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Immunogenicity of an influenza virus-vectored vaccine carrying the hepatitis C virus protein epitopes in mice

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Immunogenicity of an influenza virus-vectored vaccine carryi...

第十二届全国免疫学学术大会

作者： Yang penghui sun Fang Duan Yueqiang Gu Hongjing Zhang Peirui zhang Shaogeng Wang xiliang Beijing 302 Hospital State Key Laboratory of Pathogens and Biosecurity Beijing Institute of Microbiology and Epidemiology

Hepatitis C virus(HCV) has a devastating impact on human health,and infections can progress into liver fibrosis,cirrhosis,and hepatocellular *** is no effective HCV *** this study,we rescued a recombinant PR8 influenza viral vector,called rgFLU-HCV,carrying the core and envelope glycoprotein(C/E1/E2) epitopes of HCV inserted into the influenza nonstructural protein 1 *** morphological characteristics of rgFLU-HCV and the expression of the C/E1/E2 epitopes of HCV were ***-HCV replicated in various cell lines,including MDCK,A549,and Huh7.5 *** importantly,in BALB/c mice immunized intranasally twice at a 21-day interval with 10,10,or 10 TCID rgFLU-HCV,the viral vector induced a robust antibody response to influenza and HCV and potent IFN-γ and IL-4 secretion in response to HCV antigens in a dose-dependent *** rgFLU-HCV virus also stimulated IFN-γ production by virus-specific peripheral blood mononuclear cells in patients with chronic HCV *** study demonstrated that rgFLU-HCV carrying HCV antigens is immunogenic in vivo and has potential for the development of a HCV vaccine.

关键词： Influenza virus vector HCV epitopes

PSEUDO-ALMOST PERIODIC SOLUTIONS TO SOME FUNCTIONAL DIFFERENTIAL EQUATIONS

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Annals of Differential Equations 2010年第2期26卷 215-221页

作者： Xidong sun, Yuliang Han, Baifeng Liu, xiliang Li (College of Math. and Information Sciences, Shangdong Institute of Business and Technology, Yantai 264005, Shandong, )

This paper is concerned with the pseudo-almost periodic solutions to some functional differential equations. By the exponential dichotomy theory and Schauder’s fixed-point theorem, some results on the existence and u... 详细信息

关键词： pseudo-almost periodic solutions exponential dichotomy functional differential equation Schauder’s fixed-point theorem

Construction and protective efficacy of recombinant clod-adapt influenza vaccine strain as a vector for FLU-RSV vaccine

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Construction and protective efficacy of recombinant clod-ada...

第十届全国免疫学学术大会

作者： Mengying Cai sujing sun Hongjing Gu Yueqiang Duan Chengcai Lai Li Xing Penghui Yang xiliang Wang Beijing Institute of Microbiology and Epidemiology 302 hospitial

In this sdudy we use attenuated cold-adapted influenza vaccine strains as a platform to express fitted RSV F protein and antibody *** intranasally immunized 6-8 week female BALB/c mice to evaluate the immunogenicity and protective effacy of the candidate *** use reverse genetics technology to transform the attenuated cold-adapted influenza vaccine strain NA gene by insert the RSV F epitope *** recombinant NA-F fragment were used with other seven fragments PB1,PB2,PA,HA,NA,NP and M rearranged to rescue the recombinant FLU-RSV vaccine *** use sucrose density gradient centrifugation to purify the recombinant FLU-RSV vaccine named r FLU/RSV/*** intranasal immunization we use ELISA assay to detect the level of serum Ig G,Ig G1,Ig G2a and nasal lavage lung s Ig A antibody titers,ELISPOT assay was used in the detection of IFN-γand IL-4 level in mouse spleen cell suspension,*** weeks after boost,we choose RSV A2 strains to attack the vaccine mice,and evaluate the immune protective vaccine candidates by detecting changes in body weight,lung viral load and lung *** results confirmed the identification of recombinant FLU-RSV vaccine strain has stable expression of RSV F protein and particle *** and in vitro replication assay showed r FLU/RSV/NA-F vaccine strain has shown nostatistical difference with attenuated cold-adapted H1N1 vaccine *** experiments confirmed r FLU/RSV/NA-F vaccine strain can induce RSV-specific Th1-type immune response,while effectively produce mucosal immune response,confirming that r FLU/RSV/NA-F vaccine strain has good *** virus challenge experiment results show that the candidate vaccine r FLU/RSV/NA-F can reduce nasal and lung viral load and lung *** believed that r FLU/RSV/NA-F could be a promising RSV vaccine candidate.

关键词： Respiratory syncytial virus Recombinant clod-adapt vaccine Protective

Bivalent vaccine platform based on influenza virus vaccine elicits protective immunity against respiratory syncytial virus in young and aged mice

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Bivalent vaccine platform based on influenza virus vaccine e...

第十三届全国免疫学学术大会

作者： Hongjing Gu Min Xia Linna Zhao Fang sun Jinxia Cheng Zhongpeng Zhao Penghui Yang xiliang Wang Academy of Military Medical Sciences Beijing 302 Hospital

The global burden of disease caused by respiratory syncytial virus(RSV) is increasingly recognized, not only in infants, but also in older adults(aged ≥65 years), and no vaccine licensed for prevention. In this report, we developed a cold-adapted attenuated influenza virus, termed rg HRSV-Flu ca, carrying triplex epitopes from Fusion protein in the backbone of the ca influenza vaccine neuraminidase(NA) gene using reverse genetics. Rg HRSV-Flu ca virus exhibited a cold-adapted(ca) phenotype, and its morphological characteristics were observed using electron microscopy. Moreover, BALB/c mice were immunized intranasally(i.n.) with 10, 10 or 10 TCID rg HRSV-Flu ca. Results showed a specific, robust antibody response against influenza and RSV in a dose-dependent manner. More importantly, potent humoral, mucosal and cellular immune responses protected against subsequent wild-type RSV challenges, as determined by a significant decrease in viral titers and a noticeable alleviation of histopathological alterations in the lung tissue of challenged mice. These findings demonstrate that rg HRSV-Flu ca warrants attention as a potential vaccine candidate against HRSV infection.

关键词： Triplex epitopes Fusion protein RSV Cold-adapted influenza vector

无线传感器网络基于定向扩散与分批估计的数据融合算法

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微计算机信息 2006年第9S期22卷 173-174,180页

作者：张西良孙优江苏大学机械工程学院

无线传感器网络中采集的数据存在着较大的冗余与误差,造成能量消耗过多,并且影响数据的可靠性。针对这个问题提出一种基于定向扩散与分批估计的数据融合算法,对监测同一对象的多个传感器所采集的数据进行综合,提高数据精度和可信度,并... 详细信息

无线传感器网络中采集的数据存在着较大的冗余与误差,造成能量消耗过多,并且影响数据的可靠性。针对这个问题提出一种基于定向扩散与分批估计的数据融合算法,对监测同一对象的多个传感器所采集的数据进行综合,提高数据精度和可信度,并减少数据传输量,从而降低了功耗。通过对室内环境参数(温度)的监测验证了这种算法的可行性。

关键词：无线传感器网络数据融合定向扩散分批估计

基于转录组分析的哈茨木霉thga3基因功能研究

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中国生物防治学报 2018年第1期34卷 124-132页

作者：丁洁蒋细良梅杰孙青李梅中国农业科学院植物保护研究所/农业部作物有害生物综合治理重点实验室北京100081

哈茨木霉Trichoderma harzianum Th-33的Ⅲ型Gα亚基Thga3可能参与调控木霉菌的生长、产孢、几丁质酶活性、疏水性等生物学过程。为进一步明确其功能,本研究采用Hiseq2500(Illumina)测序平台,对野生菌Th-33和thga3基因敲除突变株Δthga... 详细信息

哈茨木霉Trichoderma harzianum Th-33的Ⅲ型Gα亚基Thga3可能参与调控木霉菌的生长、产孢、几丁质酶活性、疏水性等生物学过程。为进一步明确其功能,本研究采用Hiseq2500(Illumina)测序平台,对野生菌Th-33和thga3基因敲除突变株Δthga3进行转录组测序,分析其G蛋白信号系统、细胞壁降解酶类和产孢相关基因的差异表达。与野生菌相比,Δthga3有3个G蛋白偶联受体基因发生下调表达,3个几丁质酶基因以及产孢调控基因brlA下调表达,1个G蛋白信号调控蛋白RGS1上调表达。推测thga3可能通过调控brlA的表达正调控产孢;通过调控疏水蛋白基因Tha_09745的表达调控菌丝疏水性。此外,通过调控几丁质酶基因的表达、G蛋白信号网络中其他蛋白的表达,影响菌株的拮抗和重寄生能力以及其他生物学特性。本研究为进一步探索G蛋白信号途径调控木霉菌生防特性及其机制奠定了基础。

关键词：哈茨木霉转录组测序差异表达基因 Thga3

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哈茨木霉Th-33全基因组序列测定与分析

中国生物防治学报 2016年第2期32卷 205-214页

作者：孙青蒋细良庞莉王丽荣李梅中国农业科学院植物保护研究所/农业部作物有害生物综合治理重点实验室北京100081

利用Hiseq2500高通量测序平台对哈茨木霉Th-33全基因组进行序列测定,获得196个scaffolds,共预测了10849个基因,平均长度为1776 bp(Gen Bank登录号:PRJNA272949)。以GO(gene ontology)数据库对预测出的基因做基因注释,共注释基因6238个;... 详细信息

利用Hiseq2500高通量测序平台对哈茨木霉Th-33全基因组进行序列测定,获得196个scaffolds,共预测了10849个基因,平均长度为1776 bp(Gen Bank登录号:PRJNA272949)。以GO(gene ontology)数据库对预测出的基因做基因注释,共注释基因6238个;以KEGG(kyoto encyclopedia of genes and genomes pathway database)数据库对预测出的基因做基因注释,有6789个基因注释到279条KEGG代谢途径。KEGG富集分析显示,对氨基苯甲酸甲酯降解代谢通路涉及基因最多,有232个基因;其次是双酚降解代谢通路,有206个基因。利用Rfam数据库对基因组序列进行RNA分类预测,共分为25个类别,包含7123个基因,其中涉及基因最多的为转录后修饰、蛋白质翻转和分子伴侣一类。比较了哈茨木霉、深绿木霉、绿木霉以及里氏木霉基因组中重寄生相关的碳水化合物活性酶、蛋白酶及次生代谢相关基因。研究结果有助于深入了解木霉菌的生防机制、推动木霉菌功能基因的挖掘和利用。

关键词：哈茨木霉基因组基因注释代谢通路重寄生相关基因

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棉花黄萎病病原菌大丽轮枝菌的快速分子检测

植物保护学报 2016年第6期43卷 892-899页

作者：庞莉李梅孙青王丽荣蒋细良中国农业科学院植物保护研究所北京100081

大丽轮枝菌Verticillium dahliae是引起棉花黄萎病的土传病害病原真菌。快速及时地检测出大丽轮枝菌,对棉花黄萎病的早期预警及后期防治具有重要意义。采用聚合酶链式反应(polymerase chain reaction,PCR)检测技术对已报道大丽轮枝菌的... 详细信息

大丽轮枝菌Verticillium dahliae是引起棉花黄萎病的土传病害病原真菌。快速及时地检测出大丽轮枝菌,对棉花黄萎病的早期预警及后期防治具有重要意义。采用聚合酶链式反应(polymerase chain reaction,PCR)检测技术对已报道大丽轮枝菌的检测引物进行验证、筛选和改进,获得1对改进的特异性PCR引物VDS-F/VDS-R。在优化的反应体系与扩增条件下,能特异性地从大丽轮枝菌基因组扩增出l条约520 bp的产物条带,检测灵敏度达到10^(-2)ng/μL;利用该引物可特异性地从含有大丽轮枝菌的土壤及棉花植株组织中检测出病原菌;采用巢氏PCR法对人工病土的检测灵敏度达到了10个孢子/g土。表明本引物的PCR检测体系可用于棉花黄萎病的早期快速检测。

关键词：棉花黄萎病大丽轮枝菌特异引物分子检测

时间测度链上带有时滞的中立型动力方程的伪概周期解(英文)

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应用数学 2018年第1期31卷 30-41页

作者：孙喜东刘柏枫李希亮山东工商学院数学与信息科学学院山东烟台264005

本文研究时间测度链上伪概周期函数的性质.利用时间测度链上指数型二分性性质以及Banach不动点定理,我们得到时间测度链上带有时滞的中立型动力方程的伪概周期解存在性与唯一性的充分条件.

关键词：伪概周期中立型动力方程指数型二分性时间测度