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SFTSV介导的固有免疫应答及其通过抑制宿主蛋白泛素化修饰实现免疫逃逸的调控机制

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病毒学报 2024年第1期40卷 183-196页

作者：王佳敏时小双杨诗寒金宁一李昌杜寿文黑龙江八一农垦大学动物科技学院大庆163319 广东省农业科学院动物卫生研究所广东省畜禽疫病防治研究重点实验室广州510640 中国医学科学院人兽共患病毒病防控关键技术研究创新单元中国农业科学院长春兽医研究所长春130122 人畜共患传染病重症诊治全国重点实验室武汉430030

发热伴血小板减少综合征病毒(Severe fever with thrombocytopenia syndrome virus, SFTSV)感染可造成发热伴血小板减少综合征(Severe fever with thrombocytopenia syndrome, SFTS)。SFTS重症患者大多与SFTSV引起非正常的固有固有免疫反应所导致的细胞因子风暴有关。这些早期固有固有免疫反应包括多种炎症和抗病毒基因的表达,并激活随后的固有固有免疫反应和适应性免疫。感染细胞的早期固有固有免疫反应是由模式识别受体(Pattern recognition receptors, PRRs)、接头蛋白、激酶和转录因子组成的细胞内信号通路所介导的。这些途径受复杂的翻译后修饰网络的严格调节,其中大量的泛素化酶和去泛素化酶使这些修饰可逆且高度动态。这些酶的调控作用可以增强固有免疫反应以抵抗病原体的入侵,也可以抑制其过度活化以避免病理性免疫反应。本文综述了SFTSV引起的早期固有免疫信号通路及其复杂调控机制,为后续针对SFTSV的药物开发和免疫病理学提供了基础。

关键词：新型布尼亚病毒固有免疫 IFN-Ⅰ型信号通路泛素化去泛素化

基于GWO-CNN-LSTM的铁路轨道高低不平顺值反演模型研究

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铁道标准设计 2024年第6期68卷 65-71页

作者：石小双金容鑫杨钢锋尹海涛毛汉领李欣欣广西大学机械工程学院南宁530004 中国铁路南宁局集团有限公司质量技术检测所南宁530004 广西先进制造系统与先进制造技术省级重点实验室南宁530004

为利用列车的车体振动加速度来准确反演铁路的轨道高低不平顺值,提出一种基于灰狼优化算法(GWO)、卷积神经网络(CNN)和长短期记忆网络(LSTM)构建车体振动加速度与轨道高低不平顺值的关系模型(GWO-CNN-LSTM)。首先,将轨检车的实测数据根... 详细信息

为利用列车的车体振动加速度来准确反演铁路的轨道高低不平顺值,提出一种基于灰狼优化算法(GWO)、卷积神经网络(CNN)和长短期记忆网络(LSTM)构建车体振动加速度与轨道高低不平顺值的关系模型(GWO-CNN-LSTM)。首先,将轨检车的实测数据根据轨道检测数据特点,采用莱因达准则进行异常值剔除的预处理;然后,利用处理后的数据以车体振动加速度作为模型的输入,以轨道高低不平顺值作为模型的输出,利用CNN学习车体振动加速度的波形信息,将CNN学习到的特征输入LSTM;最后,再用GWO优化LSTM模型的关键参数,进而反演出轨道高低不平顺值,为突出该模型的适应性,又随机选取了其他区段进行了反演。文中采用3个性能指标来评价模型,并与其他经典的方法进行对比分析。结果表明,GWO-CNN-LSTM模型的均方根误差、平均绝对误差和拟合优度分别为0.141、0.107和0.977,与单一的LSTM相比,GWO-CNN结合LSTM可以使拟合优度提高20.1%;与循环神经网络、BP神经网络和支持向量回归相比,所提出的GWO-CNN-LSTM模型其均方根误差降低68.1%~79.1%,平均绝对误差降低60.4%~68.3%,拟合优度提高27.7%~44.9%,验证了GWO-CNN-LSTM模型用于轨道高低不平顺值反演的有效性。GWO-CNN-LSTM模型为铁路轨道高低不平顺反演研究提供了新的思路。

关键词：铁路轨道轨道高低不平顺灰狼优化算法卷积神经网络长短期记忆网络反演

Effect of Chinese Herbal Medicines on Helicobacter pylori-associated gastroduodenal ulcers: a systematic review and Meta-analysis

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Journal of Traditional Chinese Medicine 2019年第4期39卷 459-465页

作者： shi xiaoshuang Wu Haomeng Ma Xiangxue Yin Xiaolan Li Xia Ma Jinxin Wang Fengyun Tang Xudong Graduate School Beijing University of Chinese MedicineNo.11Beisanhuandong RoadChaoyang DistrictBeijing 100029China Department of Gastroenterology Xiyuan HospitalChina Academy of Chinese Medical SciencesXiyuan RoadHaidian DistrictBeijing 100091China The Second Affiliated Hospital of Guangzhou University of Chinese Medicine 55 Neihuan XiluXiaoguwei StreetPanyu DistrictGuangzhou Higher Education Mega CenterGuangzhou 510006China

OBJECTIVE: To evaluate the effect and safety of Chinese herbal medicines on Helicobacter pylori(HP)-associated gastroduodenal ***: All randomized controlled trials(RCTs)listed in the PubMed, EMBASE, Cochrane Library,China National Knowledge Infrastructure Database,WanFang, China Science and Technology Journal Database and SinoMed databases that were published in English or Chinese were searched, and the retrieval time range was from database inception to December 31, 2018. A comprehensive Meta-analysis of all publications was performed with RevMan 5.3 software, and the quality of the evidence report-ed in the results of Meta-analysis was analyzed with GRADE profiler software(version 3.6.1). Dichotomous data were analyzed by calculating odds ratios and 95% confidence intervals(CIs). Outcome measures included the HP clearance rate and percentage of adverse ***: Eight trials with 919 participants were included in this Meta-analysis. Compared with the effects of single drug therapy on HP-associated gastroduodenal ulcers, according to the statistical analysis, odds ratios for the HP clearance rate and percentage of adverse effects of Chinese herbal medicines administered as complementary medicines combined with drugs were 3.10 [95% CI(2.21, 4.36),P < 0.01] and 0.28 [95% CI(0.15, 0.52), P < 0.01], respectively, and the differences were statistically significant. According to the GRADE analysis, the quality of evidence for the HP clearance rate and percentage of adverse effects were both very ***: Compared with using the drug therapy only, the combination of Chinese herbal medicines with the drug therapy more effectively eliminates HP and alleviates adverse reactions. However,the available studies were of low quality, and therefore more well-designed studies are required to further confirm the findings.

关键词： Peptic ulcer Helicobacter pylori Medicine,Chinese traditional Review Meta-analysis

抗PCV4 Cap蛋白抗体间接ELISA检测方法的建立

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中国动物传染病学报 2024年第1期32卷 115-121页

作者：徐鹏吉卫龙伊立超张爽郝嘉翼高子函任世斌时小双任林柱李昌吉林大学动物科学学院长春130062 中国医学科学院人兽共患病毒病防控关键技术研究创新单元中国农业科学院长春兽医研究所长春130122

为建立可应用于猪圆环病毒4型(PCV4)候选疫苗特异性抗体检测与评价方法,本研究应用PCV4 Cap蛋白作为抗原,以PCV4多克隆兔源抗体作为一抗,优化各反应的最佳条件并建立了针对PCV4 Cap蛋白抗体的间接ELISA方法。最佳条件为2μg/m L PCV4 Ca... 详细信息

为建立可应用于猪圆环病毒4型(PCV4)候选疫苗特异性抗体检测与评价方法,本研究应用PCV4 Cap蛋白作为抗原,以PCV4多克隆兔源抗体作为一抗,优化各反应的最佳条件并建立了针对PCV4 Cap蛋白抗体的间接ELISA方法。最佳条件为2μg/m L PCV4 Cap纯化蛋白,4℃包被过夜,5%脱脂乳封闭60 min,待检血清稀释比例为1∶800,反应条件为37℃、45 min,酶标抗体稀释比例为1∶5000,反应条件为37℃、60 min,底物显色时间为10 min,Cut of f值为0.157,灵敏度可达102400倍。成功建立的抗PCV4Cap蛋白抗体间接ELISA检测方法具有良好的敏感性、重复性和特异性。可为检测PCV4候选疫苗的特异性抗体水平提供一种精准、高效的方法。

关键词：猪圆环病毒4型免疫效果检测间接ELISA方法

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Highly efficient tunable photodetector with a bipolar response in van der Waals heterojunctions

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Science China(Technological Sciences) 2024年第2期67卷 639-646页

作者： shi ChaoFan ZHANG shi XIAO KeNing ZHANG LiBo HAN Li ZHU YuLin TANG WeiWei LIU ChangLong LI GuanHai CHEN xiaoshuang College of Physics and Optoelectronic Engineering Hangzhou Institute for Advanced StudyUniversity of Chinese Academy of Sciences Hangzhou310024China State Key Laboratory of Infrared Physics Shanghai Institute of Technical PhysicsChinese Academy of SciencesShanghai 200083China

The heterojunction integration of two-dimensional(2D)materials via van der Waals(vdW)forces,unencumbered by lattice and processing constraints,constitutes an efficacious approach to enhance the overall optoelectronic performance of photodetectors,due to an assortment of distinctive light-matter ***,vdW heterojunction photodetectors based on transition metal dichalcogenides(TMDs)face an inevitable trade-off between low dark currents and high responsivity,curtailing the application potential of myriad novel optoelectronic components in sensing,spectral,and communication *** this study,we present the successful actualization of a highly sensitive,self-powered,and gate-tunable bipolar response *** mechanisms underlying photocurrent generation were scrutinized via bias-,power-,and position-dependent mapping photoresponse measurements,identifying the photovoltaic effect,which is attributable to the Schottky junction’s built-in electric field,as the predominant *** prototype Au-WS2-graphene photodetector exhibits a remarkable light on/off ratio of 1.2×10^(6),a specific detectivity of 6.12×10^(11)cm H^(z1/2)W^(-1)with 20μs response time at 638 *** wide gate-tunable responsivity provides an adjustability scope,ranging from 0.9 to 3.1 A W^(-1).Notably,the device demonstrates an exceptional linear photocurrent response,with a linear dynamic range(LDR)value approximating 130 dB,which significantly surpasses that of other photodetectors based on TMDs.

关键词： WS2/graphene van der Waals heterojunction gate-tunable bipolar response

Discuss the Properties of Structural Steel and Applications of Waste Concrete from Post-Earthquake Investigations

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Open Journal of Earthquake Research 2013年第2期2卷 32-37页

作者： xiaoshuang shi Qingyuan Wang Yunrong Luo College of Architecture and Environment Sichuan University Chengdu China College of Architecture and Environment Sichuan University Chengdu China College of Mechanical Engineering Sichuan University of Science & Engineering Zigong China

This work proposes two aspects about construction materials abased on Wenchuan post-earthquake investigations. According to different feature failure modes in various damaged structures and the cause of the damage to the effects of the loading during the ground motion, the structural failures were found related to low cycle fatigue (LCF) properties of building steel. The hitherto research development is presented briefly. The characters of cycle response of the steels are tested and discussed. During the post-earthquake reconstruction process, the disposal of huge quantities of earthquake demolition waste brought great challenges. Utilizing the waste concrete taken from earthquake-stricken area as recycled coarse aggregate (RCA) in the new concrete is conducted. Furthermore, the application perspective of RCA is discussed.

关键词： Earthquake Failure Modes Low Cycle Fatigue (LCF) Recycled Coarse Aggregate (RCA)

基于二阶Newton插值的莫尔条纹消除算法

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计算机工程 2020年第5期46卷 259-266页

作者：梁猛史晓霜西安邮电大学电子工程学院西安710121

数码相机拍摄容易受到颜色滤波阵列(CFA)采样频率的规则性图案干扰,导致输出图像带有莫尔条纹。为解决该问题,提出一种基于二阶Newton插值近似的数码图像莫尔条纹消除算法。利用小波变换提取G分量在水平和垂直方向的高频信息,通过对高... 详细信息

数码相机拍摄容易受到颜色滤波阵列(CFA)采样频率的规则性图案干扰,导致输出图像带有莫尔条纹。为解决该问题,提出一种基于二阶Newton插值近似的数码图像莫尔条纹消除算法。利用小波变换提取G分量在水平和垂直方向的高频信息,通过对高频信息做频域变换模拟CFA混叠过程,进而对图像中莫尔区域及其潜在区域进行检测。针对莫尔条纹区域,利用二阶Newton插值获取G分量各方向的估计值,并对所得估计值加权平均取得丢失的G分量,再采用色差空间模型插值恢复R和B分量,最终得到消除莫尔条纹且包含完整RGB信息的图像。实验结果表明,该算法在不影响图像色彩质量的同时能有效去除莫尔条纹,且相比双线性插值、Hibbard等算法恢复图像的峰值信噪比更高、主观视觉效果更好。

关键词：莫尔条纹小波变换频域变换二阶Newton插值色差空间峰值信噪比

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中国长期护理保险基金需求规模预测

中国人口科学 2021年第6期 54-67,127页

作者：陈璐时晓爽南开大学金融学院

文章使用2015和2018年中国健康与养老追踪调查数据,采用Markov模型测算健康状态转移概率,以17个长期护理保险试点地区现行政策为基础,预测2021~2030年中国长期护理保险基金需求规模及变化趋势。研究发现:(1)队列效应显著影响老年人健康... 详细信息

文章使用2015和2018年中国健康与养老追踪调查数据,采用Markov模型测算健康状态转移概率,以17个长期护理保险试点地区现行政策为基础,预测2021~2030年中国长期护理保险基金需求规模及变化趋势。研究发现:(1)队列效应显著影响老年人健康状态转移概率,较晚出生的人口队列具有明显的健康优势和生存优势。(2)2030年中国60岁及以上失能人数将是2021年的1.60倍,其中轻度失能的老年人规模最大,重度失能的老年人增长速度最快,年均增长6.50%。65岁及以上人口中,失能人数增幅最大的为75~79岁组,年均增长6.93%。2030年失能人群中女性将达到4 098.90万人,是男性的1.21倍。(3)按照低方案,2030年中国长期护理保险基金需求规模为1 891.90亿元,约为2021年的2.14倍。高方案下的基金需求规模为低方案的5.66倍。2021~2030年3种方案下的基金需求规模年均增长率为8.68%。测算结果表明,中国长期护理保险基金负担将日益加重。因此文章建议,试点政策的逐步推进要与筹资渠道、筹资水平及整体经济发展状况相适应;将护理服务端口前移,从被动护理转向主动预防,在实现节约基金支出的同时提高老年人生活质量。

关键词： Markov模型队列效应长期护理保险基金规模

Development of LAG-3/FGL1 blocking peptide and combination with radiotherapy for cancer immunotherapy

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Acta Pharmaceutica Sinica B 2024年第3期14卷 1150-1165页

作者： Yuzhen Qian Yixuan Sun Peishang shi Xiuman Zhou Qiongqiong Zhang Qingyu Dong Shengzhe Jin Lu Qiu xiaoshuang Niu Xiaowen Zhou Wenshan Zhao Yahong Wu Wenjie Zhai Yanfeng Gao School of Life Sciences Zhengzhou UniversityZhengzhou 450001China School of Pharmaceutical Sciences(Shenzhen) Shenzhen Campus of Sun Yat-sen UniversityShenzhen 518107China International Joint Laboratory for Protein and Peptide Drugs of Henan Province Zhengzhou UniversityZhengzhou 450001China

Aside from antibodies,peptides show great potential as immune checkpoint inhibitors(ICIs)due to several advantages,such as better tumor penetration and lower ***-activation gene 3(LAG-3)is an immune checkpoint which can induce T cell dysfunction through interaction with its soluble ligand fibrinogen like protein-1(FGL1).Here,we found that LAG-3 expression was higher than programmed cell death protein 1(PD-1)in multiple human cancers by TCGA databases,and successfully identified a LAG-3 binding peptide LFP-6 by phage display bio-panning,which specifically blocks the interaction of LAG-3/FGL1 but not LAG-3/***,D-amino acids were introduced to substitute the N-and C-terminus of LFP-6 to obtain the proteolysis-resistant peptide LFP-D1,which restores T cell function in vitro and inhibits tumor growth in ***,a bispecific peptide LFOP targeting both PD-1/PD-L1 and LAG-3/FGL1 was designed by conjugating LFP-D1 with PD-1/PD-L1blocking peptide OPBP-1(8-12),which activates T cell with enhanced proliferation and IFN-γ *** importantly,LFOP combined with radiotherapy significantly improve the T cell infiltration in tumor and elevate systemic antitumor immune *** conclusion,we developed a novel peptide blocking LAG-3/FGL1 which can restore T cell function,and the bispecific peptide synergizes with radiotherapy to further enhance the antitumor immune response.

关键词： LAG-3 FGL1 PD-1 PD-L1 Peptide Immune checkpoint Radiotherapy Cancer immunotherapy