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化学学报 2003年第3期61卷 331-335页

作者：谢修银王志勇刘欲文汪存信武汉大学化学与分子科学学院武汉430072

在 310 15K ,pH =7 0的 0 1mol·L-1Na2 HPO4 NaH2 PO4 缓冲溶液中 ,利用热动力学方法研究了L 抗坏血酸 ,Cu2 +及二者同时存在时 ,过氧化氢酶催化H2 O2 分解反应的动力学规律 .发现L 抗坏血酸和Cu2 +单独存在时对酶反应没有明显的抑... 详细信息

在 310 15K ,pH =7 0的 0 1mol·L-1Na2 HPO4 NaH2 PO4 缓冲溶液中 ,利用热动力学方法研究了L 抗坏血酸 ,Cu2 +及二者同时存在时 ,过氧化氢酶催化H2 O2 分解反应的动力学规律 .发现L 抗坏血酸和Cu2 +单独存在时对酶反应没有明显的抑制作用 ,二者共存时 ,对反应有非线性抑制作用 .在一定的酶和底物浓度下 ,L 抗坏血酸和Cu2 +不影响总反应的一级速率方程的形式 ,只减小了一级反应速率常数 .酶活性随抑制剂浓度变化关系呈S形曲线 .结合实验结果和文献 ,提出了一种L 抗坏血酸和Cu2

关键词：过氧化氢酶 L-抗坏血酸热动力学协同抑制二价铜离子抗氧化剂

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漆酶与各种类型底物反应性能的微量热法研究

华中师范大学学报（自然科学版） 1999年第2期33卷 246-249页

作者：谢修银颜承农吴鼎泉屈松生荆州师范学院化学系荆州434104 武汉大学化学学院武汉430072

在厌氧条件下．用LKB－2107Batch微量热系统研究了在298．15K,0．1mol／L磷酸盐缓冲溶液（pH7．4）中谈树漆酶与5种废物的反应．测定了各反应的反应焓变△rHm，米氏常数Km，酶底复合过程的吉布斯自由能上△Go,复合物中电子转移速率常数... 详细信息

在厌氧条件下．用LKB－2107Batch微量热系统研究了在298．15K,0．1mol／L磷酸盐缓冲溶液（pH7．4）中谈树漆酶与5种废物的反应．测定了各反应的反应焓变△rHm，米氏常数Km，酶底复合过程的吉布斯自由能上△Go,复合物中电子转移速率常数k_2，以及漆酶对各种废物的催化活性EA讨论了在相同的实验条件下，各种酶底复合物的稳定性，底物官能团及其位置对酶反应中的电子转移速率和非酶反应中自由基聚合形式及产物结构的影响．

关键词：漆树漆酶微量热法催化反应电子转移

SDS水相胶束体系中酶促反应的热动力学研究

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长江大学学报（自然科学版） 2004年第1期1卷 70-73页

作者：谢修银长江大学化学与环境工程学院 434020

　在298.15K十二烷基硫酸钠(SDS)的水相胶束体系中,利用微量热方法研究了辣根过氧化物酶(HRP)催化H2O2氧化邻苯二胺(OPD)的反应.SDS作为一种蛋白质变性剂,不仅能够使HRP变性失去部分活性,而且还能使HRP对底物OPD的米氏常数Km(OPD)和最... 详细信息

　在298.15K十二烷基硫酸钠(SDS)的水相胶束体系中,利用微量热方法研究了辣根过氧化物酶(HRP)催化H2O2氧化邻苯二胺(OPD)的反应.SDS作为一种蛋白质变性剂,不仅能够使HRP变性失去部分活性,而且还能使HRP对底物OPD的米氏常数Km(OPD)和最大反应速率都比其在缓冲体系中小;而在SDS溶胶中,OPD相对过量时,HRP对底物H2O2不符合米氏机理.

关键词：胶束体系 SDS 动力学研究酶促反应水相十二烷基硫酸钠辣根过氧化物酶 H2O2 HRP 邻苯二胺缓冲体系反应速率米氏常数 15K 变性剂蛋白质微量热底物

STUDIES OF THE REACTION BETWEEN LACCASE AND p-PHENYLENEDIAMIME BY MICROCALORIMETRY

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Wuhan University Journal of Natural Sciences 1998年第1期3卷 107-107页

作者： xie xiuyin Yan Chengnong Wu Dingquan Qu Shongsheng Du Yumin Wuhan University Department of Chemistry Wuhan China (GRID:grid.49470.3e) (ISNI:***) Wuhan University Department of Environment Science Wuhan China (GRID:grid.49470.3e) (ISNI:***)

The molar reation enthalpy,the Michaelis constant and the observed rate constant of the reaction between the Rhus vernicifera laccase and p-phenylenediamine have been determined at 298. 15 K by LKB-2107 microcalorimetry system in 0.1 mol/L phosphate salt buffer (pH7. 4) to be △rHm=-136.36±0. 36kJ/mol, Km= 5. 58×10-3 mol/L and k1 =8. 63×10-3s-1, respectively. The catalyst activity of laccase withp-phenylenediamine as substrate has been determined to be EA=0. 045 IU in the experimental *** observed activation energy of non-enzymic step of the reaction, the Gibbs binding energy of the combination process of laccase and substrate have been also calculated. The physical significance of the determined parameters were discussed for different step of the reaction.

关键词： microcalorimetry rhus vernicifera laccase p-phenylenediamine enzymatic reaction

合并重症肺炎、脑炎、多器官功能障碍的重症恙虫病1例

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中华全科医学 2023年第11期21卷 1990-1994页

作者：谢秀银王库良谢友玲韦晴霏广西医科大学第四附属医院全科医学科广西柳州545000

恙虫病是一种由恙虫病东方体引起的急性自然疫源性传染病,为目前最易漏诊的传染病之一,以突然起病、高热、皮肤焦痂或溃疡、淋巴结肿大为特征,基础病理改变为血管炎及血管周围炎,临床表现为不同程度的器官功能损害、功能坏死甚至衰竭。... 详细信息

恙虫病是一种由恙虫病东方体引起的急性自然疫源性传染病,为目前最易漏诊的传染病之一,以突然起病、高热、皮肤焦痂或溃疡、淋巴结肿大为特征,基础病理改变为血管炎及血管周围炎,临床表现为不同程度的器官功能损害、功能坏死甚至衰竭。本文报道以发热为首发症状,合并重症肺炎、脑炎、多器官功能障碍的重症恙虫病患者1例,经治疗后痊愈出院。本文就此病例进行报告及文献复习,旨在提高临床对恙虫病的认识,做到早诊断、早治疗,个体化治疗。

关键词：恙虫病重症恙虫病重症肺炎脑炎多器官功能障碍

Characteristics of gut microbiota dysbiosis in patients with colorectal polyps

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World Journal of Gastrointestinal Oncology 2025年第1期17卷 13-21页

作者： Xian-Rong Wu Xiao-Hong He Yong-Fang xie School of Life Health Information Science and Engineering Chongqing Post and Communications UniversityChongqing 400065China

This editorial,inspired by a recent study published in the World Journal of Gastrointestinal Oncology,covers the research findings on microbiota changes in various *** recurrent colorectal polyps,the abundances of Klebsiella,Parvimonas,and Clostridium increase,while those of Bifidobacterium and Lactoba-cillus *** dysbiosis may promote the formation and recurrence of *** microbial changes have also been observed in colorectal cancer,inflammatory bowel disease,autism spectrum disorder,and metabolic syndrome,indicating the role of increased pathogens and decreased probiotics in these *** the gut microbiota,particularly by increasing probiotic levels,may help prevent polyp recurrence and promote gut *** microbial intervention strategy holds promise as an adjunctive treatment for patients with colorectal polyps.

关键词： Recurrent colorectal polyps Gut microbiota dysbiosis Klebsiella Probiotics Intestinal inflammation Microbial intervention strategy

FK506 contributes to peripheral nerve regeneration by inhibiting neuroinflammatory responses and promoting neuron survival

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Neural Regeneration Research 2025年第7期20卷 2108-2115页

作者： Yuhui Kou Zongxue Jin Yusong Yuan Bo Ma Wenyong xie Na Han Department of Trauma and Orthopedics Peking University People’s HospitalBeijingChina National Center for Trauma Medicine Peking University People’s HospitalBeijingChina Key Laboratory of Trauma and Neural Regeneration(Peking University) Ministry of EducationBeijingChina Trauma Medicine Center Peking University People’s HospitalBeijingChina Department of Orthopedics China-Japan Friendship HospitalBeijingChina

FK506(Tacrolimus)is a systemic immunosuppressant approved by the *** and Drug ***506 has been shown to promote peripheral nerve regeneration,however,its precise mechanism of action and its pathways remain *** this study,we established a rat model of sciatic nerve injury and found that FK506 improved the morphology of the injured sciatic nerve,increased the numbers of motor and sensory neurons,reduced inflammatory responses,markedly improved the conduction function of the injured nerve,and promoted motor function *** findings suggest that FK506 promotes peripheral nerve structure recovery and functional regeneration by reducing the intensity of inflammation after neuronal injury and increasing the number of surviving neurons.

关键词： FK506 inflammation motor neurons nerve regeneration neuron peripheral nerve injury sensory neurons

Stiffness-tunable biomaterials provide a good extracellular matrix environment for axon growth and regeneration

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Neural Regeneration Research 2025年第5期20卷 1364-1376页

作者： Ronglin Han Lanxin Luo Caiyan Wei Yaru Qiao Jiming xie Xianchao Pan Juan Xing Department of Pathophysiology School of Basic Medical SciencesSouthwest Medical UniversityLuzhouSichuan ProvinceChina Department of Medicinal Chemistry School of PharmacySouthwest Medical UniversityLuzhouSichuan ProvinceChina

Neuronal growth, extension, branching, and formation of neural networks are markedly influenced by the extracellular matrix—a complex network composed of proteins and carbohydrates secreted by cells. In addition to providing physical support for cells, the extracellular matrix also conveys critical mechanical stiffness cues. During the development of the nervous system, extracellular matrix stiffness plays a central role in guiding neuronal growth, particularly in the context of axonal extension, which is crucial for the formation of neural networks. In neural tissue engineering, manipulation of biomaterial stiffness is a promising strategy to provide a permissive environment for the repair and regeneration of injured nervous tissue. Recent research has fine-tuned synthetic biomaterials to fabricate scaffolds that closely replicate the stiffness profiles observed in the nervous system. In this review, we highlight the molecular mechanisms by which extracellular matrix stiffness regulates axonal growth and regeneration. We highlight the progress made in the development of stiffness-tunable biomaterials to emulate in vivo extracellular matrix environments, with an emphasis on their application in neural repair and regeneration, along with a discussion of the current limitations and future prospects. The exploration and optimization of the stiffness-tunable biomaterials has the potential to markedly advance the development of neural tissue engineering.

关键词： alginate axon growth biomaterials extracellular matrix neural repair neurons neuroregeneration polyacrylamide polydimethylsiloxane stiffness

Traditional Chinese medicine in the treatment of Helicobacter pylorirelated gastritis:The mechanisms of signalling pathway regulations

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World Journal of Gastroenterology 2025年第3期31卷 11-28页

作者： Pei-Pei Zhang Liang Li Hao-Yu Qu Guang-Yu Chen Meng-Zhou xie Yan-Kun Chen School of Traditional Chinese Medicine Hunan University of Chinese MedicineChangsha 410200Hunan ProvinceChina Hunan Engineering Technology Research Center for Medicinal and Functional Food Hunan University of Chinese MedicineChangsha 410200Hunan ProvinceChina Key Laboratory of Traditional Chinese Medicine Heart and Lung Syndrome Differentiation and Medicated Diet and Dietotherapy Hunan University of Chinese MedicineChangsha 410200Hunan ProvinceChina School of Informatics Hunan University of Chinese MedicineChangsha 410208Hunan ProvinceChina Precision Medicine Research and Development Center Zhuhai Institute of Advanced TechnologyChinese Academy of SciencesZhuhai 519000Guangdong ProvinceChina

Helicobacter pylori-associated gastritis(HPAG)is a common condition of the gastrointestinal ***,extensive and long-term antibiotic use has resulted in numerous adverse effects,including increased resistance,gastrointestinal dysfunction,and increased recurrence *** these concerns develop,traditional Chinese medicine(TCM)may have *** is based on the concept of completeness and aims to eliminate pathogens and strengthen the *** has the potential to prevent this condition while also boosting the rate of Helicobacter pylori *** review elaborates on the mechanism of TCM treatment for HPAG based on cellular signalling pathways,which reflects the flexibility of TCM in treating diseases and the advantages of multi-level,multipathway,and multi-target treatments for HPAG.

关键词： Helicobacter pylori Helicobacter pylori-associated gastritis Traditional Chinese medicine Signalling pathway Eliminate pathogens Strengthen the body