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Correlation of gut microorganisms and non-volatile flavor substances provides new insights for breeding Scylla paramamosain

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Journal of Oceanology and Limnology 2024年第4期42卷 1336-1347页

作者： Gao GAO Xing LI Kangxiang QIN Yun HU xiaosong jiang Chenxi CHE Yuntao LI Changkao MU Chunlin WANG Huan WANG School of Marine Science Ningbo UniversityNingbo 315211China Key Laboratory of Applied Marine Biotechnology Ministry of EducationNingbo UniversityNingbo 315211China

The farming of Scylla paramamosain with specific flavors has a higher commercial value,and the flavors are related to the integrated farming environment and non-volatile flavor substances,while the survival environment is one of the important ways to source gut microorganisms in the *** this study,the levels of dominant taxa in the gut flora of *** from Mong Cai,Vietnam(VN),Taishan City,Guangdong Province(TS)of China,and Ninghai County,Ningbo City(NB)Zhejiang Province of China converged with those of *** from Sanmen County,Ningbo City(CK 1,CK 2,and CK 3)at 28 d of *** top 15 genera with the highest abundance of VN,TS,and NB gut flora were the same as CK 1,CK 2,and CK 3,but with different percentages,and gradually converged to CK 1,CK 2,and CK 3,respectively,at 28 d of *** between intestinal flora and non-volatile flavor substances in the hepatopancreas at the percentage level of relative abundance of bacterial genera found that above 28 d of domestication,Muribaculaceae,Psychrilyobacter,Clostridia_vadinBB 60_group,Halarcobacter Carboxylicivirga,Sediminispirochaeta may be the most important genera affecting flavor amino acids of ***,Carboxylicivirga,Halarcobacter,Photobacterium,ZOR 0006,Psychrilyobacter,and Pseudomonas may be the most important genera affecting flavor amino acids of ***,Carboxylicivirga,Halarcobacter,Photobacterium,ZOR 0006,Vibrio,and Sphingomonas may be the most important genera affecting flavor amino acids of *** results show that the intestinal flora structure of crabs from different areas were domesticated in the same area for at least 28 d before they converged to that of the domesticated crab,and the most important genera affecting the flavor amino acids of TS,VN,and NB were also *** results of this study provide a reference and basis for the technique of directional cultivation of the flavor quality of the crab.

关键词： survival environment Scylla paramamosain intestinal flora structure non-volatile flavor substance directional breeding

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基于多业务通信终端检测的短波通信技术研究

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通信电源技术 2024年第6期41卷 173-175页

作者：蒋小松常州国光数据通信有限公司江苏常州213000

基于多业务通信终端检测研究一种新的短波通信技术。根据数据业务、语音业务及手键报业务功能对多业务通信终端进行分类,全面了解不同业务对通信终端的要求。设计短波通信网络拓扑结构,提出设计目标与约束条件,根据设计结果进行终端部署... 详细信息

基于多业务通信终端检测研究一种新的短波通信技术。根据数据业务、语音业务及手键报业务功能对多业务通信终端进行分类,全面了解不同业务对通信终端的要求。设计短波通信网络拓扑结构,提出设计目标与约束条件,根据设计结果进行终端部署,并讨论网络节点、连接方式以及通信信道的分配与管理策略,以满足不同应用场景的需求。从信号分类、频率和频谱分析以及调制与解调等方面,提高通信终端对短波信号的识别和处理能力。通过实验研究验证了所提出技术的有效性和可行性。

关键词：多业务通信通信终端终端检测短波通信

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应急广播监管系统设计与思考

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电视技术 2024年第8期48卷 86-88,95页

作者：蒋晓宋湖南广播电视网络传输中心湖南长沙410003

针对应急广播体系建设中效果监测评估系统规范不统一、监测内容有限的实际情况,结合行业的监管需求,介绍应急广播监管系统的设计与实现方法,为行业提供借鉴与参考。

关键词：应急广播监管镜像抓包

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“Find Me”and“Eat Me”signals:tools to drive phagocytic processes for modulating antitumor immunity

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Cancer Communications 2024年第7期44卷 791-832页

作者： Lingjun Xiao Louqian Zhang Ciliang Guo Qilei Xin xiaosong Gu Chunping jiang Junhua Wu State Key Laboratory of Pharmaceutical Biotechnology National Institute of Healthcare Data Science at Nanjing UniversityJiangsu Key Laboratory of Molecular MedicineMedical SchoolNanjing UniversityNanjingJiangsuP.R.China Jinan Microecological Biomedicine Shandong Laboratory JinanShandongP.R.China

Phagocytosis,a vital defense mechanism,involves the recognition and elimination of foreign substances by ***,such as neutrophils and macrophages,rapidly respond to invaders;macrophages are especially important in later stages of the immune *** detect“find me”signals to locate apoptotic cells and migrate toward *** cells then send“eat me”signals that are recognized by phagocytes via specific receptors.“Find me”and“eat me”signals can be strategically harnessed to modulate antitumor immunity in support of cancer *** signals,such as calreticulin and phosphatidylserine,mediate potent pro-phagocytic effects,thereby promoting the engulfment of dying cells or their remnants by macrophages,neutrophils,and dendritic cells and inducing tumor cell *** review summarizes the phagocytic“find me”and“eat me”signals,including their concepts,signaling mechanisms,involved ligands,and ***,we delineate the relationships between“find me”and“eat me”signaling molecules and tumors,especially the roles of these molecules in tumor initiation,progression,diagnosis,and patient *** interplay of these signals with tumor biology is elucidated,and specific approaches to modulate“find me”and“eat me”signals and enhance antitumor immunity are ***,novel therapeutic strategies that combine“find me”and“eat me”signals to better bridge innate and adaptive immunity in the treatment of cancer patients are discussed.

关键词： cancer immunotherapy CARL CX3CL1 “Eat me”signal “Find me”signal Fc LPC Phagocytosis PtSer SLAMF7

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Long-term functional maintenance of primary hepatocytes in vitro using macroporous hydrogels engineered through liquid-liquid phase separation

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Nano Research 2024年第3期17卷 1725-1736页

作者： Yang Sun Sheng Yin Jian Cui Zhongxia Wang Yueying Han Ding Ma Shuo Wang Junhua Wu Yi Cao Chunping jiang xiaosong Gu Jinan Microecological Biomedicine Shandong Laboratory Jinan 250021China Collaborative Innovation Center of Advanced Microstructures National Laboratory of Solid State MicrostructureKey Laboratory of Intelligent Optical Sensing and ManipulationMinistry of EducationDepartment of PhysicsNanjing UniversityNanjing 210093China Department of Hepatobiliary Surgery The Affiliated Drum Tower Hospital of Nanjing University Medical SchoolNanjing 210093China National Institute of Healthcare Data Science at Nanjing University Nanjing UniversityNanjing 210093China

Preserving the functionality of hepatocytes in vitro poses a significant challenge in liver tissue engineering and bioartificial liver,as these cells rapidly lose their metabolic and functional characteristics after *** by the macroporous structures found in native liver tissues,here we develop synthetic hydrogel scaffolds that closely mimic the liver’s structural organization through the phase separation between polyethylene glycol(PEG)and *** hydrogels exhibit interconnected macroporous structures and appropriate mechanical properties,providing an optimal microenvironment conducive to hepatocyte adhesion and the formation of sizable *** to two-dimensional hepatocyte cultures,enhanced functionalities of hepatocytes cultured in our macroporous hydrogels were observed for 14 days,as evidenced by quantitative reverse-transcription–polymerase chain reactions(qRT-PCR),immunofluorescence,and enzyme linked immunosorbent assay(ELISA)*** sequencing data further confirmed the establishment of cell–cell interactions among hepatocytes when cultured in our ***,these hepatocytes maintained a protein expression lineage that closely resembled freshly isolated hepatocytes,particularly in the Notch and tumor necrosis factor(TNF)signaling *** results suggest that the macroporous hydrogels are attractive scaffolds for liver tissue engineering.

关键词： liver tissue engineering phase separation hepatocytes macroporous hydrogel

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miRNA sequencing analysis of healthy and atretic follicles of chickens revealed that miR-30a-5p inhibits granulosa cell death via targeting Beclin1

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Journal of Animal Science and Biotechnology 2022年第5期13卷 1351-1372页

作者： Haorong He Dongmei Li Yongtong Tian Qinyao Wei Felix Kwame Amevor Congjiao Sun Chunlin Yu Chaowu Yang Huarui Du xiaosong jiang Menggen Ma Can Cui Zhichao Zhang Kai Tian Yao Zhang Qing Zhu Huadong Yin Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province Sichuan Agricultural UniversityChengdu 611130SichuanChina National Engineering Laboratory for Animal Breeding and Key Laboratory of Animal Genetics Breeding and ReproductionMinistry of Agriculture and Rural AffairsCollege of Animal Science and TechnologyChina Agricultural UniversityBeijing 100193China Animal Breeding and Genetics key Laboratory of Sichuan Province Sichuan Animal Science AcademyChengdu 610066China College of Resources Sichuan Agricultural UniversityChengdu 611130China

Background:The egg production performance of chickens is affected by many factors,including genetics,nutrition and environmental *** factors all play a role in egg production by affecting the development of ***(miRNAs)are important non-coding RNAs that regulate biological processes by targeting genes or other non-coding RNAs after *** the animal reproduction process,miRNA is known to affect the development and atresia of follicles by regulating apoptosis and autophagy of granulosa cells(GCs).Results:In this study,we identified potential miRNAs in the atretic follicles of broody chickens and unatretic follicles of healthy *** identified gga-miR-30a-5p in 50 differentially expressed miRNAs and found that gga-miR-30a-5p played a regulatory role in the development of chicken *** function of miR-30a-5p was explored through the transfection test of miR-30a-5p inhibitor and miR-30a-5p *** the study,we used qPCR,western blot and flow cytometry to detect granulosa cell apoptosis,autophagy and steroid hormone *** microscopy and transmission electron microscopy are used for the observation of *** levels of estradiol(E2),progesterone(P4),malondialdehyde(MDA)and superoxide dismutase(SOD)were detected by *** results showed that miR-30a-5p showed a negative effect on autophagy and apoptosis of granulosa cells,and also contributed in steroid hormones and reactive oxygen species(ROS)*** addition,the results obtained from the biosynthesis and dual luciferase experiments showed that Beclin1 was the target gene of *** rescue experiment conducted further confirmed that Beclin1 belongs to the miR-30a-5p regulatory ***:In summary,after deep miRNA sequencing on healthy and atretic follicles,the results indicated that miR-30a-5p inhibits granulosa cell death by inhibiting Beclin1.

关键词： Apoptosis Autophagy Beclin1 Chicken granulosa cells MiR-30a-5p Oxidative stress RNA-seq Steroid hormone

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省级有线数字电视监管平台系统设计

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广播与电视技术 2008年第9期35卷 98-98,100,101页

作者：蒋晓宋湖南广播电视网络传输中心

本文根据湖南省有线数字电视监管平台的建设实践,描述了省级有线数字电视监管平台一些新的设计思路,结合职能部门对监管平台的技术需求,介绍了监管平台的主要功能设计和实现方法。

关键词：监管 ES流码流比对质量服务

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大恒799肉鸡Myoz1基因CDs区克隆、序列分析及其组织表达研究

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中国家禽 2024年第9期46卷 26-33页

作者：朱师良余春林邱莫寒张增荣熊霞胡陈明杨礼彭涵陈家磊宋小燕夏波刘思洋蒋小松杜华锐李晴云杨朝武四川省畜牧科学研究院动物遗传育种重点实验室四川成都610066 四川大恒家禽育种有限公司四川成都610066

为研究钙调神经磷酸酶肌小结结合蛋白1(Myoz1)的氨基酸序列特征及其在不同组织器官中的mRNA表达水平,试验通过PCR克隆Myoz1 CDs序列,分别利用ExPASYProt⁃param、ProtScale、Target P、DNAStar Protean、SWISS-MODEL、SignalP 4.1 Server、NetPhos2.0Serv⁃er和STRTNG软件,进行Myoz1蛋白理化特性、亲/疏水性、亚细胞结构、蛋白二级结构、三级结构、信号肽、磷酸化位点和蛋白互作分析,并通过实时荧光定量PCR(qRT-PCR)检测Myoz1基因表达量。结果显示:大恒799肉鸡Myoz1基因CDs序列全长为881 bp,可编码293个氨基酸;大恒799肉鸡Myoz1基因氨基酸序列与雉鸡、岩雷鸟位于一个分支,同源性最高,均高于96%,亲缘关系最近;Myoz1蛋白为水溶性蛋白且大多分布于细胞核内,分子式为C1442H_(2)244N400O430S10,分子质量约为32384.70 ku,理论等电点为9.12;Myoz1蛋白不存在信号肽,为分泌蛋白,共存在37个潜在的磷酸化位点、1个N-糖基化潜在位点;Myoz1蛋白二级结构主要由α-螺旋、β-转角和无规则卷曲组成,三级结构预测结果与其一致;Myoz1蛋白与ACTN2、LDB3、LMOD3等Z盘蛋白(PDZ-LIM结构域蛋白,ZASP)以及肌肉生长发育相关蛋白有相互作用的关系;Myoz1在1日龄大恒799肉鸡胸肌和腿肌的表达量显著高于其他组织,且在胸肌中的表达量高于腿肌(P<0.05)。研究表明Myoz1基因可能在肌肉生长发育过程中发挥调控作用。

关键词：大恒肉鸡 Myoz1基因克隆生物信息学分析组织表达

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综合通信系统检测控制平台的设计

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计算机工程 2005年第10期31卷 225-227页

作者：陈德煌蒋晓松牛强军空军第一航空学院信阳464000

机载综合通信系统各组成设备的地面维修检查与测试,需要一个以仿真总线控制器为核心的检测控制平台。文章介绍了该平台的设计思想、硬件和软件组成及原理,给出了基于计算机的仿真总线控制器的设计方法。

关键词：综合系统总线控制器航空通信检测

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Research Progress on Effects of Stocking Density on Performance, Health and Welfare of Duck

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Agricultural Biotechnology 2020年第4期9卷 111-114,119页

作者： Chaowu YANG Chunlin YU Zengrong ZHANG Mohan QIU Huarui DU Qingyun LI xiaosong jiang Xia XIONG Animal Breeding and Genetics key Laboratory of Sichuan Province Sichuan Animal Science AcademyChengdu 610066China

Stocking density is an important factor for animal production *** appears particularly urgent and important to research systematically on stocking density for the dual restriction of environmental protection and animal *** review dealt with the influence of stocking density on ducks'productivity and health through production performance,animal behavior and animal welfare,and analyzed the possible mechanism of high stocking density reducing production performance,resulting in abnormal behavior and stress,causing welfare and health *** consider that it might be associated with heat stress,more spatial competition(to gather the food,drinking water,sports area,etc.),as well as the quality deterioration of litter and house air caused by high stocking ***,we put forward some suggestions on the study of stocking density's effects on ducks,and proposed the future work that need to be studied and problems that need to be solved imminently.

关键词： Duck Production Stocking Density Animal Welfare Performance

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