检索结果-南通市图书馆

稀有金属材料与工程 2006年第A3期35卷 389-391页

作者： xiaowen xu xiaohao wang fei tang zhaoying zhou State Key Laboratory of Precision Measurement Technology and Instruments Tsinghua University Beijing 100084 China

An amperometric biosensor based on acetylcholinesterase (ACHE) is assembled by simple adsorption of the AChE on 7,7,8,8-tetracyanoquinodimethane (TCNQ) modified screen-printed *** biosensor is used to detect the inhibitory effect of organophosphorus pesticides on AChE *** detection of organophosphorus pesticides is done with acetylthiocboline chloride (ATCh) as *** order to obtain the optimized response to substrate,the influencing factors of the biosensor are investigated,including temperature,pH,incubation time,substrate concentration and AChE concentration. The measurements were performed after inhibition by immersing the enzyme electrode into the parathion methyl solution. Under the optimized conditions,that is,500 U/ml AChE concentration,pH 7.2,10 min incubation time,2 mmol/L substrate concentration and temperature of 37℃,from 5×10^(-8) mol/L to 5×10^(-5) mol/L is close to linear (regression equation: y(%)=124.055+15.7991gx,R^2=0.99644),which corresponds to 8.7%～56.1% AChE *** the optimized conditions, the lowest detectable amount of parathion methyl is 13×10^(-9).

关键词： biosensor AChE screen-printed electrodes influencing factors organophosphorus pesticides

Disposable Amperometric Acetylcholinesterase Biosensor for the Detection of Organophosphorus Pesticides

在线全文

学校读者我要写书评

暂无评论

稀有金属材料与工程 2006年第A3期35卷 333-336页

作者： xiaohao wang xiaowen xu fei tang zhaoying zhou State Key Laboratory of Precision Measurement Technology and Instruments Tsinghua University Beijing 100084 China

A rapid,simple,disposable and inexpensive acetylcholinesterase (ACHE) amperometric biosensor for the detection of organophosphorus pesticides was developed by simple adsorption of the enzyme on screen-printed *** biosensor consisted of an Ag/AgCl reference electrode and a graphite working *** mixture of graphite and the 7,7,8,8-tetracyanoquinodimethane (TCNQ) was printed on *** detection of organophosphorus pesticides was done with acetylthiocholine chloride (ATCh) as *** biosensor was used to detect the inhibitory effect of organophosphorus pesticides on AChE *** 1μl of enzyme solution containing 0.1 U AChE and 1% bovine serum albumin (BSA) were simply dropped on the working electrode *** biosensor operated at a potential of 300 mV vs. Ag/AgCl in a pH 7.2 0.1 mol/L phosphate buffer and 0.1 mol/L *** obtained a calibration plot of the percentage inhibition versus the logarithm of parathion methyl concentration following an incubation time of 10 mix in parathion methyl solution. The lowest detectable amount of parathion methyl was 0.026 *** amperometric biosensor based on acetylcholinesterase was disposable and low cost (about 1 yuan RMB).

关键词： amperometric biosensor AChE screen-printed electrodes organophosphorus pesticides

Development of Disposable Amperometric Cholesterol Biosensor

在线全文

学校读者我要写书评

暂无评论

稀有金属材料与工程 2006年第A3期35卷 321-323页

作者： xiaohao wang fei tang xiaowen xu Zhencheng Chen State Key Laboratory of Precision Measurement Technology and Instruments Tsinghua University Beijing 100084 China

A novel disposable cholesterol biosensor based on electrochemical process is developed adopting screen printing *** system consists of a graphite reference electrode and a working *** mixture of cholesterol oxidase and K_4Fe (CN)_6 is printed on the *** biosensor determines total cholesterol content through indirect determination of free cholesterol in the whole human *** 0.3V voltage is applied to the electrodes,the range of detection is from 250 mg/L to 2000 mg/L,with the correlation coefficient of *** total detective duration is less than 200s,and the sample volume is only 4μL.

关键词： cholesterol biosensor carbon electrode screen printing

在线全文

学校读者我要写书评

暂无评论

代谢热整合法无创血糖检测技术研究

仪器仪表学报 2007年第10期28卷 1857-1860页

作者：唐飞王晓浩王东生李俊峰徐效文清华大学精密仪器与机械学系精密测试技术与仪器国家重点实验室北京100084

介绍了一种新的基于代谢热整合法的无创血糖检测技术。采用温度传感器、红外传感器、湿度传感器和光学测量装置,通过测量人体代谢产生的热量、血液流速、血氧饱和度,利用人体代谢产生的热量是血糖浓度、供氧量的函数,可以推算出血糖浓... 详细信息

介绍了一种新的基于代谢热整合法的无创血糖检测技术。采用温度传感器、红外传感器、湿度传感器和光学测量装置,通过测量人体代谢产生的热量、血液流速、血氧饱和度,利用人体代谢产生的热量是血糖浓度、供氧量的函数,可以推算出血糖浓度。介绍了系统的组成、数据处理方法及可能造成误差的因素。用所研制的检测系统进行了临床实验,得到的血糖值与用大型生化分析仪测得的血糖值的相关系数达到0.856。实验证明,代谢热整合法无创血糖检测技术是可行的。

关键词：血糖无创检测代谢热整合法