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高等医学院校实验室危化品管理的现状及对策

化工管理 2024年第3期 101-104页

作者：赵小亚常继硕余细勇广州医科大学药学院广东广州510182

高等医学院校实验室是生物医药研究的重要场所,危化品在实验室中使用较为广泛。危化品的种类繁多且具有性质复杂的特性,导致管理难度大及存在较多安全隐患。管理好危化品、防范事故风险是高校实验室安全管理的重中之重。文章分析了高等... 详细信息

高等医学院校实验室是生物医药研究的重要场所,危化品在实验室中使用较为广泛。危化品的种类繁多且具有性质复杂的特性,导致管理难度大及存在较多安全隐患。管理好危化品、防范事故风险是高校实验室安全管理的重中之重。文章分析了高等医学院校实验室危化品的管理现状并提出对策,以期改善实验室危化品的管理,保障师生安全。

关键词：实验室危化品安全管理

表达外源基因SPAM1重组CAV-2溶瘤病毒的构建与拯救

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畜牧兽医学报 2024年第3期55卷 1228-1237页

作者：高龙常心怡李程赵晓亚李汶洁范浩谦马静云华南农业大学动物科学学院广州510642

旨在研究犬腺病毒-2型(canine adenovirus type 2,CAV-2)作为溶瘤病毒的潜力并进一步探索精子黏附因子(SPAM1)协同CAV-2重塑肿瘤微环境在肿瘤免疫治疗中的应用价值。本研究以P15A-CAV-2反向遗传操作平台为基础,使用RED/ET同源重组系统... 详细信息

旨在研究犬腺病毒-2型(canine adenovirus type 2,CAV-2)作为溶瘤病毒的潜力并进一步探索精子黏附因子(SPAM1)协同CAV-2重塑肿瘤微环境在肿瘤免疫治疗中的应用价值。本研究以P15A-CAV-2反向遗传操作平台为基础,使用RED/ET同源重组系统构建携带SPAM1外源基因的中间载体,使用中间载体将CAV-2骨架载体E3区域缺失并替换SPAM1外源基因表达盒。再利用合成引物敲除kmccdB反向筛选表达盒构建P15A-CAV-2-mCMV-SPAM1-SV40 polyA感染性克隆质粒并在MDCK-E1A细胞系中拯救重组溶瘤病毒,对重组病毒体外溶瘤效应进行验证。根据测序与酶切验证结果证明,本研究成功构建并拯救出稳定表达外源基因SPAM1的重组溶瘤病毒1株;IFA试验证明,重组毒株能够高水平稳定表达外源基因且外源蛋白具有可弥散分布于细胞间的特点。缺失E3区域表达外源基因SPAM1的重组溶瘤病毒通过光镜观察和CCK8检测发现具有对犬癌细胞系A72强烈的杀伤效应。综上,本研究成功构建1株具有良好溶瘤效应的重组CAV-2,为后续应用于宠物肿瘤治疗奠定了基础。

关键词：溶瘤病毒精子黏附因子病毒反向遗传操作犬腺病毒-2

Cascade Synthesis of Luminescent Difluoroboron Diketonate Compounds for Room-Temperature Organic Afterglow Materials

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Chinese Journal of Chemistry 2022年第21期40卷 2507-2515页

作者： Junbo Li Xuepu Wang xiaoya zhao Xuefeng Chen Shuhui Ding Minjian Wu Kaka Zhang College of Chemistry and Materials Science Sichuan Normal UniversityChengduSichuan 610068China Key Laboratory of Synthetic and Self-Assembly Chemistry for Organic Functional Molecules Shanghai Institute of Organic ChemistryUniversity of Chinese Academy of SciencesChinese Academy of Sciences345 Lingling RoadShanghai 200032China

Difluoroboronβ-diketonate(BF2bdk)compounds represent an important class of luminescent materials,whereas their synthesis requires multiple steps,which restrict their application in diverse *** we report a cascade reaction to prepare BF2bdk from aromatic ketones,carboxylic acids,trifluoroacetic anhydride and boron trifluoride diethyl *** cascade reaction is very simple and straightforward to produce BF2bdk with desired functional groups in reasonable isolation ***,when some specific BF2bdk compounds are used as luminescent dopants,organic room-temperature phosphorescence with lifetimes>1.0 s and intense afterglow under ambient conditions can be achieved with the assistance by suitable organic *** dopant-matrix materials exhibit excellent processability into desired patterns and large-area panels,and function as efficient donors of excited state energy transfer for the fabrication of red afterglow materials.

关键词： Afterglow Boron Luminescence Phosphorescence Photophysics

Self‑Healing Dynamic Hydrogel Microparticles with Structural Color for Wound Management

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Nano-Micro Letters 2024年第11期16卷 177-190页

作者： Li Wang xiaoya Ding Lu Fan Anne M.Filppula Qinyu Li Hongbo Zhang Yuanjin zhao Luoran Shang Department of General Surgery Ruijin HospitalShanghai Jiaotong University School of MedicineShanghai 200025People’s Republic of China Department of Rheumatology and Immunology Nanjing Drum Tower HospitalSchool of Biological Science and Medical EngineeringSoutheast UniversityNanjing 210096People’s Republic of China Pharmaceutical Sciences Laboratory Åbo Akademi University20520 TurkuFinland Oujiang Laboratory(Zhejiang Lab for Regenerative Medicine Vision and Brain Health)Wenzhou InstituteUniversity of Chinese Academy of SciencesWenzhou 325001People’s Republic of China Shanghai Xuhui Central Hospital Zhongshan‑Xuhui Hospitaland the Shanghai Key Laboratory of Medical Epigeneticsthe International Co‑laboratory of Medical Epigenetics and Metabolism(Ministry of Science and Technology)Institutes of Biomedical SciencesFudan UniversityShanghai 200032People’s Republic of China

Chronic diabetic wounds confront a significant medical challenge because of increasing prevalence and difficult-healing *** is vital to develop multifunctional hydrogel dressings,with well-designed morphology and structure to enhance flexibility and effectiveness in wound *** achieve these,we propose a self-healing hydrogel dressing based on structural color microspheres for wound *** microsphere comprised a photothermal-responsive inverse opal framework,which was constructed by hyaluronic acid methacryloyl,silk fibroin methacryloyl and black phosphorus quantum dots(BPQDs),and was further re-filled with a dynamic *** dynamic hydrogel filler was formed by Knoevenagel condensation reaction between cyanoacetate and benzaldehyde-functionalized dextran(DEX-CA and DEX-BA).Notably,the composite microspheres can be applied arbitrarily,and they can adhere together upon near-infrared irradiation by leveraging the BPQDs-mediated photothermal effect and the thermoreversible stiffness change of dynamic ***,eumenitin and vascular endothelial growth factor were co-loaded in the microspheres and their release behavior can be regulated by the same ***,effective monitoring of the drug release process can be achieved through visual color *** microsphere system has demonstrated desired capabilities of controllable drug release and efficient wound *** characteristics suggest broad prospects for the proposed composite microspheres in clinical applications.

关键词： Black phosphorus Structural color Dynamic hydrogel Inverse opal Wound management

Melatonin improves synapse development by PI3K/Akt signaling in a mouse model of autism spectrum disorder

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Neural Regeneration Research 2024年第7期19卷 1618-1624页

作者： Luyi Wang Man Xu Yan Wang Feifei Wang Jing Deng xiaoya Wang Yu zhao Ailing Liao Feng Yang Shali Wang Yingbo Li Institute of Neuroscience Department of PhysiologySchool of Basic Medical ScienceChongqing Medical UniversityChongqingChina Department of Pediatric Chongqing University Fuling HospitalChongqingChina Department of Neurosurgery Xinqiao HospitalArmy Medical UniversityChongqingChina Department of Pathology Affiliated Hospital of North Sichuan Medical CollegeNanchongSichuan ProvinceChina NHC Key Laboratory of Birth Defects and Reproductive Health Chongqing Population and Family Planning Science and Technology Research InstituteChongqingChina China National Clinical Research Center for Neurological Diseases Beijing Tiantan HospitalCapital Medical UniversityBeijingChina Advanced Innovation Center for Human Brain Protection Capital Medical UniversityBeijingChina

Autism spectrum disorders are a group of neurodevelopmental disorders involving more than 1100 genes,including Ctnnd2 as a candidate ***2knockout mice,serving as an animal model of autis m,have been demonstrated to exhibit decreased density of dendritic *** role of melatonin,as a neuro hormone capable of effectively alleviating social interaction deficits and regulating the development of dendritic spines,in Ctnnd2 deletion-induced nerve injury remains unclea *** the present study,we discove red that the deletion of exon 2 of the Ctnnd2 gene was linked to social interaction deficits,spine loss,impaired inhibitory neurons,and suppressed phosphatidylinositol-3-kinase(PI3K)/protein kinase B(Akt) signal pathway in the prefrontal *** findings demonstrated that the long-term oral administration of melatonin for 28 days effectively alleviated the aforementioned abnormalities in Ctnnd2 gene-knockout ***,the administration of melatonin in the prefro ntal cortex was found to improve synaptic function and activate the PI3K/Akt signal pathway in this *** pharmacological blockade of the PI3K/Akt signal pathway with a PI3K/Akt inhibitor,wo rtmannin,and melatonin receptor antagonists,luzindole and 4-phenyl-2-propionamidotetralin,prevented the melatonin-induced enhancement of GABAergic synaptic *** findings suggest that melatonin treatment can ameliorate GABAe rgic synaptic function by activating the PI3K/Akt signal pathway,which may contribute to the improvement of dendritic spine abnormalities in autism spectrum disorders.

关键词： autism Ctnnd2 deletion GABAergic neurons melatonin PI3K/Akt signal pathway prefrontal cortex social behavior spine density synaptic-associated proteins

Metabolomic reveals the inhibition mechanism of proline on hexanal production in chicken meat

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Food Science and Human Wellness 2024年

作者： Huanxian Cui Yuxi Jin Yanke Wang xiaoya Yuan Li Liu Jie Wen Guiping zhao State Key Laboratory of Animal Nutrition Institute of Animal Science Chinese Academy of Agricultural Sciences

Hexanal contributes significantly to meat flavor. The aim of this study was to identify the metabolic pathways of hexanal formation in chicken meat. We found 32 metabolites associated with hexanal content in chicken meat， mainly including fatty acids (linoleic acid， etc.， positive correlation) and some amino acids (L-proline， etc.， negative correlation). A comparative analysis of the expression of these 32 metabolites between two groups of chickens with high- and low hexanal content revealed that L-proline and phenylacetaldehyde were downregulated， while ADP-ribose and 4-methylphenol were upregulated in the high-hexanal content chickens. It was also found that the increase of ADP-ribose induced the conversion of L-proline to other amino acids， such as arginine， through a process likely involving the pyrimidine metabolism pathway， leading to a reduction in proline content， which in turn abolished the inhibitory effect of proline on hexanal and enhanced the formation of hexanal. These findings confirm the effect of linoleic acid on hexanal content， and also reveal a negative regulatory effect of proline on the production of hexanal and its elimination mechanism in chicken meat.

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J-family genes redundantly regulate flowering time and increase yield in soybean

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The Crop Journal 2024年第3期12卷 944-949页

作者： Haiyang Li Zheng Chen Fan Wang Hongli Xiang Shuangrong Liu Chuanjie Gou Chao Fang Liyu Chen Tiantian Bu Fanjiang Kong Xiaohui zhao Baohui Liu xiaoya Lin Guangdong Key Laboratory of Plant Adaptation and Molecular Design/Guangzhou Key Laboratory of Crop Gene Editing/Innovative Center of Molecular Genetics and Evolution School of Life SciencesGuangzhou UniversityGuangzhou 510006GuangdongChina

Soybean(Glycine max)is a short-day crop whose flowering time is regulated by *** longjuvenile trait extends its vegetative phase and increases yield under short-day *** variation in J,the major locus controlling this trait,modulates flowering *** report that the three J-family genes influence soybean flowering time,with the triple mutant Guangzhou Mammoth-2 flowering late under short days by inhibiting transcription of E1-family genes.J-family genes offer promising allelic combinations for breeding.

关键词： Soybean Flowering time Yield J-family genes

Lineage reprogramming of fibroblasts into induced cardiac progenitor cells by CRISPR/Cas9-based transcriptional activators

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Acta Pharmaceutica Sinica B 2020年第2期10卷 313-326页

作者： Jianglin Wang Xueyan Jiang Lixin zhao Shengjia Zuo Xiantong Chen Lingmin Zhang Zhongxiao Lin xiaoya zhao Yuyan Qin Xinke Zhou Xi-Yong Yu Key Laboratory of Molecular Target&Clinical Pharmacology and the State Key Laboratory of Respiratory Disease School of Pharmaceutical Sciences&the Fifth Affiliated HospitalGuangzhou Medical UniversityGuangzhou 511436China

Overexpression of exogenous lineage-determining factors succeeds in directly reprogramming fibroblasts to various cell *** studies have reported reprogramming of fibroblasts into induced cardiac progenitor cells(iCPCs).CRISPR/Cas9-mediated gene activation is a potential approach for cellular reprogramming due to its high precision and multiplexing *** we show lineage reprogramming to iCPCs through a dead Cas9(dCas9)-based transcription activation *** and robust activation of endogenous cardiac factors,including GATA4,HAND2,MEF2 C and TBX5(G,H,M and T;GHMT),can reprogram human fibroblasts toward *** iCPCs show potentials to differentiate into cardiomyocytes,smooth muscle cells and endothelial cells in *** of MEIS1 to GHMT induces cell cycle arrest in G2/M and facilitates cardiac *** reprogramming of human fibroblasts into iCPCs provides a promising cellular resource for disease modeling,drug discovery and individualized cardiac cell therapy.

关键词： Lineage reprogramming Human foreskin fibroblasts Induced cardiac progenitor cells CRISPR/Cas9 SAM Cardiac transcription factors

基于FDA-LSTM的冷轧过程多源异构时序数据处理及颤振预测

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振动与冲击 2022年第22期41卷 202-210页

作者：赵潇雅郜志英周晓敏宋寅虎北京科技大学机械工程学院北京100083

冷连轧颤振诱发机理复杂多变,颤振问题的解决需要通过大数据驱动的信息挖掘对机理模型进行补充。该研究针对某冷连轧机现场采集的工艺参数及振动数据,通过函数型数据分析(functional data analysis,FDA)方法进行预处理,实现多源异构时... 详细信息

冷连轧颤振诱发机理复杂多变,颤振问题的解决需要通过大数据驱动的信息挖掘对机理模型进行补充。该研究针对某冷连轧机现场采集的工艺参数及振动数据,通过函数型数据分析(functional data analysis,FDA)方法进行预处理,实现多源异构时序数据的频率协同;采用SelectKBest算法对影响颤振的多种工艺参数进行特征选择,筛选出与振动相关性较强的因素,构造样本空间;基于长短时记忆(long short-term memory,LSTM)神经网络建立振动能量值的预测模型,并与径向基函数(radial basis function,RBF)神经网络、循环神经网络(recurrent neural network,RNN)模型进行比较。结果表明,LSTM模型具有较高的预测精度,同时采用阈值法验证该模型能有效地预测颤振的发生。

关键词：冷连轧颤振多源异构时序数据函数型数据分析(FDA) 长短时记忆(LSTM)神经网络