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Agrobacterium–mediated Transformation of Kalanchoe laxiflora

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Horticultural Plant Journal 2019年第5期5卷 221-228页

作者： xiling wang Xinlu Chen Qunkang Cheng Kaikai Zhu Xiaohan Yang Zongming Cheng Department of Plant Sciences University of TennesseeKnoxvilleTN37996USA College of Biotechnology Southwest UniversityChongqing400715China Department of Entomology and Plant Pathology University of TennesseeKnoxvilleTN37996USA College of Horticulture Nanjing Agricultural UniversityNanjing210095China Biosciences Division Oak Ridge National LaboratoryOak RidgeTN37831-6422USA

Plants with crassulacean acid metabolism(CAM) generally utilize water 20%–80% more efficiently than non-CAM plants. The whole genomes of several CAM plants have been sequenced or are being sequenced. For effective genome characterization and genome editing of CAM plants,an efficient transformation system is essential. In this study, we developed an Agrobacterium-mediated transformation protocol for Kalanchoe laxiflora, an obligate CAM plant,by optimizing several factors affecting the transformation efficiency. Agrobacterium strains AGL1, C58, EHA105,and GV3101 were all suitable for K. laxiflora transformation. Fifty-nine percent of the leaf explants yielded kanamycin-resistant and GUS-positive shoots. Polymerase chain reaction and quantitative real-time PCR(qRT-PCR) using gus A-, gus Plus-, npt II-and hpt-specific primers confirmed that the transgenes were integrated into K. laxiflora genome and expressed. This efficient transformation system will allow effective functional characterization of genes through over-or down-expression, knockout, or genome editing.

关键词： Kalanchoe laxiflora Agrobacterium-mediated transformation CAM plants

Flow imaging method of electromagnetic measurement in well logging

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Science China Earth Sciences 2008年第S2期51卷 181-185页

作者： WU xiling,wang XiaoXing,ZHAO YanWei & YANG Mei School of Resource and Information Technology,China University of Petroleum,Beijing 102249,China China Univ Petr Sch Resource & Informat Technol Beijing 102249 Peoples R China

Multiphase flow in an oil well is a dynamic phenomenon of inhomogeneous medium,which should be studied in a nonlinear way. This paper presents an electromagnetic measurement method based on the electrical property differences between oil,gas and water to scan,inverse and show the distribution and condition of multiphase flow. Both numerical simulation and physical experiments have proved that clear images could be obtained by this way.

关键词： well multiphase flow electromagnetic wave flow imaging

Effect of Panaxtriol Saponins on synaptophysin and postsynaptic density-95 expression at different periods of cerebral infarction

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Neural Regeneration Research 2008年第11期3卷 1212-1217页

作者： Fangyuan Cui Jiangying Zhai Weimeng Zou xiling wang Yihuai Zou Linggqun Zhu Department of Neurology Dongzhimen Hospital Affiliated to Beijing University of Traditional Chinese Medicine Beijing 100700 China Key Laboratory of Chinese Internal Medicine Dongzhimen Hospital Affiliated to Beijing University of Traditional Chinese Medicine Beijing 100700 China

BACKGROUND： The change in expression of synaptophysin （Syp） and postsynaptic density-95 （PSD-95） alters after cerebral infarction, and the plasticity of synapses contributes greatly to nerve function recovery. Chinese medicinal substances may play an important role in the expression of Syp and PSD-95. OBJECTIVE： To observe the effect of Panaxtriol Saponins （PTS）, an active component in Sanqi tongshu capsules, on the expression of Syp and PSD-95 after cerebral infarction at different time points in rats, so as to examine the cerebral function remodeling mechanism. DESIGN, TIME AND SETTING： A randomized and controlled observation which was performed in Dongzhimen Hospital, Beijing University of Traditional Chinese Medicine from January to March, 2007. MATERIALS： Twenty-six healthy male Sprague Dawley rats were used to establish middle cerebral artery occlusion based on the Longa method. Sanqi tongshu capsules （containing 100 mg PTS per tablet） were provided by the Chengdu Huashen Group and nimodipine tablets （30 mg） by Tianjin Zhongyang Pharmaceutical Co., Ltd. METHODS： Twenty-six rats were randomly divided into an operation group （n = 21 ） and a control group （n = 5）. The operation group underwent the EZ Longa procedure to make the middle cerebral artery occlusion model. After surgery rats were randomly divided into a model group, a PTS group and a nimodipine group, with seven rats in each group. Rats were intragastrically administrated with saline （2 mL/d） in the model group, with Sanqi tongshu capsule （5.4 mg/100 g/d） in the PTS group, and with nimodipine （1.73 mg/100 g/d） in the nimodipine group. Rats in the control group did not undergo model establishment and drug administration. MAIN OUTCOME MEASURES： The expressions of Syp and PSD-95 were measured by immunohistochemical and image analysis at days 3, 7 and 28 after the operation. RESULTS： The expression of Syp and PSD-95 in the operation group was significantly lower than in the control group at days 3, 7, 28 p

关键词： Panaxtriol Saponins cerebral infarction remodeling synaptophysin postsynaptic density-95

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议我国收视率调查行业规范和管理

中国广播电视学刊 2007年第6期 75-76页

作者：王锡苓中国传媒大学

随着电视日益成为人们日常生活中不可缺少的获取信息、娱乐、进行商业交流的主要媒体时，当广告运营商将目光聚焦在高收视率的电视节目时，电视收视率调查就成为电视台．企业和广告商衡量电视收视市场的晴雨表，而有效、可靠的电视受众... 详细信息

随着电视日益成为人们日常生活中不可缺少的获取信息、娱乐、进行商业交流的主要媒体时，当广告运营商将目光聚焦在高收视率的电视节目时，电视收视率调查就成为电视台．企业和广告商衡量电视收视市场的晴雨表，而有效、可靠的电视受众调查数据便是必不可少的衡量手段。随着中国收视率调查行业的发展，对这一新兴行业的行业管理和规范，至今仍然没有纳入到相应机构的管理范畴之内，视听率调查机构多依靠行业自律或参照国际上已有的行业操作标准进行视听率的调查和管理。因此，有必要了解和认识国际上比较成熟的管理办法和具体操作，为我国视听率调查行业尽快由自律到统一管理奠定一定的基础。

关键词：收视率调查行业管理行业规范电视节目调查行业行业自律调查机构视听率

A comparison study of proxies for influenza activity from different data sources in Shanghai,China

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A comparison study of proxies for influenza activity from di...

Joys and Worries in Chinese Ammonium Chloride Market

2017年中国卫生统计学学术年会

作者： Juanjuan Zhang xiling wang Henry Lynn Department of Biostatistics School of Public HealthFudan University

＜正＞Background:Influenza is associated with considerable morbidity and mortality but its seasonal patterns and effects are complex and not fully understood,complicating disease burden *** study examines the similarities and differences of six proxies for influenza activity in Shanghai,China,and provide practical knowledge for improving the surveillance of influenza.

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China Chemical Reporter 2008年第11期19卷 14-15页

作者： wang xiling,Secretary-General of China Soda Ash Industry Association

1 Overall rehabilitation of the ammonium chloride market China manufactured 5.85 million tons of ammonium chloride from January to Oc-tober *** for 2007 is forecast to exceed 7.00 million tons,an

关键词： fertilizers exceed ammonium manufactured rehabilit

跨越逻辑、方法与范式:计算扎根理论作为创新性研究方法的特征、意义与应用

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新闻界 2023年第6期 83-96页

作者：金鑫王锡苓重庆师范大学新闻与传媒学院重庆401331 中国传媒大学新闻学院北京100024

计算扎根理论是基于社会科学研究第四范式——计算社会科学,所提出的一种创新性研究方法。它以建构主义、现实主义为视角,打破了原本围绕制度化范式对解释主义和实证主义进行严格区分所构建的学术体系,为更加严谨、科学地应对当今传播... 详细信息

计算扎根理论是基于社会科学研究第四范式——计算社会科学,所提出的一种创新性研究方法。它以建构主义、现实主义为视角,打破了原本围绕制度化范式对解释主义和实证主义进行严格区分所构建的学术体系,为更加严谨、科学地应对当今传播学日益凸显的新问题提供了科学路径。基于此,本研究试对计算扎根理论的起源、主旨、特征意义和实践操作进行探究,为其作为创新性研究方法立论,为其促进传统传播学研究转型发展立基。

关键词：传播学范式计算扎根理论

Chromosome-level Genomes Reveal the Genetic Basis of Descending Dysploidy and Sex Determination in Morus Plants

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Genomics, Proteomics & Bioinformatics 2022年第6期20卷 1119-1137页

作者： Zhongqiang Xia Xuelei Dai Wei Fan Changying Liu Meirong Zhang Peipei Bian Yuping Zhou Liang Li Baozhong Zhu Shuman Liu Zhengang Li xiling wang Maode Yu Zhonghuai Xiang Yu Jiang Aichun Zhao State Key Laboratory of Silkworm Genome Biology Institute of Sericulture and Systems BiologySouthwest UniversityChongqing 400716China Key Laboratory of Animal Genetics Breeding and Reproduction of Shaanxi ProvinceCollege of Animal Science and TechnologyNorthwest A&F UniversityYangling 712100China Key Laboratory of Coarse Cereal Processing Ministry of Agriculture and Rural AffairsChengdu UniversityChengdu 610106China The Sericultural and Apicultural Research Institute Yunnan Academy of Agricultural SciencesMengzi 661100China College of Sericulture Textile and Biomass SciencesSouthwest UniversityChongqing 400716China

Multiple plant lineages have independently evolved sex chromosomes and variable karyotypes to maintain their sessile lifestyles through constant biological *** notabilis,a dioecious mulberry species,has the fewest chromosomes among Morus spp.,but the genetic basis of sex determination and karyotype evolution in this species has not been *** this study,three high-quality genome assemblies were generated for Morus spp.[including dioecious ***(male and female)and Morus yunnanensis(female)]with genome sizes of 301–329 Mb and were grouped into six *** a combination of genomic approaches,we found that the putative ancestral karyotype of Morus species was close to 14 protochromosomes,and that several chromosome fusion events resulted in descending dysploidy(2n=2x=12).We also characterized a~6.2-Mb sex-determining region on chromosome *** potential male-specific genes,a partially duplicated DNA helicase gene(named MSDH)and three Ty3_Gypsy long terminal repeat retrotransposons(named MSTG1/2/3),were identified in the Y-linked area and considered to be strong candidate genes for sex determination or *** genomic analysis showed that Guangdong accessions in China were genetically similar to Japanese accessions of *** addition,genomic areas containing selective sweeps that distinguish domesticated mulberry from wild populations in terms of flowering and disease resistance were *** study provides an important genetic resource for sex identification research and molecular breeding in mulberry.

关键词： Mulberry Karyotype evolution Dioecy Sex determination Population genomics

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编码技术改进大规模分布式机器学习性能综述

计算机研究与发展 2020年第3期57卷 542-561页

作者：王艳李念爽王希龄钟凤艳华东交通大学软件学院

由于分布式计算系统能为大数据分析提供大规模的计算能力,近年来受到了人们的广泛关注.在分布式计算系统中,存在某些计算节点由于各种因素的影响,计算速度会以某种随机的方式变慢,从而使运行在集群上的机器学习算法执行时间增加,这种节... 详细信息

由于分布式计算系统能为大数据分析提供大规模的计算能力,近年来受到了人们的广泛关注.在分布式计算系统中,存在某些计算节点由于各种因素的影响,计算速度会以某种随机的方式变慢,从而使运行在集群上的机器学习算法执行时间增加,这种节点叫作掉队节点(straggler).介绍了基于编码技术解决这些问题和改进大规模机器学习集群性能的研究进展.首先介绍编码技术和大规模机器学习集群的相关背景;其次将相关研究按照应用场景分成了应用于矩阵乘法、梯度计算、数据洗牌和一些其他应用,并分别进行了介绍分析;最后总结讨论了相关编码技术存在的困难并对未来的研究趋势进行了展望.

关键词：编码技术机器学习分布式计算掉队节点容忍性能优化