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Detecting Cryptosporidium parvum and Giardia lamblia by coagulation concentration and real-time PCR quantification

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Frontiers of Environmental Science & Engineering 2013年第1期7卷 49-54页

作者： Huining ZHANG Xiaohu Zhang Shuting ZHANG Bo WEI Qipei jiang xin YU Key Laboratory of Urban Environment and Health Institute of Urban Environment Chinese Academy of Sciences Xiamen 361021 China

Rapid and sensitive pathogen detection methods are essential for the effective functioning of the water treatment industry, yet for many pathogens, effective detection and removal methods are still lacking. Cryptosporidium parvum oocysts and Giardia lamblia cysts are two of the most common waterborne pathogens currently infecting the water supply. In this study, a new method was developed for the detection of Cryptosporidium parvum oocysts and Giardia lamblia cysts. The method includes multi-steps as coagulation concentration of (oo)cysts in water, the dissolution of the resulting flocs into a small volume using acid, filtration of the (oo)cysts solution, and DNA extraction, purification, and examination using real-time PCR. The method was tested using spiked tap water and reservoir water as references, and the mean recovery ranged from 19.6% to 97.6% for oocysts, and from 51.4% to 98.7% for cysts. The method is economical and convenient, and is especially suitable for relatively high turbidity surface water.

关键词： Cryptosporidium parvum detection method Giardia lamblia surface water

In Situ Controllable Growth of Cu_2SnS_3 Film as Low-Cost Counter Electrodes for Dye-Sensitized Solar Cells

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Acta Metallurgica Sinica(English Letters) 2015年第5期28卷 580-583页

作者： Wei Xu xin jiang Jian-Min Chen Rui-Qin Tan Wei-Jie Song Ningbo Institute of Material Technology and Engineering Chinese Academy of Sciences Ningbo Key Laboratory of Silicon and Organic Thin Film Optoelectronic Technologies State Key Laboratory of Solid Lubrication Lanzhou Institute of Chemical Physics Chinese Academy of Sciences Faculty of Information Science and Engineering Ningbo University

Morphology-controllable Cu2SnS3 thin films on solvothermal process and used in dye-sensitized solar cells as Mo-glass were prepared via a facile in situ one-step counter electrodes. The effects of different solvents on the morphology of films were investigated. DSC based on the porous net-like Cu2SnS3 thin film as counter electrodes showed a power conversion efficiency of 2.30%, which was improved to 3.35% after annealing.

关键词： Cu2SnS3 Thin films Counter electrodes Solar cells Power conversion efficiency

Multicenter evaluation of a simple and sensitive nucleic acid self-testing for SARS-CoV-2

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Virologica Sinica 2023年第4期38卷 620-626页

作者： xin-xin Shen Feng-Yu Li Meng Qin Guo-Hao Zhang Meng-Yi Zhang Hong Liu Xiu-Li Sun Zhen-jiang xin Xue-Jun Ma NHC Key Laboratory of Medical Virology and Viral Diseases National Institute for Viral Disease Control and PreventionChinese Center for Disease Control and PreventionBeijing102206China Hebei Medical University Shijiazhuang050031China Fengtai District Center for Disease Control and Prevention of Beijing Beijing100071China Beijing Baicare Biotechnology Co. Ltd.Beijing102206China Shandong University of Technology School of Life Sciences and MedicineZibo255000China

A rapid and accurate COVID-19 diagnosis is a prerequisite for blocking the source of infection as soon as possible and taking the appropriate medical ***,we developed GeneClick,a device for nucleic acid self-testing of SARS-CoV-2,consisting of three modules:a sampling kit,a microfluidic chip-based disposable cartridge,and an amplification *** addition,we evaluated the clinical performance of GeneClick using 2162 nasal swabs collected at three medical institutions,using three commercial RT-qPCR kits and an antigen self-test as *** to RT-qPCR,the sensitivity and specificity of the GeneClick assay were 97.93%and 99.72%,respectively,with a kappa value of 0.979(P<0.01).Of the 2162 samples,2076 were also tested for SARS-CoV-2 *** the 314 positive samples identified by GeneClick assay,63 samples were undetected by antigen ***,the GeneClick nucleic acid self-test demonstrated higher accuracy than the antigen-based *** on the additional features,including simple operation,affordable price,portable device,and reliability of smartphone APP-driven sampling and result reporting,GeneClick offers a powerful tool for field-based SARS-CoV-2 detection in primary healthcare institutions or at-home use.

关键词： Nucleic acid self-testing SARS-CoV-2 RT-qPCR Antigen-test

Use of Organic Solvents to Extract Organochlorine Pesticides (OCPs) from Aged Contaminated Soils

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Pedosphere 2013年第1期23卷 10-19页

作者： YE Mao YANG xing-Lun SUN Ming-Ming BIAN Yong-Rong WANG Fang GU Cheng-Gang WEI Hai-jiang SONG Yang WANG Lei JIN xin jiang xin State Key Laboratory of Soil and Sustainable Agriculture Institute of Soil Science Chinese Academy of Sciences

Problems associated with organochlorine pesticide （OCP）-contaminated sites in China have received wide attention. To solve such problems, innovative ex-situ methods of site remediation are urgently needed. We investigated the feasibility of the extraction method with different organic solvents, ethanol, 1-propanol, and three fractions of petroleum ether, using a soil collected from Wujiang （W J）, China, a region with long-term contamination of dichlorodiphenyltrichloroethanes （DDTs）. We evaluated different influential factors, including organic solvent concentration, washing time, mixing speed, solutiomto-soil ratio, and washing temperature, on the removal of DDTs from the WJ soil. A set of relatively better parameters were selected for extraction with 100 mL L-1 petroleum ether （60-90 ℃）： washing time of 180 min, mixing speed of 100 r min-I, solution-to-soil ratio of 10：1, and washing temperature of 50 ℃. These selected parameters were also applied on three other seriously OCP-polluted soils. Results demonstrated their broad-spectrum effectiveness and excellent OCP extraction performance on the contaminated soils with different characteristics.

关键词： dichlorodiphenyltrichloroethane ex-situ soil washing extraction performance petroleum ether site remediation

Soil heavy metal(loid)s and risk assessment in vicinity of a coal mining area from southwest Guizhou, China

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Journal of Central South University 2016年第9期23卷 2205-2213页

作者： QIN Fan-xin WEI Chao-fu ZHONG Shou-qin HUANG Xian-fei PANG Wen-pin jiang xin College of Resources and Environment Southwest University Chongqing 400716 China Key Laboratory for Information System of Mountainous Areas and Protection of Ecological Environment of Guizhou Province Guizhou Normal University Guiyang 550001 China

Total concentrations of arsenic, lead, cadmium, mercury, nickel, chromium, and copper in the soils from near a coal mine area in southwest Guizhou, China, were measured to evaluate the level of contamination, and the potential ecological risks posed by the heavy metals were quantitatively estimated. Results reveal that all heavy metals/metalloid exceeded the background values for soil environmental quality of heavy metals in Guizhou area. Geo-accumulation index(I_(geo)) showed that arsenic had the highest contamination level(I_(geo)=4) among the seven heavy metals/metalloid, and the contamination levels of mercury and lead were also relatively high(I_(geo)=3). Pearson correlation and cluster analysis identified that mercury, copper and arsenic had a relationship, and their presence might be mainly related to mining activity, coal and oil combustion, and vehicle emissions. Improved Nemerow index indicated that the overall level of heavy metal contamination in the studied area ranged from moderately–heavily contaminated to heavily contaminated level. Potential ecological risk index(R_I) analysis manifested that the whole ecological risk level ranged from high degree to very high degree(325.30≤R_I≤801.02) in the studied soil samples, and the potential ecological risk factors (E_r^i) of heavy metals/metalloid were as follows: Hg > As > Cd > Pb > Cu > Ni > Cr, and the E_r^i of Hg and As reached very high risk grade.

关键词： heavy metals soil contamination ecological risk coal mining area xingren county

Hoffa’s fracture in an adolescent treated with an innovative surgical procedure:A case report

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World Journal of Clinical Cases 2022年第4期10卷 1410-1416页

作者： Zu-xin jiang Pan Wang Shun-xin Ye Xiao-Ping Xie Chun-Xiu Wang Yue Wang Department of Orthopaedics No.2 People’s Hospital of Yibin CityYibin 644000Sichuan ProvinceChina Department of Oncology No.2 People’s Hospital of Yibin CityYibin 644000Sichuan ProvinceChina Department of Orthopedics Sichuan Provincial People's HospitalChengdu 640000Sichuan ProvinceChina

BACKGROUND Hoffa fracture is rare,especially in adolescents,and has a high rate of complications such as avascular necrosis and osteoarthritis;moreover,there are no definitive guidelines for its *** report could provide a new potential treatment for Hoffa *** SUMMARY A 16-year-old girl presented to the orthopedic emergency department of No.2 People’s Hospital of Yibin City with persistent pain following a right knee injury sustained during a sprint *** knee was swollen and tender,and the range of motion was restricted by the pain.X-ray and computed tomography revealed a Hoffa fracture in the right *** consultation,surgical treatment was performed,and the fracture was fixed with three 3.5-mm cannulated cancellous screws;osteochondral plugs that were harvested from the screw insertion site were re-implanted to cover the screw *** patient’s fracture and osteochondral plug healed 6 mo postoperatively,and she presented a knee range of motion of 0–135 without pain,and was walking without support with a normal *** Here,we describe an innovative surgical procedure for Hoffa fracture that could provide a new possibility for the treatment of similar fractures,and further improve their management.

关键词： Hoffa fracture Osteochondral plug Open reduction Internal fixation Case report

Study on the Topographic Effect on Soil Erosion Using RUSLE Model for Small Size Watershed

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水土保持研究 2006年第5期13卷 186-187,192页

作者： CHEN Chuan-sheng jiang xin Central South University of Forestry and Technology Changsha 410004 China The United Graduate School of Agriculture Sciences of Kagoshima University Kagoshima 890-0065 Japan

Soil erosion and subsequent sedimentation have caused serious environmental and soil degradation problems in Okinawa Prefecture,*** research aims at evaluating an availability of the Revised Universal Soil loss Equation（RUSLE） for predicting the range of soil loss values for the Nago watershed in *** shows that climatic conditions substantially influence the rainfall amount as a function of the I30 of the rainfall *** rate of soil loss is higher with increasing in altitude due to greater slope *** rainfall data analysis,it is concluded that the large difference in soil loss between 2000 and 2001 was due to concentrated heavy rainfall in the rainy season or the typhoon season.

关键词： RUSLE erosion index rainfall soil loss small size watershed

Bibliometric analysis of olaparib and pancreatic cancer from 2009 to 2022:A global perspective

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World Journal of Gastrointestinal Oncology 2024年第11期16卷 4489-4505页

作者： Xu Feng Yi-Han Chai Ke-xin jiang Wen-Bin jiang Wen-Chao Chen Yu Pan Department of General Surgery Sir Run Run Shaw HospitalSchool of MedicineZhejiang UniversityHangzhou 310016Zhejiang ProvinceChina

BACKGROUND Genetic screening for breast cancer gene 1(BRCA)1/2 mutations can inform breast/ovarian/pancreatic cancer patients of suitable therapeutic *** to seven percent of pancreatic cancer patients have germline BRCA *** genes aid in DNA repair,especially homologous recombination,which impacts genomic stability and cancer cell ***1 regulates the cell cycle,ubiquitination,and chromatin remodeling,whereas BRCA2 stimulates the immune *** predict the efficacy of platinum chemotherapy or polymerase(PARP)inhibitors such as *** To determine the trends and future directions in the use of olaparib for pancreatic cancer *** To evaluate the trends in how olaparib works in pancreatic cancer,we performed a bibliometric *** hundred and ninety-six related publications were accessed from the Web of Science Core Collection and were published between 2009 and *** analytic parameters included publications,related citations,productive countries and institutes,influential authors,and keyword *** This study visualizes and discusses the current research,including the present global trends and future directions in olaparib and pancreatic ***,this study sheds light on optimizing the use of olaparib in pancreatic cancer treatment,Feng X et *** use of olaparib in pancreatic cancer WJGO https://*** 4490 November 15,2024 Volume 16 Issue 11 offering valuable guidance for researchers in this *** Our findings identified trends in olaparib and pancreatic cancer,with China and the USA leading and with global cooperation tightening.O'Reilly EM's team and Memorial Sloan-Kettering had the highest *** Journal of Clinical Oncology was the most cited *** PARP inhibitors are emerging,and combination therapy is suggested for future therapeutic trends.

关键词： Olaparib Pancreatic cancer Bibliometric analysis Breast cancer susceptibility gene Poly(adenosine diphosphate-ribose)polymerase

Application of Tradable Emission Permits in Europe

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Chinese Journal of Population,Resources and Environment 2009年第2期7卷 71-75页

作者： jiang xin Feng Zongxian Zhao Chi Jinhe Center for Economic Research Xi＇an Jiaotong University Xi＇an Shaanxi 710049 China School of Economy and Finance Xi＇an Jiaotong University Xi＇an Shaanxi 710061 China

The purpose of this study is merely to review the current situation in the designing and implementation of the emission trading programs in Europe. Historical data show that although there is a series of shortcomings in their current functioning, employing such instruments for GHG reduction policy making is strongly expected to be efficient and effective. The European Union Emission Trading Scheme (EU ETS), Clean Development Mechanism (CDM) and Joint Implementation (JI) are just a few examples of the ambitious EU initiative that heavily relies on such instruments. We dwell on their operations and achievements by far and all the content in this article is expected to convince the Chinese government and regional public authorities to take positive actions and attitudes in promoting these instruments.

关键词： emission trading program market-based instrument EU ETS CDM JI