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The action of ethanol on G protein. <i>In silico</i>and cellular/molecular evidences

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Advances in Bioscience and Biotechnology 2013年第5期4卷 665-673页

作者： Pamela Fernandez Jessica Moreno Claudio Barrientos Sergio A. Aguila Daniela Leon Sebastián Ortiz Ramon Silva Francisco Rodriguez Maritza Leonardi Violeta Morin ximena Romo Departamento de Bioquimica y Biologia Molecular Facultad de Ciencias Biologicas Universidad de Concepción Concepcion Chile Departamento de Ciencias Biologicas Facultad de Ciencias Biologicas Universidad Andres Bello Sede Concepcion Talcahuano Chile Laboratorio de Virus Oncológicos en Reproducción BIOREN-CEGIN Universidad de La Frontera Temuco Chile Universidad Nacional Autonoma de Mexico Centro de Nanociencias y Nanotecnologia Ensenada Mexico

Ethanol (EtOH) enhances glycinergic currents in the central nervous system (CNS). Because evidence for an interaction between the α1 subunit of the glycine receptor (α1GlyR) and the G protein Gβγ subunit exists in vitro and because cAMP levels are known to increase in response to EtOH, we wanted to investigate the interaction between Gβγ and α1GlyR in response to EtOH treatment in HEK293 cells and to explore the possible sites of interaction between EtOH and the Gαs subunit. His pull-down assays in GlyR-His6-transfected HEK293 cells incubated with ethanol or propofol revealed that only EtOH treatment increased the binding of Gβγ heterodimers to α1GlyR. Using molecular modelling (protein structure prediction), was modelled the hGαs protein for the first time and validated this model by site-directed mutagenesis. By molecular docking, we identified some potential regions of interaction between hGαs and EtOH that are located on the SIII and SI regions of the Gαs. Therefore, we conclude that ethanol increases the interaction between α1GlyR and Gβγ in HEK293 cells, an effect that might be attributed to the interaction between EtOH and hGαs, which consequently stimulates hGαs.

关键词： Alcoholism Ethanol Glycine Receptor G Proteins Signals Transduction

Long-Term Impact in the Quality of Life of Patients with Drug-Resistant Epilepsy That Use Cannabidiol

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Neuroscience & Medicine 2021年第4期12卷 103-113页

作者： Gabriela Pesántez Ríos Ignacio Pascual Pascual ximena Pesántez Ríos Luciana Armijos Estefanía Chediak Hugo Navarrete Zambrano Ruth Jimbo Galo Pesantez Cuesta Servicio de Neurología Pediátrica Centro Nacional de Epilepsia Quito Ecuador Universidad Autónoma de Madrid Madrid Espa&#241 a Servicio de Neurología Pediátrica Hospital Universitario La Paz Madrid Espa&#241 a Centro de Investigación para la Salud en América Latina (CISeAL) Quito Ecuador Facultad de Medicina Pontificia Universidad Católica del Ecuador Quito Ecuador

Introduction: Epilepsy is considered a chronic neurological condition that manifests itself with seizures, where 30% - 40% of patients do not achieve control of their seizures despite proper management. Seizures represent a significant limitation in the patient’s daily activities and are often accompanied by emotional and relational difficulties that have a great impact on the quality of life of the patient and their families. Cannabidiol (CBD) has been found to be effective in controlling seizures and may also improve cognitive and behavioral abilities. Material and Methods: The Quality of Life of the Patient with Epilepsy (CAVE) scale was applied to patients with refractory epilepsies who use Cannabidiol (CBD) added to their base therapy, before the use of CBD and after 12 months of follow-up. The presentation of collateral effects was also evaluated. Results: Out of 34 patients, 26 (76.5%) increased their CAVE value at the end of the study and only 1 (2.9%) decreased. 19 (55.9%) improved in learning and behavior, 55.8% in the frequency of seizures and 79.4% reported a decrease in the intensity of seizures. There were other positive side effects such as improvement in alertness, language, sleep and behavior. The main side effects were mild and transitory, including drowsiness, and constipation. There was a correlation between the global perception of improvement and seizure control. Conclusions: This study shows that in the long term CBD improves the quality of life of patients with refractory epilepsies, through the control of seizures and the improvement of cognitive and behavioral functions.

关键词： Quality of Life Refractory Epilepsy Cannabidiol

Antioxidant activity of Bios-p peptide analogue in HEK293T cells and three-dimensional structure prediction

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Advances in Bioscience and Biotechnology 2013年第1期4卷 55-61页

作者： Pamela Fernández Manzano Ignacio Jofré Fernández Fabiola Zambrano Patricia Navarrete Gómez Magdalena Cuevas Troncoso ximena Romo Marty Andrés ávila Barrera Fernando Romero Mejía Center of Neurosciences and Peptides Biology Universidad de La Frontera Temuco Chile Centro de Modelación y Computación Científica Universidad de La Frontera Temuco Chile Departamento de Ciencias Biologicas Facultad de Ciencias Biologicas Universidad Andres Bello Talcahuano Chile

Studies had indicate that excessive production of reactive oxygen species (ROS) affect cellular signaling pathways, which is associated with pathological and physiological conditions such as cancer, diabetes and neurodegenerative diseases In this context, our laboratory has obtained the Bios-p, a ROS modulator, peptide analogue by sequencing from the seed of Bauhinia bauhinoides, which represents the active 12-amino acid, obtained from the inhibitor BbKI protease and we predicted the three-dimensional structure of Bios-p analogue peptide using homology modeling, being patented by the working group of Dr. Maria Luiza Vilela Oliva of UNIFESP, Brazil (a member of our cluster). The protective effect on the viability and antioxidant capacity of Bios-p was studied in HEK 293T cells under oxidative stress induced by hydrogen peroxide (H2O2) using SYTOXGREEN/DHE and luminescence assay. The three-dimensional structure of Bios-p peptide analogue was predicted by homology-based modeling using Modeller9v8. The pretreatment with different concentrations of Bios-p (1 μM - 10 μM) showed an increase of 53.83% ± 3.86% the cellular viability in under oxidative stress compared to control. Furthermore, the results to indicate that HEK293T cells by incubating for 24 h with Bios-p shown a significant decreased of basal extracellular ROS on total cell population in 89.67% ± 0.76%, compared to control in the absence of the analogue. Similarly it is observed that Bios-p has a significant antioxidant effect on extracellular ROS production when cells are subjected to oxidative stress induced by 200 μM H2O2 in 64.37% ± 4.63%, compared to control in absence of H2O2 and Bios-p. These results suggest that Bios-p has potential as antioxidant agent in cells HEK293T under H2O2-induced oxidative stress and that can protect the cells viability as concentration-dependent, and we propose a new biotechnological tool for modulate the ROS production.

关键词： Antioxidant Bios-p Bauhinia bauhinoides

Critically ill patients with cancer: A clinical perspective

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World Journal of Clinical Oncology 2020年第10期11卷 809-835页

作者： Frank Daniel Martos-Benítez Caridad de Dios Soler-Morejón Karla ximena Lara-Ponce Versis Orama-Requejo DailéBurgos-Aragüez Hilev Larrondo-Muguercia Rahim W Lespoir Intensive Care Unit 8B Hermanos Ameijeiras HospitalHavana 10300Cuba

Cancer patients account for 15%of all admissions to intensive care unit(ICU)and 5%will experience a critical illness resulting in ICU *** rates have decreased during the last decades because of new anticancer therapies and advanced organ support *** early critical care and organ support is associated with improved survival,timely identification of the onset of clinical signs indicating critical illness is crucial to avoid *** article focused on relevant and current information on epidemiology,diagnosis,and treatment of the main clinical disorders experienced by critically ill cancer patients.

关键词： Acute respiratory failure Cancer Cardiotoxicity Chemotherapy Critical care Infection Mechanical ventilation Neutropenia Postoperative Sepsis

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手术范围对膝关节术后纤维化形成的影响

中国骨伤 2018年第6期31卷 587-590页

作者：吴海啸 Karen A.Egiazaryan Andrei Р.Ratyev Guram D.Lazishvili ximena A.Sanchez Sanchez 张超俄罗斯联邦国立医科大学莫斯科俄罗斯1179972 俄罗斯圣彼得堡国立大学圣彼得堡俄罗斯199034 天津医科大学肿瘤医院骨与软组织肿瘤科天津300060

膝关节纤维化是术后常见的并发症,为影响手术疗效的关键因素之一,常引起关节活动度降低、慢性关节痛、软骨损伤以及关节废用性病变,严重降低了患者的生活质量。因此,如何降低术后膝关节纤维化的发生是提高手术预期疗效的关键,也是临床... 详细信息

膝关节纤维化是术后常见的并发症,为影响手术疗效的关键因素之一,常引起关节活动度降低、慢性关节痛、软骨损伤以及关节废用性病变,严重降低了患者的生活质量。因此,如何降低术后膝关节纤维化的发生是提高手术预期疗效的关键,也是临床医学上需要亟待解决的重要问题。笔者团队结合自身前期及最新的研究发现,膝关节纤维化的程度与手术范围大小有重要的关联,利用微创和关节镜方法治疗膝关节三室内的疾病可有效减少滑膜、软骨和脂肪垫组织的损伤,同时结合术后早期康复训练是未来预防术后膝关节纤维化的有效手段。

关键词：膝关节纤维化血管内皮生长因子外科手术

Seroprevalence study of infection with influenza A(H1N1)pdm09 virus in San Felipe Town, Chile

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World Journal of Cardiovascular Diseases 2013年第7期3卷 476-482页

作者： Olea Andrea Fasce Rodrigo Aguilera ximena Oliva Otavio Munoz Sergio Garcia Maritza Pedroni Elena Najera Manuel Guerrero Andrea Bustos Patricia Mora Judith Vega Jeanette Cerda Jaime Abarca Katia McCarron Meg Widdowson Marc-Alain Castillo Ernesto Alvarado Sergio Quijada Sandra Gallegos Doris Gonzalez Claudia Center of Epidemiology and Health Policies Department Faculty of MedicineUniversidad del DesarrolloConcepcionChile Section of Respiratory and Exanthematous viruses Sub-Department of Clinical VirologyInstitute of Public HealthMinistry of HealthSantiagoChile Center of Epidemiology and Health Policies of the Universidad del Desarrollo ConcepcionChile Panamerican Health Organization Washington DCUSA Public Health Department and Pediatrics Division Faculty of MedicinePontifical Catholic University of ChileSantiagoChile Centers for Diseases Control and Prevention AtlantaUSA Center for Micro Data Economics DepartmentUniversity of ChileSantiagoChile Epidemiology and Health Policies Department Universidad del DesarrolloConcepcionChile

Objective: To know the natural history of the first wave of pandemic influenza A(H1N1)pdm09 in the Southern hemisphere, through the detection of antibodies against influenza A(H1N1)pdm09 in a selected community, to estimate the population attack rate and its variations, the consultation rates, hospitallization and mortality rates. Methodology: A representative random sample of the population of a commune in Chile (San Felipe) was interviewed and taken blood samples between January and March 2010. A study against the antibodies of the influenza A(H1N1) pdm09 virus was conducted, by the technique of the Hemaglutination Inhibition (HAI) according to standardized methodology. Subjects with antibody titers ≥1:40 were considered positive. Results: 13.5% of the population of San Felipe had antibodies against influenza A(H1N1)pdm09;this percentage reached 30% of the population between 0 and 18 years and 6.1% among those over 19 years. The age variable was the only factor that evidenced significant differences in the prevalence of antibodies. There were no significant differences related to gender, vaccination history against seasonal inluenza, or comorbidity. 51% of people with positive serology showed IN-FLUENZA-LIKE SYMPTOMS. Conclusions: A relevant percentage of subclinical disease was detected in the first pandemic wave in Chile and the proportion of people with SARI and deaths was small. Data from epidemiological surveillance were useful to estimate the trend of TSI but not its magnitude.

关键词： Influenza A(H1N1)pdm09 Influenza Seroprevalence