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Application of Next Generation Sequencing for Rapid Identification of Lactic Acid Bacteria

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Asian Agricultural Research 2024年第4期16卷 27-32页

作者： xiaxia HOU Yunxia WANG Shuhuan ZHAO Hongbing JIA Cuizhi li Inner Mongolia Yili Industrial Group Co. Ltd.Hohhot 010110China

The rapid identification of lactic acid bacteria,which are essential microorganisms in the food industry,is of great significance for industrial applications.The identification of lactic acid bacteria traditionally relies on the isolation and identification of pure colonies.While this method is well-established and widely used,it is not without limitations.The subjective judgment inherent in the isolation and purification process introduces potential for error,and the incomplete nature of the isolation process can result in the loss of valuable information.The advent of next generation sequencing has provided a novel approach to the rapid identification of lactic acid bacteria.This technology offers several advantages,including rapidity,accuracy,high throughput,and low cost.Next generation sequencing represents a significant advancement in the field of DNA sequencing.Its ability to rapidly and accurately identify lactic acid bacteria strains in samples with insufficient information or in the presence of multiple lactic acid bacteria sets it apart as a valuable tool.The application of this technology not only circumvents the potential errors inherent in the traditional method but also provides a robust foundation for the expeditious identification of lactic acid bacteria strains and the authentication of bacterial powder in industrial applications.This paper commences with an overview of traditional and molecular biology methods for the identification of lactic acid bacteria.While each method has its own advantages,they are not without limitations in practical application.Subsequently,the paper provides an introduction of the principle,process,advantages,and disadvantages of next generation sequencing,and also details its application in strain identification and rapid identification of lactic acid bacteria.The objective of this study is to provide a comprehensive and reliable basis for the rapid identification of industrial lactic acid bacteria strains and the authenticity identification of bacterial powder.

关键词： Lactic acid bacteria Rapid identification Next generation sequencing

Advances in the Use of Prone Cardiopulmonary Resuscitation During Spinal Surgery in the Prone Position

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Journal of Clinical and Nursing Research 2024年第4期8卷 316-321页

作者： Ruigang Zhu xiaxia Chen Jing Wang Miaocui li Xiaolin Wang The Affiliated Hospital of Qingdao University Pingdu 266700China

Spinal surgery is usually performed in the prone position, which is a longer and more difficult procedure and is prone to complications such as circulatory dysfunction and stress injuries. Among them, stress injury is the main complication of prone spine surgery, but the reasons for stress injury in prone spine surgery are not clear, and whether prone cardiopulmonary resuscitation (CPR) can be used needs to be further verified. Supine cardiopulmonary resuscitation is commonly used in posterior spinal surgery, retroperitoneal surgery, and so on, which can effectively improve the patient’s hypoxemia. Such surgeries require a high level of anesthetic management, and cardiopulmonary resuscitation is necessary if a patient in a prone position experiences cardiac arrest. In the process of cardiopulmonary resuscitation, supine cardiopulmonary resuscitation is often used, especially for some obese patients, if they are immediately changed to the supine position, it takes up more time, there may be wound infection, and there is a possibility of missing the optimal rescue and resuscitation time. Based on this, this paper reviews the use of prone-position cardiopulmonary resuscitation for spinal surgery in the prone position.

关键词： Prone position Spinal surgery Cardiopulmonary resuscitation Research progress

Applications of M_xSe_y(M=Fe, Co, Ni) and Their Composites in Electrochemical Energy Storage and Conversion

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Nano-Micro Letters 2019年第3期11卷 46-78页

作者： Huijie Zhou xiaxia li Yan li Mingbo Zheng Huan Pang School of Chemistry and Chemical Engineering Guangling College Yangzhou University

Transition-metal selenides(M_xSe_y,M = Fe,Co,Ni)and their composites exhibit good storage capacities for sodium and lithium ions and occupy a unique position in research on sodium-ion and lithium-ion batteries.MxSey and their composites are used as active materials to improve catalytic activity.However,low electrical conductivity,poor cycle stability,and low rate performance severely limit their applications.This review provides a comprehensive introduction to and understanding of the current research progress of M_xSe_y and their composites.Moreover,this review proposes a broader research platform for these materials,including various bioelectrocatalytic performance tests,lithium-sulfur batteries,and fuel cells.The synthesis method and related mechanisms of M_xSe_y and their composites are reviewed,and the effects of material morphologies on their electrochemical performance are discussed.The advantages and disadvantages of M_xSe_y and their composites as well as possible strategies for improving the storage and conversion of electrochemical energy are also summarized.

关键词： M_xSe_y Battery Supercapacitor Electrocatalysis

Nanoreactors derived from silica-protection-assisted metal-organic framework

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Chinese Chemical Letters 2020年第9期31卷 2207-2210页

作者： ling Jin xiaxia li Chunsen liu Huan Pang School of Chemistry and Chemical Engineering Guangling CollegeYangzhou UniversityYangzhou 225009China Henan Provincial Key Laboratory of Surface&Interface Science Zhengzhou University of Light IndustryZhengzhou 450002China

The construction of highly stable and regular nanoreactors is a major challenge.In this work,we use a facile template protection method to obtain ZIF-67@SiO2(JS) and to encapsulate metal oxide nanoparticles(Co3O4) into nanoreactors(SiO2).ZIF-67 crystals provide a cobalt species;SiO2 was first used as a protective layer of ZIF-67 and then as a nanoreactor for metastable metal oxide nanoparticles.On this basis,Co3O4@SiO2 with dodecahedron morphology were synthesized by calcining JS at different tempe ratures,followed by a hydrothermal reaction to obtain Co3(OH)4Si2O5.Subsequently,CoSx and CoP-SiO2 were fabricated through sulfuration and phosphorization.The results in this work show that nanoreactors derived from metal-organic frameworks(MOFs) with a rational structure have broad development prospects.

关键词： Core-shell structure Metal oxide nanoparticles Co3(OH)4Si2O5 Nanoreactors Silicon shell

Visual detection of tropomyosin,a major shrimp allergenic protein using gold nanoparticles(AuNPs)-assisted colorimetric aptasensor

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Marine life Science & Technology 2021年第3期3卷 382-394页

作者： Tushar Ramesh Pavase Hong lin Maqsood Ahmed Soomro Hongwei Zheng xiaxia li Kexin Wang Zhenxing li Food Safety Laboratory Department of Food Science and EngineeringCollege of Food Science and EngineeringOcean University of ChinaQingdao266003China Fish Molecular Immunology Laboratory College of Marine Life SciencesOcean University of ChinaQingdao266003China

A gold nanoparticle-based label-free colorimetric assay was developed to detect the shrimp allergenic protein tropomyosin(TM),an important biomarker responsible for severe clinical reactivity to shellfish.In a gold nanoparticles(AuNPs)-tropomyosin-binding aptamer(TMBA)complex,the aptamer adsorbs onto the surface of AuNPs and dissociates in the presence of TM.In addition,AuNPs tend to aggregate in the presence of ionic salt,revealing a color change(i.e.,wine-red to purple/blue)with a shift in the maximum absorption peak from 520 nm.In the presence of specific binding TM,the aptamer folds into a tertiary structure where it more efficiently stabilizes AuNPs toward the salt-induced aggregation with a hypsochromic shift in the absorption spectra compared to the stabilized AuNPs by aptamer alone.Based on the aggregation and sensitive spectral transformation principle,the AuNPs-based colorimetric aptasensor was successfully applied to detect TM with a range of 10-200 nmol/L and a low detection limit of 40 nmol/L in water samples.The reliability,selectivity,and sensitivity of the aptasensor was then tested with food samples spiked with TM.The observed detection limit was as low as 70 nmol/L in shrimp,90 nmol/L in tofu,and 80 nmol/L in eggs,respectively.We anticipate the proposed AuNPs-based colorimetric aptasensor assay possesses a high potential for the easy and efficient visual colorimetric detection of TM.

关键词： Gold nanoparticles(AuNPs) Aptamer Aggregation Colorimetric assay Shellfish allergenic protein Shrimp tropomyosin(TM)

透过红木家具术语探析当代红木匠人的思维范式

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装饰 2024年第2期 109-113页

作者：张天星李霞霞隋文瀚五邑大学艺术与设计学院太原理工大学艺术学院中央美术学院

本文以红木家具术语为突破口,通过对用材、结构技法、纹饰图案与风格样式等术语进行总结,探析红木家具匠人的思维方式。本文的研究反映了红木家具行业的现状与问题,对红木家具行业的转型升级与发展具有参考价值。

关键词：红木家具术语红木匠人思维范式匠人认知

河西走廊不同产地‘美乐’葡萄品质及香气物质的差异分析

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园艺学报 2024年第5期51卷 1083-1098页

作者：马宗桓李玉梅韦霞霞李文芳陈佰鸿毛娟甘肃农业大学园艺学院兰州730070 广东省汕头市潮南区陇田镇公共服务中心广东汕头515135 甘肃省临夏州和政县农业农村局甘肃临夏731200

本研究旨在明确河西走廊不同产地‘美乐’葡萄果实品质及香气物质含量特征。对嘉峪关、张掖和武威3个产地连续3年的气候资料、土壤养分、果实品质及香气物质成分进行了分析。3个产地土壤普遍缺乏有机质,有机质含量均在4级(缺乏)以下,土... 详细信息

本研究旨在明确河西走廊不同产地‘美乐’葡萄果实品质及香气物质含量特征。对嘉峪关、张掖和武威3个产地连续3年的气候资料、土壤养分、果实品质及香气物质成分进行了分析。3个产地土壤普遍缺乏有机质,有机质含量均在4级(缺乏)以下,土壤pH在7.72~8.23之间,属于中性到碱性土壤;土壤表层大量元素氮、磷和钾的含量较为丰富,武威不同深度土壤中铁、铜和锰的含量较高。张掖产地4—9月的月有效积温和昼夜温差明显高于其他两个产地,而降水量以武威最多。3个产地果实品质中葡萄糖含量为张掖>嘉峪关>武威,蔗糖含量与之相反,武威产地酒石酸、柠檬酸、抗坏血酸含量高于其他两个产地。2018和2019年嘉峪关产地香气物质总含量高于其他两个产地。3个产地的环境因子温度、降水量等和果实糖酸含量显著相关,同时土壤中的钙、锰、铜和铁等矿质元素与果实糖酸含量也显著相关。由此可认为不同产地各类型香气物质的差异是环境因子和土壤营养共同作用的结果。

关键词：葡萄河西走廊品质香气物质

用于锂金属电池的聚合物固态电解质的研究进展

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化学学报 2024年第4期82卷 449-457页

作者：谷琪刘夏夏周鑫宇李江林秀婧马延文南京邮电大学信息材料与纳米技术研究院有机电子与信息显示国家重点实验室南京210023

聚合物固态电解质因其质轻安全、结构可设计以及优异的机械性能,成为高能量密度、高安全性全固态电池的潜在材料,逐渐成为研究的重点.但因其结晶度高、室温离子电导率低等缺陷也限制其进一步商业化.本综述梳理了近年来比较优越的聚合物... 详细信息

聚合物固态电解质因其质轻安全、结构可设计以及优异的机械性能,成为高能量密度、高安全性全固态电池的潜在材料,逐渐成为研究的重点.但因其结晶度高、室温离子电导率低等缺陷也限制其进一步商业化.本综述梳理了近年来比较优越的聚合物固态电解质种类,包括聚醚类聚合物、聚碳酸酯类聚合物、氟化聚合物、聚离子液体聚合物、单离子传导聚合物,围绕如何提升离子电导率,重点回顾了针对不同基质的结构设计和合成工艺方面的创新.最后阐述了聚合物固态电解质目前面临的基础研究和应用问题,以期为新型聚合物固态电解质的设计与制备提供新思路.

关键词：锂金属电池固态电解质聚合物电解质结构设计合成工艺

Dynamical analysis and optimal control for a delayed viral infection model

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International Journal of Biomathematics 2023年第4期16卷 21-44页

作者： Fei li Suxia Zhang xiaxia Xu School of Science Xi'an University of Technology Xi'an710048P.R.China

To describe the interaction between viral infection and immune response,we establish a mathematical model with intracellular delay and investigate an optimal control problem to examine the effect of antiviral therapy.Dynamic analysis of the proposed model for the stability of equilibria and Hopf bifurcation is conducted.Theoretical results reveal that the dynamical properties are determined by both the immune-inactivated reproduction number and the immune-activated reproduction number.With the aim of minimizing the infected cells and viral load with consideration for the treatment costs,the optimal solution is discussed analytically.Numerical simulations are performed to suggest the optimal therapeutic strategy and compare the model-predicted consequences.

关键词： Viral infection immune delay Hopf bifurcation optimal control