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Journal of Forestry Research 2019年第5期30卷 1793-1804页

作者： Dandan Li xiaoqing hu Chenghao Li State Key Laboratory of Tree Genetics and Breeding Northeast Forestry University26 Hexing RoadHarbin 150040People’s Republic of China College of Life Science Northeast Forestry UniversityHarbin 150040People’s Republic of China

Proanthocyanidins(PAs)are the most broadly distributed secondary metabolites that play important roles in various aspects of plant development and response to biotic and abiotic *** this study,we cloned a R2R3 MYB gene LoMYB29,which has a full-length coding sequence of 921 bp identified in Larix *** real-time reverse transcription polymerase chain reaction analysis indicates that LoMYB29 is expressed under mechanical wounding,high light intensity,and NaCl,PEG6000,Methyl Jasmonate,and abscisic acid *** localization analysis and yeast twohybrid assay localized LoMYB29 to the nucleus,acting as a transcriptional *** with 4-dimethylaminocinnamaldehyde showed a darker blue-purple color in LoMYB29-overexpressing Arabidopsis seeds compared to that of wild ***29-overexpression resulted in a significant increase in leaf PA *** expression of early flavonoid biosynthesis-related gene CHI and late flavonoid biosynthesis-related genes,including DFR,LDOX,and ANR(PA branch gene),were also activated in transgenic plants overexpressing *** results indicate that LoMYB29 plays a positive role in the regulation of PA biosynthesis by activating the expression of PA biosynthetic genes.

关键词： Olga Bay larch MYB transcription factors TT2 Proanthocyanidin

Multi-objective optimization sensor node scheduling for target tracking in wireless sensor network

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High Technology Letters 2014年第3期20卷 267-273页

作者：文莎 Cai Zixing hu xiaoqing School of Information Science and Engineering Central South University School of Automation Science and Engineering South China University of Technology

Target tracking in wireless sensor network usually schedules a subset of sensor nodes to constitute a tasking cluster to collaboratively track a *** the goals of saving energy consumption,prolonging network lifetime and improving tracking accuracy,sensor node scheduling for target tracking is indeed a multi-objective optimization *** this paper,a multi-objective optimization sensor node scheduling algorithm is *** employs the unscented Kalman filtering algorithm for target state estimation and establishes tracking accuracy index,predicts the energy consumption of candidate sensor nodes,analyzes the relationship between network lifetime and remaining energy balance so as to construct energy efficiency *** results show that,compared with the existing sensor node scheduling,our proposed algorithm can achieve superior tracking accuracy and energy efficiency.

关键词： wireless sensor network (WSN) target tracking sensor scheduling multi-objective optimization

Improvement of lycopene biosynthesis in waaC and waaF mutants of Escherichia coli by integrant expression of crtEBI gene and deletion of aceE and gdhA

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Systems Microbiology and Biomanufacturing 2023年第4期3卷 739-749页

作者： xiaoqing hu Mao Cui Xiaoyuan Wang State Key Laboratory of Food Science and Technology Jiangnan UniversityWuxi 214122China International Joint Laboratory on Food Safety Jiangnan UniversityWuxi 214122China

As a bright red carotenoid pigment found in tomatoes and other red fruits,lycopene was efficiently produced by Escherichia coli transformed with the genes involving in lycopene *** previous work showed that ***ΔwaaC andΔwaaF with higher permeability of outer membrane were better chassis for lycopene ***,further work needed to improve lycopene synthesis in the aforementioned *** the current study,the exogenous crtEBI genes from *** 14067 were first integrated into the chromosome ofΔwaaC andΔ*** toΔwaaC/pWSK29-crtEBI andΔwaaF/pWSK29-crtEBI(4.19 and 4.20 mg/g lycopene respectively),ΔwaaC lacZ::crtEBI(CWC01)andΔwaaF lacZ::crtEBI(CWF01)produced higher lycopene levels at 16.14 mg/g and 15.81 mg/g,***,the individual and combinational deletion of aceE and gdhA was conducted in CWC01 and CWF01,*** double knock-out strains CWC04 and CWF04 showed the improved yield at 19.65 mg/g and 22.24 mg/g,***,the four genes involving in MEP pathway(dxs,dxr,ispA and idi)were further *** highest lycopene yield was achieved at 25.82 mg/g by CWF04/pACYC184-dxs^(E289G)-dxr^(K37N,K217N)-*** current study showed that ***ΔwaaC andΔwaaF were optimal chassis for ambitious metabolic modification to produce lycopene.

关键词： Lycopene Escherichia coli waaC waaF

The Association between Physical Education and Mental Health Indicators in Adolescents:A Cross-Sectional Study

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International Journal of Mental Health Promotion 2022年第5期24卷 783-793页

作者： xiaoqing hu Yan Tang School of Physical Education and Sports Training Shanghai University of SportShanghai200438China Shanghai Research Centre for Physical Fitness and Health of Children and Adolescents Shanghai University of SportShanghai200438China

Objective:To explore the associations between physical education attendance and mental health ***:Using data from the Global Student Health Survey,the frequency of physical education attendance,suicidality-related indicators,loneliness,bullying,and anxiety were all assessed using a standardized self-reported *** logistic regression was used to estimate the association between physical education attendance and mental health-related ***:The study included 276,169 participants from 71 coun-tries(47.3%males,aged 11–18 years old).After controlling for sex,age,food insecurity,close friends,physical activity,sedentary time,others’help,and parents’understanding,physical education attendance was not signiﬁ-cantly associated with suicidal attempts,suicidal ideation,and ***,compared with no physical edu-cation attendance,individuals attending physical education for 1,2,as well as 5 or more days had signiﬁcantly reduced odds/ratios of suicide;only 2 days of physical education attendance was associated with a lower odds ratio for suffering from *** 1 day of physical education was associated with not being bullied by ***:This study suggests that physical education attendance may not have an effective role in reducing mental health illnesses in children and *** studies are encouraged to corroborate or negate our research discoveries by using better and further improved study designs.

关键词： Physical education mental illness loneliness suicidality bullying behaviors

Improve gamma‑aminobutyric acid production in Corynebacterium glutamicum by optimizing the metabolic fux

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Systems Microbiology and Biomanufacturing 2022年第2期2卷 305-316页

作者： Chengzhen Yao Yuantao Liu xiaoqing hu Xiaoyuan Wang State Key Laboratory of Food Science and Technology Jiangnan UniversityWuxi 214122China International Joint Laboratory on Food Safety Jiangnan UniversityWuxiJiangsuChina Key Laboratory of Industrial Biotechnology Ministry of EducationSchool of BiotechnologyJiangnan UniversityWuxiChina hulunbeier Northeast Fufeng Biotechnology Co.Ltd. HulunbeierChina

Gamma-aminobutyric acid is an important nonprotein amino acid and has been extensively applied in pharmaceuticals,livestock,food additives,and so *** is important to develop Corynebacterium glutamicum strains that can efciently produce gamma-aminobutyric acid from *** this study,production of gamma-aminobutyric acid in *** CGY700 was improved by construction of CO_(2) anaplerotic reaction and overexpression of citrate *** co-expression of ppc encoding phosphoenolpyruvate carboxylase and gltA encoding citrate synthase was constructed and optimized in the chromosome to compensate carbon loss and conquer metabolic *** expression of ppc and gltA were controlled by promoters P_(tac) and P_(tacM),and the optimal mode of P_(tacM-ppc)-P_(tac)-gltA was ***,the genes pknG encoding serine/threonine protein kinase G and ldh encoding l-lactate dehydrogenase were deleted,and glnA2 encoding glutamine synthase was overexpressed in the *** fnal strain CGY-PG-304 constructed in this study could produce 41.17 g/L gamma-aminobutyric acid in shake fask cultivation and 58.33 g/L gamma-aminobutyric acid via FedBatch fermentation with a yield of 0.30 g/g ***-PG-304 was constructed by genome editing;therefore,it is stable and not necessary to add any antibiotics and inducer during fermentation.

关键词： Gamma-aminobutyric acid Metabolic engineering Corynebacterium glutamicum Phosphoenolpyruvate carboxylase Citrate synthase

Complex regulatory network of Betula BplSPL8 in planta

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Journal of Forestry Research 2017年第5期28卷 881-890页

作者： Chuang Liu Minxiao Guan xiaoqing hu Jing Tian Xuemei Liu College of Life Science Northeast Forestry University Suzhou Micro Diag Biomedicine Co. LtdJiangsu Engineering Research Center for Tumor Molecular Diagnostics

SQUAMOSA-promoter binding protein-like (SPL) proteins are plant-specific transcription factors and participate in different pathways, including the vegetative to reproductive transition, male sterility, biosynthesis of gibberellic acid (GA), plant morphogenesis and response to environmental stress. In this study, we generated transgenic Arabidopsis that overexpressed Betula BplSPL8 and confirmed that BplSPL8 is a transcription factor with transcriptional activation activity and is located in the nucleus. Functional analysis of BplSPL8 showed that it is involved in regulating different development processes: (1) BplSPL8 can delay flowering by reducing sensitivity to GA under short days; (2) BplSPL8 controls the number and morphogenesis of leaves, including up-rolling leaves under long days and folded leaves mediated by GA under short days; (3) BplSPL8 can promote root elongation during late development of roots and inhibit lateral root formation; (4) BplSPL8 may be involved in regulating carotenoid biosynthesis and secretion metabolism. These results show that there is a complex regulatory network for the SPL family genes that is mediated by other components and may provide a new insights for the functional research of SPL genes.

关键词： Birch Root SQUAMOSA-promoter binding protein-like Transgenic Carotenoid

Deep-water Depositional Features of Miocene Zhujiang Formation in Baiyun Sag,Pearl River Mouth Basin

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Acta Geologica Sinica(English Edition) 2013年第1期87卷 197-210页

作者： LI Yun ZHENG Rongcai YANG Baoquan Zhu Guojin GAO Boyu hu xiaoqing State Key Laboratory of Oil and Gas Reservoir Geology and Exploitation Chengdu University of Technology China National Offshore Oil Corporation Research Center

Eleven lithofacies and five lithofacies associations were indentified in the Miocene Zhujiang Formation on the basis of detailed core analysis. It could he determined that three depositional types developed, namely submarine fan, basin and deep-water traction current. Six microfacies were further recognized within the fan, including main channels in the inner fan, distributary channels in the middle fan, inter-channels, levees and the outer fan. The lower Zhujiang Formation, mainly sandstone associations, was inner fan and inner-middle fan deposits of the basin fan and the slope fan. The middle part, mainly mudstone associations, was outer fan deposits. With the transgression, the submarine fan was finally replaced by the basinal pelagic deposits which were dominated by mudstone associations, siltstone associations, and deep-water limestone associations. During the weak gravity flow activity, the lower channels, the middle-upper outer fans and basin deposits were strongly modified by the deep-water traction current. The identification of the deep-water traction deposition in Miocene Zhujiang Formation would be of great importance. It could be inferred that the deep- water traction current had been existing after the shelf-break formation since the Late Oligocene （23.8 Ma） in the Baiyun sag, influencing and controlling the sediment composition, the distribution, and depositional processes. It would provide great enlightenment to the paleo-oceanic current circulation in the northern South China Sea.

关键词： northern South China Sea Pearl River Mouth Basin Baiyun Sag Zhujiang Formation,submarine fan

Biosynthesis and transfer of 2‑acetamido‑4‑methylamino‑l‑fucose to the core‑lipid A in Bordetella pertussis

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Systems Microbiology and Biomanufacturing 2023年第2期3卷 273-286页

作者： Liangjia Wang Zhen Wang Shuaiwen Wang xiaoqing hu Xiaoyuan Wang State Key Laboratory of Food Science and Technology Jiangnan University1800 Lihu AvenueWuxi 214122China International Joint Laboratory on Food Safety Jiangnan UniversityWuxi 214122China Key Laboratory of Industrial Biotechnology Ministry of EducationSchool of BiotechnologyJiangnan UniversityWuxi 214122China

The trisaccharide unit in lipopolysaccharide of Bordetella pertussis is important for immune recognition,but how the rare sugar 2-acetamido-4-methylamino-fucose in the trisaccharide unit is synthesized and transferred to the core of lipopolysaccharide in *** remains *** this study,we demonstrated that the genes bplF,bplG,bplL and bplI are involved in the synthesis and transfer of 2-acetamido-4-methylamino-fucose to the core of lipopolysaccharide in *** genes were overexpressed with various combination in a recombinant *** strain which can synthesize *** corelipid *** were isolated from these strains and analyzed by using SDS-PAGE,ion chromatography and *** on these analyses,the mechanism of biosynthesis and transfer of 2-acetamido-4-methylamino-l-fucose to the core-lipid A in *** is proposed.

关键词： Bordetella pertussis Escherichia coli Lipopolysaccharide Trisaccharide unit 2-Acetamido-4-methylamino-L-fucose BplL

Differentiating electrophysiological response to decrease and increase negative emotion regulation

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Chinese Science Bulletin 2013年第13期58卷 1543-1550页

作者： WU HaiYan TANG Ping huANG XuShu hu xiaoqing LUO YueJia State Key Laboratory of Cognitive Neuroscience and Learning Beijing Normal University Sichuan Research Center of Applied Psychology Chengdu Medical College Department of Psychology Northwestern University

We investigated the reappraisal and the time course of negative emotion regulation by performing event-related potential (ERP) recordings. We found that negative pictures elicited more positive P2 and late positivity potential (LPP) deflections than neutral pictures. This effect occurred between 150-2000 ms post-stimulus. Compared to the emotion maintaining condition, the emotion enhancing condition was associated with higher arousal ratings and displayed increased P2 and LPP amplitudes. The decrease condition was also associated with reduced picture-induced arousal; however, it led to increased P2 and LPP amplitudes. Furthermore, when compared with the maintain condition, both the enhancing and decrease conditions significantly enhanced LPP in the early stage (350-750 ms). Compared to previous studies using western subjects, the negative emotion LPP effects of the present study were shorter in duration and the decrease-emotion condition elicited larger LPPs.

关键词：情绪调节电生理反应负性事件相关电位 LPP 时间过程早期阶段持续时间

Diagnosis of sewer pipe defects on image recognition of multi-features and support vector machine in a southern Chinese city

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Frontiers of Environmental Science & Engineering 2019年第2期13卷 29-41页

作者： Xiangyang Ye Jian’e Zuo Ruohan Li Yajiao Wang Lili Gan Zhonghan Yu xiaoqing hu State Key Joint Laboratory of Environment Simulation and Pollution Control School of EnvironmentTsinghua UniversityBeijing 100084China Thunip Corp. Ltd.27F/Section CS&T BuildingTsinghua S&T ParkBeijing 100084China China Water Environment Group.10F Campbell Building89th Jinbao StreetBeijing 101101China

Closed circuit television(CCTV)systems are widely used to inspect sewer pipe *** the diagnosis process,the manual diagnosis of defects is time consuming,labor intensive and error *** assist inspectors in diagnosing sewer pipe defects on CCTV inspection images,this paper presents an image recognition algorithm that applies features extraction and machine learning *** algorithm of image recognition techniques,including hu invariant moment,texture features,lateral Fourier transform and Daubechies(DBn)wavelet transform,was used to describe the features of defects,and support vector machines were used to classify sewer pipe *** to the inspection results,seven defects were defined;the diagnostic system was applied to a sewer pipe system in a southern city of China,and 28,760 m of sewer pipes were *** results revealed that the classification accuracies of the different defects ranged from 51.6% to 99.3%.The overall accuracy reached 84.1%.The diagnosing accuracy depended on the number of the training samples,and four fitting curves were applied to fit the *** to this paper,the logarithmic fitting curve presents the highest coefficient of determination of 0.882,and more than 200 images need to be used for training samples to guarantee the accuracy higher than 85%.

关键词： Sewer pipe defects Defect diagnosing Image recognition Multi-features extraction Support vector machine