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Autophagy plays a pro-apoptotic role in arsenic trioxide-induced cell death of liver cancer

Journal of Integrative Medicine 2024年第3期22卷 295-302页

作者： Zhengting Deng Shufang Liang Guokai Huang Yuqian Wang xiaoyu tu Yani Zhang Shu Li Tao Liu Binbin Cheng Taizhou Hospital of Traditional Chinese Medicine Nanjing University of Chinese MedicineTaizhou 225300Jiangsu ProvinceChina Oncology Department of Traditional Chinese Medicine The First Affiliated Hospital of Naval Medical UniversityShanghai 200433China School of Traditional Chinese Medicine Nanjing University of Chinese MedicineNanjing 210023Jiangsu ProvinceChina Department of Gastroenterology Baoshan Hospital of Integrated Traditional Chinese and Western MedicineShanghai University of Traditional Chinese MedicineShanghai 201900China

Objective: The effects of arsenic trioxide(As2O3) on hepatocellular carcinoma have been documented widely. Autophagy plays dual roles in the survival and death of cancer cells. Therefore, we investigated the exact role of autophagy in As2O3-induced apoptosis in liver cancer cells.Methods: The viability of hepatoma cells was determined using the MTT assay with or without fetal bovine serum. The rate of apoptosis in liver cancer cells treated with As2O3was evaluated using flow cytometry, Hoechst 33258 staining, and tuNEL assays. The rate of autophagy among liver cancer cells treated with As2O3was detected using immunofluorescence, Western blot assay and transmission electron microscopy.Results: Upon treatment with As2O3, the viability of HepG2 and SMMC-7721 cells was decreased in a time-and dose-dependent manner. The apoptosis rates of both liver cancer cell lines increased with the concentration of As2O3, as shown by flow cytometry. Apoptosis in liver cancer cells treated with As2O3was also shown by the activation of the caspase cascade and the regulation of Bcl-2/Bax expression.Furthermore, As2O3treatment induced autophagy in liver cancer cells;this finding was supported by Western blot, immunofluorescence of LC3-II and beclin 1, and transmission electron microscopy. In liver cancer cells, As2O3inhibited the phosphoinositide 3-kinase/protein kinase B/mammalian target of rapamycin(PI3K/AKT/mTOR) signal pathway that plays a vital role in both apoptosis and autophagy. The PI3K activator SC-79 partially reversed As2O3-induced autophagy and apoptosis. Furthermore, inhibiting autophagy with 3-methyladenine partially reversed the negative effects of As2O3on cell viability.Serum starvation increased autophagy and amplified the effect of As2O3on cell death.Conclusion: As2O3induces apoptosis and autophagy in liver cancer cells. Autophagy induced by As2O3may have a proapoptotic effect that helps to reduce the viability of liver cancer cells. This study provides novel insights into the effects of As2O3against liver cancer.

关键词： Apoptosis Arsenic trioxide Autophagy Primary liver cancer

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A combinatorial indexing strategy for low-cost epigenomic profiling of plant single cells

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Plant Communications 2022年第4期3卷 72-82页

作者： xiaoyu tu Alexandre P.Marand Robert J.Schmitz Silin Zhong Joint Center for Single Cell Biology School of Agriculture and BiologyShanghai Jiao Tong UniversityShanghai 200240China Shanghai Collaborative Innovation Center of Agri-Seeds School of Agriculture and BiologyShanghai Jiao Tong UniversityShanghai 200240China Department of Genetics University of GeorgiaAthensGA 30602USA State Key Laboratory of Agrobiotechnology School of Life SciencesThe Chinese University of Hong KongHong KongChina

Understanding how cis-regulatory elements facilitate gene expression is a key question in biology.Recent advances in single-cell genomics have led to the discovery of cell-specific chromatin landscapes that underlie transcription programs in animal models.However,the high equipment and reagent costs of commercial systems limit their applications for many laboratories.In this study,we developed a combinatorial index and dual PCR barcode strategy to profile the Arabidopsis thaliana root single-cell epigenome without any specialized equipment.We generated chromatin accessibility profiles for 13576 root nuclei with an average of 12784 unique Tn5 integrations per cell.Integration of the single-cell assay for transposaseaccessible chromatin sequencing and RNA sequencing data sets enabled the identification of 24 cell clusters with unique transcription,chromatin,and cis-regulatory signatures.Comparison with single-cell data generated using the commercial microfluidic platform from 10X Genomics revealed that this low-cost combinatorial index method is capable of unbiased identification of cell-type-specific chromatin accessibility.We anticipate that,by removing cost,instrumentation,and other technical obstacles,this method will be a valuable tool for routine investigation of single-cell epigenomes and provide new insights into plant growth and development and plant interactions with the environment.

关键词： combinatorial indexing single-cell ATAC-seq epigenome

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论全面深化改革背景下的国家能力建设

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理论视野 2024年第5期291卷 52-57页

作者：涂小雨河南省委党校决策咨询部

国家能力指国家集体行动的能力,即为保持国家生存与可持续发展的总体能力,包括但不限于综合国力、国际竞争力、国际影响力、国家治理等的综合性命题,贯通了政治、经济、文化、社会、生态、安全与发展态势,涵盖了政治学、社会学、经济学... 详细信息

国家能力指国家集体行动的能力,即为保持国家生存与可持续发展的总体能力,包括但不限于综合国力、国际竞争力、国际影响力、国家治理等的综合性命题,贯通了政治、经济、文化、社会、生态、安全与发展态势,涵盖了政治学、社会学、经济学等学科体系,是对国家意志、国家精神、国家意识、国民意识、国民精神的集中表达和整体性把握。从量与质的二重规定性角度看,国家能力可分为静态的国家能力和动态的国家能力。国家能力建设是一个渐进和逐渐积累的过程,具有继承与创新的双重意义,并日益超脱出经济成就的范畴,在国家治理制度体系上表现为形成了一系列符合中国国情的根本制度、基本制度、重要制度。

关键词：全面深化改革国家能力建设规定性基本特征

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Tissue-specific Hi-C analyses of rice, foxtail millet and maize suggest non-canonical function of plant chromatin domains

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Journal of Integrative Plant Biology 2020年第2期62卷 201-217页

作者： Pengfei Dong xiaoyu tu Haoxuan Li Jianhua Zhang Donald Grierson Pinghua Li Silin Zhong State Key Laboratory of Agrobiotechnology School of Life SciencesThe Chinese University of Hong KongHong KongChina Zhejiang Provincial Key Laboratory of Horticultural Plant Integrative Biology Zhejiang UniversityHangzhou 310058China State Key Laboratory of Crop Biology College of AgronomyShandong Agricultural UniversityTai’an 271018China

Chromatins are not randomly packaged in the nucleus and their organization plays important roles in transcription regulation,which is best studied in the mammalian models.Using in situ Hi-C,we have compared the 3D chromatin architectures of rice mesophyll and endosperm,foxtail millet bundle sheath and mesophyll,and maize bundle sheath,mesophyll and endosperm tissues.We found that their global A/B compartment partitions are stable across tissues,while local A/B compartment has tissue-specific dynamic associated with differential gene expression.Plant domains are largely stable across tissues,while new domain border formations are often associated with transcriptional activation in the region.Genes inside plant domains are not conserved across species,and lack significant co-expression behavior unlike those in mammalian TADs.Although we only observed chromatin loops between gene islands in the large genomes,the maize loop gene pairs’syntenic orthologs have shorter physical distances in small genome monocots,suggesting that loops instead of domains might have conserved biological function.Our study showed that plants’chromatin features might not have conserved biological functions as the mammalian ones.

关键词： plant chromatin domains Tissue-specific Hi-C analyses rice

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习近平总书记关于做好新时代党的统一战线工作的重要思想的原创性贡献和时代价值

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广州社会主义学院学报 2024年第2期 18-23页

作者：涂小雨中共河南省委党校决策咨询部河南郑州451000

党的十八大以来,习近平总书记对统一战线工作发表了一系列重要讲话、作出了一系列重要指示批示,形成了符合中国国情、顺应新时代特点和要求的关于加强和改进统一战线工作的重要思想。习近平总书记关于做好新时代党的统一战线工作的重要... 详细信息

党的十八大以来,习近平总书记对统一战线工作发表了一系列重要讲话、作出了一系列重要指示批示,形成了符合中国国情、顺应新时代特点和要求的关于加强和改进统一战线工作的重要思想。习近平总书记关于做好新时代党的统一战线工作的重要思想是习近平新时代中国特色社会主义思想的重要内容,科学回答了统一战线一系列重大理论和实践问题,深化了我们党对共产党执政规律、社会主义建设规律和人类社会发展规律的认识,丰富和发展了马克思主义统一战线理论。

关键词：新时代统一战线重叠共识

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3D Chromatin Architecture of Large Plant Genomes Determined by Local A/B Compartments

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Molecular Plant 2017年第12期10卷 1497-1509页

作者： Pengfei Dong xiaoyu tu Po-Yu Chu Peitao Lu Ning Zhu Donald Grierson Baijuan Du Pinghua Li Silin Zhong State Key Laboratory of Crop Biology College of Agronomy Shandong Agricultural University Tai'an Shandong China State Key Laboratory of Agrobiotechnology School of Life Sciences The Chinese University of Hong Kong Hong Kong China Zhejiang Provincial Key Laboratory of Horticultural Plant Integrative Biology Zhejiang University Hangzhou Zhejiang China These authors contributed equally to this article.

The spatial organization of the genome plays an important role in the regulation of gene expression. How- ever, the core structural features of animal genomes, such as topologically associated domains （TADs） and chromatin loops, are not prominent in the extremely compact Arabidopsis genome. In this study, we examine the chromatin architecture, as well as their DNA methylation, histone modifications, accessible chromatin, and gene expression, of maize, tomato, sorghum, foxtail millet, and rice with genome sizes ranging from 0.4 to 2.4 Gb. We found that these plant genomes can be divided into mammalian-like A/B compartments. At higher resolution, the chromosomes of these plants can be further partitioned to local AJB compartments that reflect their euchromatin, heterochromatin, and polycomb status. Chromatins in all these plants are organized into domains that are not conserved across species. They show similarity to the Drosophila compartment domains, and are clustered into active, polycomb, repressive, and interme- diate types based on their transcriptional activities and epigenetic signatures, with domain border overlaps with the local A/B compartment junctions. In the large maize and tomato genomes, we observed extensive chromatin loops. However, unlike the mammalian chromatin loops that are enriched at the TAD border, plant chromatin loops are often formed between gene islands outside the repressive domains and are closely associated with active compartments. Our study indicates that plants have complex and unique 3D chromatin architectures, which require further study to elucidate their biological functions.

关键词： Hi-C chromatin loop compartment domain local compartment

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党的十八大以来金融反腐工作的现实形势、实践路径与基本经验

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湖北理工学院学报（人文社会科学版） 2024年第3期41卷 16-26页

作者：李斌雄涂笑宇武汉大学马克思主义学院湖北武汉430072 武汉大学党内法规研究中心湖北武汉430072

新时代我国金融业的快速发展增加了金融腐败的可能性,资本在金融领域的密集和科技的创新迭代使金融腐败的隐蔽性、诱惑性与风险性大大增加。与此同时,金融机构在制度、体制与机制上的不完善,加之金融监管漏洞等问题的存在,金融反腐形势... 详细信息

新时代我国金融业的快速发展增加了金融腐败的可能性,资本在金融领域的密集和科技的创新迭代使金融腐败的隐蔽性、诱惑性与风险性大大增加。与此同时,金融机构在制度、体制与机制上的不完善,加之金融监管漏洞等问题的存在,金融反腐形势严峻。党的十八大以来,党中央坚持标本兼治,一体推进金融领域“三不腐”,金融反腐取得显著成效,金融反腐经验日益丰富。十八大以来金融反腐工作的基本经验主要包括四个方面,即坚持党对金融工作的集中统一领导、坚持完善金融监管、坚持强化金融监督、坚持金融反腐工作的系统施治。

关键词：十八大中国共产党金融反腐反腐经验

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Back-contact configuration energizes perovskite photovoltaic modules

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Nano Research Energy 2024年第2期3卷 7-11页

作者： xiaoyu Yang Yongguang tu Fengjun Ye Zheng Bao State Key Laboratory for Artificial Microstructure and Mesoscopic Physics School of PhysicsPeking UniversityBeijing 100871China Frontiers Science Center for Flexible Electronics(FSCFE) Xi’an Institute of Flexible Electronics(IFE)&Xi’an Institute of Biomedical Materials and Engineering(IBME)Northwestern Polytechnical University Xi’anShaanxi 710072China Beijing Solarverse Optoelectronic Technology Co. Ltd.Beijing 100176China

In this viewpoint,recent hot topics in the photovoltaic community,interdigitated back contact(IBC)cells,are systematically reviewed from the view of device configuration.Two categories of IBC designs on the most popular perovskite solar cells(PSCs)were discussed,and a planar back-contact perovskite module was first proposed.The device configuration,fabrication methods,working mechanism,optimization strategies,and future development directions of this novel PSC module were put forward to show its superiorities in the module performance,processing difficulty,and extensible functionality among present perovskite modules,presenting promising potential to improve the competitiveness of perovskite technology in the photovoltaic market.

关键词： perovskite solar cell back-contact photovoltaic technology device configuration

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Granulomatosis with polyangiitis presented as multiple cutaneous abscesses after hysterectomy

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Chinese Medical Journal 2024年第6期137卷 755-756页

作者： Xiaoting Song Mengrui Li Yinggai Song Ping tu Hang Li Yang Wang Rong Xu Min Chen Xiaojuan Yu Suxia Wang Fude Zhou Minghui Zhao Ruoyu Li xiaoyu Jia Xiaowen Wang Department of Dermatology and Venereology Peking University First HospitalBeijing 100034China Beijing Key Laboratory of Molecular Diagnosis on Dermatoses Beijing 100034China National Clinical Research Center for Skin and Immune Diseases Beijing 100034China Renal Division Department of MedicinePeking University First HospitalBeijing 100034China Institute of Nephrology Peking UniversityBeijing 100034China Key Laboratory of Renal Disease Ministry of Health of ChinaBeijing 100034China Research Units of Diagnosis and Treatment of Immune-mediated Kidney Diseases Chinese Academy of Medical SciencesBeijing 100034China

To the Editor:A 62-year-old woman presented to the dermatology department with a 5-month history of multiple painless cutaneous abscesses and ulcers[Figure 1A],occurring 1 month after a transvaginal hysterectomy due to uterine prolapse.Her general condition was good before and after the surgery,with no abnormal physical signs apart from metroptosis.She reported no fever,night sweat,cough,or abdominal pain,but 10 kg weight-loss.She has no history of diabetes,autoimmune diseases,or chronic infectious diseases.There was no similar illness in her family.

关键词： hysterectomy pain surgery

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习近平关于“国之大者”的重要论述: 背景、内涵与时代价值

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黄河科技学院学报 2023年第7期25卷 26-33页

作者：涂小雨中共河南省委党校决策咨询部河南郑州450018

2020年4月以来,习近平总书记从党和国家事业发展全局出发,立足中华民族伟大复兴战略全局和世界百年未有之大变局,统筹国内国际两个大局,多次发表关于“国之大者”的重要论述,对新时代党员领导干部胸怀“国之大者”的使命担当提出了明确... 详细信息

2020年4月以来,习近平总书记从党和国家事业发展全局出发,立足中华民族伟大复兴战略全局和世界百年未有之大变局,统筹国内国际两个大局,多次发表关于“国之大者”的重要论述,对新时代党员领导干部胸怀“国之大者”的使命担当提出了明确要求。这充分彰显了习近平总书记高瞻远瞩的战略眼光、居安思危的政治清醒、兴党强国的使命担当,对广大党员领导干部在新时代新征程中践行初心使命、增强敢于担当善于作为的政治自觉和行动自觉具有极为重要的指导意义。习近平总书记关于“国之大者”的重要论述构成了习近平新时代中国特色社会主义思想的重要组成部分,是推进国家治理体系和治理能力现代化的重要指针,是巩固全党团结统一的重要遵循。

关键词：国之大者深刻内涵时代价值

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