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草地早熟禾苗期耐低温的生理特性研究

中国草地学报 2024年第5期46卷 33-43页

作者：董文科张玉娟马福钦陈佐惠周晓爽张蓓张佳能甘肃农业大学草业学院/草业生态系统教育部重点实验室/甘肃省草业工程实验室/中-美草地畜牧业可持续发展研究中心/农业农村部饲草种质创新与新品种选育重点实验室甘肃兰州730070

为了探究不同抗性草地早熟禾苗期对低温胁迫的生理特性差异,以10个常用草地早熟禾品种为试验材料,将幼苗叶片进行不同程度的低温胁迫处理,利用细胞伤害率和对应的低温度梯度进行Logistic方程拟合,获得半致死温度(LT50)并筛选抗寒品种和... 详细信息

为了探究不同抗性草地早熟禾苗期对低温胁迫的生理特性差异,以10个常用草地早熟禾品种为试验材料,将幼苗叶片进行不同程度的低温胁迫处理,利用细胞伤害率和对应的低温度梯度进行Logistic方程拟合,获得半致死温度(LT50)并筛选抗寒品种和低温敏感品种,并以二者为试验材料进行相关生理指标的测定和相关性分析。结果表明,草地早熟禾‘普瑞芬’的LT_(50)为-17.5℃,表现出较好的抗寒性,‘帝国’的LT_(50)为-3.2℃,表现出对低温的敏感性;经低温胁迫处理后抗寒品种‘普瑞芬’的相对电导率和丙二醛含量显著下降并低于低温敏感品种‘帝国’,而‘普瑞芬’幼苗中干物质含量、相对含水量和苗长等生长指标和叶绿素含量、渗透调节物质(游离脯氨酸、可溶性糖和可溶性蛋白)和抗氧化酶活性(SOD、POD和CAT)均高于‘帝国’;经相关性分析表明,低温对草地早熟禾幼苗的干物质,苗长和根长及渗透调节物质含量影响较大且各指标之间相互作用。

关键词：草地早熟禾低温胁迫半致死温度生理特性相关性

丹酚酸B通过调控AMPK介导的铁死亡保护缺氧/复氧诱导的肾小管上皮细胞的损伤

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海南医学院学报 2024年第15期30卷 1147-1155页

作者：赵静宇陈发慧郝亚杰韩秀涛任茜雅田瑞雪周晓霜山西中医药大学第三临床学院山西晋中030619 山西医科大学第五临床医学院肾内科山西太原030012 山西省人民医院肾内科山西太原030012

目的:探究丹酚酸B(Salvianolic acid B,Sal B)对于缺氧/复氧(hypoxia/reoxygenation,H/R)所致肾小管上皮细胞损伤的保护作用及其机制。方法:体外实验细胞分组为正常对照(Con)组,Sal B+Con组、H/R组、Sal B+H/R组,以及Con组、H/R组、Sal B+H/R组、H/R+CC(Compound c,AMPK抑制剂)组、H/R+Sal B+CC组两部分。体外实验用CCK‑8法检测HK‑2细胞活性;细胞震碎,取上清,用酶标仪检测GSH和MDA的含量;Western blot检测p‑AMPK、GPX4、ACSL4和FSP1蛋白的表达情况;细胞免疫荧光检测铁死亡指标GPX4、ACSL4蛋白的表达情况;流式细胞术检测线粒体膜电位和细胞凋亡。结果:体外实验结果显示,Sal B在20~160μmol/L对正常HK‑2细胞活性无明显毒性(P>0.05);与模型组相比,20、40、80μmol/L的Sal B呈剂量依赖性提高HK‑2细胞的活性(P<0.01)。与模型组相比,H/R+Sal B组细胞MDA含量降低,GSH含量升高,p‑AMPK、GPX4和FSP1蛋白的表达水平升高,ACSL4蛋白的表达降低,细胞线粒体膜电位显著升高,细胞凋亡率显著下降(P<0.05);H/R+AMPK组较H/R+Sal B组FSP1和GPX4蛋白表达水平降低,ACSL4蛋白的表达水平升高(P<0.05),细胞线粒体膜电位显著降低(P<0.05),加入Sal B可以逆转这一情况(P<0.05)。结论:Sal B可减轻H/R的肾小管上皮细胞的损伤,其机制可能与激活AMPK通路减轻肾小管上皮细胞铁死亡有关。

关键词：急性肾损伤丹酚酸B 铁死亡 AMPK 缺氧/复氧

PD-1 Involvement in Peripheral Blood CD8+T Lymphocyte Dysfunction in Patients with Acute-on-chronic Liver Failure

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Journal of Clinical and Translational Hepatology 2021年第3期9卷 283-290页

作者： xiaoshuang zhou Yidong Li Yaqiu Ji Tian Liu Ninghui Zhao Jiefeng He Jia Yao Department of Nephrology Shanxi Provincial People’s HospitalShanxi Medical UniversityTaiyuanShanxiChina Department of Gastroenterology Shanxi Bethune HospitalShanxi Medical UniversityTaiyuanShanxiChina Department of Hepatobiliary Surgery Shanxi Bethune HospitalShanxi Medical UniversityTaiyuanShanxiChina Institute of Liver Disease and Organ Transplantation Shanxi Medical UniversityTaiyuanShanxiChina

Background and Aims:Programmed cell death-1(PD-1)plays an important role in downregulating T lymphocytes but the mechanisms are still poorly *** study aimed to explore the role of PD-1 in CD8^(+)T lymphocyte dysfunction in hepatitis B virus(HBV)-related acute-on-chronic liver failure(ACLF).Methods:Thirty patients with HBV-ACLF and 30 healthy controls(HCs)were *** differences in the numbers and functions of CD8^(+)T lymphocytes,PD-1 and glucose transporter-1(Glut1)expression from the peripheral blood of patients with HBV-ACLF and HCs were *** vitro,the CD8^(+)T lymphocytes from HCs were cultured(HC group)and the CD8^(+)T lymphocytes from ACLF patients were cultured with PD-L1-IgG(ACLF+PD-1 group)or IgG(ACLF group).The numbers and functions of CD8^(+)T lymphocytes,PD-1 expression,glycogen uptake capacity,and Glut1,hexokinase-2(HK2),and pyruvate kinase(PKM2)expression were analyzed among the HC group,ACLF group and ACLF+***:The absolute numbers of CD8^(+)T lymphocytes in the peripheral blood from patients with HBVACLF were lower than in the HCs(p<0.001).The expression of PD-1 in peripheral blood CD8^(+)T lymphocytes was lower in HCs than in patients with HBV-ACLF(p=0.021).Compared with HCs,PD-1 expression was increased(p=0.021)and Glut1 expression was decreased(p=0.016)in CD8^(+)T lymphocytes from the HBV-ACLF *** vitro,glycogen uptake and functions of ACLF CD8^(+)T lymphocytes were significantly lower than that in HCs(p=0.017;all p<0.001).When PD-1/PD-L1 was activated,the glycogen uptake rate and expression levels of Glut1,HK2,and PKM2 showed a decreasing trend(ACLF+PD-1 group compared to ACLF group,all p<0.05).The functions of CD8^(+)T lymphocytes in the ACLF+PD-1 group[using biomarkers of Ki67,CD69,IL-2,interferon-gamma,and tumor necrosis factor-alpha-were lower than in the ACLF group(all p<0.05).Conclusions:CD8^(+)T lymphocyte dysfunction is observed in patients with ***-1-induced T lymphocyte dysfunction mig

关键词： Acute and chronic liver failure Programmed cell death 1 Immune function Glycolysis Glut1

Peptide drugs: a new direction in cancer immunotherapy

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Cancer Biology & Medicine 2024年第3期21卷 198-203页

作者： Xinghua Sui xiaoshuang Niu Xiuman zhou Yanfeng Gao School of Pharmaceutical Sciences(Shenzhen) Shenzhen Campus of Sun Yat-sen UniversityShenzhen 518107China

Cancer immunotherapy has emerged as a promising approach in cancer treatment and is considered a major advancement after surgical interventions, radiotherapy, chemotherapy, and targeted therapy. The clinical use of im... 详细信息

关键词： drugs immunotherapy cancer

MicroRNA-21 in the pathogenesis of acute kidney injury

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Protein & Cell 2013年第11期4卷 813-819页

作者： Ya-Feng Li Ying Jing Jielu Hao Nathan C Frankfort xiaoshuang zhou Bing Shen Xinyan Liu Lihua Wang Rongshan Li Department of Nephrology and Hemodialysis Center Second Hospital of Shanxi Medical UniversityTaiyuan 030001China Shanxi Renal Disease Research Institution Taiyuan 030001China Department of Blood Purifi cation General Hospital of Jinan Military Command of PLAJinan 250031China Nephrology Institute of PLA Changzheng Hospital of Second Military Medical UniversityShanghai 200003China Department of Neuroscience and Neurovirology Temple University School of MedicinePhiladelphiaPA 19140USA Department of Urology Shanghai First People’s HospitalShanghai 200080China Department of Nephrology and Hemodialysis Center Shanxi Provincial People’s HospitalTaiyuan 030012China

Acute kidney injury(AKI),associated with significant mor-bidity and mortality,is widely known to involve epithelial apoptosis,excessive inflammation,and fibrosis in re-sponse to ischemia or reperfusion injury,which results in either chronic pathological changes or ***,it is imperative that investigations are conducted in order to fi nd effective,early diagnoses,and therapeutic targets needed to help prevent and treat ***,the mech-anisms modulating the pathogenesis of AKI still remain largely ***(miRNAs),small non-coding RNA molecules,play an important role in several fundamental biological and pathological processes by a post transcriptional regulatory function of gene ***-21(miR-21)is a recently identifi ed,typi-cal miRNA that is functional as a regulator known to be involved in apoptosis as well as inflammatory and fi brotic signaling pathways in *** a result,miR-21 is now considered a novel biomarker when diagnosing and treat-ing *** article reviews the correlative literature and research progress regarding the roles of miR-21 in AKI.

关键词： microRNA microRNA-21 gene expression acute kidney injury

Gender differences in the relationship between plasma lipids and fasting plasma glucose in non-diabetic urban Chinese popula- tion： a cross-section study

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Frontiers of Medicine 2014年第4期8卷 477-483页

作者： Jie Zheng Yuzhen Gao Yuejuan Jing xiaoshuang zhou Yuanyuan Shi Yanhong Li Lihua Wang Ruiying Wang Maolian Li Chuanshi Xiao Yafeng Li Rongshan Li School of Nursing Shanxi Medical University Taiyuan 030001 China Department of Cardiology Second Hospital of Shanxi Medical University Taiyuan 030001 China Department of Nephrology and Hemodialysis Center Second Hospital of Shanxi Medical University Taiyuan 030001 China Shanxi Renal Disease Research Institution Taiyuan 030001 China Department of Cardiology First Hospital of Shanxi Medical University Taiyuan 030001 China Department of Nephrology and Hemodialysis Center Shanxi Provincial People's Hospital Taiyuan 030012 China

The association between dyslipidemia and elevated fasting glucose in type 2 diabetes is well known. In non-diabetes, whether this association still exists, and whether dyslipidemia is an independent risk factor for high fasting plasma glucose （FPG） levels are not clear. This cross-sectional study recruited 3460 non-diabetic Chinese subjects （1027 men, and 2433 women, aged 35-75 years old） who participated in a health survey. Men and women were classified into tertiles by levels of plasma lipids respectively. In women, the prevalence of impaired fasting glucose （IFG） was decreased with increased HDL-C. A stepwise increase in HDL-C was associated with decreasing FPG levels （lowest tertiles, FPG： 5.376 ± 0.018; middle tertiles, 5.324± 0.018; highest tertiles, 5.276±0.018mmol/L; P = 0.001）. Reversely, FPG levels increased from lowest tertiles to highest tertiles of LDL-C, TC, and TG. we found that women in the first tertile with lower HDL-C level had a 1.75-fold increase in risk of IFG compared with non-diabetic women in the third tertile with higher HDL-C level （OR： 1.75; 95% CI： 1.20-2.56）. In men, no significant association was found. We took age, BMI, waist/hip ratio, education, smoking, alcohol drinking, and physical exercise as adjusted variables. In Chinese non-diabetic women, dyslipidemia is independently associated with high levels of FPG; TG, HDL-C, and LDL-C are predictors of IFG independent of BMI and waist/hip ratio.

关键词： dyslipidemia plasma lipids plasma fasting glucose impaired fasting glucose non-diabetes

Plasmon Bi in-situ anchored on BiOCl nanosheets assembled microspheres towards optimized photothermal-photocatalytic performance

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Chinese Chemical Letters 2024年第2期35卷 469-476页

作者： Dongxue Song Mingxia Li Fan Yang Menghan Yu Zhenzi Li Jie Chen xiaoshuang Zhang Wei zhou School of Chemistry and Materials Science Key Laboratory of Functional Inorganic Material ChemistryMinistry of Education of the People's Republic of ChinaHeilongjiang UniversityHarbin 150080China Shandong Provincial Key Laboratory of Molecular Engineering School of Chemistry and Chemical EngineeringQilu University of Technology (Shandong Academy of Sciences)Ji'nan 250353China

Interface engineering is of great importance to improve the photocatalytic ***,in-situ formation plasmon Bi/BiOCl nanosheets assembled heterojunction microspheres are fabricated via facile reductive solvothermal *** aldehyde group in the DMF structure is used to exert the weak reducing property of the solvent and thus strip out the metal Bi in *** metal Bi is anchored on surface of BiOCl firmly due to in-situ formation engineered interface,which could realize efficient charge transfer *** resultant Bi/BiOCl heterojunctions assemblies with narrow bandgap of 3.05 eV and mesoporous structure extend the photoresponse to visible light region and could provide sufficient surface active *** visible-light-driven photocatalytic degradation of high-toxic norfloxacin for Bi/BiOCl heterojunctions is up to 95.5%within 20 min,representing several times that of pristine BiOCl nanosheets and the physical *** is attributed to the in-situ formation of Bi/BiOCl heterojunctions and surface plasmon resonance(SPR)effect of plasmon Bi promoting charge transfer,and the obvious photothermal effect promoting the photocatalytic reaction,which are verified by experimental and density functional theory(DFT)*** strategy provides ideal perspectives for fabricating metal/semiconductor heterojunctions photocatalysts with high-performance.

关键词： Photocatalysis BiOCl Heterojunction Plasmon Bi Mesoporous structure

Increased dopamine and its receptor dopamine receptor D1 promote tumor growth in human hepatocellular carcinoma

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Cancer Communications 2020年第12期40卷 694-710页

作者： Yan Yan Jiahao Pan Yonghua Chen Wei Xing Qiang Li Dongyin Wang xiaoshuang zhou Jingdun Xie Changhong Miao Yunfei Yuan Weian Zeng Dongtai Chen Department of Anesthesiology Sun Yat-Sen University Cancer CenterState Key Laboratory of Oncology in South ChinaCollaborative Innovation Center for Cancer MedicineGuangzhouGuangdong 510060P.R.China Department of Anesthesiology Huizhou Municipal Central HospitalHuizhouGuangdong 516001P.R.China Department of Anesthesiology Peking University Shenzhen HospitalShenzhenGuangdong 518000P.R.China Department of Anesthesiology Fudan University Zhongshan HospitalShanghai 20032P.R.China Department of Hepatobiliary Oncology Sun Yat-Sen University Cancer CenterState Key Laboratory of Oncology in South ChinaCollaborative Innovation Center for Cancer MedicineGuangzhouGuangdong 510060P.R.China

Background:Dopamine and dopamine receptor D1(DRD1),a member of the dopamine receptor family,have been indicated to play important roles in cancer progression,but dopamine secretion in hepatocellular carcinoma(HCC)and the effects of DRD1 on HCC remain *** study was designed to explore the contribution of the dopaminergic system to HCC and determine the relationship between DRD1 and prognosis in HCC ***:The dopamine metabolic system was monitored using enzyme-linked immunosorbent assays(ELISAs).The expression of DRD1 was detected by microarray analysis,immunohistochemistry(IHC),and quantitative real-time PCR(qRT-PCR).Stable DRD1 knockout and overexpression cell lines were established for ***,colony formation,and Cell Counting Kit 8(CCK8)assays were performed to assess the malignant behaviors of cancer *** cAMP/PI3K/AKT/cAMP response element-binding(CREB)signaling pathway was evaluated by Western *** pathway,which is agitated by DRD1 in striatal neurons,had been proven to participate in tumor *** HCC tumors were generated for in vivo ***:Dopamine secretion increased locally in HCC due to an imbalance in dopamine metabolism,including the upregulation of dopa decarboxylase(DDC)and the downregulation of monoamine oxidase A(MAOA).Dopamine promoted the proliferation and metastasis of ***1 was highly expressed in HCC tissues and positive DRD1 expression was related to a poor prognosis in HCC *** upregulation of DRD1 agitated malignant activities,including proliferation and metastasis in HCC by regulating the cAMP/PI3K/AKT/CREB pathway,and the downregulation of DRD1 had opposing *** effects of dopamine on HCC was reversed by depleting ***23390,a selective DRD1 antagonist,inhibited the proliferation and metastasis of HCC cells both in vitro and in ***:Dopamine secretion was locally increased in HCC and promoted HCC cell proliferation and ***1

关键词： dopamine dopamine receptor D1 hepatocellular carcinoma prognosis target therapy

Development of LAG-3/FGL1 blocking peptide and combination with radiotherapy for cancer immunotherapy

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Acta Pharmaceutica Sinica B 2024年第3期14卷 1150-1165页

作者： Yuzhen Qian Yixuan Sun Peishang Shi Xiuman zhou Qiongqiong Zhang Qingyu Dong Shengzhe Jin Lu Qiu xiaoshuang Niu Xiaowen zhou Wenshan Zhao Yahong Wu Wenjie Zhai Yanfeng Gao School of Life Sciences Zhengzhou UniversityZhengzhou 450001China School of Pharmaceutical Sciences(Shenzhen) Shenzhen Campus of Sun Yat-sen UniversityShenzhen 518107China International Joint Laboratory for Protein and Peptide Drugs of Henan Province Zhengzhou UniversityZhengzhou 450001China

Aside from antibodies,peptides show great potential as immune checkpoint inhibitors(ICIs)due to several advantages,such as better tumor penetration and lower ***-activation gene 3(LAG-3)is an immune checkpoint which can induce T cell dysfunction through interaction with its soluble ligand fibrinogen like protein-1(FGL1).Here,we found that LAG-3 expression was higher than programmed cell death protein 1(PD-1)in multiple human cancers by TCGA databases,and successfully identified a LAG-3 binding peptide LFP-6 by phage display bio-panning,which specifically blocks the interaction of LAG-3/FGL1 but not LAG-3/***,D-amino acids were introduced to substitute the N-and C-terminus of LFP-6 to obtain the proteolysis-resistant peptide LFP-D1,which restores T cell function in vitro and inhibits tumor growth in ***,a bispecific peptide LFOP targeting both PD-1/PD-L1 and LAG-3/FGL1 was designed by conjugating LFP-D1 with PD-1/PD-L1blocking peptide OPBP-1(8-12),which activates T cell with enhanced proliferation and IFN-γ *** importantly,LFOP combined with radiotherapy significantly improve the T cell infiltration in tumor and elevate systemic antitumor immune *** conclusion,we developed a novel peptide blocking LAG-3/FGL1 which can restore T cell function,and the bispecific peptide synergizes with radiotherapy to further enhance the antitumor immune response.

关键词： LAG-3 FGL1 PD-1 PD-L1 Peptide Immune checkpoint Radiotherapy Cancer immunotherapy