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Autoantigen Microarray for High-throughput Autoantibody Profiling in Systemic Lupus Erythematosus

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Genomics, Proteomics & Bioinformatics 2015年第4期13卷 210-218页

作者： Honglin Zhu Hui Luo Mei Yan xiaoxia zuo Quan-Zhen Li Department of Rheumatology and Immunology Xiangya Hospital Central South University Department of Immunology and Internal Medicine University of Texas Southwestern Medical Center

Systemic lupus erythematosus （SLE） is a complex autoimmune disease characterized by the production of autoantibodies to a broad range of self-antigens. Profiling the autoantibody repertoire using array-based technology has emerged as a powerful tool for the identification of biomarkers in SLE and other autoimmune diseases. Proteomic microarray has the capacity to hold large number of self-antigens on a solid surface and serve as a high-throughput screening method for the determination of autoantibody specificities. The autoantigen arrays carrying a wide variety of self-antigens, such as cell nuclear components （nucleic acids and associated proteins）, cytoplas- mic proteins, phospholipid proteins, cell matrix proteins, mucosal/secreted proteins, glomeruli, and other tissue-specific proteins, have been used for screening of autoantibody specificities associated with different manifestations of SLE. Arrays containing synthetic peptides and molecular modified proteins are also being utilized for identification of autoantibodies targeting to special antigenic epi- topes. Different isotypes of autoantibodies, including IgG, IgM, IgA, and IgE, as well as other Ig subtypes, can be detected simultaneously with multi-color labeled secondary antibodies. Serum and plasma are the most common biologic materials for autoantibody detection, but other body fluids such as cerebrospinal fluid, synovial fluid, and saliva can also be a source of autoantibody detection.

关键词： Systemic lupus erythemato-sus(SLE) Autoantibody profiling Proteomic microarray Biomarker High-throughput assay

Synthesis, Structural Characterization,and Electrochemical Properties of Binuclear Copper(Ⅱ) Complexes Containing Tetradentate Schiff Base Ligand

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Wuhan University Journal of Natural Sciences 2010年第2期15卷 165-170页

作者： DING Yu HU xiaoxia SUN Xiaoli zuo Zhiling School of Chemistry and Materials Science Xiaogan UniversityXiaogan 432000 Hubei China

Two binuclear copper（Ⅱ） complexes, [Cu2（saloph）2 （μ-O）2]·2（DMF）[Hasaloph N,N＇-o-phenylenebis（salicylideneaminato）] （a） and [Cu2（salen）2（μ-O）2] [H2salen=N,N＇-bis（salicylic-deneaminato） ethylene] （b）were synthesized and characterized by X-ray crystal-lography. Both of them have distorted rectangular pyramidal geometry around Cu（Ⅱ）. The complete series of complexes show bridging phenoxo groups between the copper centers, together with hydroxo-bridges in these complexes. The complexes have also been characterized by elemental analysis, IR, TG-DTA, and electrochemical results.

关键词： copper(Ⅱ) complexes crystal structure electro-chemical properties

A combined genome-wide DNA methylation and transcription analysis identified genes related with pathogenesis of systemic sclerosis

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A combined genome-wide DNA methylation and transcription ana...

A combined genome-wide transcription analysis and DNA methylation identified genes related with pathogenesis of lupus nephritis

第十届全国免疫学学术大会

作者： Honglin Zhu Hui Luo Tao Chen Shengxi Liu Quanzhen Li xiaoxia zuo Department of Rheumatology Xiangya Hospital Central South University Department of Immunology and Internal Medicine University of Texas Southwestern Medical Center 5323 Harry Hines BlvdDallas

Objectives:Systemic sclerosis(SSc)is an autoimmune disease with unknown *** have revealed epigenetic modification can contribute to susceptibility and pathogenic process in systemic autoimmune diseases by regulating gene activation or *** DNA methylation has been reported in dermal fibroblasts of SSc patients,but little is known about the DNA methylation changes in relation with gene expression on a genome-wide scale in ***:A genome-wide DNA methylation and transcription analysis were performed on PBMC from 19 SSc patients and 18 matched healthy controls(HC)using the Illumina Human Methy450 and Human HT-12 *** serum levels of proinflammatory cytokines were also analyzed by the Bio-plex human cytokine 27-plex *** methylated Cp G sites and genes between patients and controls were identified with Genomstudio,R and SAM *** association between DNA Methylation and gene expression were *** interaction pathways of the differentially expressed genes were analyzed by IPA ***:Out of 485,000 Cp G sites across the genome,4,848 hypomethylated and 216 hypermethylated Cp G sites were identified from SSc as compared with HC,representing 2,488 hypomethylated and 134 hypermethylated *** microarray analysis identified 1,023 significantly upregulated genes in SSc,of which 405 genes were hypomethylated,indicating a negative regulation of DNA methylation on gene *** hypomethylated genes in SSc include established autoimmune-related and novel genes,such as AIF1,ARID3A,IFI44L,PARP9,RSAD2,EPSTI1,EIF2AK2 and *** analysis revealed enrichment of genes involved in Wnt signaling pathway,leukocyte transendothelial migration,cell adhesion molecules and chemokine signaling *** Wnt signaling pathway was represented by hypomethylation of Wnt5b,Wnt7a,Smad2,and *** hypomethylated genes involved in chemokine signaling pathway were correlated with the increas

关键词： Systemic sclerosis DNA methylation gene expression

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A combined genome-wide transcription analysis and DNA methyl...

儿童青少年肥胖伴原发性高血压营养干预的研究

第十届全国免疫学学术大会

作者： Honglin Zhu Luo Hui Chen Tao Shengxi Liu Quanzhen Li xiaoxia zuo Department of Rheumatology Xiangya Hospital Central South University Department of Immunology and Internal Medicine University of Texas Southwestern Medical Center 5323 Harry Hines Blvd

Objectives:Lupus nephritis(LN)is one of the most devastating complications and a main cause of morbidity and mortality in systemic lupus erythematosus(SLE).Genetic,hormonal and environment factors and its complexity interaction contributed to the pathogenitic mechanisms of SLE,but the etiology of LN is not clear,a better understand the pathogenesis of LN is ***:A genome-wide transcription analysis and DNA methylation were performed on PBMC from 30 SLE patients(15 SLE with LN,15 SLE without LN)and 25 matched normal controls(NC)using the Human HT-12 Beadchips and Illumina Human *** autoantibodies were detected by using 98 autoantigen *** proinflammatory cytokines were analyzed by Bioplex human cytokine 27 plex *** methylated Cp G sites and genes were identified with Genomstudio,R and SAM *** was analyzed by IPA ***:24 antinuclear antibodies titers were markedly higher in SLE patients,most of them were even higher in SLE patients with *** expression and DNA methylation profiling suggests common changes found in the two groups of *** genes participated in interferon Signaling,recognition of bacteria and viruses,T cell and B cell signaling and et *** were 34 different expressed genes in SLE with LN compared with SLE without LN.6 genes were up-regulated and 28 genes were down-regulated in SLE with LN.E2F2 and MX1 was upregulated and *** concentrate of inflammatory cytokines IL17A,IP10,BASIC-FGF,TNFα,IL6,IL15,GM-CCSF,IL1RA,IL5 and IL12P70 were significant higher in SLE compared with NC,but there was no significant difference between the two groups of *** expressions of these cytokines were changed *** with cytokine methylation data,IL5 gene expression was down-regulated and hypomethylated;TNFαgene expression was up-regulated and ***:We provide a comprehensive analysis of autoantibodies,gene expression,methylation

关键词： Lupus nephritis systemic lupus erythematosus DNA methylation Gene expression

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重庆医科大学学报 2012年第2期37卷 177-179页

作者：左小霞王晶张晔解放军第309医院营养科北京100091

目的:研究肥胖儿童高血压的发病率及经合理饮食干预、运动等综合治疗后的转归,为儿童青少年高血压的防治提供营养治疗指导。方法:以72例6～18岁的肥胖伴高血压儿童青少年为研究对象。总热能根据年龄、性别、身高及肥胖程度制定,一般为4 ... 详细信息

目的:研究肥胖儿童高血压的发病率及经合理饮食干预、运动等综合治疗后的转归,为儿童青少年高血压的防治提供营养治疗指导。方法:以72例6～18岁的肥胖伴高血压儿童青少年为研究对象。总热能根据年龄、性别、身高及肥胖程度制定,一般为4 180～7 524 kJ/d,高蛋白质、脂肪和碳水化合物三者占总热能的百分比分别为20%～25%、25%～30%和45%～50%。除营养干预外,每天运动不少于30 min并常规补充多种维生素及微量元素制剂。结果:经以合理营养干预为主的综合治疗后,体质指数(Body mass index,BMI)、收缩压、舒张压较治疗前明显下降(P<0.05),高血压的痊愈率为79.2%(57/72),好转率为16.7%(12/72),未愈率为4.1%(3/72)。结论:以合理营养干预为主的综合治疗对儿童青少年肥胖伴原发性高血压(Essential hyperten-sion,EH)的防治具有明显疗效。

关键词：儿童青少年肥胖高血压营养干预

系统性硬化症合并强直性脊柱炎1例及文献复习（英文）

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中南大学学报（医学版） 2018年第11期43卷 1263-1265页

作者：胡淑琳左晓霞李懿莎中南大学湘雅医院风湿免疫科中南大学风湿免疫病研究所长沙410008

系统性硬化症是一种以皮肤增厚和纤维化为特征的自身免疫性疾病,强直性脊柱炎是一种以中轴关节受累为主的关节炎,强直性脊柱炎极少与弥漫性结缔组织病合并存在。既往文献报道了5例强直性脊柱炎合并系统性硬化症的病例,其中仅1例患者诉... 详细信息

系统性硬化症是一种以皮肤增厚和纤维化为特征的自身免疫性疾病,强直性脊柱炎是一种以中轴关节受累为主的关节炎,强直性脊柱炎极少与弥漫性结缔组织病合并存在。既往文献报道了5例强直性脊柱炎合并系统性硬化症的病例,其中仅1例患者诉有肌无力,且5例患者的血清肌酸激酶水平均无明显异常。本文报道1例青年男性患者符合系统性硬化症与强直性脊柱炎的诊断标准,同时出现肌无力、肌酸激酶水平明显增高。人类白细胞抗原(human leukocyte antigen,HLA)分型检测发现该患者携带这两种疾病的HLA易感基因。对该患者予以强的松30 mg/d及环磷酰胺治疗,2个月后皮肤增厚好转,肌酸激酶降至正常。

关键词：系统性硬化症强直性脊柱炎肌酸激酶

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以尿崩症起病的脂质肉芽肿病1例

中华内科杂志 2024年第7期63卷 699-701页

作者：黄婧郭慕瑶罗卉左晓霞中南大学湘雅医院风湿免疫科湖南省风湿免疫病临床医学研究中心国家老年疾病临床医学研究中心(湘雅医院)长沙410008

患者男性60岁,病程1年余。以垂体占位引起的中枢性尿崩症起病,伴主动脉周围软组织包绕、腹膜后病变、渗出性多浆膜腔积液、长骨受累,临床表现类似IgG 4相关性疾病。查血IgG 4浓度正常(0.928 g/L)。左胫骨上段骨病变组织活检病理检查,确... 详细信息

患者男性60岁,病程1年余。以垂体占位引起的中枢性尿崩症起病,伴主动脉周围软组织包绕、腹膜后病变、渗出性多浆膜腔积液、长骨受累,临床表现类似IgG 4相关性疾病。查血IgG 4浓度正常(0.928 g/L)。左胫骨上段骨病变组织活检病理检查,确诊脂质肉芽肿病(Erdheim-Chester病)。予泼尼松(50 mg每日1次)、沙利度胺(100 mg每晚1次)、去氨加压素(0.05 g每日1次)治疗,1个月后患者气促、多尿症状改善,随访半年患者胸腔积液未再增多。通过对本例的分析和总结,临床医生应提高对脂质肉芽肿病这类少见疾病的关注和认识,了解其与IgG 4相关性疾病的鉴别要点,降低误诊和漏诊率。

关键词： IgG 4相关性疾病 Erdheim-Chester病中枢性尿崩症

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狼疮性膀胱炎2例并文献复习

中南大学学报（医学版） 2011年第8期36卷 813-816页

作者：李懿莎罗卉谢艳莉左晓霞中南大学湘雅医院风湿免疫科长沙410008

目的:提高对狼疮性膀胱炎的认识。方法:回顾性分析2例狼疮性膀胱炎患者的临床表现、实验室检查与影像学检查结果,并复习国内报道的6例病例。结果:2例患者均为女性,尿路症状在肠道症状之后出现,均有输尿管扩张和肾积水,1例合并假性肠梗阻... 详细信息

目的:提高对狼疮性膀胱炎的认识。方法:回顾性分析2例狼疮性膀胱炎患者的临床表现、实验室检查与影像学检查结果,并复习国内报道的6例病例。结果:2例患者均为女性,尿路症状在肠道症状之后出现,均有输尿管扩张和肾积水,1例合并假性肠梗阻,糖皮质激素与环磷酰胺治疗效果好。复习文献中的6例患者均有输尿管扩张和肾积水,4例合并假性肠梗阻。结论:当系统性红斑狼疮患者出现尿路症状或肠道症状时,应考虑狼疮性膀胱炎的可能性,积极完善相关检查,予以糖皮质激素和免疫抑制剂治疗。

关键词：系统性红斑狼疮膀胱炎治疗

改良性融合性集合指标在间歇性外斜视中的应用

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中华眼科杂志 2024年第1期60卷 56-63页

作者：李天玺张童左晓霞刘虎王子衿南京医科大学第一附属医院眼科南京210029

目的探讨改良性融合性集合指标——融合性集合幅度/斜视度数与间歇性外斜视患儿的外斜视控制情况、立体视功能及其他视功能之间的关系。方法横断面研究。纳入2020年8月至2021年6月在南京医科大学第一附属医院诊断为间歇性外斜视的患儿... 详细信息

目的探讨改良性融合性集合指标——融合性集合幅度/斜视度数与间歇性外斜视患儿的外斜视控制情况、立体视功能及其他视功能之间的关系。方法横断面研究。纳入2020年8月至2021年6月在南京医科大学第一附属医院诊断为间歇性外斜视的患儿。将同视机法测量的融合性集合幅度与看远三棱镜法测量的斜视度数结合,获得改良性指标——融合性集合幅度/斜视度数。使用间歇性外斜视控制评分评估患儿看远及看近时的外斜视控制情况,分为控制良好(评分0~1)和控制较差(评分2~5)。采用Spearman相关分析、Mann-Whitney U检验、Fisher精确检验、χ^(2)检验、Logistic回归分析和中介效应分析进行统计学处理。结果本研究共纳入212例患儿,其中105例(49.5%)为男性,107例(50.5%)为女性;年龄为9.0(8.0,10.0)岁;201例(94.8%)有双眼融合视功能,11例(5.2%)无双眼融合视功能。有双眼融合视功能的患儿中,融合性集合幅度/斜视度数与看远看近外斜视控制评分均呈负相关(看远:r=-0.427,P<0.001;看近:r=-0.194,P=0.006)。融合性集合幅度/斜视度数是看远(OR=0.195;95%CI:0.060~0.630;P=0.006)和看近(OR=0.252;95%CI:0.085~0.746;P=0.013)外斜视控制情况的独立影响因素。看远(OR=3.110;95%CI:1.311~7.379;P=0.010)和看近(OR=2.780;95%CI:1.401~5.517;P=0.003)立体视功能也是看远外斜视控制情况的影响因素。但中介分析显示,立体视功能不是融合性集合幅度/斜视度数与看远外斜视控制情况之间关联的中介因素(看远:P=0.066;看近:P=0.181)。15例融合性集合幅度/斜视度数≥1.5°/PD的患儿均表现为外斜视控制良好。相反,融合性集合幅度/斜视度数<1.5°/PD的患儿表现为外斜视控制更差(看远:P=0.001;看近:P=0.040)、斜视度数更大(看远:P<0.001;看近:P<0.001)。结论改良性融合性指标——融合性集合幅度/斜视度数结合了同视机与三棱镜的测量结果,可用于评估间歇性外斜视严重程度。融合性集合幅度/斜视度数越大,看远和看近外斜视控制情况可能越好。尽管立体视功能也与看远外斜视控制情况相关,但不是融合性集合幅度/斜视度数与看远外斜视控制情况之间关联的中介因素。

关键词：外斜视会聚,眼深度知觉视觉,双眼视敏度疾病严重程度指数