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The action of ethanol on G protein. <i>In silico</i>and cellular/molecular evidences

Advances in Bioscience and Biotechnology 2013年第5期4卷 665-673页

作者： Pamela Fernandez Jessica Moreno Claudio Barrientos Sergio A. Aguila Daniela Leon Sebastián Ortiz Ramon Silva Francisco Rodriguez Maritza Leonardi Violeta Morin ximena Romo Departamento de Bioquimica y Biologia Molecular Facultad de Ciencias Biologicas Universidad de Concepción Concepcion Chile Departamento de Ciencias Biologicas Facultad de Ciencias Biologicas Universidad Andres Bello Sede Concepcion Talcahuano Chile Laboratorio de Virus Oncológicos en Reproducción BIOREN-CEGIN Universidad de La Frontera Temuco Chile Universidad Nacional Autonoma de Mexico Centro de Nanociencias y Nanotecnologia Ensenada Mexico

Ethanol (EtOH) enhances glycinergic currents in the central nervous system (CNS). Because evidence for an interaction between the α1 subunit of the glycine receptor (α1GlyR) and the G protein Gβγ subunit exists in vitro and because cAMP levels are known to increase in response to EtOH, we wanted to investigate the interaction between Gβγ and α1GlyR in response to EtOH treatment in HEK293 cells and to explore the possible sites of interaction between EtOH and the Gαs subunit. His pull-down assays in GlyR-His6-transfected HEK293 cells incubated with ethanol or propofol revealed that only EtOH treatment increased the binding of Gβγ heterodimers to α1GlyR. Using molecular modelling (protein structure prediction), was modelled the hGαs protein for the first time and validated this model by site-directed mutagenesis. By molecular docking, we identified some potential regions of interaction between hGαs and EtOH that are located on the SIII and SI regions of the Gαs. Therefore, we conclude that ethanol increases the interaction between α1GlyR and Gβγ in HEK293 cells, an effect that might be attributed to the interaction between EtOH and hGαs, which consequently stimulates hGαs.

关键词： Alcoholism Ethanol Glycine Receptor G Proteins Signals Transduction

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Risk Factors and Gender Differences for Depression in Chilean Older Adults:A Cross-Sectional Analysis from the National Health Survey 2016–2017

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International Journal of Mental Health Promotion 2022年第5期24卷 679-697页

作者： Gabriela Nazar Carlos-María Alcover Yeny Concha-Cisternas Igor Cigarroa ximena Díaz-Martínez Mariela Gatica-Saavedra Fabián Lanuza Ana María Leiva-Ordónez María Adela Martínez-Sanguinetti Miquel Martorell Fanny Petermann-Rocha Claudia Troncoso-Pantoja Carlos Celis-Morales Departamento de Psicología Facultad de Ciencias SocialesUniversidad de ConcepciónConcepciónChile Centro de Vida Saludable Universidad de ConcepciónConcepciónChile Departamento de Psicología Facultad de Ciencias de la SaludUniversidad Rey Juan CarlosMadridEspaña Pedagogía en Educación Física Facultad de EducaciónUniversidad AutónomaTalcaChile Escuela de Kinesiología Facultad de SaludUniversidad Santo TomásLosÁngelesChile Grupo de Investigación Calidad de Vida Departamento de Ciencias de la EducaciónUniversidad del BiobíoChillánChile Depto de Psiquiatría y Salud Mental Facultad de MedicinaUniversidad de ConcepciónConcepciónChile Centro de Epidemiología Cardiovascular y Nutricional(EPICYN) Facultad de MedicinaUniversidad de La FronteraTemucoChile Biomarkers and Nutrimetabolomics Laboratory Department of NutritionFood Sciences and GastronomyFood Technology Reference Net(XIA)Nutrition and Food Safety Research Institute(INSA)Faculty of Pharmacy and Food SciencesUniversity of BarcelonaBarcelona08028Spain Instituto de Anatomía Histología y PatologíaFacultad de MedicinaUniversidad Austral de ChileValdiviaChile Instituto de Farmacia Facultad de CienciasUniversidad Austral de ChileValdiviaChile Departamento de Nutrición y Dietética Facultad de Farmacia.Universidad de ConcepciónConcepciónChile Facultad de Medicina Universidad Diego PortalesSantiagoChile BHF Glasgow Cardiovascular Research Centre Institute of Cardiovascular and Medical SciencesUniversity of GlasgowGlasgowUK Centro de Investigación en Educación y Desarrollo(CIEDE-UCSC).Departamento de Salud Pública Facultad de MedicinaUniversidad Católica de la Santísima ConcepciónConcepciónChile Laboratorio de Rendimiento Humano Grupo de Estudio en EducaciónActividad Física y Salud(GEEAFyS)Universidad Católica del MauleTalcaChile

Depressive disorders are recognized as one of the most common mental health conditions across different age ***,the risk factors associated with depression among older people from low-and middle-income countries remains *** study aims to identify socio-demographic,health and psychosocial-related factors associated with depression in Chilean older adults.A cross-sectional study was carried out in a representative sample of 1,765 adults aged≥60 years participants from the Chilean National Health Survey 2016–***-sion was assessed with the Composite International Diagnostic Interview(CIDI-SF).Associations between the exposure variables and depression were investigated using Poisson regression *** mainﬁndings indi-cated that women showed higher likelihood of depression than men(Prevalence Ratio(PR)=2.6[95%CI:1.40;4.89]).An increased likelihood of depression was found in older adults with chronic pain,multimorbidity(≥2 diseases),previous diagnose of depression,high perception of stress,ﬁnancial stress,and difﬁculties for social *** women,higher likelihood of depression was found for those with the frailty phenotype(PR:8.53[95%CI:1.68;43.32]),rheumatoid arthritis(PR:2.41[95%CI:1.34;4.34]),insomnia(PR:2.99[95%CI:1.74;5.12])and low self-rated well-being(PR:4.94[95%CI:2.26;10.79]).Men who were divorced(PR:7.10[95%CI:1.44;34.90])or widowed(PR:10.83[95%CI:3.71;31.58]),obese(PR:5.08[95%CI:1.48;17.42])and who had asthma(PR:7.60[95%CI:2.31;24.99])were associated with higher odds of *** currentﬁndings may have clinical implications for the early identiﬁcation of older adults more susceptible to depression and also suggest the need to implement cultural and age-sensitive strategies to promote mental health in late life.

关键词： Depression older adults aging late-life depression risk factors

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Yeyes and Nainais,Opas and Omas

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Beijing Review 2024年第23期67卷 48-48页

作者： ximena Maite Villegas Cabrolier East China Normal University

0n sunny days,families come to visit the campus of my *** only parents with young children,but also adult children with their senior parents,and even elderly couples take walks along the beautiful Liwa River that runs... 详细信息

关键词： sunny beautiful days

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Yeyes and Nainais,Opas and Omas

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Beijing Review 2024年第23期 48页

作者： ximena Maite Villegas Cabrolier

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Antioxidant activity of Bios-p peptide analogue in HEK293T cells and three-dimensional structure prediction

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Advances in Bioscience and Biotechnology 2013年第1期4卷 55-61页

作者： Pamela Fernández Manzano Ignacio Jofré Fernández Fabiola Zambrano Patricia Navarrete Gómez Magdalena Cuevas Troncoso ximena Romo Marty Andrés ávila Barrera Fernando Romero Mejía Center of Neurosciences and Peptides Biology Universidad de La Frontera Temuco Chile Centro de Modelación y Computación Científica Universidad de La Frontera Temuco Chile Departamento de Ciencias Biologicas Facultad de Ciencias Biologicas Universidad Andres Bello Talcahuano Chile

Studies had indicate that excessive production of reactive oxygen species (ROS) affect cellular signaling pathways, which is associated with pathological and physiological conditions such as cancer, diabetes and neurodegenerative diseases In this context, our laboratory has obtained the Bios-p, a ROS modulator, peptide analogue by sequencing from the seed of Bauhinia bauhinoides, which represents the active 12-amino acid, obtained from the inhibitor BbKI protease and we predicted the three-dimensional structure of Bios-p analogue peptide using homology modeling, being patented by the working group of Dr. Maria Luiza Vilela Oliva of UNIFESP, Brazil (a member of our cluster). The protective effect on the viability and antioxidant capacity of Bios-p was studied in HEK 293T cells under oxidative stress induced by hydrogen peroxide (H2O2) using SYTOXGREEN/DHE and luminescence assay. The three-dimensional structure of Bios-p peptide analogue was predicted by homology-based modeling using Modeller9v8. The pretreatment with different concentrations of Bios-p (1 μM - 10 μM) showed an increase of 53.83% ± 3.86% the cellular viability in under oxidative stress compared to control. Furthermore, the results to indicate that HEK293T cells by incubating for 24 h with Bios-p shown a significant decreased of basal extracellular ROS on total cell population in 89.67% ± 0.76%, compared to control in the absence of the analogue. Similarly it is observed that Bios-p has a significant antioxidant effect on extracellular ROS production when cells are subjected to oxidative stress induced by 200 μM H2O2 in 64.37% ± 4.63%, compared to control in absence of H2O2 and Bios-p. These results suggest that Bios-p has potential as antioxidant agent in cells HEK293T under H2O2-induced oxidative stress and that can protect the cells viability as concentration-dependent, and we propose a new biotechnological tool for modulate the ROS production.

关键词： Antioxidant Bios-p Bauhinia bauhinoides

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Critically ill patients with cancer: A clinical perspective

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World Journal of Clinical Oncology 2020年第10期11卷 809-835页

作者： Frank Daniel Martos-Benítez Caridad de Dios Soler-Morejón Karla ximena Lara-Ponce Versis Orama-Requejo DailéBurgos-Aragüez Hilev Larrondo-Muguercia Rahim W Lespoir Intensive Care Unit 8B Hermanos Ameijeiras HospitalHavana 10300Cuba

Cancer patients account for 15%of all admissions to intensive care unit(ICU)and 5%will experience a critical illness resulting in ICU *** rates have decreased during the last decades because of new anticancer therapies and advanced organ support *** early critical care and organ support is associated with improved survival,timely identification of the onset of clinical signs indicating critical illness is crucial to avoid *** article focused on relevant and current information on epidemiology,diagnosis,and treatment of the main clinical disorders experienced by critically ill cancer patients.

关键词： Acute respiratory failure Cancer Cardiotoxicity Chemotherapy Critical care Infection Mechanical ventilation Neutropenia Postoperative Sepsis

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手术范围对膝关节术后纤维化形成的影响

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中国骨伤 2018年第6期31卷 587-590页

作者：吴海啸 Karen A.Egiazaryan Andrei Р.Ratyev Guram D.Lazishvili ximena A.Sanchez Sanchez 张超俄罗斯联邦国立医科大学莫斯科俄罗斯1179972 俄罗斯圣彼得堡国立大学圣彼得堡俄罗斯199034 天津医科大学肿瘤医院骨与软组织肿瘤科天津300060

膝关节纤维化是术后常见的并发症,为影响手术疗效的关键因素之一,常引起关节活动度降低、慢性关节痛、软骨损伤以及关节废用性病变,严重降低了患者的生活质量。因此,如何降低术后膝关节纤维化的发生是提高手术预期疗效的关键,也是临床... 详细信息

膝关节纤维化是术后常见的并发症,为影响手术疗效的关键因素之一,常引起关节活动度降低、慢性关节痛、软骨损伤以及关节废用性病变,严重降低了患者的生活质量。因此,如何降低术后膝关节纤维化的发生是提高手术预期疗效的关键,也是临床医学上需要亟待解决的重要问题。笔者团队结合自身前期及最新的研究发现,膝关节纤维化的程度与手术范围大小有重要的关联,利用微创和关节镜方法治疗膝关节三室内的疾病可有效减少滑膜、软骨和脂肪垫组织的损伤,同时结合术后早期康复训练是未来预防术后膝关节纤维化的有效手段。

关键词：膝关节纤维化血管内皮生长因子外科手术

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Seroprevalence study of infection with influenza A(H1N1)pdm09 virus in San Felipe Town, Chile

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World Journal of Cardiovascular Diseases 2013年第7期3卷 476-482页

作者： Olea Andrea Fasce Rodrigo Aguilera ximena Oliva Otavio Munoz Sergio Garcia Maritza Pedroni Elena Najera Manuel Guerrero Andrea Bustos Patricia Mora Judith Vega Jeanette Cerda Jaime Abarca Katia McCarron Meg Widdowson Marc-Alain Castillo Ernesto Alvarado Sergio Quijada Sandra Gallegos Doris Gonzalez Claudia Center of Epidemiology and Health Policies Department Faculty of MedicineUniversidad del DesarrolloConcepcionChile Section of Respiratory and Exanthematous viruses Sub-Department of Clinical VirologyInstitute of Public HealthMinistry of HealthSantiagoChile Center of Epidemiology and Health Policies of the Universidad del Desarrollo ConcepcionChile Panamerican Health Organization Washington DCUSA Public Health Department and Pediatrics Division Faculty of MedicinePontifical Catholic University of ChileSantiagoChile Centers for Diseases Control and Prevention AtlantaUSA Center for Micro Data Economics DepartmentUniversity of ChileSantiagoChile Epidemiology and Health Policies Department Universidad del DesarrolloConcepcionChile

Objective: To know the natural history of the first wave of pandemic influenza A(H1N1)pdm09 in the Southern hemisphere, through the detection of antibodies against influenza A(H1N1)pdm09 in a selected community, to estimate the population attack rate and its variations, the consultation rates, hospitallization and mortality rates. Methodology: A representative random sample of the population of a commune in Chile (San Felipe) was interviewed and taken blood samples between January and March 2010. A study against the antibodies of the influenza A(H1N1) pdm09 virus was conducted, by the technique of the Hemaglutination Inhibition (HAI) according to standardized methodology. Subjects with antibody titers ≥1:40 were considered positive. Results: 13.5% of the population of San Felipe had antibodies against influenza A(H1N1)pdm09;this percentage reached 30% of the population between 0 and 18 years and 6.1% among those over 19 years. The age variable was the only factor that evidenced significant differences in the prevalence of antibodies. There were no significant differences related to gender, vaccination history against seasonal inluenza, or comorbidity. 51% of people with positive serology showed IN-FLUENZA-LIKE SYMPTOMS. Conclusions: A relevant percentage of subclinical disease was detected in the first pandemic wave in Chile and the proportion of people with SARI and deaths was small. Data from epidemiological surveillance were useful to estimate the trend of TSI but not its magnitude.

关键词： Influenza A(H1N1)pdm09 Influenza Seroprevalence

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Soil organic carbon stocks in native forest of Argentina:a useful surrogate for mitigation and conservation planning under climate variability

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Ecological Processes 2024年第1期13卷 3-24页

作者： Pablo L.Peri Juan Gaitán Matías Mastrangelo Marcelo Nosetto Pablo E.Villagra Ezequiel Balducci Martín Pinazo Roxana P.Eclesia Alejandra Von Wallis Sebastián Villarino Francisco Alaggia Marina González Polo Silvina Manrique Pablo A.Meglioli Julián Rodríguez-Souilla Martín Mónaco Jimena E.Chaves Ariel Medina Ignacio Gasparri Eugenio Alvarez Arnesi María Paula Barral Axel von Müller Norberto M.Pahr Josefina Uribe Echevarria Pedro Fernández Marina Morsucci Dardo López Juan Manuel Cellini Leandro Alvarez Ignacio Barberis Hernán Colomb Ludmila La Manna Sebastián Barbaro Cecilia Blundo ximena Sirimarco Laura Cavallero Gualberto Zalazar Guillermo Martínez Pastur Instituto Nacional de Tecnología Agropecuaria (INTA) Universidad Nacional de la Patagonia Austral (UNPA) Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET ) Universidad Nacional de Luján Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET ) Grupo de Estudio de Agroecosistemas y Paisajes Rurales (GEAP) Universidad Nacional de Mar del Plata Consejo Nacional de Investigaciones Científicas y Técnicas(CONICET ) CCT San Luis Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET ) Instituto Argentino de Nivología Glaciología y Ciencias Ambientales (IANIGLA) Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET ) Universidad Nacional de Cuyo Instituto de Investigaciones en Biodiversidad y Medioambiente (INIBIOMA) Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET ) Instituto de Investigaciones en Energía No Convencional CCT Salta-JujuyConsejo Nacional de Investigaciones Científicas y Técnicas (CONICET ) Laboratorio de Recursos Agroforestales Centro Austral de Investigaciones Científicas (CADIC) Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET ) Dirección Nacional de Bosques Ministerio de Ambiente y Desarrollo Sostenible de la Nación Instituto de Ecología Regional Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET )Universidad Nacional de Tucumán Universidad Nacional de Rosario Instituto de Investigaciones en Ciencias Agrarias de Rosario Centro Científico Tecnológico CONICET Grupo de Estudio de Agroecosistemas y Paisajes Rurales (GEAP) Universidad Nacional de Mar del Plata Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET ) Laboratorio de Investigaciones en Maderas (LIMAD) Universidad Nacional de La Plata (UNLP) Administración de Parques Nacionales (APN) Parque Nacional Los Alerces Centro de Estudios Ambientales Integrados (CEAI) Universidad Nacional de la Patagonia San Juan Bosco (UNPASJB) Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET )

Background The nationally determined contribution（NDC） presented by Argentina within the framework of the Paris Agreement is aligned with the decisions made in the context of the United Nations Framework Conven-tion on Climate Change（UNFCCC） on the reduction of emissions derived from deforestation and forest degradation, as well as forest carbon conservation（REDD+）. In addition, climate change constitutes one of the greatest threats to forest biodiversity and ecosystem services. However, the soil organic carbon（SOC） stocks of native forests have not been incorporated into the Forest Reference Emission Levels calculations and for conservation planning under cli-mate variability due to a lack of information. The objectives of this study were:（i） to model SOC stocks to 30 cm of native forests at a national scale using climatic, topographic and vegetation as predictor variables, and（ii） to relate SOC stocks with spatial–temporal remotely sensed indices to determine biodiversity conservation concerns due to threats from high inter-annual climate *** We used 1040 forest soil samples（0–30 cm） to generate spatially explicit estimates of SOC native forests in Argentina at a spatial resolution of approximately 200 m. We selected 52 potential predictive environmental covari-ates, which represent key factors for the spatial distribution of SOC. All covariate maps were uploaded to the Google Earth Engine cloud-based computing platform for subsequent modelling. To determine the biodiversity threats from high inter-annual climate variability, we employed the spatial–temporal satellite-derived indices based on Enhanced Vegetation Index（EVI） and land surface temperature（LST） images from Landsat *** SOC model（0–30 cm depth） prediction accounted for 69% of the variation of this soil property across the whole native forest coverage in Argentina. Total mean SOC stock reached 2.81 Pg C（2.71–2.84 Pg C with a probability of 90%） for a total area of 460,790 km2,

关键词： Soil organic carbon Biodiversity Native forest Climate change mitigation Modelling Resilience

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接受他汀治疗患者新发糖尿病风险：人群为基础的研究

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英国医学杂志中文版 2013年第5期16卷 268-269页

作者： Aleesa A Caner Tara Gomes ximena Camacho David N Juurlink Baiju R Shah Muhammad M Mamdani 张秀珍(译) 骆斯慧(译) 翁建平(校)

目的研究接受HMG-CoA还原酶抑制剂（他汀类药物）治疗的患者的新发糖尿病风险。设计人群为基础的队列研究，以时间事件分析的方法评估各种他汀类药物应用和新发糖尿病风险的相关性分析。计算危害比（hazard ratios，HR）来确定他汀的... 详细信息

目的研究接受HMG-CoA还原酶抑制剂（他汀类药物）治疗的患者的新发糖尿病风险。设计人群为基础的队列研究，以时间事件分析的方法评估各种他汀类药物应用和新发糖尿病风险的相关性分析。计算危害比（hazard ratios，HR）来确定他汀的种类及剂量与新发糖尿病的关系。

关键词：他汀类药物糖尿病 HMG-CoA还原酶抑制剂风险人群患者治疗相关性分析

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