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Engineering G-quadruplex aptamer to modulate its binding specificity

National Science Review 2021年第4期8卷 107-113页

作者： Long Li Shujuan Xu Xueyu Peng Yuzhuo Ji He Yan Cheng Cui Xiaowei Li xiaoshu pan Lu Yang Liping Qiu Jianhui Jiang Weihong Tan Department of Chemistry and Department of Physiology and Functional Genomics Center for Research at the Bio/Nano Interface Health Cancer Center UF Genetics InstituteMcKnight Brain Institute University of Florida Molecular Science and Biomedicine Laboratory State Key Laboratory of Chemo/Biosensing and ChemometricsCollege of Chemistry and Chemical Engineering College of Biology Aptamer Engineering Center of Hunan ProvinceHunan University Molecular Science and Biomedicine Laboratory(MBL) State Key Laboratory of Chemo/Biosensing and ChemometricsCollege of Chemistry and Chemical Engineering College of Biology Aptamer Engineering Center of Hunan ProvinceHunan University Institute of Molecular Medicine(IMM) Renji HospitalShanghai Jiao Tong University School of Medicine and College of Chemistry and Chemical EngineeringShanghai Jiao Tong University

The use of aptamers in bioanalytical and biomedical applications exploits their ability to recognize cell surface protein receptors. Targeted therapeutics and theranostics come to mind in this regard. However,protein receptors occur on both cancer and normal cells; as such, aptamers are now taxed with identifying high vs. low levels of protein expression. Inspired by the flexible template mechanism and elegant control of natural nucleic acid-based structures, we report an allosteric regulation strategy for constructing a structure-switching aptamer for enhanced target cell recognition by engineering aptamers with DNA intercalated motifs(i-motifs) responsive to the microenvironment, such as p H. Structure-switching sensitivity can be readily tuned by manipulating i-motif sequences. However, structure-switching sensitivity is difficult to estimate, making it equally difficult to effectively screen modified aptamers with the desired sensitivity. To address this problem, we selected a fluorescent probe capable of detecting G-quadruplex in complicated biological media.

关键词： G-quadruplex aptamer i-motif binding specificity cell microenvironment

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Lipid–oligonucleotide conjugates for bioapplications

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National Science Review 2020年第12期7卷 1933-1953页

作者： Xiaowei Li Kejun Feng Long Li Lu Yang xiaoshu pan Hoda Safari Yazd Cheng Cui Juan Li Leonid Moroz Yujia Sun Bang Wang Xiang Li Tong Huang Weihong Tan Center for Research at Bio/Nano Interface Department of Chemistry and Department of Physiology and Functional GenomicsHealth Cancer CenterUF Genetics Institute and Mc Knight Brain InstituteUniversity of Florida School of Chemistry and Materials Engineering Huizhou University The Cancer Hospital of the University of Chinese Academy of Sciences Institute of Cancer and Basic Medicine(IBMC) Chinese Academy of Sciences Molecular Science and Biomedicine Laboratory (MBL) State Key Laboratory for Chemo/BioSensing and ChemometricsCollege of Chemistry and Chemical EngineeringCollege of Biologyand Aptamer Engineering Center of Hunan ProvinceHunan University

Lipid–oligonucleotide conjugates(LONs) are powerful molecular-engineering materials for various applications ranging from biosensors to *** unique amphiphilic structures enable the self-assembly and the conveyance of information with high *** particular,LONs present remarkable potential in measuring cellular mechanical forces and monitoring cell *** are also essential sensing tools for intracellular imaging and have been employed in developing cell-surface-anchored DNA nanostructures for biomimetic-engineering *** incorporating therapeutic oligonucleotides or small-molecule drugs,LONs hold promise for targeted ***,LONs mediate the controllable assembly and fusion of vesicles based on DNA-strand displacements,contributing to nanoreactor construction and macromolecule *** this review,we will summarize the general synthesis strategies of LONs,provide some characterization analysis and emphasize recent advances in bioanalytical and biomedical *** will also consider the relevant challenges and suggest future directions for building better functional LONs in nanotechnology and materials-science applications.

关键词： lipid–oligonucleotide DNA nanostructure biosensor biomedicine bioanalysis nanoreactor

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Defective Nickel-Iron Layered Double Hydroxide for Enhanced Photocatalytic NO Oxidation with Significant Alleviation of NO2 Production

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Engineering 2024年第5期36卷 276-284页

作者： Xiaoyu Li xiaoshu Lv Jian pan Peng Chen Huihui Peng Yan Jiang Haifeng Gong Guangming Jiang Li’an Hou Engineering Research Center for Waste Oil Recovery Technology and Equipment Ministry of EducationChongqing Technology and Business UniversityChongqing 400067China State Key Laboratory of Water Environment Simulation School of EnvironmentBeijing Normal UniversityBeijing 100875China Environmental Technology Innovation Center of Jiande Hangzhou Bertzer Catalyst Co.Ltd.Hangzhou 310058China Chongqing Key Laboratory of Green Synthesis and Application College of ChemistryChongqing Normal UniversityChongqing 401331China

Photocatalysis offers a sustainable means for the oxidative removal of low concentrations of NOx(NO,NO2,N2O,N2O5,etc.)from the *** double hydroxides(LDHs)are promising candidate photocatalysts owing to their unique layered and tunable chemical structures and abundant surface hydroxide(OH)moieties,which are hydroxyl radical(OH)***,the practical applications of LDHs are limited by their poor charge-separation ability and insufficient active ***,we developed a facile N_(2)H_(4)-driven etching approach to introduce dual Ni^(2+)and OHvacancies(Niv and OHv,respectively)into NiFe-LDH nanosheets(hereafter referred to as NiFe-LDH-et)to facilitate improved charge-carrier separation and active Lewis acidic site(Fe^(3+)and Ni^(2+)exposed at OHv)*** contrast to inert pristine LDH,NiFe-LDH-et actively removed NO under visible-light ***,Ni_(76)Fe_(24)-LDH-et etched with 1.50 mmol·L^(-1)N_(2)H_(4)solution removed 32.8%of the NO in continuously flowing air(NO feed concentration:500 parts per billion(ppb))under visible-light illumination,thereby outperforming most reported *** and theoretical data revealed that the dual vacancies promoted the production of reactive oxygen species(O_(2)·^(-)andOH)and the adsorption of NO on the *** situ spectroscopy demonstrated that NO was preferentially adsorbed at Lewis acidic sites,particularly exposed Fe^(3+)sites,converted into NO+,and subsequently oxidized to NO3without the notable formation of the more toxic intermediate NO2,thereby alleviating risks associated with its production and emission.

关键词： Vacancie Layered double hydroxide NO+ Photocatalysis NO removal

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AI-Based Advanced Approaches and Dry Eye Disease Detection Based on Multi-Source Evidence:Cases,Applications,Issues,and Future Directions

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Big Data Mining and Analytics 2024年第2期7卷 445-484页

作者： Mini Han Wang Lumin Xing Yi pan Feng Gu Junbin Fang Xiangrong Yu Chi Pui pang Kelvin Kam-Lung Chong Carol Yim-Lui Cheung Xulin Liao Xiaoxiao Fang Jie Yang Ruoyu Zhou xiaoshu Zhou Fengling Wang Wenjian Liu Zhuhai People’s Hospital(Zhuhai Hospital Affiliated with Jinan University/The First Affiliated Hospital of Faculty of Medicine Macao University of Science and Technology) Zhuhai 519000China Department of Ophthalmology and Visual Sciences The Chinese University of Hong KongHong Kong 999077China Faculty of Data Science City University of MacaoMacao 999078China Zhuhai Institute of Advanced Technology Chinese Academy of Sciences Zhuhai 519000China Perspective Technology Group Zhuhai 519000China First Affiliated Hospital of Shandong First Medical University Shandong Provincial Qianfoshan Hospital Jinan 250000China Shenzhen Institute of Advanced Technology Chinese Academy of SciencesShenzhen 518000China College of Staten Island The City University of New YorkNY 10314USA Department of Optoelectronic Engineering Jinan UniversityGuangzhou 510000China Zhuhai Aier Eye Hospital Zhuhai 519000China College of Artificial Intelligence Chongqing Industry and Trade PolytechnicChongqing 400000China Centre for Science and Technology Exchange and Cooperation between China and Portuguese-Speaking Countries Zhuhai 519000China School of Artificial Intelligence Hezhou UniversityHezhou 542899China

This study explores the potential of Artificial Intelligence(AI)in early screening and prognosis of Dry Eye Disease(DED),aiming to enhance the accuracy of therapeutic approaches for eye-care *** the promising opportunities,challenges such as diverse diagnostic evidence,complex etiology,and interdisciplinary knowledge integration impede the interpretability,reliability,and applicability of AI-based DED detection *** research conducts a comprehensive review of datasets,diagnostic evidence,and standards,as well as advanced algorithms in AI-based DED detection over the past five *** DED diagnostic methods are categorized into three groups based on their relationship with AI techniques:(1)those with ground truth and/or comparable standards,(2)potential AI-based methods with significant advantages,and(3)supplementary methods for AI-based DED *** study proposes suggested DED detection standards,the combination of multiple diagnostic evidence,and future research directions to guide further ***,the research contributes to the advancement of ophthalmic disease detection by providing insights into knowledge foundations,advanced methods,challenges,and potential future perspectives,emphasizing the significant role of AI in both academic and practical aspects of ophthalmology.

关键词： Artificial Intelligence(AI) ophthalmology Dry Eye Disease(DED)detection multi-source evidence

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常规注塑机上实现树脂镜片加工的方法

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武汉理工大学学报 2001年第10期23卷 56-58,62页

作者：潘晓曙温州市工业科学研究院

镜片加工中 ,必须要很好地控制其曲光度、透光度及收缩变形等参数。树脂镜片如果用昂贵的专机生产 ,势必增加成本。通过对常规注塑机进行简单的改造 ,就可实现各类镜片的加工。

关键词：聚丙烯酸树脂 PMMA 成型工艺参数树脂镜片注塑机眼镜镜片加工

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Research and simulation on synchronization algorithm of PSS in a 5G system based on block cross-correlation

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Journal of Southeast University(English Edition) 2021年第3期37卷 245-250页

作者： Zheng Ruimen Wang Xiaojun pan Wei Chen xiaoshu National Mobile Communications Research Laboratory Southeast University Nanjing 210096 China

In response to the downlink synchronization requirements of the user equipment(UE)or third-party radio equipment in fifth-generation(5G)mobile communication systems,a synchronization algorithm of primary synchroni-zation signal(PSS)was designed and developed in the 5G system based on block *** to the new characteristics of the 5G synchronization channel and broadcast channel,starting from the traditional downlink synchronization algorithm of long-term evolution(LTE),the detection performance of the algorithm under a low signal-to-noise ratio(SNR)is improved by introducing an incoherent accumulation,and the new scheme of joint coarse frequency offset estimation is used to improve the frequency offset estimation ***,the performance of the proposed synchronization algorithm is verified by conducting a simulation on a 5G downlink simulation platform based on MATLAB *** results show that the improved downlink synchronization algorithm has stable performance in the tapped delay line-C(TDL-C)and additive white Gaussian noise(AWGN)channels with large frequency deviation and low SNR.

关键词： fifth-generation(5G)cell search downlink synchronization cross-correlation detection frequency offset estimation

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广州批发市场的供应物流空间格局及其形成机制

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地理学报 2012年第2期67卷 179-188页

作者：潘裕娟曹小曙中山大学地理科学与规划学院广州510275

城市批发市场是集商流、物流和信息流于一体的商品集散地,其产生的物流活动属于城市物流的一部分。结合实地调查数据,采用社会网络分析方法对广州市批发市场的供应物流空间格局进行研究。首先构建由19个供应地和21个接收地组成的2-模矩... 详细信息

城市批发市场是集商流、物流和信息流于一体的商品集散地,其产生的物流活动属于城市物流的一部分。结合实地调查数据,采用社会网络分析方法对广州市批发市场的供应物流空间格局进行研究。首先构建由19个供应地和21个接收地组成的2-模矩阵,将其反应的关系信息进行可视化表达,结果显示物流结点呈现出交通指向性、地域集中性以及圈层式布局特点;货物供应以外向型为主,并形成国外、省外城市、珠三角和粤北地区的主要供应流格局;结点集货规模等级差异明显,形成城市内部的反y形集货格局。然后对物流网络进行中心性分析,通过测量度数中心度、接近中心度和中间中心度等指标,得出珠三角、省外城市和白云区是物流网络的核心供应地;流花服装和中大布匹批发市场群是物流网络的核心接收地;南沙区、黄埔茅岗批发市场群等则属于物流网络的边缘结点。最后从城市性质、城市空间发展、产业集群和物流服务等4方面对供应物流空间格局的形成机制进行探讨。通过对批发市场货流网络空间的研究,有利于为今后城市物流空间优化提供一定的决策参考依据。

关键词：广州批发市场供应物流空间格局社会网络分析

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A Novel Method for Measuring the Coarse Water Droplets in Wet Steam Flow in Steam Turbines

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Journal of Thermal Science 2001年第2期10卷 123-126,132页

作者： xiaoshu Cai Lin Wang Yongzhi pan Xin Ouyan Jianqi Shen Faculty of Power Engineering, University of Shanghai for Science & Technology (USST), 516 Jungong Road, Shanghai 200093, China E-mail: caixs@*** Faculty of Power Engineering University of Shanghai for Science & Technology (USST) Shanghai China

Some optical probes based on light extinction have been developed to measure wetness dominated with fine droplets in steam turbine. However, coarse water droplets (hereafter referred to as CWD) that are the main cause of erosion of blade and of wetness loss of steam turbine can’t be detected by the extinction probes because of its large size. In this paper, a new method - the light fluctuation method is presented that is capable of measuring the she of CWD. A new probe based on this method was developed for measuring the she of CWD as well as its velocity and concentration.

关键词： steam turbine coarse water measurement light fluctuation method.

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跨微米尺度混合颗粒粒径的同步测量方法

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光学学报 2020年第7期40卷 102-108页

作者：赵蓉潘科玮杨斌平力蔡小舒上海理工大学上海市动力工程多相流动与传热重点实验室上海200093 上海航天动力技术研究所发动机技术发展研究室上海201109

基于多波长消光法和图像法颗粒测量原理,提出了跨微米尺度混合颗粒粒径同步测量方法,建立了亚微米-十微米尺度颗粒粒径消光光谱反演算法及十微米以上尺度颗粒粒径图像处理算法;采用分光棱镜,搭建了消光光谱与背光图像同步测量装置,利用5... 详细信息

基于多波长消光法和图像法颗粒测量原理,提出了跨微米尺度混合颗粒粒径同步测量方法,建立了亚微米-十微米尺度颗粒粒径消光光谱反演算法及十微米以上尺度颗粒粒径图像处理算法;采用分光棱镜,搭建了消光光谱与背光图像同步测量装置,利用500 nm^76.9μm粒径范围内的10种标准颗粒配成跨微米尺度混合颗粒样品并开展实验研究。结果表明:利用所提方法开展跨微米尺度混合颗粒粒径同步测量时,亚微米-十微米尺度颗粒消光光谱与十微米以上尺度颗粒背光图像的相互影响可忽略,可同步测量得到跨微米尺度混合颗粒粒径;利用消光法和图像法分别开展亚微米-十微米、十微米以上尺度颗粒粒径测量,与标准颗粒粒径相比,相对误差均小于8%,且测量重复性较好,这为跨微米尺度混合颗粒提供了一种有效的粒径测量手段。

关键词：测量多波长消光法图像法跨微米尺度混合颗粒粒径测量

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“钻石理论”与区域中心城市的物流业分析

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城市观察 2009年第2期 146-154页

作者：潘裕娟曹小曙中山大学中山大学地理科学与规划学院

本文运用迈克尔·波特的"钻石理论"对广州物流业的竞争优势进行分析。在此基础上,构建物流业竞争优势的指标体系,并选取全国十大城市进行因子分析,得出广州物流业的竞争优势位居全国前列,仅落后于上海和北京。文章试图通过对竞争优势的... 详细信息

本文运用迈克尔·波特的"钻石理论"对广州物流业的竞争优势进行分析。在此基础上,构建物流业竞争优势的指标体系,并选取全国十大城市进行因子分析,得出广州物流业的竞争优势位居全国前列,仅落后于上海和北京。文章试图通过对竞争优势的全面分析,理清广州物流业的现状态势,并为有关部门决策提供一定参考。

关键词：广州物流业竞争优势钻石理论因子分析

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