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Impact of Plant Density on the Formation of Potato Mimitubers Derived from Microtubers and Tip-Cuttings in Plastic Houses

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Journal of Integrative Agriculture 2013年第6期12卷 1008-1017页

作者： JIN Hui LIU Jun SONG Bo-tao xie cong-hua Key Laboratory of Horticultural Plant Biology Ministry of Education/Potato Engineering Research Center of Hubei Province Huazhong Agricultural University

The potato minitubers have been widely used for the elite seed propagation to improve the seed potato system in China. However, little information is available for an efficient production of the minitubers with high plant density in the protected growing conditions like plastic houses. In present research, the minitubers of a wide-grown potato variety, Favorita, were produced with the microtubers from tissue culture and the tip-cuttings of the microtuber plants. Three plant densities, 200, 400 and 600 microtubers or plants m-2 were set up with the randomized block design of 3 replications and the experiment was repeated in 4 seasons in 2009-2010. The canopy development, light interception, dry weight production and partitioning, tuberization and tuber weight were investigated to elucidate the mechanism by which the plant density affects the formation and growth of the minitubers. The results showed that the number of the tubers formed per unite area was in line with the increase in plant density. The difference in leaf area index （LAI） between the plant densities, especially in early stage of the plant growth, resulted in more radiation interception and dry weight producing in higher plant density than in lower one. However, our analysis demonstrated that the conversion coefficient of the cumulative intercepted radiation to plant weight and the dry weight partition rate to the tubers were constant between plant densities, suggesting that less amount of the photoassimilates partitioned to individual tubers is causal for more small tubers in high plant density. A negative exponential curve model, determined by total number of tubers produced per unit area and the mean tuber weight, fitted well to the tuber size distribution pattern. The optimum plant density could be estimated from this model for a maximum production of the minitubers with desired size.

关键词： potato minituber microtuber tip-cutting plant density

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Research on Algorithm for Mining Negative Association Rules Based on Frequent Pattern Tree

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Wuhan University Journal of Natural Sciences 2006年第1期11卷 37-41页

作者： ZHU Yu-quan YANG He-biao SONG Yu-qing xie cong-hua School of Computer Science and Communication Engineering Jiangsu University Zhenjiang 212013 JiangsuChina

Typical association rules consider only items enumerated in transactions. Such rules are referred to as positive association rules. Negative association rules also consider the same items, but in addition consider negated items （i. e. absent from transactions）. Negative association rules are useful in market-basket analysis to identify products that conflict with each other or products that complement each other. They are also very convenient for associative classifiers, classifiers that build their classification model based on association rules. Indeed, mining for such rules necessitates the examination of an exponentially large search space. Despite their usefulness, very few algorithms to mine them have been proposed to date. In this paper, an algorithm based on FP tree is presented to discover negative association rules.

关键词： data mining FP-tree Negative Association Rules

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Population Improvement of Resistance to Late Blight in Tetraploid Potato:A Case Study in Combination with AFLP Marker Assisted Background Selection

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Agricultural Sciences in China 2011年第8期10卷 1177-1187页

作者： YAO Chun-guang SONG Bo-tao LIU Jun WU Cheng-jin CHENG Qun LI Da-chun xie cong-hua Key Laboratory of Horticultural Plant Biology Ministry of Education/National Center for Vegetable Improvement （Central China）/Potato Engineering and Technology Research Center of Hubei Province/Huazhong Agricultural UniversityWuhan 430070P.R.China Southern Potato Research Center of China/Enshi Academy of Agricultural Sciences Enshi 445000P.R.China

Cultivated potato with high level of horizontal resistance against late blight is one of the most important goals of potato *** recurrent selection has been adopted to increase the level of potato horizontal resistance and a B3C1 population without R1-R11 dominant genes has been released by the International Potato Center at the short-day condition of *** present research was carried out to further improve the resistance and the agronomic traits of B3C1 population under long-day condition of Hubei,China,with maximized retention of its genetic ***-seven individual clones of B3C1 were used to generate population B3C2 by in-population crossing with the bulk pollens aiming to elevate the frequency of late blight resistance genotypes and to improve the adaptation to local long-day *** late blight resistance and the main agronomic traits including the maturity,the plant characters and the tuber traits were evaluated for the foreground selection in three years,by which 130 pedigrees were maintained as the basic population of B3C2 for further selection.A total of 312 polymorphic loci detected by 9 AFLP marker combinations were used to monitor the genetic diversity of the populations for the background *** B3C2 population of 51 clones was finally selected,of which the frequency of resistant genotypes increased by 23.8% points and the genetic diversity was maintained by about 96% as referred to *** results strongly suggested that combination of the foreground selection for target traits and the background selection for the genetic diversity is an efficient strategy in the recurrent selection of tetraploid potato to improve quantitative traits.

关键词： potato recurrent selection late blight horizontal resistance genetic diversity

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马铃薯蛋白激酶基因StPK1启动子的克隆及活性分析

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中国农业科学 2011年第5期44卷 867-873页

作者：吴田谢从华西南林业大学园林学院华中农业大学园艺林学学院/园艺植物生物学教育部重点实验室/国家蔬菜改良中心华中分中心/湖北省马铃薯工程技术研究中心

【目的】研究马铃薯蛋白激酶基因StPK1,为在马铃薯抗病反应中的功能提供证据。【方法】利用TAIL-PCR技术,从马铃薯晚疫病水平抗性、垂直抗性和感病等3种材料中,分别克隆了该基因的启动子区域。此外,将从水平抗性材料中克隆得到的启动子... 详细信息

【目的】研究马铃薯蛋白激酶基因StPK1,为在马铃薯抗病反应中的功能提供证据。【方法】利用TAIL-PCR技术,从马铃薯晚疫病水平抗性、垂直抗性和感病等3种材料中,分别克隆了该基因的启动子区域。此外,将从水平抗性材料中克隆得到的启动子与GUS连接构建植物表达载体,通过农杆菌介导的遗传转化法转化本氏烟(Nicotiana benthamiana),将得到的转基因烟草分别用水、晚疫病原菌或水杨酸(SA)溶液处理,并进行表达活性分析。【结果】3个启动子长度分别为922、929和922 bp,均具有TATA-box和CAAT-box以及多种与抗病反应和基因时空表达有关的元件,而三者的主要差异是个别碱基的不同。GUS组织化学染色表明,用晚疫病原菌或SA处理的转基因烟草被染成蓝色。【结论】StPK1启动子为有活性的启动子,且为病原和SA诱导型启动子。

关键词：马铃薯晚疫病 StPK1启动子 TAIL-PCR

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成软骨细胞瘤骨膜反应的影像特点

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中国医学影像技术 2008年第2期24卷 264-266页

作者：谢元忠谢丛华武汉大学中南医院放化疗科湖北武汉430071

目的分析成软骨细胞瘤骨膜反应的X线及CT表现特点,探讨其诊断价值。方法对39例成软骨细胞瘤的X线平片及CT资料进行回顾性分析研究,重点分析其骨膜反应的部位、形态及范围。结果39例中24例出现骨膜反应,在平片上8例位于干骺端的两侧,CT... 详细信息

目的分析成软骨细胞瘤骨膜反应的X线及CT表现特点,探讨其诊断价值。方法对39例成软骨细胞瘤的X线平片及CT资料进行回顾性分析研究,重点分析其骨膜反应的部位、形态及范围。结果39例中24例出现骨膜反应,在平片上8例位于干骺端的两侧,CT上见环绕骨皮质周围,较广泛。5例骨膜反应与皮质断裂处有一间隔距离;3例位于皮质断裂处的对侧。5例骨膜反应很广泛,在CT上呈不规则的广泛花边状改变。7例伴有周围软组织肿胀。结论成软骨细胞瘤骨膜反应广泛,形态多样,与病灶部位及病灶的良性形态不相称;这一征象对本病的诊断与鉴别诊断具有重要价值。

关键词：成软骨细胞瘤骨膜反应放射学

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4种信号分子处理后马铃薯Star基因表达的RT-PCR分析

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华中农业大学学报 2010年第3期29卷 268-271页

作者：吴田谢从华西南林学院园林学院昆明650224 华中农业大学园艺林学学院武汉430070

以马铃薯叶片为试验材料,其叶片分别经外源SA、MJ、ETH、ABA处理后,在不同时间点取样,提取总RNA,对Star基因的表达进行半定量RT-PCR分析。结果表明,Star基因能够被SA、MJ、ABA迅速诱导表达,而被ETH诱导表达的时间比较晚。可见,Star基因... 详细信息

以马铃薯叶片为试验材料,其叶片分别经外源SA、MJ、ETH、ABA处理后,在不同时间点取样,提取总RNA,对Star基因的表达进行半定量RT-PCR分析。结果表明,Star基因能够被SA、MJ、ABA迅速诱导表达,而被ETH诱导表达的时间比较晚。可见,Star基因被4种信号分子诱导表达之间存在广泛的重叠,反映了在马铃薯抵御晚疫病菌侵染过程中Star基因参与了一个非常复杂的信号传递过程。

关键词：马铃薯富含锚蛋白重复结构域晚疫病基因表达

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Histiocytic sarcoma of tonsil:a case report

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蚌埠医学院学报 2012年第10期37卷 1270-1272页

作者： QIN Dan-mei xie cong-hua WU Qiu-ji ZHANG Jun-hong Department of Oncology The Traditional Chinese MedicineHospital of Hubei Wuhan Hubei 430000 Department of Radiation and Medical Oncology Zhongnan Hospital WuhanUniversity Wuhan Hubei 430071

In this article,a case of histiocytic sarcoma(HS) arising from tonsil in a 60 year-old male patient was *** a local disease,a regime consisting of 4 cycles of(CHOP) associated with radiotherapy was used.A positive result was gained after *** HS is a rare hematopoietic neoplasm derived from hematopoietic monoblasts with aggressive characteristic and poor *** of disease and tumor size are possibly associated with the prognosis.

关键词： histiocytic sarcoma tonsil CHOP

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Identification of QTLs Related to Bolting in Brassica rapa ssp. pekinensis (syn. Brassica campestris ssp. pekinensis)

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Agricultural Sciences in China 2006年第4期5卷 265-271页

作者： ZHANG Xiao-wei WU Jian ZHAO Jian-jun SONG Xiao-fei LI Ying ZHANG Yan-guo XU Dong-hui SUN Ri-fei YUAN Yu-xiang xie cong-hua WANG Xiao-wu College of Horticulture and Forestry Science Huazhong Agricultural University Wuhan 430070 P.R.China Institute of Vegetables and Flowers. Chinese Academy of Agriculture Sciences Beijing 100081 P.R.China Institute of Biotechnology Henan Academy of Agricultural Sciences Zhengzhou 450002 P.R.China Life Science College Hebei Agricultural University Baoding 071101 P.R.China

A genetic linkage map of Brassica rapa ssp. pekinensis was constructed with 186 AFLP （amplified fragment length polymorphism） markers by using a doubled-haploid （DH） population with 183 individuals. The individuals were derived from F1 which was developed by crossing a bolting resistant DH line Y-177-12 and an easy bolting DH line Y195-93a. AFLPs were generated by the use of restriction enzymes EcoR Ⅰ and Mse Ⅰ . The segregation of each marker and linkage was analyzed by using JoinMap version 3.0. Mapped markers were aligned in ten linkage groups which covered 887.8 cM with an average marker interval of 4.47 cM. Markers showing skewed segregation ratio were clustered in six LGs. Quantitative trait loci （QTL） were mapped for bolting resistance by using MAPQTL 4.0 package. Four QTLs explaining from 7.0 to 9.4% of the total variation were detected, all of them increase bolting resistance. These mapped QTLs could be used to develop a marker assisted selection programme for bolting resistance breeding.

关键词： bolting QTL linkage map Chinese cabbage

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Capecitabine combined with weekly docetaxel in Chinese patients 〉65 years with anthracycline-resistant metastatic breast cancer

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Chinese Medical Journal 2010年第22期123卷 3212-3216页

作者： WANG hua-qing QIAN Zheng-zi LIU Xian-ming ZHANG Hui-lai LI Lan-fang QIU Li-hua HOU Yun ZHOU Shi-yong HAO Xi-shan xie cong-hua Department of Medical Oncology Tianjin Medical University Cancer Hospital and Institute Key Laboratory of Breast Cancer Prevention and Therapy Ministry of Education Key Laboratory of Cancer Prevention and Therapy Tianjin 300060 China Department of Radiation and Medical Oncology Zhongnan Hospital Hubei Cancer Clinical Study Center Wuhan University Wuhan Hubei 430071 China

Background There are no data on more tolerable capecitabine doses in elderly patients in Chinese population. The aim of this study was to evaluate the activity and safety of capecitabine combined with weekly docetaxel for the treatment of anthracycline-resistant metastatic breast cancer （MBC） in older Chinese patients. Methods MBC patients aged 〉65 years pretreated with 1-5 prior chemotherapy regimens, including an anthracycline, received oral capecitabine 825 mg/m^2 twice daily, days 1-14, plus docetaxel 30 mg/m^2 on days 1 and 8 every 21 days. All 41 enrolled patients received at least 1 dose of treatment and were evaluable for safety; 38 received at least 2 cycles （median 4, range 2-8） and were evaluable for efficacy. Results The overall objective response rate was 47%, including complete responses in 8% of patients. Median time to progression was 8.9 months. Median overall survival was 17.6 months. The most common side effects were haematological and gastrointestinal toxicities and hand-foot syndrome. The only grade 3/4 adverse events were neutropenia （12%）, alopecia （7%）, grade 3 nausea and vomiting （2%） and grade 3 nail toxicity （2%）. Conclusions Capecitabine 825 mg/m^2 twice daily plus weekly docetaxel is active with an acceptable safety profile in Chinese women 〉65 years with anthracycline-resistant MBC. Efficacy and tolerability compare favourably with previously reported trials evaluating higher capecitabine doses in combination with 3-weekly or weekly docetaxel.

关键词： capecitabine weekly docetaxel metastatic breast cancer anthracycline elderly tolerability

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马铃薯class Ⅰ patatin基因在试管块茎形成中的功能

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作物学报 2006年第9期32卷 1406-1409页

作者：司怀军柳俊谢从华国家蔬菜改良中心华中分中心/园艺植物生物学教育部重点实验室/华中农业大学

将正反义classⅠpatatin基因导入马铃薯品种甘农薯2号中,有2个转正义基因株系试管块茎的可溶性蛋白含量和LAH活性与对照相比有不同程度的增加,有3个转反义基因株系的可溶性蛋白质含量下降,并且所有转反义基因植株的LAH活性都不同程度地... 详细信息

将正反义classⅠpatatin基因导入马铃薯品种甘农薯2号中,有2个转正义基因株系试管块茎的可溶性蛋白含量和LAH活性与对照相比有不同程度的增加,有3个转反义基因株系的可溶性蛋白质含量下降,并且所有转反义基因植株的LAH活性都不同程度地降低。试管块茎的诱导结果表明,有1个转正义基因株系的结薯株率和单株结薯数比其对照明显增加,有2个转反义基因株系的结薯株率和单株结薯数比对照明显减少,说明该classⅠpatatin基因参与了马铃薯试管块茎的形成及其调控。

关键词：马铃薯 class Ⅰ patatin基因转化块茎形成

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