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An ex vivo assay for screening glucocorticoid signaling disruption based on glucocorticoid-response gene transcription in Xenopus tails

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Journal of Environmental Sciences 2018年第4期30卷 104-112页

作者： xiaoying chen Yuanyuan Li Min Zhu Zhanfen Qin State Key Laboratory of Environmental Chemistry and Ecotoxicology Research Center for Eco-Environmental Sciences Chinese Academy of Sciences Beijing 100085 China University of Chinese Academy of Sciences Beijing 100049 China School of Environmental Sciences and Engineering Nanjing Tech University Nanjing 211816 China

There is a pressing need for developing in vivo or ex vivo assays to screen the glucocorticoid（GC） signaling disruption of chemicals. Thus, we aimed to establish an ex vivo assay for screening GC signaling disruption based on the GC-response gene transcription in Xenopus laevis tails cultured ex vivo. Firstly, we investigated effects of corticosterone（CORT, a main GC in frogs） on GC-response gene expression, and determined the six genes as molecular endpoints for assaying the GC signaling disruption. CORT in the range of 1.56–400 nmol/L was found to up-regulate transcription of the six GC-response genes, exhibiting comparable or higher sensitivity than previously reported assays. To validate this ex vivo assay, then, we examined effects of dexamethasone（a known GC signaling agonist） on GC-response gene expression. Dexamethasone displayed an agonistic action in a concentration-dependent manner, further demonstrating the efficiency of the established assay. Finally, we applied the ex vivo assay to evaluate the GC signaling disruption of bisphenol A（BPA）. In accordance with previous reports, we found a concentration-dependent agonistic activity of BPA,showing that the established assay is effective for detecting the GC signaling disrupting activity of environmental chemicals. Correspondingly, the GC signaling agonistic actions of CORT and BPA in ex vivo tails accorded with the observations in vivo, indicating that the ex vivo assay is able to detect the actions of chemicals in vivo. Overall, we established an ex vivo assay that can effectively screen GC signaling disruption of environmental chemicals.

关键词： Glucocorticoid signaling Glucocorticoid-response gene Xenopus laevis tail Dexamethasone Bisphenol A(BPA)

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Characteristics of Heavy Minerals and Quantitative Provenance Identification of Sediments from the Muddy Area Outside the Oujiang Estuary Since 5.8 kyr

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Journal of Ocean University of China 2018年第6期17卷 1325-1335页

作者： SONG Hongying LIU Jinqing YIN Ping ZHANG Yong chen xiaoying Center for Isotope Geochemistry and Geochronology Qingdao National Laboratory for Marine Science and Technology Key Laboratory of Marine Environmental Geology Ministry of Natural Resources Qingdao Institute of Marine Geology Laboratory for Marine Geology Qingdao National Laboratory for Marine Science and Technology College of Earth Science and Engineering Shandong University of Science and Technology

The heavy mineral compositions of the sediments in core D04 with a length of 20.2 m from the muddy area outside the Oujiang Estuary along the coast of Zhejiang-Fujian Provinces have been analyzed. Core D04 can be divided into three units: DU1(0–7.4 m), DU2(7.4–11.4 m) and DU3(11.4–20.2 m). The results showed that the heavy minerals are authigenic pyrite(65.6%)– hornblende(16.0%) – epidote(4.6%) assemblages. The core sediments are mainly composed of clayey silt, which belongs to the clinoform deposit formed since 5.8 kyr, indicating a weak hydrodynamic environment. The deposition rates changed in the trend of high-low-high upward. Quantitative analysis shows that the core sediments were mainly from the suspended sediments of the Changjiang(Yangtze) River, accounting for 79.2%, with only 10.0% from Oujiang, and 10.8% from other provenance. The Oujiang-derived sediments were gradually increased from the bottom unit DU3(3.1%) to the top unit DU1(17.8%), but the Changjiang-derived sediments were gradually decreased. The source changes are closely related to the development of Yangtze River Delta and Wenzhou Bay, climate changes and human activities. During the period of 2.1–3.7 kyr, the sediment supply was greatly reduced due to the poor reclamation capacity of the river basins, resulting in a low deposition rate. Since 2 kyr, the enhancement of human activities in the Yangtze River Basin and the Oujiang River Basin led to more soils to be eroded and transported to the study area. Due to the short distance of the site of core D04 from the Oujiang River mouth, the study area received more Oujiang-derived sediments when upper unit(DU1) deposited than the lower units(DU2 and DU3).

关键词： mud deposit Oujiang Estuary mid-Holocene heavy mineral quantitative identification

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Composition, Source and Environmental Indication of Clay Minerals in Sediments from Mud Deposits in he Southern Weihai Offshore, Northwestern Shelf of the South Yellow Sea, China

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Journal of Ocean University of China 2022年第5期21卷 1161-1173页

作者： LIU Jinqing LIU Yilin YIN Ping GAO Fei CAO Ke chen xiaoying College of Earth Science and Engineering Shandong University of Science and TechnologyQingdao 266590China Laboratory for Marine Mineral Resources Qingdao National Laboratory for Marine Science and TechnologyQingdao 266071China Qingdao Institute of Marine Geology China Geological SurveyQingdao 266071China

Studying the sedimentary characteristics of the muddy area along the Yellow Sea shelf is of great significance for deep-ening the understanding of the climate and environment evolution since the last glacial ***,a small muddy area developed in the southern Weihai offshore has attracted a lot of *** on high-resolution grain size,clay mineral,AMS14C,and OSL data of sediments from the core WHZK01 in the muddy area,we acquired a new understanding of sedimentary characteristics,sources and paleoclimatic environment during the last 13 *** results show that the core WHZK01 sediments were mainly from the Yellow River and local rivers along the ***,the sources varied for different deposition *** riverbed(before 13 kyr BP)and fluvial plain deposits(13-10 kyr BP)were mainly from local river *** very thin littoral deposits from 10 kyr BP to 8 kyr BP also mainly originate from coastal river inputs,while the littoral-neritic deposits since 8 kyr BP were dominated by the Yellow River materials and partly supplied by the coastal rivers and the island *** addition,five events related with the strong East Asian winter monsoon(EAWM)during the intervals of 13.0-11.0 kyr BP,10.0-8.2 kyr BP,7.0-5.0 kyr BP,3.5-2.5 kyr BP,and 1.2-0.5 kyr BP were *** events related with the enhancement of the EAWM since the middle Holocene have strengthened the transport capacity of the coastal currents of the Shandong ***,more Yellow River-derived distal sediments were deposited to the southern Weihai offshore and formed wedge-shaped muddy *** short,the ratio of smectite/illite could be used as an effective EAWM indicator since 13 kyr BP on the northwestern shelf of the South Yellow Sea.

关键词： clay minerals sediment provenance East Asian winter monsoon mud deposits southern Weihai offshore South Yellow Sea

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Permanence and Global Attractivity of a Discrete Nicholson's Blowflies Model with Delay

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Journal of Mathematical Research with Applications 2017年第2期37卷 233-241页

作者： xiaoying chen Chunling SHI Department of Computer Engineering Fuzhou University Zhicheng College

In this paper, we consider a discrete Nicholson's blowflies model with delay. By constructing suitable Lyapunov functional, a sufficient condition for the permanence and global attractivity of the system is obtained. ... 详细信息

关键词： discrete Nicholson’s blowflies model delay permanence global attractivity

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Effects of dietary lipid-to-carbohydrate ratio on growth and carbohydrate metabolism in juvenile cobia(Rachycentron canadum)

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Animal Nutrition 2020年第1期6卷 80-84页

作者： Hongxia Zhao Junming Cao xiaoying chen Guoxia Wang Junru Hu Bing chen Institute of Animal Science Guangdong Academy of Agricultural SciencesGuangzhou 510640China Key Laboratory of Animal Nutrition and Feed Science in South China of Ministry of Agriculture Guangzhou 510640China Guangdong Key Laboratory of Animal Breeding and Nutrition Guangzhou 510640China

This experiment was conducted to investigate the effects of dietary lipid-to-carbohydrate ratio on growth and carbohydrate metabolism in juvenile cobia(Rachycentron canadum).Six iso nitrogenous diets were prepared to vary in lipid-to-carbohydrate ratio(g/g)as follows:Dl,2.26;D2,1.31;D3,0.78;D4,0.47;D5,0.34;and D6,*** were fed to satiety for 8 *** weight gain and protein efficiency ratio in Dl group were significantly lower than those in other groups(P<0.05),accompanied by a lower level of feed conversion ratio(P<0.05).Protein retention efficiency in D4 and D6 and whole body protein in D4 and D5 were significantly higher than those in D1 group(P<0.05).Survival rate in D4 group was the highest among all groups and was significantly higher than that in Dl,D2 and D5(P<0.05).In terms of serum triglyceride,D1 and D2 were significantly higher than D6(P<0.05).Hepatosomatic index in D3 and D4 was significantly lower than that in D1(P<0.05).Fructose-1,6-diphosphatase in D4 was significantly higher than that in D1 and D3(P<0.05).Phosphofructokinase in D3 and D4 and malic enzyme in D4 and D5 were significantly higher than those in other groups(P<0.05).Results indicate that cobia utilizes carbohydrates as energy source more efficiently than it utilizes *** optimal lipid-to-carbohydrate ratio in juvenile cobia diets is 0.47.

关键词： Rachycentron canadum Lipid-to-carbohydrate ratio Growth Carbohydrate metabolism

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SRWD1, a novel target gene of DELLA and WRKY proteins, participates in the development and immune response of rice (Oryza sativa L.)

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Science Bulletin 2017年第24期62卷 1639-1648页

作者： Chuanmiao Xie Ge Zhang Yuman Zhang Xiaoguang Song Hongyan Guo xiaoying chen Rongxiang Fang State Key Laboratory of Plant Genomics Institute of Microbiology Chinese Academy of Sciences Beijing 100101 China National Center for Plant Gene Research (Beijing) Beijing 100101 China University of Chinese Academy of Sciences Beijing 100049 China State Key Laboratory of Plant Genomics and National Center for Plant Gene Research (Beijing) Institute of Genetics and Developmental Biology Chinese Academy of Sciences Beijing 100101. China

SRWD1, a member of the WD40 protein subfamily, is induced by salt stress in rice and its homolog in barley can bind to GAMYB, implying that SRWD1 might be involved in plant defense against environmental stress and gibberellic acid(GA) signalings. In this study, we focused on the biological functions and regulation mechanisms of SRWD1 in rice. The results showed that SRWD1 expression was repressed by GA and induced by abscisic acid(ABA). Two WRKY-family transcription factors, Os WRKY45 and Os WRKY72, were found to regulate SRWD1 expression by directly binding to the W-box region in its promoter. Transient co-expression and yeast two-hybrid analyses showed that a DELLA protein strengthened the activation of Os WRKY45 and partly relieved the suppression of Os WRKY72 by binding to ***, both SRWD1-overexpressing transgenic plants and SRWD1-knockout mutants showed dwarf phenotypes and resistance to Xanthomonas oryzae.

关键词： Phytohormone Gibberellin(GA) Abscisic acid(ABA) Signal transduction DELLA protein WRKY family protein

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Small G Protein as a Novel Component of the Rice Bmssinosteroid Signal Transduction

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Molecular Plant 2016年第9期9卷 1260-1271页

作者： Ge Zhang Xiaoguang Song Hongyan Guo Yao Wu xiaoying chen Rongxiang Fang State Key Laboratory of Plant Genomics Institute of Microbiology Chinese Academy of Sciences Beijing 100101 China National Center for Plant Gene Research (Beijing) Beijing 100101 China University of Chinese Academy of Sciences Beijing 100049 China These authors contributed equally to this article. Present address: State Key Laboratory of Plant Genomics and NationaT Center for Plant Gene Research (Beijing) Institute of Genetics and Developmental BiologyChinese Academy of Sciences Beijing 100101 China

Brassinosteroids （BRs） are a class of steroid hormones that are essential for plant growth and development. The BR signal transduction pathway in the dicot model plantArabidopsis is well established, but the components connecting the BR signaling steps in rice have not been fully explored. For example, how the BR signaling is fine-tuned in rice, especially at the BR receptor level, is largely unknown. Here we show that OsPRA2, a rice small G protein, plays a repressive role in the BR signaling pathway. Lamina inclination, coleoptile elongation, and root inhibition assays indicated that rice plants with suppressed expression of OsPRA2 were more sensitive to exogenously applied brassinolide than the wild-type plants. Conversety, rice overexpressing OsPRA2 was less sensitive to exogenous brassinolide. Further study uncovered that OsPRA2 inhibited the dephosphorylation of, and thus inactivated the transcription factor BRASSINAZOLE- RESISTANT 1 （OsBZR1）. More importantly, OsPRA2 was found to co-localize with and directly bind to rice BR receptor BRASSlNOSTEROID-INSENSITIVE 1 （OsBRI1） at the plasma membrane. Additionally, the in vitro assays showed that OsPRA2 inhibits its autophosphorylation. This OsPRA2-OsBRI1 interaction led to the dissociation of OsBRI1 from its co-receptor OsBAK1, and abolished OsBRIl-mediated phosphorylation of OsBAK1. Together, these results reveal a possible working mechanism of OsPRA2 as a novel negative regu- lator on OsBRI1 and OsBZR1 and extend the knowledge about the regulatory mechanism of rice BR signaling.

关键词： brassinosteroid small G protein plant hormone signal transduction

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A comparative study on the tolerance of tilapia(Oreochromis niloticus)to high carbohydrate and high lipid diets

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Animal Nutrition 2023年第2期13卷 160-172页

作者： Lijun Ning Haotian Zhang xiaoying chen Jia Zhen Sen chen Junfeng Guang Chao Xu Yuanyou Li University Joint Laboratory of Guangdong Province Hong Kong and Macao Region on Marine Bioresource Conservation and ExploitationCollege of Marine SciencesSouth China Agricultural University&Maoming BranchGuangdong Laboratory for Lingnan Modern AgricultureGuangzhou 510642GuangdongChina Laboratory of Aquatic Sciences Key Laboratory of Animal Nutrition and Feed Science in South China of Ministry of Agriculture and Rural AffairsGuangdong Key Laboratory of Animal Breeding and NutritionInstitute of Animal ScienceGuangdong Academy of Agricultural SciencesGuangzhou 510640China

A 12-wk trial was conducted to compare the tolerance of tilapia to high carbohydrate and high lipid *** isonitrogenous and isoenergetic diets,whose carbohydrate and lipid levels were the following:35.0%and 8%(control),44.2%and 4%(D1,high carbohydrate),and 25.8%and 12%(D2,high lipid),*** hundred tilapias(27±0.11 g)were fed the diets for 10 wk(4 replicates per group);72 fish from the D1 group were continually fed the D1(D1D1)and 72 fish from the D2 were continually fed the D2(D2D2)diet for 2 wk(3 replicates each group)to evaluate the tilapia's capacity to tolerate high carbohydrate and high lipid diets,*** 36 fish from D1 group were continually fed D2(D1D2)for comparison with D1D1 and D2D2 *** phase 1,hepatosomatic index,liver triglycerides(TG),glucose tolerance(GT)and crude protein in the whole body in D1 group were higher than those in D2 group(P<0.05).During phase 2,D1D1 group had lower feed intake and weight gain,as well as lower serum total protein and albumin than that of D2D2 group(P<0.05),while its liver glycogen was significantly higher than that in D1D2 and D2D2 groups(P<0.05).Moreover,serum glucose and GT were higher in D1D1 and D1D2 groups than those in D2D2 group(P<0.05).By contrast,D2D2 group had significantly higher intraperitoneal fat,subcutaneous adipose tissue(SCAT)and liver TG than those in D1D1 group(P<0.05).The mRNA expression of brain npy,hepatic nrf2,gst1 and hepatic transcriptomic data showed that immune-related genes(gama,mrc2,mhc2 and cd163),were downregulated in D1D1 group compared to D2D2 and D1D2 *** together:1)tilapia have higher tolerance to a high lipid diet than high carbohydrate diet;2)despite retention of glucose tolerance,the continuous feeding of D1 diet impaired tilapia's appetite,weight gain rate and host immune response;3)specific distri-bution of fat in intraperitoneal regions,SCAT and liver may be a risk-avoidance strategy in tilapia in response to a continuous D2 diet.

关键词： Keywords:Carbohydrate and lipid Tilapia Glucose tolerance Subcutaneous adipose tissue

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Texture Feature Extraction Method for Ground Nephogram Based on Contourlet and the Power Spectrum Analysis Algorithm

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Computers, Materials & Continua 2019年第8期61卷 861-875页

作者： xiaoying chen Shijun Zhao Xiaolei Wang Xuejin Sun Jing Feng Nan Ye College of Mechanical&Electrical Engineering Sanjiang UniversityNanjingChina Institute of Meteorology and Oceanography National University of Defense TechnologyNanjingChina Department of Civil Engineering Monash UniersityMelbourneAustralia

It is important to extract texture feature from the ground-base cloud image for cloud type automatic *** this paper,a new method is presented to capture the contour edge,texture and geometric structure of cloud images by using Contourlet and the power spectrum analysis *** abundant texture information is *** images can be obtained a multiscale and multidirection *** coefficient matrix from Contourlet transform of ground nephogram is *** energy,mean and variance characteristics calculated from coefficient matrix are composed of the feature *** frequency information of the data series from the feature vector values is obtained by the power spectrum *** Support Vector Machines(SVM)classifier is used to classify according to the frequency information of the trend graph of data *** is shown that altocumulus and stratus with different texture frequencies can be effectively recognized and further subdivided the types of clouds.

关键词： Ground nephogram super-wavelet Contourlet the power spectrum

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Designing specific bacterial 16S primers to sequence and quantitate plant endo-bacteriome

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Science China(Life Sciences) 2022年第5期65卷 1000-1013页

作者： Liying chen Mengting Zhang Da Liu Hongbo Sun Jianxiang Wu Yan Huo xiaoying chen Rongxiang Fang Lili Zhang State Key Laboratory of Rice Biology Institute of BiotechnologyZhejiang UniversityHangzhou 310058China State Key Laboratory of Plant Genomics Institute of MicrobiologyChinese Academy of SciencesBeijing 100101China University of Chinese Academy of Sciences Beijing 100049China National Plant Gene Research Center Beijing 100101China

Plant endophytic bacteria colonize the internal tissues of plants and interact with plants *** past two decades have witnessed the increasing application of next-generation 16S r RNA gene sequencing in the investigation of bacterial ***,deciphering plant endo-bacterial communities by this method is difficult because of the co-amplification of massive plant organellar DNAs with bacterial ***,we designed polymerase chain reaction(PCR) primer sets,including799F/1107R,322F/796R,and 322F-Dr/796Rs(primer pair 322F/796R with a penultimate-base substitution in 322F),that can specifically amplify bacterial 16S from plant total *** computationally and experimentally evaluated the specificity,coverage,and accuracy of the newly designed primer *** 799F/1107R and 322F-Dr/796Rs produced plant DNA-free 16S amplicon libraries or reduced plant DNA contamination to lower than 5% for the plant materials with extremely-low-abundance bacterial *** primer set 322F-A/796R was used through absolute quantitative PCR to quantitate the population size of rice leaf or root endo-bacteriome,which revealed 10^(6)–10^(7) and 10^(9)–10^(10) bacteria per gram fresh weight,*** 16S primer sets and amplification methods enable the simple and inexpensive next-generation sequencing and quantification of plant endo-bacteriome,which will significantly advance studies on the plant-related microbiome.

关键词： plant endo-bacteriome plant DNA-free amplification 16S rRNA gene next-generation sequencing absolute quantification

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