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Journal of Genetics and Genomics 2011年第6期38卷 261-269页

作者： Hailong Liu Jing Han xiaoqing Liu Jian zhou Hua Xiang State Key Laboratory of Microbial Resources Institute of Microbiology Chinese Academy of Sciences Beijing 100101 China

The haloarchaea Haloferax mediterranei and Haloarcula hispanica are both polyhydroxyalkanoate producers in the domain Archaea, and they are becoming increasingly attractive for research and biotechnology due to their unique genetic and metabolic features. To accelerate their genome-level genetic and metabolic analyses, we have developed specific and highly efficient gene knockout systems for these two haloarchaea. These gene knockout systems consist of a suicide plasmid vector with the pyrF gene as the selection marker and a uracil auxotrophic haloarchaeon （apyrF） as the host. For in-frame deletion of a target gene, the suicide plasmid carrying the flanking region of the target gene was transferred into the corresponding apyrF host. After positive selection of the single-crossover integration recombinants （pop-in） on AS-168SY medium without uracil and counterselection of the double-crossover pyrF-excised recombinants （pop-out） with 5-fluoroorotic acid （5-FOA）, the target gene knockout mutants were confirmed by PCR and Southern blot analysis. We have demonstrated the effectiveness of these systems by knocking out the crtB gene which encodes a phytoene synthase in these haloarchaea. In conclusion, these well-developed knockout systems would greatly accelerate the functional genomic research of these halophilic archaea.

关键词： Knockout system pyrF Pop-in/pop-out method Haloarchaea

A videogrammetric technique for measuring the vibration displacement of stay cables

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Geo-Spatial Information Science 2012年第2期15卷 135-141页

作者： zhou xiaoqing XIA Yong WEI Zelong WU Qingxiong College of Civil Engineering Shenzhen University3688 Nanhai RoadShenzhen 518060China Department of Civil and Structural Engineering The Hong Kong Polytechnic UniversityHung HomKowloonHong KongChina College of Civil Engineering Fuzhou University2 Xue Yuan RoadUniversity TownFuzhou 350108China

Stay cables in cable-stayed bridges are prone to large amplitude oscillations under external *** vibration of the cables is predominantly measured by using accelerometers to measure the *** dynamic displacement is then usually obtained indirectly from the double integration of the acceleration *** paper reports an experimental method of measuring the displacement of stayed cables using a digital video *** the newly developed videogrammetric technique,the video clips are transferred into image frames,from which the shape and location of the target are *** displacement time history is then *** technique is applied to a cable-stayed bridge to measure the dynamic displacement of stay *** displacement is compared with the acceleration data in the frequency and time *** results show that the displacement measured by the digital video camera is comparable to the counterparts integrated from the acceleration *** vibration frequencies identified from the acceleration are finally used to estimate the tension forces of the *** results show that the tension forces have insignificant changes after one year’s operation.

关键词： stay cable vibration displacement measurement videogrammetric technique tension force

Numerical Calculation of Concrete Slab Response to Blast Loading

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Transactions of Tianjin University 2006年第B9期12卷 94-99页

作者： zhou xiaoqing HAO Hong KUZNETSOV Valerian A WASCHL John School of Civil and Resource Engineering The University of Western Australia Australia Weapons System Division Defence Science and Technology Organisation Department of Defence Australia

In the present paper, a dynamic plastic damage model for concrete has been employed to estimate responses of a reinforced concrete slab subjected to blast loading. The interaction between the blast wave and the concrete slab is considered in 3D simulation. In the first stage, the initial detonation and blast wave propagation is modelled in 2D simulation before the blast wave reaches the concrete slab, then the results obtained from 2D calculation are remapped to a 3D model. The calculated blast load is compared with that obtained from TM5-1300. Numerical results of the concrete slab response are compared with the explosive test carried out- in the Weapons System Division, Defence Science and Technology Organisation, Department of Defence, Australia.

关键词： blast loading damage concrete 3 D simulation

Study on the four Qi of Pfaffia glomerata based on the metabolomics technology and comparison of Dangshen(Radix Codonopsis) in the equivalent substitution prescription

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Journal of Traditional Chinese Medicine 2024年第4期44卷 713-721页

作者： SONG Zhenguang YANG Bin WANG Fei YAN Dongmei zhou xiaoqing HUANG Liping GAO Xuemei LI Bin HUANG Luqi Academician Workstation Jiangxi University of Chinese MedicineNanchang 330004China State Key Laboratory of Characteristic Chinese Medicine Resources in Southwest China School of PharmacyChengdu University of Traditional Chinese MedicineChengdu 610075China National Resource Center for Chinese Materia Medica Chinese Academy of Chinese Medical Sciences(CACMS)Beijing 100700China

OBJECTIVE:To explore the four Qi of Pfaffia glomerata(PG)and endow this foreign folk herb with the properties of Chinese medicine,make it Chinese medicinal and localized,and could be used as a Chinese ***:The normal group,six cold herb groups,six hot herb groups,six cool herb groups,PG prescription group(PGPG),and Dangshen(Radix Codonopsis)prescription group(CPPG)were prepared with corresponding concentrations of water extracts,these herb extracts were administered by gavage to SpragueDawley rats,and the 12 h urine at night on the 29th day of the SD rats in each group were collected,Liquid Chromatograph Mass Spectrometer system was used to analyze them,the best discriminant models for the medicinal properties of cold-hot and cold-cool were set up,so as to the medicinal properties of PGPG,CPPG and PG were *** on the Progenesis QI,Human Metabolome Database,Kyoto Encyclopedia of Genes and Genomes,MetaboAnalyst 5.0 database,we enriched metabolic pathway and classification mechanism of medicinal properties of cold-cool Chinese herbs and the molecular mechanism of PG ***:We established a best model of cold-hot herbal discrimination in the positive ion mode,then the probability that PGPG was predicted cold property was 88.9%.Furthermore,a model of cold-cool herbal discrimination was established,then the probability of PGPG containing the cool property was 77.8%.In addition,typical cold and cool herbs mainly affected nine biomarkers such as tyrosine-proline,(R)-3',7-Dihydroxy-2',4'-dimethoxyisoflavane in *** regulation trend of PGPG on markers was basically as same as the cool herbs and mainly involved in regulating the two pathways of cytochrome P450 and purine ***:The results showed PGPG had a cool medicinal property as same as CPPG,and the regulation trend of PGPG on markers was consistent with cool ***,the medicine properties PG and CP should be consistent,and the Traditional Chinese Medicine p

关键词： Pfaffia glomerata medicinal properties of Chinese medicine metabolomics molecular mechanism equivalent substitution

Kinematic inventory of rock glaciers in the Nyainqentanglha Range using the MT-InSAR method

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International Journal of Digital Earth 2023年第1期16卷 3923-3948页

作者： Xuefei Zhang Min Feng Jinhao Xu Dezhao Yan Jing Wang xiaoqing zhou Tao Li Xiang Zhang Land Satellite Remote Sensing Application Center Ministry of Natural ResourcesBeijingPeople’s Republic of China Tibetan Plateau Data Center State Key Laboratory of Tibetan Plateau Earth SystemEnvironment and Resources(TPESER)Institute of Tibetan Plateau ResearchChinese Academy of SciencesBeijingPeople’s Republic of China Academy of Plateau Science and Sustainability Qinghai Normal UniversityXiningPeople’s Republic of China University of Chinese Academy of Sciences BeijingPeople’s Republic of China Research Institute of Intelligent Computing Zhejiang LaboratoryHangzhouPeople’s Republic of China

Rock glaciers are typical periglacial landforms with tongue or lobate morphological shapes and characterized by the distinct front,lateral margins,and often by ridge-and-furrow surface topography textures as well as kinematic characteristics,widely distributed in alpine *** Synthetic aperture radar interferometry(MT-InSAR),is a remote sensing technique with demonstrated effectiveness for detecting landform ***,its application to rock glaciers is challenged by temporal decorrelation and atmospheric phase noises due to complex topography and snow *** designed a quadtree segmentation and parallel computing-based MT-InSAR method to improve the quality and efficiency of deformation measurement of rock *** applied the method to a rock glacier inventory of the Nyainqentanglha Range,China,derived from high-resolution Gaofen-2 images,to quantify the activity rate of each rock *** showed that 32.1%(6,389)of the identified rock glaciers exhibited slope-parallel deformation rates exceeding 100 mm/*** activities of the rock glaciers exhibited strong correlations with their distance to glaciers,precipitation,freeze-thaw magnitude,and permafrost occurrence *** results demonstrate the effectiveness of the developed segmentation-parallel MT-InSAR method for monitoring rock glacier deformation over a large region.

关键词： Rock glacier MT-InSAR quadtree segmentation Nyaingentanglha kinematics

Schisandrol A protects AGEs-induced neuronal cells death by allosterically targeting ATP6V0d1 subunit of V-ATPase

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Acta Pharmaceutica Sinica B 2022年第10期12卷 3843-3860页

作者： xiaoqing zhou Shaoyang Zhao Tingting Liu Lu Yao Meimei Zhao Xiaoming Ye Xiaowen Zhang Qiang Guo Pengfei Tu Kewu Zeng State Key Laboratory of Natural and Biomimetic Drugs School of Pharmaceutical SciencesPeking UniversityBeijing 100191China

Diabetes have been shown to cause progressive neuronal injury with pain and numbness via advanced glycation end-products(AGEs)-induced neuronal cell apoptosis;however, the valuable drug targets for diabetic neuropathy have been poorly reported so far. In this study, we discovered a natural small-molecule schisandrol A(SolA) with significant protective effect against AGEs-induced neuronal cell apoptosis. ATP6V0D1, a major subunit of vacuolar-type ATPase(V-ATPase) in lysosome was identified as a crucial cellular target of SolA. Moreover, SolA allosterically mediated ATP6V0D1 conformation via targeting a unique cysteine 335 residue to activate V-ATPase-dependent lysosomal ***, SolA-induced lysosome pH downregulation resulted in a mitochondrial-lysosomal crosstalk by selectively promoting mitochondrial BH3-only protein BIM degradation, thereby preserving mitochondrial homeostasis and neuronal cells survival. Collectively, our findings reveal ATP6V0D1 is a valuable pharmacological target for diabetes-associated neuronal injury via controlling lysosomal acidification, and also provide the first small-molecule template allosterically activating V-ATPase for preventing diabetic neuropathy.

关键词： Allosteric regulation Advanced glycation endproducts AGEs Lysosomal acidification Targets V-ATPase ATP6V0D1 Schisandrol A Diabetic neuropathy

Transcranial Magneto-Acoustic Stimulation Attenuates Synaptic Plasticity Impairment through the Activation of Piezo1 in Alzheimer’s Disease Mouse Model

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Research 2024年第1期2023卷 81-95页

作者： Fangxuan Chu Ruxin Tan Xin Wang xiaoqing zhou Ren Ma Xiaoxu Ma Ying Li Ruixu Liu Chunlan Zhang Xu Liu Tao Yin Zhipeng Liu Institute of Biomedical Engineering Chinese Academy of Medical Sciences and Peking Union Medical CollegeTianjin300192China Tianjin Institutes of Health Science Tianjin301600China Neuroscience Center Chinese Academy of Medical Science&Peking Union Medical CollegeBeijing100730China

The neuropathological features of Alzheimer’s disease include amyloid *** emerging evidence suggests that Piezo1,a mechanosensitive cation channel,plays a critical role in transforming ultrasound-related mechanical stimuli through its trimeric propeller-like structure,but the importance of Piezo1-mediated mechanotransduction in brain functions is less ***,apart from mechanical stimulation,Piezo1 channels are strongly modulated by *** assume that Piezo1 may play a role in converting mechanical and electrical signals,which could induce the phagocytosis and degradation of Aβ,and the combined effect of mechanical and electrical stimulation is superior to single mechanical ***,we design a transcranial magneto-acoustic stimulation(TMAS)system,based on transcranial ultrasound stimulation(TUS)within a magnetic field that combines a magnetoacoustic coupling effect electric field and the mechanical force of ultrasound,and applied it to test the above hypothesis in 5xFAD *** tests,in vivo electrophysiological recordings,Golgi–Cox staining,enzyme-linked immunosorbent assay,immunofluorescence,immunohistochemistry,real-time quantitative PCR,Western blotting,RNA sequencing,and cerebral blood flow monitoring were used to assess whether TMAS can alleviate the symptoms of AD mouse model by activating Piezo1.

关键词： stimulation Alzheimer Piezo1

NUMERICAL COMPUTATION OF TWO-PHASE TURBULENT COMBUSTION PROCESSES

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Chinese Journal of Aeronautics 1988年第1期 27-33页

作者： zhou xiaoqing Shenyang Aeroengine Research Institute

A self-compiled computer code GEMCHIP is briefly *** of this method to several typicalcombustor flows is *** Design and Development of air-breathing

关键词： NUMERICAL COMPUTATION OF TWO-PHASE TURBULENT COMBUSTION PROCESSES UI

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母液对磁流变液力学性质的影响

应用力学学报 2007年第2期24卷 223-226页

作者：周小清邬云文吉首大学湖南吉首416000

研究了磁流变液中母液分子在磁场作用下磁矩的变化以及由此引起的剪切屈服强度的变化,探讨了磁流变的微观机理。研究表明,由分子磁矩引起的剪切屈服强度与磁流变液总屈服强度的比随母液分子内电子数的增加而增加,一般情况下,单位体积内... 详细信息

研究了磁流变液中母液分子在磁场作用下磁矩的变化以及由此引起的剪切屈服强度的变化,探讨了磁流变的微观机理。研究表明,由分子磁矩引起的剪切屈服强度与磁流变液总屈服强度的比随母液分子内电子数的增加而增加,一般情况下,单位体积内的平均分子磁矩占总磁矩的2·53%,由此引起的剪切屈服强度的减少占总剪切屈服强度的5·15%。为减少误差,可尽量选用电子数较少的分子材料作母液。

关键词：磁流变液分子磁矩剪切屈服强度

Numerical Modeling of Response and Damage of Masonry Walls to Blast Loading

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Transactions of Tianjin University 2006年第B9期12卷 132-137页

作者： DEEKS Andrew School of Civil and Resource Engineering The University of Western AustraliaAustralia

To model the damage process of masonry walls under blast loading, a dynamic continuum damage material model is constructed for brick and mortar separately. The degradation of both the stiffness and strength are governed by a damage variable. By using the proposed material model, damage and fragmentation of a typical masonry wall under blast loading at different scaled distances is calculated. The hazard level of the masonry wall to blast loading is evaluated by analyzing the numerical results.

关键词： masonry blast loading fragmentation hazard level damage numerical modeling