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Chinese Medical Journal 2024年第2期137卷 140-151页

作者： Minghao Lin xiaosu zhao Yingjun Chang Xiangyu zhao Peking University People’s Hospital Peking University Institute of HematologyNational Clinical Research Center for Hematologic DiseaseBeijing Key Laboratory of Hematopoietic Stem Cell TransplantationBeijing 100044China

Chimeric antigen receptor(CAR)-modified T-cell therapy has achieved remarkable success in the treatment of acute lymphoblastic leukemia(ALL).Measurable/minimal residual disease(MRD)monitoring plays a significant role in the prognostication and management of patients undergoing CAR-T-cell therapy.Common MRD detection methods include flow cytometry(FCM),polymerase chain reaction(PCR),and next-generation sequencing(NGS),and each method has advantages and limitations.It has been well documented that MRD positivity predicts a poor prognosis and even disease relapse.Thus,how to perform prognostic evaluations,stratify risk based on MRD status,and apply MRD monitoring to guide individual therapeutic decisions have important implications in clinical practice.This review assesses the common and novel MRD assessment methods.In addition,we emphasize the critical role of MRD as a prognostic biomarker and summarize the latest studies regarding MRD-directed combination therapy with CAR-T-cell therapy and allogeneic hematopoietic stem cell transplantation(allo-HSCT),as well as other therapeutic strategies to improve treatment effect.Furthermore,this review discusses current challenges and strategies for MRD detection in the setting of disease relapse after targeted therapy.

关键词： Measurable/minimal residual disease Acute lymphoblastic leukemia Chimeric antigen receptor-modified T-cell therapy Allogeneic hematopoietic stem cell transplantation Relapse

Demands and technical developments of clinical flow cytometry with emphasis in quantitative,spectral,and imaging capabilities

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Nanotechnology and Precision Engineering 2022年第4期5卷 46-55页

作者： Ting Zhang Mengge Gao Xiao Chen Chiyuan Gao Shilun Feng Deyong Chen Junbo Wang xiaosu zhao Jian Chen State Key Laboratory of Transducer Technology Aerospace Information Research InstituteChinese Academy of SciencesBeijing 100190People’s Republic of China School of Future Technology University of Chinese Academy of SciencesBeijing 100049People’s Republic of China Peking University People’s Hospital Peking University Institute of HematologyNational Clinical Research Center for Hematologic DiseaseBeijing Key Laboratory of Hematopoietic Stem Cell TransplantationBeijing 100044People’s Republic of China State Key Laboratory of Transducer Technology Shanghai Institute of Microsystem and Information TechnologyChinese Academy of SciencesShanghai 200050People’s Republic of China School of Electronic Electrical and Communication EngineeringUniversity of Chinese Academy of SciencesBeijing 100049People’s Republic of China

As the gold-standard method for single-cell analysis,flow cytometry enables high-throughput and multiple-parameter characterization of individual biological cells.This review highlights the demands for clinical flow cytometry in laboratory hematology(e.g.,diagnoses of minimal residual disease and various types of leukemia),summarizes state-of-the-art clinical flow cytometers(e.g.,FACSLyricTMby Becton Dickinson,DxFLEX by Beckman Coulter),then considers innovative technical improvements in flow cytometry(including quantitative,spectral,and imaging approaches)to address the limitations of clinical flow cytometry in hematology diagnosis.Finally,driven by these clinical demands,future developments in clinical flow cytometry are suggested.

关键词： Clinical demand Clinical flow cytometry Quantitative flow cytometry Spectral flow cytometry Imaging flow cytometry

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p53基因在血液肿瘤中的临床意义

白血病·淋巴瘤 2024年第4期33卷 245-248页

作者：王悦冰赵晓甦北京大学人民医院血液科北京北京大学人民医院血液科北京大学血液病研究所国家血液系统疾病临床医学研究中心北京

p53基因是重要的抑癌基因, 具有调节细胞周期、DNA修复、诱导细胞凋亡等作用。随着基因检测技术的进展, 血液病的分子遗传学特征使得疾病分型和预后分层进一步精细化。近年研究发现, 在血液系统肿瘤中p53突变与不良结局相关, 各指南也... 详细信息

p53基因是重要的抑癌基因, 具有调节细胞周期、DNA修复、诱导细胞凋亡等作用。随着基因检测技术的进展, 血液病的分子遗传学特征使得疾病分型和预后分层进一步精细化。近年研究发现, 在血液系统肿瘤中p53突变与不良结局相关, 各指南也对相关内容有所更新。文章综述了p53基因在血液系统肿瘤中的临床意义, 聚焦于基因突变的异质性如何影响诊治和预后。

关键词： p53基因血液肿瘤基因组结构变异细胞遗传学预后

Improved clinical outcomes of rhG-CSF-mobilized blood and marrow haploidentical transplantation compared to propensity score-matched rhG-CSF-primed peripheral blood stem cell haploidentical transplantation:a multicenter study

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Science China(Life Sciences) 2016年第11期59卷 1139-1148页

作者： Xiangyu zhao Feng Gao Xiaohui Zhang Yu Wang Lanping Xu Kaiyan Liu xiaosu zhao Yingjun Chang Han Wei Huan Chen Yuhong Chen Zhengfan Jiang Xiaojun Huang Peking University People's Hospital&Institute of Hematology Beijing Key Laboratory of Hematopoietic Stem Cell TransplantationBeijing 100044China North China University of Science and Technology Affiliated Hospital Tangshan 063000China Peking University Qinghe Hospital Beijing 100085 China State Key Laboratory of Protein and Plant Gene Research Key Laboratory of Cell Proliferation and Differentiation of the Ministry of EducationSchool of Life SciencesPeking UniversityBeijing 100871China Peking-Tsinghua Center for Life Sciences Beijing 100084China

The effects of haploidentical rhG-CSF-mobilized blood and marrow transplantation(HBMT) on hematological malignances are well established. Previous prospective single-center studies have demonstrated better survival after HBMT versus haploidentical rhG-CSF-mobilized peripheral blood stem cell transplantation(HPBSCT) for acute leukemia(AL) not in remission(NR) or in more than the second complete remission(>CR2). To test the hypothesis that HBMT is still superior to HPBSCT for patients with AL, multiple myeloma(MM), or non-Hodgkin lymphoma(NHL) in CR1/CR2 and for patients with chronic myeloid leukemia in the first and second chronic phase lacking a matched donor, we designed a propensity score method-based multicenter study.Hematopoietic recovery, acute graft-versus-host disease(aGVHD), and chronic GVHD were comparable between the HBMT group(n=168) and the HPBSCT group(n=42). No significant differences were found in non-relapse mortality rate(20.17%±3.58%and 27.24%±7.16%, P=0.18) or relapse rate(19.96%±3.72% and 28.49%±8.25%, P=0.32) between the HBMT group and the HPBSCT group. HBMT recipients had better overall survival(65.0%±4.2% and 54.2%±8.3%, P=0.037) and disease-free survival(59.9%±4.6% and 44.3%±8.7%, P=0.051). Multivariate analysis showed that HPBSCT was associated with poorer DFS(HR(95%CI), 1.639(0.995–2.699), P=0.052). Our comparisons showed that HBMT was superior to HPBSCT as a post-remission treatment for patients lacking an identical donor.

关键词： HBMT GPB Beijing Protocol HSCT

Gut microbiome alterations and its link to corticosteroid resistance in immune thrombocytopenia

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Science China(Life Sciences) 2021年第5期64卷 766-783页

作者： Yanan Wang Fengqi Liu Gaochao Zhang Yan Su Xueyan Sun Qi Chen Chencong Wang Haixia Fu Yun He Xiaolu Zhu Xiao Liu Meng Lv Xiangyu zhao xiaosu zhao Yueying Li Qianfei Wang Xiaojun Huang Xiaohui Zhang Peking University People's Hospital Peking University Institute of HematologyBeijing Key Laboratory of Hematopoietic Stem Cell TransplantationBeijing 100044China Collaborative Innovation Centre of Hematology Peking UniversityBeijing 100044China National Clinical Research Center for Hematologic Disease Beijing 100044China CAS Key Laboratory of Genomic and Precision Medicine Collaborative Innovation Center of Genetics and DevelopmentBeijing Institute of GenomicsChinese Academy of SciencesBeijing 100101China National Center for Bioinformation Beijing 100101China University of Chinese Academy of Sciences Beijing 100049China

Quantitative metagenomic studies have linked the gut microbiota to autoimmune disorders.Here,we performed deep shotgun metagenomic sequencing of fecal samples from 99 immune thrombocytopenia(ITP)patients and 52 healthy controls.Dysbiosis in the gut microbiome of ITP was detected phylogenetically and functionally,and classifier based on species markers distinguished individuals with ITP from healthy controls.In particular,the abundance of Ruminococcus gnavus,Bifidobacterium longum and Akkermansia muciniphila was markedly increased in treatment-na?ve ITP patients,and the alterations of microbial species were correlated with clinical indices.Functionally,the secondary bile acid biosynthesis and flagellar assembly were depleted in the gut microbiota of ITP,which may contribute to the onset of ITP by affecting the immune system.Furthermore,we found that corticosteroid treatment affected the gut microbiome of ITP.Compared with corticosteroid-sensitive ITP patients,we identified that the corticosteroid-resistant ITP patients displayed a distinct gut microbiome,which was different from that of the treatment-na?ve ITP patients.Together,we provided support for the critical role of gut microbiota in the development of ITP and established a foundation for further research characterizing gut microbiota in relation to corticosteroid resistance of ITP.

关键词： immune thrombocytopenia metagenomics gut microbiome corticosteroid resistance

The past and future of USTC: an interview with USTC President Xinhe Bao

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National Science Review 2019年第3期6卷 601-605页

作者： Weijie zhao xiaosu Yi NSR

The University of Science and Technology of China (USTC) is located in Hefei, the capital of Anhui province, and has its own characteristics among the universities in China. Established by the Chinese Academy of Sciences (CAS), USTC is distinctively tinted with a scientific color.It is also famous for its ‘Special Class for the Gifted Young’ and is considered one of the best Chinese universities in the fields of science and technology (S&T). Recently, National Science Review interviewed Professor Xinhe Bao, the President of USTC, about the characteristics

关键词： China an interview with USTC President Xinhe Bao The past and future of USTC

人机协作对项目团队绩效的影响研究:有调节的中介效应模型

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土木工程与管理学报 2024年第3期41卷 80-86,94页

作者：石世英赵方方彭新新张净净叶晓甦河南理工大学土木工程学院河南焦作454003 重庆大学管理科学与房地产学院重庆400044

鉴于人机协作对员工及团队绩效的影响机制存在认知差异,整合资源保存理论、自我调节理论以及角色理论,构建人机协作、团队绩效,团队创造力以及团队虚拟性的被调节中介模型,并结合模糊认知图(FCM)仿真方法,对影响因素之间的相关性进行动... 详细信息

鉴于人机协作对员工及团队绩效的影响机制存在认知差异,整合资源保存理论、自我调节理论以及角色理论,构建人机协作、团队绩效,团队创造力以及团队虚拟性的被调节中介模型,并结合模糊认知图(FCM)仿真方法,对影响因素之间的相关性进行动态分析。研究结果表明:(1)人机协作对团队绩效具有显著正向影响;团队创造力在人机协作与团队绩效关系之间发挥显著的中介作用;(2)团队虚拟性在直接效应和中介效应中均发挥了调节作用;(3)通过情景分析证明人机协作是影响团队绩效的最根本原因,团队虚拟性是智能技术应用的边界条件。研究结论拓展了项目情境下人机协作在项目领域的影响效应和团队虚拟性的调节机制,为项目团队科学合理应用智能技术和团队绩效管理提供借鉴。

关键词：人机协作工程项目团队绩效团队虚拟性团队创造力

西罗莫司联合抗CD20单克隆抗体去敏治疗对单倍型相合造血干细胞移植患者预后的影响

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中华医学杂志 2024年第11期104卷 843-849页

作者：马宁王志东孙于谦闫晨华王峰蓉莫晓东吕萌赵翔宇赵晓甦韩伟陈欢陈育红王昱许兰平程翼飞张晓辉刘开彦黄晓军常英军北京大学人民医院血液科北京大学血液病研究所国家血液系统疾病临床医学研究中心造血干细胞移植治疗血液病北京市重点实验室北京100044

目的探讨西罗莫司联合抗CD20单克隆抗体去敏治疗对单倍型相合造血干细胞移植(haplo-SCT)患者预后的影响。方法回顾性纳入2021年11月至2023年3月在北京大学血液病研究所接受haplo-SCT、移植前特异性抗人类白细胞抗原(HLA)抗体(DSA)阳性[... 详细信息

目的探讨西罗莫司联合抗CD20单克隆抗体去敏治疗对单倍型相合造血干细胞移植(haplo-SCT)患者预后的影响。方法回顾性纳入2021年11月至2023年3月在北京大学血液病研究所接受haplo-SCT、移植前特异性抗人类白细胞抗原(HLA)抗体(DSA)阳性[平均荧光强度(MFI)≥2000]的连续血液病患者15例作为去敏治疗组,其中男4例,女11例,年龄[M(Q_(1),Q_(3))]为48(37,59)岁;所有患者均接受西罗莫司联合抗CD20单克隆抗体进行去敏治疗。未去敏治疗组为2012年8月至2016年6月未接受去敏治疗的29例haplo-SCT患者,男12例,女17例,年龄为42(26,50)岁。随访截至2023年10月1日,去敏治疗组中位随访时间为13(9,18)个月,未去敏治疗组中位随访时间为23(14,29)个月。比较去敏治疗组患者去敏治疗前后MFI变化情况,以及两组患者预后情况,包括原发性植入失败(pGF)发生率、中性粒细胞植入时间、血小板植入时间、Ⅱ~Ⅳ度急性移植物抗宿主病(GVHD)及慢性GVHD发生率、非复发死亡率、无事件生存率、无病生存率和总生存率的差异;采用Kaplan-Meier法绘制生存曲线,组间生存率比较采用log-rank检验。结果去敏治疗组患者接受去敏治疗后,DSA MFI水平[M(Q_(1),Q_(3))]从8879(7544,11495)降低至3781(1638,4165)(P0.05)。去敏治疗组预期1年无事件生存率为100%(15/15),高于未去敏治疗组的51.3%(15/29)(P=0.002)。结论西罗莫司联合抗CD20单克隆抗体去敏治疗可降低haplo-SCT受者DSA水平,促进移植后造血植入,避免移植后pGF的发生。

关键词：造血干细胞移植抗CD20单克隆抗体西罗莫司人类白细胞抗原抗体

Applied computer aided system for hotplate butt welding of plastics

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China Welding 1995年第2期4卷 127-131页

作者： zhao Gaoming and Yi xiaosu(Zhejiang University. Hangzhou)

This paper illustrates the construction and application of the applied computeraided .system for hotplate butt welding of plastics with regard to inquiry, imitation and Optimization.

关键词： plastics butt welding. computer aided system

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急性白血病微小残留病概念的内涵和外延

白血病．淋巴瘤 2023年第1期32卷 8-11页

作者：常英军赵晓甦北京大学人民医院血液科、北京大学血液病研究所、国家血液系统疾病临床医学研究中心、造血干细胞移植治疗血液病北京市重点实验室北京100044

微小残留病(MRD)不仅被用于急性白血病的复发预测、预后再分层和指导抢先干预,还被用于其他恶性血液病和实体肿瘤的治疗领域。深入认识MRD的内涵和外延对于探索精准预测复发新方法、完善恶性肿瘤个体化干预策略等均具有重要意义。

关键词：白血病,急性微小残留病恶性血液病恶性肿瘤个体化治疗