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Journal of Future Foods 2024年第4期4卷 369-375页

作者： Meirong Cao Jing Wang Mengtian Wang xiaoxuan yuan Xu Zhang Junzhan Ma Yan Zhang Qiang Li Hebei Food Safety Key Laboratory Key Laboratory of Special Food Supervision Technology for State Market RegulationHebei Engineering Research Center for Special Food Safety and HealthHebei Food Inspection and Research InstituteShijiazhuang 050227China Hebei Key Laboratory of Forensic Medicine College of Forensic MedicineHebei Medical UniversityShijiazhuang 050017China

To effectively assess the extent of the mycotoxin contamination of grapes,a method was developed to analyze 22 mycotoxins by ultra-high-performance liquid chromatography coupled with quadrupole/orbitrap high-resolution mass spectrometry(UPLC-Q-Orbitrap HRMS).Samples were extracted and purified using the QuEChERS(quick,easy,cheap,effective,rugged,and safe)*** extracts were separated on a BEH Shield C18 column(100 mm×2.1 mm,1.7μm),using methanol and water with 0.1%formic acid solution and 2 mmol/L ammonium acetate as the mobile *** quantification was performed according to a matrix-matched external standard *** was a good linear relationship within the respective mass concentration ranges of 22 mycotoxins;the correlation coefficients were not less than *** limit of quantification was 0.3-10.0μg/*** recovery ranged from 68.6%to 109.0%,with a relative standard deviation of 1.28%-12.80%.The method is simple,rapid,highly sensitive,and accurate,making it useful for screening grapes for common and emerging mycotoxins.

关键词： Mycotoxins Ultra-high-performance liquid chromatography-quadrupole/orbitrap high-resolution mass spectrometry Grape

雷公藤红素及其衍生物的体外神经保护作用与机制研究

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中国药房 2024年第5期35卷 536-541页

作者：陈佩佩袁晓煊张鑫徐伟许少华福建中医药大学药学院福州350122 中国中医科学院道地药材国家重点实验室北京100700

目的从神经炎症和氧化损伤2个方面探究雷公藤红素(Cel)及其衍生物Cel-1、Cel-2的神经保护作用及可能机制。方法利用1μg/mL脂多糖(LPS)诱导建立小胶质BV2细胞神经炎症模型、200μmol/L过氧化氢(H_(2)O_(2))诱导建立人神经母细胞瘤SH-SY5... 详细信息

目的从神经炎症和氧化损伤2个方面探究雷公藤红素(Cel)及其衍生物Cel-1、Cel-2的神经保护作用及可能机制。方法利用1μg/mL脂多糖(LPS)诱导建立小胶质BV2细胞神经炎症模型、200μmol/L过氧化氢(H_(2)O_(2))诱导建立人神经母细胞瘤SH-SY5Y细胞氧化损伤模型,考察不同浓度(0.625~20μmol/L)Cel、Cel-1、Cel-2对2种细胞的毒性;并在安全浓度(0.039~0.625μmol/L)范围内检测LPS诱导BV2细胞培养液中一氧化氮(NO)、肿瘤坏死因子α(TNF-α)、白细胞介素1β(IL-1β)、IL-6含量,检测H_(2)O_(2)诱导SH-SY5Y细胞的存活率。检测0.156、0.313、0.625μmol/L活性化合物Cel-2作用后H_(2)O_(2)诱导SH-SY5Y细胞中磷脂酰肌醇3激酶(PI3K)、磷酸化PI3K(p-PI3K)、蛋白激酶B(Akt)、磷酸化Akt(p-Akt)、胱天蛋白酶3(caspase-3)、B淋巴细胞瘤2(Bcl-2)和Bcl-2相关X蛋白(Bax)表达水平。结果在0.039~0.625μmol/L浓度梯度区间,神经炎症模型实验显示,Cel、Cel-1、Cel-2均可降低BV2细胞培养液中NO、TNF-α、IL-1β、IL-6的含量(P<0.05或P<0.01),其对神经炎症的半数抑制浓度分别为(0.25±0.04)、(0.61±0.14)、(0.11±0.02)μmol/L;氧化损伤模型实验显示,Cel、Cel-1、Cel-2在一定浓度下可逆转H_(2)O_(2)处理后SH-SY5Y细胞存活率降低的现象(P<0.05或P<0.01),其神经保护的半数效应浓度分别为(0.43±0.08)、(0.45±0.04)、(0.28±0.03)μmol/L。经H_(2)O_(2)诱导,Cel-2干预后SH-SY5Y细胞中PI3K、Akt蛋白的磷酸化水平和Bcl-2蛋白表达水平、Bcl-2/Bax比值均显著升高(P<0.05或P<0.01),caspase-3、Bax蛋白表达水平均显著降低(P<0.05或P<0.01)。结论Cel、Cel-1、Cel-2在一定浓度下均具有显著的神经保护活性,且以Cel-2的活性最强;Cel-2的作用机制可能与调控PI3K/Akt、caspase-3/Bcl-2/Bax信号通路,减轻炎症反应、氧化应激损伤,抑制神经细胞凋亡有关。

关键词：雷公藤红素衍生物神经保护作用神经炎症氧化应激

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Photocatalytic degradation of 4-tert-octylphenol in a spiral photoreactor system

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Journal of Environmental Sciences 2012年第9期24卷 1679-1685页

作者： Yanlin Wu Haixia yuan xiaoxuan Jiang Guanran Wei Chunlei Li Wenbo Dong Department of Environmental Science and Engineering Fudan University Shanghai 200433 Chint~ E-mail: wuyanlin2004@***

A spiral photoreactor system （SPS） was developed for the degradation of 4-tert-octylphenol （4-t-OP） in aqueous phase. 4-t-OP was previously considered as a endocrine disrupting compound frequently present in water. The direct photodegradation reaction caused by the SPS was found to accord with the characteristic of apparent first-order reaction with reaction rate constant k = 4.8 x 10-2 min-1. However, the direct photodegradation reaction could not make the 4-t-OP mineralized. The photodegradation efficiency increased from 88% to 91.2% in 45 min irradiation period after the internal surface of SPS was sintered with TiO2 thin film as catalyst. Catalyst concentration, number of catalyst coating layers and initial concentration of 4-t-OP were proven to be the factors affecting the photocatalytic degradation performance of the SPS on aqueous 4-t-OP. The degradation mechanism was investigated and the byproducts were analyzed using total organic carbon analyzer （TOC） and LC-MS. The possible chemical structures of the products were suggested. SPS with single layer of TiO2 prepared by sintering 13.6% of TiO2 precursor was proven to be more efficient than most of previous systems for removal of 4-t-OP from aqueous phase. 28.3% of the 4-t-OP was mineralized in 45 min according to the decreased amount of TOC value.

关键词： spiral photoreactor system UV light 4-t-OP photocatalytic degradation TiO2

Effect of nitrile butadiene rubber hardness on the sealing characteristics of hydraulic O-ring rod seals

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Journal of Zhejiang University-Science A(Applied Physics & Engineering) 2024年第1期25卷 63-78页

作者： xiaoxuan LI Bingqing WANG Xudong PENG Yuntang LI Xiaolu LI yuan CHEN Jie JIN College of Quality and Safety Engineering China Jiliang UniversityHangzhou310018China College of Mechanical and Electrical Engineering China Jiliang UniversityHangzhou310018China College of Mechanical Engineering Zhejiang University of TechnologyHangzhou310023China

The nitrile butadiene rubber(NBR)hardness effect on the sealing characteristics of hydraulic O-ring rod seals is analyzed based on a mixed lubrication elastohydrodynamic *** studies are conducted to reveal the mechanism of the influence of rubber hardness on the static and dynamic behavior of *** optimized selections of rubber hardness are then investigated under different *** show that the low hardness seal is prone to stress concentration due to the extrusion effect under high pressure conditions;it is also more prone to leaking.A high hardness seal can better prevent leakage by reducing film thickness but it will cause large frictional power loss and increase the probability of wear *** choice of low hardness is recommended to reduce friction with the premise that leakage requirements are met.

关键词： Nitrile butadiene rubber(NBR)hardness Sealing characteristics Optimized selection O-ring seal

GRB 210323A:Signature of Long-lasting Lifetime of Supra-massive Magnetar as the Central Engine from the Merger of Binary Neutron Star

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Research in Astronomy and Astrophysics 2024年第8期24卷 32-41页

作者： Yingze Shan xiaoxuan Liu Xing Yang Haoyu yuan Houjun Lü Guangxi Key Laboratory for Relativistic Astrophysics School of Physical Science and TechnologyGuangxi UniversityNanning 530004China Department of Astronomy School of PhysicsHuazhong University of Science and TechnologyWuhan 430074China

Theoretically,a supra-massive neutron star or magnetar may be formed after the merger of binary neutron ***210323A is a short-duration gamma-ray burst(GRB)with a duration of lasting~1 *** light curve of the prompt emission of GRB 210323A shows a signal-peaked structure and a cutoff power-law model can adequately fit the spectra with E_p=1826±*** interestingly,it has an extremely long-lasting plateau emission in the X-ray afterglow with a duration of~10^(4)s,and then follows a rapid decay with a decay slope~*** temporal feature is challenging by invoking the external shock *** this paper,we suggest that the observed long-lasting X-ray plateau emission is caused by the energy injection of dipole radiation from supra-massive magnetar,and the abrupt decay following the longlasting X-ray plateau emission is explained by supra-massive magnetar collapsing into a black *** is the short GRB(SGRB)with the longest X-ray internal plateau emission powered by a supra-massive neutron *** this is the case,one can estimate the physical parameters of a supra-massive magnetar,and compare with other *** also discuss the possible gravitational-wave emission,which is powered by a supra-massive magnetar and its detectability,and the possible kilonova emission,which is powered by r-process and magnetar spin-down to compare with the observed data.

关键词： (stars)gamma-ray burst general-(stars)gamma-ray burst individual(GRB 210323A)-stars magnetars

Journal of Genetics and Genomics in 2023:progresses and beyond

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Journal of Genetics and Genomics 2024年第1期51卷 1-2页

作者： Xiu-Fen Song xiaoxuan Guo Jing Zhao Yutian Zhang yuan Qin Jianru Zuo Journal of Genetics and Genomics Institute of Genetics and Developmental BiologyChinese Academy of Sciences&the Genetics Society of ChinaBeijing 100101China

Journal of Genetics and Genomics(JGG),launched in 1974,celebrates its 50th birthday in *** continuous support from our authors,reviewers,readers,and the editorial board,JGG has made remarkable progress in the past year by publishing 122 papers covering major disciplines in life sciences and medical genetics with exciting *** is worthwhile to note that we have recruited 66 young talents to join JGG as junior editors,resulting in significant improvement in manuscript evaluation and journal ***,we summarize major progresses reported in JGG in 2023 fromaneditorial view.

关键词： journal beyond promotion

Bacteriophages assisted bacteria to facilitate soil multifunctionality under organochlorine pesticide contamination

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Science China Technological Sciences 2024年第10期67卷 3116-3128页

作者： ZHAO DuoKai CHAO HuiZhen BALCAZAR Jose Luis WU ShiMao ZHENG xiaoxuan WU YunLing yuan ShuJian SUN MingMing HU Feng Soil Ecology Lab Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization and Jiangsu Key Laboratory for Solid Organic Waste UtilizationNanjing Agriculture University J.F.Blumenbach Institute of Zoology and Anthropology University of G?ttingen Catalan Institute for Water Research (ICRA) University of Girona College of Environmental and Resource Sciences Zhejiang University

As the most abundant living entities in the environment, viruses have been well recognized as crucial members in sustaining biogeochemical cycling. However, the significance of viruses in soil ecosystem multifunctionality remains under-explored. In this study, we used metagenomics and meta-viromics analysis to investigate the role of soil viruses in soil ecosystem functions under heavy, light, and no organochlorine pesticides(OCPs) contamination. In the three types of soil samples collected, lightcontaminated soils supported the highest level of multifunctionality, followed by heavy-contaminated soils and clean ***, our results revealed a positive correlation between bacterial community evenness and multifunctionality index(p <0.05). Dominant bacterial species with biodegradation and stress resistance advantages exhibited higher abundance in OCPaffected soils, potentially playing a core functional supporting role. Furthermore, our results indicated that the species richness and diversity of bacteriophages were positively correlated with multifunctionality(p < 0.05) in OCP-affected soils. Bacteriophages in OCP-affected soils regulate host metabolism and enhance soil ecosystem multifunctionality by infecting functional bacterial hosts and encoding AMGs related to soil element cycling. Our findings emphasize the potential effect of phages on ecosystem multifunctionality in contaminated soil, suggesting that phages may serve as contributors to soil ecology beyond bacteria and other microorganisms. Therefore, in polluted or constrained soils, further research could potentially translate phage communities and related ecological processes into artificial methods for application in soil pollution remediation or ecological restoration.

关键词： soil ecosystem multifunctionality viruses auxiliary metabolic genes organochlorine pesticides contamination

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论网络舆论对传统媒体舆论的优化

新闻界 2010年第2期 81-83页

作者：袁小轩深圳信息职业技术学院计算机应用系深圳518029

本文从三个方面论述了网络舆论对传统媒体舆论的优化作用。

关键词：网络舆论传统媒体优化

Targeting a disintegrin and metalloprotease(ADAM)17-CD122 axis enhances CD8^(+)T cell effector differentiation and anti-tumor immunity

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Signal Transduction and Targeted Therapy 2024年第7期9卷 3097-3111页

作者： Lina Sun Anjun Jiao Haiyan Liu Renyi Ding Ning yuan Biao Yang Cangang Zhang xiaoxuan Jia Gang Wang Yanhong Su Dan Zhang Lin Shi Chenming Sun Aijun Zhang Lianjun Zhang Baojun Zhang Department of Pathogenic Microbiology and Immunology School of Basic Medical SciencesXi’an Jiaotong UniversityXi’anShaanxi 710061China Institute of Infection and Immunity Translational Medicine InstituteXi’an Jiaotong University Health Science CenterXi’anShaanxi 710061China Key Laboratory of Environment and Genes Related to Diseases Ministry of EducationXi’anShaanxi 710061China Xi’an Key Laboratory of Immune Related Diseases Xi’anShannxi 710061China Jiangsu Center for the Collaboration and Innovation of Cancer Biotherapy Cancer InstituteXuzhou Medical UniversityXuzhouJiangsuChina Department of Pediatrics Qilu Hospital of Shandong UniversityJinanChina National Key Laboratory of Immunity and Inflammation Suzhou Institute of Systems MedicineChinese Academy of Medical Sciences&Peking Union Medical CollegeSuzhou 215123 JiangsuChina Key Laboratory of Synthetic Biology Regulatory Elements Suzhou Institute of Systems MedicineChinese Academy of Medical Sciences&Peking Union Medical CollegeSuzhou 215123 JiangsuChina

CD8^(+)T cell immune responses are regulated by multi-layer networks,while the post-translational regulation remains largely *** ectodomain shedding is an important post-translational process orchestrating receptor expression and signal transduction through proteolytic cleavage of membrane ***,by targeting the sheddase A Disintegrin and Metalloprotease(ADAM)17,we defined a post-translational regulatory mechanism mediated by the ectodomain shedding in CD8^(+)T *** and proteomic analysis revealed the involvement of post-translational regulation in CD8^(+)T cells.T cellspecific deletion of ADAM17 led to a dramatic increase in effector CD8^(+)T cell differentiation and enhanced cytolytic effects to eliminate pathogens and ***,ADAM17 regulated CD8^(+)T cells through cleavage of membrane ***17 inhibition led to elevated CD122 expression and enhanced response to IL-2 and IL-15 stimulation in both mouse and human CD8^(+)T ***,inhibition of ADAM17 in CD8^(+)T cells improved the efficacy of chimeric antigen receptor(CAR)T cells in solid *** findings reveal a critical post-translational regulation in CD8^(+)T cells,providing a potential therapeutic strategy of targeting ADAM17 for effective anti-tumor immunity.

关键词： immunity stimulation involvement

电视节目制作专业经常性项目导向下的任务驱动教学初探

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深圳信息职业技术学院学报 2009年第1期7卷 14-18页

作者：袁小轩深圳信息职业技术学院计算机应用系广东深圳518029

本文以深圳信息职业技术学院电视节目制作专业为例,探索了一种切合本专业特点的新型教学模式——经常性项目导向下的任务驱动教学,分析了新型教学模式的构成以及教学管理改善等问题。

关键词：工学结合项目导向任务驱动经常性项目