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Intrathecal MK-801 inhibits formalin-induced activation of spinal p38-MAPK in rats

Neural Regeneration Research 2008年第11期3卷 1168-1171页

作者： Zhifeng Peng Xin Zhao Xing Jin Xiaochun Yan xiaorong yang Ce Zhang Department of Physiology and Neurology Shanxi Medical University Taiyuan 030001 Shanxi Province China

BACKGROUND： p38 mitogen-activated protein kinase （MAPK） plays an instrumental role in signal transduction from the cell surface to the nucleus, while subcutaneous injection of formalin can induce increased activation of spinal p38 MAPK. However, the mechanisms underlying the formalin-induced activation of spinal p38 MAPK in rats are unclear. OBJECTIVE： To observe the effects of N-methyl-D-aspartic acid （NMDA） receptor antagonist MK-801 on the formalin-induced activation of spinal p38 MAPK in rats. DESIGN, TIME AND SETTING： This randomized grouping, controlled animal experiment was performed at the Department of Physiology and Neurobiology, Shanxi Medical University between May and November 2007. MATERIALS： Forty eight healthy, adult Wistar rats were randomly divided into two groups： formalin ＋ normal saline （n = 12） and formalin ＋ MK-801 （n = 36）. The formalin ＋ MK-801 group was further divided into three subgroups according to the dosage of MK-801 （10, 50, and 100 nmol/L, 12 rats for each subgroup） METHODS： Following anesthesia, polyethylene tubing filled with sterile normal saline was implanted into the subarachnoid cavity. On postoperative days 5-8, rats received a 15 minute perfusion of normal saline or MK-801 （10, 50, and 100 nmol/L） in the formalin ＋ normal saline and formalin ＋ MK-801 groups, respectively, followed by formalin injection for the induction of nociceptive behavior. MAIN OUTCOME MEASURES： Detection of total p38 MAPK and of phosphorylated p38 MAPK by western Blot analysis; observation of nociceptive behaviors in the 1 hour after formalin injection. RESULTS： Western Blot analysis revealed that injection of formalin had no effect on total p38 MAPK expression but resulted in increased phosphorylation of p38 MAPK in the spinal cord. This increase was apparent after 5 minutes, peaked at 20 minutes, and thereafter descended and reached control levels after 45 minutes. Pretreatment with MK-801 （10, 50, 100 nmol/L） resulted in a dose-dependent reduction o

关键词： NMDA receptor antagonist MK-801 formalin nociceptive behavior phosphorylation p38 MAPK

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TXNIP、NLRP3在冠状动脉粥样硬化斑块中的表达情况及其与冠心病猝死的关系

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重庆医学 2024年第15期53卷 2284-2290页

作者：汪家文杨林闵海王玉杨丽陈再翠戴佳琳杨小蓉王杰贵州医科大学法医学院/法医司法鉴定中心贵阳550004 贵州医科大学生物与工程学院/健康医药现代产业学院贵阳550025

目的探讨TXNIP、NLRP3在冠状动脉粥样硬化斑块中的表达及其与斑块继发病变、冠心病猝死的关系。方法回顾性分析2019年1月至2022年3月贵州医科大学法医司法鉴定中心经尸体解剖提取的105例心脏冠状动脉标本及来源者相关资料。根据冠状动... 详细信息

目的探讨TXNIP、NLRP3在冠状动脉粥样硬化斑块中的表达及其与斑块继发病变、冠心病猝死的关系。方法回顾性分析2019年1月至2022年3月贵州医科大学法医司法鉴定中心经尸体解剖提取的105例心脏冠状动脉标本及来源者相关资料。根据冠状动脉有无硬化斑块将其分为无病变组(n=20)和斑块组(n=85),再根据冠状动脉是否存在继发病变及其是否为冠心病猝死案例将斑块组分为非冠心病猝死组(n=25)、冠心病猝死无继发病变组(n=30)和冠心病猝死合并继发病变组(n=30)。苏木素-伊红(HE)染色制片及利用IPP6.0图像分析软件测量冠状动脉内膜及病灶厚度、纤维帽厚度、坏死灶厚度及管腔狭窄程度。免疫组织化学法、Western blot和实时荧光定量逆转录-PCR(qRT-PCR)检测冠状动脉TXNIP、NLRP3分布特点及表达情况。结果与无病变组比较,其余3组内膜及病灶厚度、纤维帽厚度、坏死灶厚度更厚,管腔狭窄程度更高,差异有统计学意义(P<0.05)。与冠心病猝死无继发病变组比较,冠心病猝死合并继发病变组内膜及病灶厚度、坏死灶厚度更厚,管腔狭窄程度更高,差异有统计学意义(P<0.05)。无病变组冠状动脉管壁未见TXNIP、NLRP3蛋白表达。非冠心病猝死组TXNIP、NLRP3蛋白强阳性表达率为40.0%、36.0%,弱阳性表达率为32.0%、36.0%,较弱阳性表达率为28.0%、28.0%;冠心病猝死无继发病变组强阳性表达率为50.0%、43.3%,较强阳性表达率为33.3%、36.7%,弱阳性表达率为16.7%、20.0%;冠心病猝死合并继发病变组强阳性表达率为73.3%、76.7%,较强阳性表达率为26.7%、23.3%。冠心病猝死合并继发病变组冠状动脉TXNIP、NLRP3蛋白、mRNA表达水平高于其余3组,差异有统计学意义(P<0.05)。冠状动脉斑块内TXNIP与NLRP3表达的吸光度值、蛋白及mRNA水平呈正相关(P<0.05)。TXNIP、NLRP3表达水平与内膜及病灶厚度呈正相关,与纤维帽厚度呈负相关(P<0.05);冠状动脉坏死灶厚度与TXNIP、NLRP3呈正相关(P<0.05)。结论TXNIP、NLRP3可作为冠心病猝死的诊断指标。

关键词：冠状动脉粥样硬化斑块猝死 TXNIP NLRP3 诊断

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Effects of pre-moxibustion at Zusanli (ST36) on heat shock protein 70 expression in rats with gastric mucosal lesions after neurotomy

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Neural Regeneration Research 2012年第30期7卷 2370-2376页

作者： Liang Peng Mi Liu xiaorong Chang Zhou yang Shouxiang Yi Jie Yan Yan Peng College of Acupuncture and Tuina Hunan University of Chinese Medicine Changsha 410007 Hunan Province China

Studies have shown that pre-moxibustion protects the gastric mucosa by up-regulating the expression of heat shock protein 70. However, the signaling pathway underlying this effect remains unclear. Rats were intragastrically administered absolute alcohol, causing obvious lesion of the gastric mucosa. Following pre-moxibustion at Zusanfi （ST36） for 8 days, the ulcer index decreased to different degrees. The results of an enzyme linked immunosorbent assay and western blotting showed significant upregulation of heat shock protein 70 expression in the gastric mucosa and serum. None out of transection of the spinal cord, damage to the nucleus of the solitary tract, neurotomy of the vagal nerve and neurotomy of the common peroneal nerve affected the decrease in ulcer index or the increase in heat shock protein 70 expression in serum after pre-moxibustion at Zusanfi, and heat shock protein 70 expression was obviously decreased in the gastric mucosa. These findings suggest that pre-moxibustion at Zusanfi can protect the gastric mucosa against lesioning, and that the mechanism underlying this effect involves its induction of heat shock protein 70 expression. Neural pathways participate in the regulatory effects of moxibustion on heat shock protein 70 expression in the gastric mucosa.

关键词： pre-moxibustion transection of neural pathway Zusanli (ST36) gastric mucosal lesion heat shockproteins-70 ulcer index traditional Chinese medicine neural regeneration

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Surveillance and examination of microbial contamination in ice cream in China

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Food Quality and Safety 2022年第3期6卷 446-453页

作者： Lin Yan Xiaoyan Pei Junjie Miao Ying Li Shuran yang Zixin Peng xiaorong yang Lingling Mei Qingwen yang Huan Ren Dajin yang Hongmei Shi Hebei Key Laboratory of Environment and Human Health School of Public HealthHebei Medical UniversityShijiazhuangChina China National Center for Food Safety Risk Assessment(CFSA) BeijingChina Sichuan Provincial Center for Disease Control and Prevention ChengduChina Zhejiang Provincial Center for Disease Control and Prevention HangzhouChina Yunnan Provincial Center for Disease Controland Prevention KunmingChina

This study investigated the microbial contamination of ice cream in China.A total of 2887 ice cream samples were collected from different regions of *** by the aerobic plate count(APC),coliforms,and three foodborne pathogens,Listeria monocytogenes(***),Staphylococcus aureus(***),and Salmonella spp.,was detected in the *** isolates were further analyzed for antibiotic susceptibility and multi-locus sequence typing(MLST).The results showed that APC was more than 10^(5) colony forming units(CFU)/g in 6.10%(176/2887)and coliform was more than 10^(2) CFU/g in 15.69%(453/2887)of all *** positive rates of ***,***,and Salmonella *** 0.66%,0.62%,and 0.10%,*** these,*** contamination was more than 102 CFU/g in two samples,and *** in the positive sample was in the range of 0.3-240 most probable number(MPN)/*** a median of 4.3 MPN/*** hygienic status of the packaged samples was much better than that of the bulk samples(P0.05).For 18 *** strains isolated from ice cream,the resistance rate of nine antibiotics was 5.56%(1/18).By MLST,the *** strains were classified into nine sequence types(STs),of which ST8 was the most common(six isolates).These results indicate that a potential health risk to the public may be caused by ice cream,particularly in susceptiblepopulations.

关键词： Frozen dessert microbial contamination foodborne pathogens antibiotic susceptibility multi-locus sequence typing

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Toehold链置换辅助智能手机比色法特异性检测废水中新冠病毒靶标RNA

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分析试验室 2024年第1期43卷 36-41页

作者：张琳张颖宋九华韩耀霞杨孝容雷欣梦黄莉翔徐君茹何思仪邱小凤史铠乐山师范学院新能源材料与化学学院乐山614000

基于Toehold介导的链置换反应(TSDR)与DNA酶,构建了一个强特异性及低成本检测废水中SARS-CoV-2靶标RNA单碱基突变的比色生物传感器。SARS-CoV-2靶标RNA与双链DNA(dsDNA)引发TSDR,释放出完整的G-四链体序列并与氯化血红素(Hemin)结合,形... 详细信息

基于Toehold介导的链置换反应(TSDR)与DNA酶,构建了一个强特异性及低成本检测废水中SARS-CoV-2靶标RNA单碱基突变的比色生物传感器。SARS-CoV-2靶标RNA与双链DNA(dsDNA)引发TSDR,释放出完整的G-四链体序列并与氯化血红素(Hemin)结合,形成G-四链体/Hemin DNA酶,进而催化双氧水氧化3,3’,5,5’-四甲基联苯胺,使溶液呈蓝色。当SARS-CoV-2靶标RNA存在单碱基突变时,TSDR被抑制,导致裸眼观察到的溶液颜色与未突变异的靶标相比更浅。同时采用智能手机摄像头捕捉溶液的RGB值变化(ΔRGB),对裸眼观察结果的准确性进行验证。在最优条件下,构建的比色生物传感器能够特异性识别单个碱基突变的SARS-CoV-2靶标RNA,具有用于废水中SARS-CoV-2变异毒株检测的潜力。

关键词：新冠病毒靶标RNA Toehold链置换智能手机废水

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Organ-specific efficacy in advanced non-small cell lung cancer patients treated with first-line single-agent immune checkpoint inhibitors

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Chinese Medical Journal 2022年第12期135卷 1404-1413页

作者： Jiayi Deng Ming Gao Qing Gou Chongrui Xu Honghong Yan Mingyi yang Jiakang Li xiaorong yang Xuewu Wei Qing Zhou School of Medicine South China University of TechnologyGuangzhouGuangdong 511400China Guangdong Lung Cancer Institute Guangdong Provincial People's HospitalGuangdong Academy of Medical SciencesGuangzhouGuangdong 510080China The Second School of Clinical Medicine Southern Medical UniversityGuangzhouGuangdong 510515China

Background:Response to immune checkpoint inhibitors(ICIs)is affected by multiple *** study aimed to explore whether sites of metastasis are associated with clinical outcomes of ICIs in advanced non-small-cell lung cancer(NSCLC)***::The data of NSCLC patients with high programmed death-ligand 1 expression and good performance status receiving first-line ICIs monotherapy from Guangdong Provincial People’s Hospital between May 2019 and July 2020 were retrospectively *** sites included liver,bone,brain,adrenal gland,pleura,and contralateral ***-free survival(PFS)and overall survival(OS)were compared between different metastatic sites and metastatic burden by the Kaplan-Meier ***-specific disease control rate(OSDCR)of different individual metastatic sites was ***:Forty NSCLC patients meeting the criteria were *** presence of liver metastasis was significantly associated with shorter PFS(3.1 vs.15.5 months,P=0.0005)and OS(11.1 months *** reached,P=0.0016).Besides,patients with bone metastasis tend to get shorter PFS(4.2 vs.15.5 months,P=0.0532)rather than OS(P=0.6086).Moreover,the application of local treatment could numerically prolong PFS in patients with brain metastasis(15.5 vs.4.3 months,P=0.1894).More metastatic organs involved were associated with inferior PFS(P=0.0052)but not OS(P=0.0791).The presence of liver metastasis or bone metastasis was associated with more metastatic organs(Phi[φ]:0.516,P=0.001).The highest OSDCR was observed in lung(15/17),and the lowest in the liver(1/4).Conclusions:Metastases in different anatomical locations may be associated with different clinical outcomes and local tumor response to ICIs in *** monotherapy shows limited efficacy in patients with liver and bone metastasis,thus patients with this type of metastasis might require more aggressive combination strategies.

关键词： Metastatic sites Immunotherapy Liver metastases Bone metastasis Non-small-cell lung cancer Tumor response

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课程思政在病理学教学中的应用与探索

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中国继续医学教育 2024年第10期16卷 181-185页

作者：袁丹姚晋杨晓荣王进京梁娜遵义医科大学附属医院病理科贵州遵义563000 遵义医科大学第一临床学院病理学教研室贵州遵义563000 遵义医科大学基础医学院组织学与胚胎学教研室贵州遵义563000

新时代背景下,为了落实立德树人的根本任务,对医学院校的思想政治教育工作提出了更高的要求。立足学科课程特点基础上,加强医学生思政教育,全面推进课程思政建设是首要途径。文章在充分把握病理学学科特点、教学大纲及培养目标的基础上... 详细信息

新时代背景下,为了落实立德树人的根本任务,对医学院校的思想政治教育工作提出了更高的要求。立足学科课程特点基础上,加强医学生思政教育,全面推进课程思政建设是首要途径。文章在充分把握病理学学科特点、教学大纲及培养目标的基础上,通过深挖病理教学过程中的思政元素、建设系统的病理学课程思政的教学体系、培养专业的课程思政教师队伍,同时改变病理学传统“填鸭式”等教学方法,提出和应用以问题为基础的教学模式(problem-based learning,PBL),以案例为基础的教学模式(case-based learning,CBL)、翻转课堂、微信网络平台及直播平台等新的教学方法,将思政元素结合一定的教学方法和教辅工具(如实验室标本)润物细无声地融入病理学课堂,既能提高学生学习的主动性和积极性,同时也能达到对医学生思想政治的教育的目的,从而培养三观正、专业知识扎实、医德好的新时代医学精英,努力推动教学的改革与创新。

关键词：课程思政病理学学科特点立德树人以案例为基础的教学模式翻转课堂

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Changing trends of disease burden of gastric cancer in China from 1990 to 2019 and its predictions:Findings from Global Burden of Disease Study

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Chinese Journal of Cancer Research 2021年第1期33卷 11-26页

作者： Tongchao Zhang Hui Chen Xiaolin Yin Qiufeng He Jinyu Man xiaorong yang Ming Lu Department of Epidemiology and Health Statistics School of Public HealthCheeloo College of MedicineShandong UniversityJinan 250012China Clinical Epidemiology Unit Qilu Hospital of Shandong UniversityJinan 250012China Clinical Research Center of Shandong University Jinan 250012China

Objective:China is one of the countries with the heaviest burden of gastric cancer(GC)in the *** the epidemiological trends and patterns of GC in China can contribute to formulating effective prevention ***:The data on incidence,mortality,and disability-adjusted life-years(DALYs)of GC in China from1990 to 2019 were obtained from the Global Burden of Disease Study(2019).The estimated annual percentage change(EAPC)was calculated to evaluate the temporal trends of disease burden of GC,and the package Nordpred in the R program was used to perform an age-period-cohort analysis to predict the numbers and rates of incidence and mortality in the next 25 ***:The number of incident cases of GC increased from 317.34 thousand in 1990 to 612.82 thousand in2019,while the age-standardized incidence rate(ASIR)of GC decreased from 37.56 per 100,000 in 1990 to 30.64 per 100,000 in 2019,with an EAPC of-0.41[95%confidence interval(95%CI):-0.77,-0.06].Pronounced temporal trends in mortality and DALYs of GC were *** the next 25 years,the numbers of new GC cases and deaths are expected to increase to 738.79 thousand and 454.80 thousand,respectively,while the rates of incidence and deaths should steadily *** deaths and DALYs attributable to smoking were different for males and ***:In China,despite the fact that the rates of GC have decreased during the past three decades,the numbers of new GC cases and deaths increased,and will continue to increase in the next 25 *** strategies are needed to reduce the burden of GC,such as screening and early detection,novel treatments,and the prevention of risk factors.

关键词： Gastric cancer disease burden temporal trend risk factor prediction

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Involvement of mitogen-activated protein kinase pathways in N-methyl-D-aspartate-induced excitotoxicity

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Neural Regeneration Research 2011年第4期6卷 288-294页

作者： xiaorong yang Ping Sun Huaping Qin Rui Wang Ye Wang Ruihong Shi Xin Zhao Ce Zhang Department of Physiology Shanxi Medical University Taiyuan 030001 Shanxi Province China Department of Nutrition the First Hospital Shanxi Medical University Taiyuan 030001 Shanxi Province China

Previous studies have shown that mitogen-activated protein kinase （MAPK） signaling pathways are involved in N-methyI-D-aspartate （NMDA）-mediated excitotoxicity. However, a systematic observation or analysis of the role of these various MAPK pathways in excitotoxicity processes does not exist. The present study further evaluated the role and contribution of three MAPK pathways extracellular signal-regulated kinase, c-Jun N-terminal kinase, and p38 MAPK in an NMDA-mediated excitotoxicity model using MAPK^specific inhibitor. Results demonstrated that c-Jun N-terminal kinase inhibitor SP600125 and/or p38 MAPK inhibitor SB203580 inhibited NMDA-induced reduction in cell viability, as well as reduced NMDA-induced lactate dehydrogenase leakage and reactive oxygen species production. However, PD98059, an inhibitor of extracellular signal-regulated kinase, did not influence this model. Results demonstrated an involvement of c-Jun N-terminal kinase and p38 MAPK, but not extracellular signal-regulated kinase, in NMDA-mediated excitotoxicity in cortical neurons.

关键词： cortical neurons excitotoxicity mitogen-activated protein kinase N-methyI-D-aspartate

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广州市5892例女性人乳头瘤病毒感染和基因分型特点分析

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中国预防医学杂志 2024年第6期25卷 746-750页

作者：黄演婷陈小明曾丽婷杨小蓉黄兰兰广东药科大学附属第一医院检验科广东广州510062 广东药科大学临床医学院

目的了解广州市女性人乳头瘤病毒(human papilloma virus,HPV)的感染及基因分型情况,为宫颈癌防治提供流行病学依据。方法采集2021年1—12月广东药科大学附属第一医院门诊及体检的女性宫颈脱落细胞样本,利用聚合酶链式反应(polymerase c... 详细信息

目的了解广州市女性人乳头瘤病毒(human papilloma virus,HPV)的感染及基因分型情况,为宫颈癌防治提供流行病学依据。方法采集2021年1—12月广东药科大学附属第一医院门诊及体检的女性宫颈脱落细胞样本,利用聚合酶链式反应(polymerase chain reaction,PCR)-反向点杂交法,分析HPV基因型。结果调查5892名女性,HPV感染820例,感染率13.92%,其中HPV单一基因型感染637例(77.68%),双重感染140例(17.07%),多重感染43例(5.25%)。高危型HPV感染率(9.35%)高于低危型(3.00%)和混合感染型(1.56%)。高危型HPV感染率最高的前5种基因型依次是HPV52(3.60%)、HPV58(1.49%)、HPV16(1.46%)、HPV53(1.17%)和HPV51(0.88%),而低危型HPV感染率最高的前3种基因型依次为HPV44(1.17%)、HPV54(0.70%)和HPV61(0.68%)。各年龄组中,≥60岁组HPV感染率最高(29.34%),不同年龄组之间HPV的感染率差异有统计学意义(χ^(2)=47.44,P<0.05)。结论广州市女性HPV感染率较高,应加强女性预防HPV感染的意识,提高HPV的筛查和疫苗接种率。

关键词：人乳头瘤病毒感染率基因分型 PCR-反向点杂交法

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