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Landscape of human organoids:Ideal model in clinics and research

The Innovation 2024年第3期5卷 146-164页

作者： Xinxin Han Chunhui Cai Wei Deng Yanghua Shi Lanyang Li Chen wang Jian Zhang Mingjie Rong Jiping Liu Bangjiang Fang Hua He xiling Liu Chuxia Deng Xiao He Xin Cao Organ Regeneration X Lab Lisheng East China Institute of BiotechnologyPeking UniversityJiangsu 226200China Shanghai Lisheng Biotech Shanghai 200092China LongHua Hospital Shanghai University of Traditional Chinese Medicine725 Wanping South RoadXuhui DistrictShanghai 200032China Department of Oncology Shanghai Ninth People’s HospitalShanghai Jiao Tong University School of MedicineShanghai 200125China Department of Neurosurgery Third Affiliated HospitalNaval Medical UniversityShanghai 200438China Shanghai Key Laboratory of Forensic Medicine Shanghai Forensic Service PlatformAcademy of Forensic ScienceMinistry of JusticeShanghai 200063China Cancer Center Faculty of Health SciencesUniversity of MacaoTaipaMacao SARChina Ministry of Education Frontiers Science Center for Precision Oncology University of MacaoTaipaMacao SAR 999078China CAS Key Lab for Biomedical Effects of Nanomaterials and Nanosafety Institute of High Energy PhysicsChinese Academy of SciencesBeijing 100049China Zhongshan Hospital Institute of Clinical Science Fudan University Shanghai Medical CollegeShanghai 200032China

In the last decade,organoid research has entered a golden era,signifying a pivotal shift in the biomedical landscape.The year 2023 marked a milestone with the publication of thousands of papers in this arena,reflecting exponential growth.However,amid this burgeoning expansion,a comprehensive and accurate overview of the field has been conspicuously absent.Our review is intended to bridge this gap,providing a panoramic view of the rapidly evolving organoid landscape.We meticulously analyze the organoid field from eight distinctive vantage points,harnessing our rich experience in academic research,industrial application,and clinical practice.We present a deep exploration of the advances in organoid technology,underpinned by our long-standing involvement in this arena.Our narrative traverses the historical genesis of organoids and their transformative impact across various biomedical sectors,including oncology,toxicology,and drug development.We delve into the synergy between organoids and avant-garde technologies such as synthetic biology and single-cell omics and discuss their pivotal role in tailoring personalized medicine,enhancing high-throughput drug screening,and constructing physiologically pertinent disease models.Our comprehensive analysis and reflective discourse provide a deep dive into the existing landscape and emerging trends in organoid technology.We spotlight technological innovations,methodological evolution,and the broadening spectrum of applications,emphasizing the revolutionary influence of organoids in personalized medicine,oncology,drug discovery,and other fields.Looking ahead,we cautiously anticipate future developments in the field of organoid research,especially its potential implications for personalized patient care,new avenues of drug discovery,and clinical research.We trust that our comprehensive review will be an asset for researchers,clinicians,and patients with keen interest in personalized medical strategies.We offer a broad view of the present and prospective capabilities of organoi

关键词： landscape intended advances

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Molecular basis of heading date control in rice

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aBIOTECH 2020年第4期1卷 219-232页

作者： Hua Wei xiling wang Hang Xu Lei wang Key Laboratory of Plant Molecular Physiology CAS Center for Excellence in Molecular Plant SciencesInstitute of BotanyChinese Academy of SciencesBeijing 100093China University of Chinese Academy of Sciences Beijing 100049China

Flowering time is of great significance for crop reproduction,yield,and regional adaptability,which is intricately regulated by various environmental cues and endogenous signals.Genetic approaches in Arabidopsis have revealed the elaborate underlying mechanisms of sensing the dynamic change of photoperiod via a coincidence between light signaling and circadian clock,the cellular time keeping system,to precisely control photoperiodic flowering time,and many other signaling pathways including internal hormones and external temperature cues.Extensive studies in rice(Oryza sativa.),one of the short-day plants(SDP),have uncovered the multiple major genetic components in regulating heading date,and revealed the underlying mechanisms for regulating heading date.Here we summarize the current progresses on the molecular basis for rice heading date control,especially focusing on the integration mechanism between photoperiod and circadian clock,and epigenetic regulation and heading procedures in response to abiotic stresses.

关键词： Heading date Rice Photoperiod Circadian clock Epigenetic modification Abiotic stress

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Chromosome-level Genomes Reveal the Genetic Basis of Descending Dysploidy and Sex Determination in Morus Plants

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Genomics, Proteomics & Bioinformatics 2022年第6期20卷 1119-1137页

作者： Zhongqiang Xia Xuelei Dai Wei Fan Changying Liu Meirong Zhang Peipei Bian Yuping Zhou Liang Li Baozhong Zhu Shuman Liu Zhengang Li xiling wang Maode Yu Zhonghuai Xiang Yu Jiang Aichun Zhao State Key Laboratory of Silkworm Genome Biology Institute of Sericulture and Systems BiologySouthwest UniversityChongqing 400716China Key Laboratory of Animal Genetics Breeding and Reproduction of Shaanxi ProvinceCollege of Animal Science and TechnologyNorthwest A&F UniversityYangling 712100China Key Laboratory of Coarse Cereal Processing Ministry of Agriculture and Rural AffairsChengdu UniversityChengdu 610106China The Sericultural and Apicultural Research Institute Yunnan Academy of Agricultural SciencesMengzi 661100China College of Sericulture Textile and Biomass SciencesSouthwest UniversityChongqing 400716China

Multiple plant lineages have independently evolved sex chromosomes and variable karyotypes to maintain their sessile lifestyles through constant biological innovation.Morus notabilis,a dioecious mulberry species,has the fewest chromosomes among Morus spp.,but the genetic basis of sex determination and karyotype evolution in this species has not been identified.In this study,three high-quality genome assemblies were generated for Morus spp.[including dioecious M.notabilis(male and female)and Morus yunnanensis(female)]with genome sizes of 301–329 Mb and were grouped into six pseudochromosomes.Using a combination of genomic approaches,we found that the putative ancestral karyotype of Morus species was close to 14 protochromosomes,and that several chromosome fusion events resulted in descending dysploidy(2n=2x=12).We also characterized a~6.2-Mb sex-determining region on chromosome 3.Four potential male-specific genes,a partially duplicated DNA helicase gene(named MSDH)and three Ty3_Gypsy long terminal repeat retrotransposons(named MSTG1/2/3),were identified in the Y-linked area and considered to be strong candidate genes for sex determination or differentiation.Population genomic analysis showed that Guangdong accessions in China were genetically similar to Japanese accessions of mulberry.In addition,genomic areas containing selective sweeps that distinguish domesticated mulberry from wild populations in terms of flowering and disease resistance were identified.Our study provides an important genetic resource for sex identification research and molecular breeding in mulberry.

关键词： Mulberry Karyotype evolution Dioecy Sex determination Population genomics

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坚持还是放弃:失败恐惧的认知效应及其对创业坚持的影响

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南京审计大学学报 2024年第4期21卷 34-42页

作者：郝喜玲苟亚萍王敬涵安徽财经大学工商管理学院安徽蚌埠233000

基于情绪认知评价理论,从情绪和认知两个视角分析失败恐惧对创业坚持的影响与边界条件。通过对316名创业者的问卷调查发现:创业失败恐惧对创业坚持行为具有负向影响;自我效能感在失败恐惧和创业坚持之间发挥中介作用;成长型思维调节失... 详细信息

基于情绪认知评价理论,从情绪和认知两个视角分析失败恐惧对创业坚持的影响与边界条件。通过对316名创业者的问卷调查发现:创业失败恐惧对创业坚持行为具有负向影响;自我效能感在失败恐惧和创业坚持之间发挥中介作用;成长型思维调节失败恐惧对自我效能感的负向影响,成长型思维通过降低失败恐惧对自我效能感的负向作用来促进创业坚持行为。依据“情感—认知—行为”的逻辑从创业者的情绪和认知视角综合分析失败恐惧对创业坚持的影响,既为创业者中途退出创业活动提供解释思路和理论洞见,也为全面理解创业坚持行为提供理论指导。

关键词：失败恐惧创业坚持自我效能感成长型思维情绪认知评价理论创业行为创业认知

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Effect of Panaxtriol Saponins on synaptophysin and postsynaptic density-95 expression at different periods of cerebral infarction

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Neural Regeneration Research 2008年第11期3卷 1212-1217页

作者： Fangyuan Cui Jiangying Zhai Weimeng Zou xiling wang Yihuai Zou Linggqun Zhu Department of Neurology Dongzhimen Hospital Affiliated to Beijing University of Traditional Chinese Medicine Beijing 100700 China Key Laboratory of Chinese Internal Medicine Dongzhimen Hospital Affiliated to Beijing University of Traditional Chinese Medicine Beijing 100700 China

BACKGROUND： The change in expression of synaptophysin （Syp） and postsynaptic density-95 （PSD-95） alters after cerebral infarction, and the plasticity of synapses contributes greatly to nerve function recovery. Chinese medicinal substances may play an important role in the expression of Syp and PSD-95. OBJECTIVE： To observe the effect of Panaxtriol Saponins （PTS）, an active component in Sanqi tongshu capsules, on the expression of Syp and PSD-95 after cerebral infarction at different time points in rats, so as to examine the cerebral function remodeling mechanism. DESIGN, TIME AND SETTING： A randomized and controlled observation which was performed in Dongzhimen Hospital, Beijing University of Traditional Chinese Medicine from January to March, 2007. MATERIALS： Twenty-six healthy male Sprague Dawley rats were used to establish middle cerebral artery occlusion based on the Longa method. Sanqi tongshu capsules （containing 100 mg PTS per tablet） were provided by the Chengdu Huashen Group and nimodipine tablets （30 mg） by Tianjin Zhongyang Pharmaceutical Co., Ltd. METHODS： Twenty-six rats were randomly divided into an operation group （n = 21 ） and a control group （n = 5）. The operation group underwent the EZ Longa procedure to make the middle cerebral artery occlusion model. After surgery rats were randomly divided into a model group, a PTS group and a nimodipine group, with seven rats in each group. Rats were intragastrically administrated with saline （2 mL/d） in the model group, with Sanqi tongshu capsule （5.4 mg/100 g/d） in the PTS group, and with nimodipine （1.73 mg/100 g/d） in the nimodipine group. Rats in the control group did not undergo model establishment and drug administration. MAIN OUTCOME MEASURES： The expressions of Syp and PSD-95 were measured by immunohistochemical and image analysis at days 3, 7 and 28 after the operation. RESULTS： The expression of Syp and PSD-95 in the operation group was significantly lower than in the control group at days 3, 7, 28 postoperatively （P 〈 0.05）. The expression of Syp

关键词： Panaxtriol Saponins cerebral infarction remodeling synaptophysin postsynaptic density-95

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基于压电驱动的圆柱壳内振源振动传递控制

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振动与冲击 2024年第1期43卷 246-251页

作者：贺佩韬王双立耿小明谢溪凌张志谊上海交通大学机械系统与振动国家重点实验室上海200240 上海交通大学振动、冲击与噪声研究所上海200240 中国船舶集团有限公司第705研究所西安710077

针对圆柱壳内振源振动通过环形支承向壳体传递的问题,提出一种基于压电作动器的主被动支承隔振方案,并通过振源-主被动支承-壳体耦合系统进行振动控制分析。根据Flugge壳理论,采用波传播法建立圆柱壳体振动模型,同时采用有限元方法建立... 详细信息

针对圆柱壳内振源振动通过环形支承向壳体传递的问题,提出一种基于压电作动器的主被动支承隔振方案,并通过振源-主被动支承-壳体耦合系统进行振动控制分析。根据Flugge壳理论,采用波传播法建立圆柱壳体振动模型,同时采用有限元方法建立振源及主被动支承的振动模型,最终使用子结构频响函数综合获得耦合系统振动模型。基于耦合系统模型和理想控制假设,在频域给出壳体振动可控性,并通过振源-主被动支承-壳体试验系统验证控制的有效性。仿真与试验结果表明,基于压电作动器的主被动隔振方案能显著降低壳体的宽带和线谱振动。

关键词：主动控制压电驱动振动传递圆柱壳体

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Agrobacterium–mediated Transformation of Kalanchoe laxiflora

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Horticultural Plant Journal 2019年第5期5卷 221-228页

作者： xiling wang Xinlu Chen Qunkang Cheng Kaikai Zhu Xiaohan Yang Zongming Cheng Department of Plant Sciences University of TennesseeKnoxvilleTN37996USA College of Biotechnology Southwest UniversityChongqing400715China Department of Entomology and Plant Pathology University of TennesseeKnoxvilleTN37996USA College of Horticulture Nanjing Agricultural UniversityNanjing210095China Biosciences Division Oak Ridge National LaboratoryOak RidgeTN37831-6422USA

Plants with crassulacean acid metabolism(CAM) generally utilize water 20%–80% more efficiently than non-CAM plants. The whole genomes of several CAM plants have been sequenced or are being sequenced. For effective genome characterization and genome editing of CAM plants,an efficient transformation system is essential. In this study, we developed an Agrobacterium-mediated transformation protocol for Kalanchoe laxiflora, an obligate CAM plant,by optimizing several factors affecting the transformation efficiency. Agrobacterium strains AGL1, C58, EHA105,and GV3101 were all suitable for K. laxiflora transformation. Fifty-nine percent of the leaf explants yielded kanamycin-resistant and GUS-positive shoots. Polymerase chain reaction and quantitative real-time PCR(qRT-PCR) using gus A-, gus Plus-, npt II-and hpt-specific primers confirmed that the transgenes were integrated into K. laxiflora genome and expressed. This efficient transformation system will allow effective functional characterization of genes through over-or down-expression, knockout, or genome editing.

关键词： Kalanchoe laxiflora Agrobacterium-mediated transformation CAM plants

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舱段主动隔振系统作动器配置优化

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振动与冲击 2024年第1期43卷 91-98页

作者：巫頔谢溪凌张志谊上海交通大学机械系统与振动国家重点实验室上海200240 上海交通大学振动、冲击、噪声研究所上海200240

针对舱段主动隔振系统中作动器配置优化问题,给出一种优化模型和方法,通过数值计算进行方法验证。首先建立了多通道舱段主动隔振系统的动力学模型,然后将作动器配置优化转换为约束0-1非线性规划问题,以系统监测点响应为优化目标函数,作... 详细信息

针对舱段主动隔振系统中作动器配置优化问题,给出一种优化模型和方法,通过数值计算进行方法验证。首先建立了多通道舱段主动隔振系统的动力学模型,然后将作动器配置优化转换为约束0-1非线性规划问题,以系统监测点响应为优化目标函数,作动器启用状态为自变量,最后采用教与学优化(teaching and learning-based optimization,TLBO)算法寻找最优配置。仿真计算结果表明,对于不同的激励,多通道主动隔振系统的最优配置不同,即存在对应给定激励下抑制壳体振动与声辐射的最优配置。

关键词：主动振动控制教与学算法(TLBO) 配置优化

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芥末精油微胶囊对草莓保鲜品质的研究

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农产品加工 2024年第6期 20-22,26页

作者：杜喜玲鹤壁职业技术学院食品工程学院河南鹤壁458030 鹤壁市绿色食品深加工重点实验室河南鹤壁458030

探究芥末精油-β-环糊精微胶囊可以作为天然的保鲜剂,延缓草莓的呼吸作用和抑制微生物生长繁殖,延长果实的贮藏期,采用壁材∶芯材(6∶1)配比制作芥末精油-β-环糊精微胶囊,草莓分别在微胶囊与对照组下考查了草莓的失重率、硬度、可溶性... 详细信息

探究芥末精油-β-环糊精微胶囊可以作为天然的保鲜剂,延缓草莓的呼吸作用和抑制微生物生长繁殖,延长果实的贮藏期,采用壁材∶芯材(6∶1)配比制作芥末精油-β-环糊精微胶囊,草莓分别在微胶囊与对照组下考查了草莓的失重率、硬度、可溶性固形物含量、维C含量等保鲜品质参数。结果表明,对照组的草莓在室温下贮存7 d后的失重率比芥末精油微胶囊组高,而硬度、可溶性固形物含量、维C含量则比芥末精油微胶囊组低。由此可见,芥末精油微胶囊处理后的草莓在一定程度上可以延长保质期,提高草莓的保鲜作用,为开发芥末天然保鲜剂提供一定的理论基础。

关键词：草莓芥末精油 β-环糊精微胶囊保鲜品质

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心电及血压指标在高血压合并左心室肥厚中的应用进展

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实用心电学杂志 2024年第3期33卷 312-315页

作者：张希铃王新康福建医科大学省立临床医学院福建福州350001 福建省立医院心电诊断科福建福州350008

高血压是常见的心血管疾病,左心室肥厚(left ventricular hypertrophy,LVH)是其常见的并发症。心电及血压指标是临床上用于评估心血管疾病的常见指标。近年来,随着检测技术的进步,它们在评价LVH方面展现出重要的应用价值。本文综述心电... 详细信息

高血压是常见的心血管疾病,左心室肥厚(left ventricular hypertrophy,LVH)是其常见的并发症。心电及血压指标是临床上用于评估心血管疾病的常见指标。近年来,随着检测技术的进步,它们在评价LVH方面展现出重要的应用价值。本文综述心电及血压指标在LVH中的临床应用进展,并对未来的研究趋势进行展望。

关键词：高血压左心室肥厚心电图动态心电图动态血压监测

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