal component analysis. Spearman correlations (P < 0.001) were significant in the majority of tests. Conclusion The Chinese version of the PG-SGA is a valid tool to access the patients’ nutritional status for Chinese gastric patients.

关键词： Validation Nutrition status Chinese version Cancer