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Mitochondrial uncoupler BAM15 inhibits artery constriction and potently activates AMPK in vascular smooth muscle cells

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Acta Pharmaceutica Sinica B 2018年第6期8卷 909-918页

作者： Yu Tai Lanjun Li Xuan Peng Junxue Zhu xihai mao Nan Qin Minghui Ma Rong Huo Yunlong Bai Deli Dong Department of Pharmacology (the State-Province Key Laboratories of Biomedicine-Pharmaceutics of China Key Laboratory of Cardiovascular Research Ministry of Education) College of Pharmacy Harbin Medical University Translational Medicine Research and Cooperation Center of Northern China Heilongjiang Academy of Medical Sciences Harbin Medical University

Our previous studies found that mitochondrial uncouplers CCCP and niclosamide inhibited artery constriction and the mechanism involved AMPK activation in vascular smooth muscle cells. BAM15 is a novel type of mitochondrial uncoupler. The aim of the present study is to identify the vasoactivity of BAM15 and characterize the BAM15-induced AMPK activation in vascular smooth muscle cells(A10 cells). BAM15 relaxed phenylephrine(PE)-induced constricted rat mesenteric arteries with intact and denuded endothelium. Pretreatment with BAM15 inhibited PEinduced constriction of rat mesenteric arteries with intact and denuded endothelium. BAM15, CCCP,and niclosamide had the comparable IC50 value of vasorelaxation in PE-induced constriction of rat mesenteric arteries. BAM15 was less cytotoxic in A10 cells compared with CCCP and niclosamide.BAM15 depolarized mitochondrial membrane potential, induced mitochondrial fission, increased mitochondrial ROS production, and increased mitochondrial oxygen consumption rate in A10 cells.BAM15 potently activated AMPK in A10 cells and the efficacy of BAM15 was stronger than that of CCCP, niclosamide, and AMPK positive activators metformin and AICAR. In conclusion, BAM15 activates AMPK in vascular smooth muscle cells with higher potency than that of CCCP, niclosamide and the known AMPK activators metformin and AICAR. The present work indicates that BAM15 is a potent AMPK activator.

关键词： BAM15 Mitochondrial uncoupling AMPK Smooth muscle cells AICAR Metformin

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补肾壮筋汤多种成分的定量和定性分析

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中华中医药杂志 2024年第5期39卷 2451-2455页

作者：许云腾黄思贤施颖易周萍茆敏段辛威王丽丽李西海福建中医药大学中西医结合研究院福州350122 福建中医药大学药学院福州350122 福建省中西医结合老年性疾病重点实验室福州350122 福建中医药大学中西医结合学院福州350122

目的:建立高效液相色谱法(HPLC)对补肾壮筋汤多种活性成分进行定性定量分析。方法:冻干粉制备:制备补肾壮筋汤水提液,减压旋转浓缩,制备冻干粉。HPLC法:分析采用Agilent EC-C_(18)(4.6 mm×250 mm,5μm)色谱柱;流动相为水-乙腈,梯度洗脱... 详细信息

目的:建立高效液相色谱法(HPLC)对补肾壮筋汤多种活性成分进行定性定量分析。方法:冻干粉制备:制备补肾壮筋汤水提液,减压旋转浓缩,制备冻干粉。HPLC法:分析采用Agilent EC-C_(18)(4.6 mm×250 mm,5μm)色谱柱;流动相为水-乙腈,梯度洗脱;检测波长为212 nm(梓醇)、230 nm(松脂醇二葡萄糖苷)和240 nm(莫诺苷、芍药苷、橙皮苷),流速为1 mL/min,柱温为30℃。结果:梓醇、莫诺苷、松脂醇二葡萄糖苷、芍药苷和橙皮苷线性关系良好,R^(2)均>0.9800,平均加样回收率分别为87.63%、94.11%、89.49%、86.80%、110.34%。1 g补肾壮筋汤中药复方中,梓醇含量为5.0010 mg,莫诺苷含量为1.0340 mg,松脂醇二葡萄糖苷含量为0.0772 mg,芍药苷含量为0.4224 mg,橙皮苷含量为4.5980 mg。结论:该方法可行性好、灵敏度高,适用于补肾壮筋汤的质量控制。

关键词：高效液相色谱法补肾壮筋汤化学成分含量测定

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Clinical efficacy of low-dose emetine for patients with COVID-19:a real-world study

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Journal of Bio-X Research 2021年第2期4卷 53-59页

作者： Song Fan Qi Zhen Cheng Chen Wenjun Wang Qibing Wu Huihui Ma Chengyuan Zhang Li Zhang Baojing Lu Huiyao Ge Liang Yong Bao Li Yafen Yu Weiwei Chen Yiwen mao Guangbo Qu Li Su Aoli Wang Zhen Ding Haiwen Li Jin Zhang Yonglian Wang Yufeng Gao xihai Xu Zhongming Zhu Jun Chen Long Zhang Hongqiang Liang Song Wu Meng Huang Quan Xia Ping Li Yehuan Sun Chaozhao Liang Wei Wei Qingsong Liu Liangdan Sun Department of Urology the First Affiliated Hospital of Anhui Medical UniversityInstitute of UrologyAnhui Medical University and Anhui Province Key Laboratory of Genitourinary Diseases Department of Dermatology the First Affiliated Hospital of Anhui Medical UniversityKey Laboratory of DermatologyAnhui Medical UniversityMinistry of EducationInflammation and Immune Mediated Diseases Laboratory of Anhui Province High Magnetic Field Laboratory Key Laboratory of High Magnetic Field and Ion Beam Physical BiologyHefei Institutes of Physical ScienceChinese Academy of Sciences Department of Radiation Oncology the First Affiliated Hospital of Anhui Medical UniversityHefei Anhui Lixin County Hospital of Traditional Chinese Medicine Bozhou Department of Respiratory Medicine Anhui Feidong County People’s Hospital Center for Scientific Research of the First Affiliated Hospital of Anhui Medical University Department of Microbiology School of Basic Medical ScienceAnhui Medical University Comprehensive Lab College of BasicAnhui Medical University Department of Epidemiology and Health Statistics School of Public HealthAnhui Medical University Traditional and Western Medicine(TCM)-Integrated Cancer Center of Anhui Medical University the Department of Traditional and Western Integrative Medicine Oncologythe First Affiliated Hospital of Anhui Medical University Department of Respiratory Medicine Binhu Hospital of Hefei The Third Affiliated Hospital of Anhui Medical University the First Affiliated Hospital of Zhengzhou University The Second People’s Hospital of Hefei The Department of Infectious Diseases the First Affiliated Hospital of Anhui Medical University The Department Pulmonary and Critical Care Medicine the First Affiliated Hospital of Anhui Medical UniversityHefeiAnhui Province Anhui Suzhou Hospital SuzhouJiangsu Province Anhui Feidong County People’s Hospital Department of Pharmacy the First Affiliated Hospital of Anhui Medical University Institute of Clinical Pharmacology Anhui Medical UniversityKey Laborat

Objective:Emetine,an isoquinoline alkaloid that is enriched at high concentrations in the lung,has shown potent in vitro activity against severe acute respiratory syndrome coronavirus 2(SARS-CoV-2).The aim of this study was to better understand the effectiveness of low-dose emetine for patients with coronavirus disease 2019(COVID-19).Methods:In this real-world study,63 patients with mild or common COVID-19 were recruited from Wuhan Fangcang Shelter Hospital and five COVID-19-designated hospitals in Anhui Province,China from February to March 2020.Thirty-nine patients from Wuhan Fangcang Shelter Hospital were assigned to a pragmatic randomized controlled clinical trial,and 24 patients from the 5 COVID-19-designated hospitals in Anhui Province underwent a real-world study.The medication course of emetine was less than 10 days.The main symptoms and adverse reactions of all patients were observed and recorded.The primary outcome measure was the time required for a negative SARS-CoV-2 RNA result or the negative result rate on day 10.Secondary outcomes included axillary temperature,transcutaneous oxygen saturation,and respiratory frequency recovery.The study was approved by the Ethics Committee of The First Affiliated Hospital of Anhui Medical University on February 20,2019(approval No.PJ2020-03-19)and was registered with the Chinese Clinical Trial Registry on February 20,2019(registration number:ChiCTR2000030022).Results:The oxygen saturation values were higher in the treatment group than in the control group on the first day after enrollment for patients treated at Fangcang Shelter Hospital.The axillary body temperature,respiratory rate,and oxygen saturation among patients in Fangcang Shelter Hospital were related to the time effect but not to the intervention measures.The respiratory rate and oxygen saturation of patients in the Anhui designated hospitals were related to the intervention measures but not to the time effect.The axillary body temperature of patients in Anhui designated hospitals was related to the tim

关键词： arbidol COVID-19 emetine randomized controlled clinical trial real-world study

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用海水与海水晶淡化养殖南美白对虾的效果对比试验

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水产科技 2005年第3期32卷 33-35页

作者：梁锡海莫振明茂名市淡水良种繁殖试验场茂名市海洋与渔业环境监测站

本文较详细地介绍了利用海水与海水晶进行20亩池塘的淡化养殖南美白对虾的效果对比试验情况。使用海水淡化养殖的池塘,虾苗淡化成活率90.3%,平均亩产364.7kg,养殖成活率 90.2%,亩毛利润为5616.2元;使用海水晶淡化养殖的池塘,虾苗淡化成... 详细信息

本文较详细地介绍了利用海水与海水晶进行20亩池塘的淡化养殖南美白对虾的效果对比试验情况。使用海水淡化养殖的池塘,虾苗淡化成活率90.3%,平均亩产364.7kg,养殖成活率 90.2%,亩毛利润为5616.2元;使用海水晶淡化养殖的池塘,虾苗淡化成活率87.6%,平均亩产 353.4kg,养殖成活率88.7%,亩毛利润为5441.6元。试验结果表明,两者在养殖成活率、生长速度、亩产量、饲料系数等方面均表现出无明显的差别。本文还介绍了淡化养殖南美白对虾操作技术, 对养殖生产者具有一定的参考价值。

关键词：海水海水晶淡化养殖南差白对虾对比试验南美白对虾养殖成活率虾苗淡化亩产量生长速度

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中西医结合研究生医学实验方法与技术课程体系构建及其科研能力培养的实践

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中医教育 2021年第1期40卷 8-11页

作者：毛敬洁陈银秀林久茂李西海褚剑锋彭军福建中医药大学中西医结合研究院福州350122 福建省中西医结合老年性疾病重点实验室福州350122 福建中医药大学研究生部福州350122

研究生教育是培养高级医学人才的重要组成部分,在医学教育研究领域占有重要地位。在中西医结合研究生的培养过程中,医学实验方法和技能的训练对医学研究生科研素养培育至关重要。从教学理念模式、课程设计内容、成绩评价以及科研素养培... 详细信息

研究生教育是培养高级医学人才的重要组成部分,在医学教育研究领域占有重要地位。在中西医结合研究生的培养过程中,医学实验方法和技能的训练对医学研究生科研素养培育至关重要。从教学理念模式、课程设计内容、成绩评价以及科研素养培育等方面,总结了中医药大学医学实验方法与技术课程体系构建及其研究生科研能力培养中的教学实践和思考体会,探索出合理的中西医结合研究生培养模式,为切实提高中西医结合人才培养质量奠定基础。

关键词：研究生培养医学实验教学中西医结合

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