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Lead acetate in drinking water is toxic to hippocampal tissue Measuring relative protein changes using tissue array detection

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Neural Regeneration Research 2010年第7期5卷 519-524页

作者： xiaoqin ha Qiang Yin Tongde Lue Bin Liu Yuebin Xu Chunjie Liu Xiaohui Yu Center for Medical Experiment Lanzhou General Hospital of Lanzhou Military Area Command of Chinese PLA Lanzhou 730050 Gansu Province China School of Chemical Engineering and Technology Tianjin University Tianjin 300072 China

BACKGROUND： Lead can cause structural changes in the hippocampus, followed by damage to learning and memory functions, but its specific mechanisms are not yet clear. OBJECTIVE： To observe long-term toxicity of high-dose lead in drinking water on hippocampal tissue in rats, and analyze the potential association of oxidative damage, cell apoptosis, and pathology. DESIGN, TIME AND SETTING： A randomized, controlled animal experiment was performed at the Center for Medical Experiment, Lanzhou General Hospital of Lanzhou Military Area Command of Chinese PLA from May 2007 to October 2008. MATERIALS; Rabbit anti Bcl-2, Bax, and inducible nitric oxide synthase （iNOS） polyclonal antibodies were purchased from Santa Cruz Biotechnology, USA. An streptavidin-peroxidase immunohistochemistry kit and concentrated DAB kit were purchased from Beijing Zhongshan Biotechnology Company Limited, China. Crystal violet was purchased from Sigma, USA. METHODS： A total of 72 Wistar rats, aged 3 months, were randomly divided into control, low-, middle-, and high-dose lead groups, with 18 rats per group. Animal models were established through free drinking water contaminated by Pb2＋ for 1, 3, and 6 months, respectively. MAIN OUTCOME MEASURES： The general toxicity of lead was dynamically observed; the levels of Pb2＋ in the blood and brain tissue homogenete were detected using atomic absorption method; pathological changes were observed using hematoxylin-eosin staining and tigroid body staining; the protein expression levels of Bcl-2, Bax, and iNOS were dynamically observed using streptavidin-peroxidase immunohistochemistry of the hippocampus. RESULTS： Lead exposure reduced autonomic activities, produced a slumped appearance, slow responses, and lusterless fur, especially in the high-dose group. The amount of ingestion and hydroposia showed a decreasing trend, especially in middle- and high-dose groups. Lead levels in whole blood and brain homogenate were higher than controls （P 〈 0.01）. Lead caus

关键词： lead exposure hippocampus injury tissue array apoptosis neurotoxicity

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Therapeutic angiogenesis induced by human hepatocyte growth factor gene in hindlimb ischemia of dogs

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Chinese Science Bulletin 2003年第7期48卷 676-680页

作者： ha xiaoqin, REN Jianping, BI Jianjin, ZhaNG Jinhui & WU Zuze (Wu Chu-tse) Beijing Institute of Radiation Medicine, Beijing 100850, China 1. Beijing Institute of Radiation Medicine Beijing 100850 China

A preclinical study of treating peripheral ar-tery occlusive disease (PAD) was performed by using ahepatocyte growth factor (HGF) gene-expressing vector,plasmid pUDKH, in a dog model with complete ischemia of one hindlimb. After ligation of femoral artery of onehindlimb, pUDKH was transferred directly into the ischemic limb muscles. The angiogenic activity of the plasmid pUDKH was evaluated. On D 30 after injecting once of pUDKH at different doses into local muscles immediately after opera-tion, the degree of augmentation of collateral vessel forma-tion was significantly greater than that treated by blankvector. In addition, the blood flow rate of femoral artery in dogs treated with pUDKH was recovered on D 90, while the flow rate was only 1/5 to 1/3 in control dogs. The pulse am-plitude of pUDKH-treated dogs was recovered on D 90, but it was hardly detectable in most of the control dogs. The sideeffects of intramuscular transfection of pUDKH were alsoinvestigated, and no significant positive change was found. It is suggested that angiogenesis induced by HGF gene has the potential for clinical use in the treatment of peripheral arte-rial diseases.

关键词：外周动脉闭塞疾病肝细胞生长因子基因治疗动物模型下肢局部缺血血管生成

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Hepatocyte growth factor gene transfer effects on the femoral and intramuscular nerve in a canine model of lower limb ischemia

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Neural Regeneration Research 2008年第6期3卷 625-628页

作者： xiaoqin ha Bin Liu Zhen Qian Tongde LU Ling Hui Guanxian He Qiang Yin Tingxian Niu Medical Experimental Center Lanzhou General Hospital of Lanzhou Military Area Command of Chinese PLA Lanzhou 730050 Gansu Province China Department of Pathology Lanzhou General Hospital of Lanzhou Military Area Command of Chinese PLA Lanzhou 730050 Gansu Province China Animal Experimental Department Lanzhou General Hospital of Lanzhou Military Area Command of Chinese PLA Lanzhou 730050 Gansu Province China 不详

BACKGROUND： Recent advancements in gene therapy have provided new methodology for treating ischemia in lower extremities. Gene transfer of angiogenic factors to ischemic tissues may promote local proliferation of new vessels and form collateral circulation. OBJECTIVE： To observe histopathological changes in the femoral and intramuscular nerve three months after intramuscular injection of hepatocyte growth factor （HGF） into the peripheral skeletal muscle in a canine model of lower limb ischemia. DESIGN： Randomized occlusion modelled and verification animal study. SETTING： Experimental Center, Lanzhou General Hospital of Lanzhou Military Area Command of Chinese PLA. MATERIALS： This study was performed at Animal Experimental Center, Lanzhou General Hospital of Lanzhou Military Area Command of Chinese PLA from September to November 2006. A total of eight male mongrel dogs, weighing 12–15 kg and 1.5–3 years of age, were selected for this study. This experimental study was in accordance with local ethics standards. Recombinant plasmid carrying HGF （pUDKH） and occlusion model plasmid （pUDK） were provided by the Third Laboratory of Radiation Medical Institute, Academy of Military Medical Sciences of PLA. METHODS： Grouping and model establishment： under anesthesia, complete vascular occlusion models were established on the left lower extremities. The experimental dogs were randomly divided into a model group and a pUDKH treatment group, with four dogs in each group. Dogs in the pUDKH group were injected with 0.15 mg/kg pUDKH. Ten minutes later, intramuscular injections were performed at three spots into the peripheral skeletal muscle of the left hind limb, as well as lateral injections at two spots. The injection volume at each spot was 0.2 mL. Dogs in the model group were injected with pUDK, and dosage and injection method were identical to the treatment group. MAIN OUTCOME MEASURES： Histopathological changes in the femoral nerve, as well as internal and external intramuscu

关键词： hematopoietic reconstitution hepatocyte growth factor histopathology lower limb ischemia nerve recombinant plasmid

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Biological effect of NK4 gene mediated by attenuated Salmonella typhimurium on hepatocellular carcinoma cell HepG2

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The Chinese-German Journal of Clinical Oncology 2009年第12期8卷 688-693页

作者： xiaoqin ha Ruifang Fan Tongde Lv Yuebin Xu Ling Hui Xiaopeng Yang Qinhua Jia Medical Experiment Center Lanzhou General Hospital of Lanzhou Military Region People＇s Liberation ArmyLanzhou 730050 China Department of Hepatobiliary Surgery Lanzhou General Hospital of Lanzhou Military Region People＇s LiberationArmy Lanzhou 730050 China

Objective： The aim of the study was to construct a stable strain of recombined attenuated Salmonella typhimurium expressing NK4 gene, and observe the effect of the strain on the metastatic potentiality of HepG2 cells. Methods： The NK4 cDNA was isolated from PCAGGS/hNK4 plasmid by PCR, and subcloned into eukaryotic expression vector pcDNA4. The recombinant plasmid was electro-transferred into attenuated Salmonella typhimurium Ty21a to obtain the recombinant strain encoding NK4 gene （TPN）. Simultaneously, the recombinant attenuated Salmonella typhimurium carrying GFP gene （TPG） was also constructed. After the TPG and TPN were transferred into HepG2 cells, the transfection rate and the expression level of NK4 protein were detected by flow cytometry and ELISA, and the effects of expression product on the proliferation and migration of HepG2 and angiogenesis were observed. Results： The TPN and TPG were successfully constructed. Fortyeight hours after transfection with TPG, the infection rate was 82.58% ± 1.74%, and the expression level of NK4 protein in supernatant was （181.5 ± 11.7） ng/6 × 10^5 cells. The supematant had obviously depressant effect on the proliferative activity of HepG2 cells （P 〈 0.05）, and could obviously restrain the hepatocyte growth factor-mediated migration of tumor cells （P 〈 0.01）. The inhibitory effect of the expression product on the tumor angiopoiesis was obviously observed （P 〈 0.05）, without a dosage-effect relation. Conclusion： The TPN could effectively transfer tumor cells in vitro and express interest NK4 protein. The expression product could effectively inhibit the proliferation and migration of hepatocellular carcinoma cells and the tumor angiopoiesis.

关键词： NK4 gene Salmonella typhimurium hepatocellular carcinoma angiopoiesis proliferation migration hepatocyte growth factor (HGF)

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胰岛素样生长因子2 mRNA结合蛋白2基因与2型糖尿病相关性的研究进展

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中国生物制品学杂志 2023年第3期36卷 363-367页

作者：冯珊珊哈小琴甘肃中医药大学公共卫生学院甘肃兰州730000 中国人民解放军联勤保障部队第九四〇医院甘肃兰州730000

2型糖尿病(type 2 diabetes mellitus,T2DM)是一种多基因遗传和环境因素共同作用的慢性、非传染性疾病,其主要特征包括胰岛素抵抗和胰岛素分泌不足。胰岛素样生长因子2 mRNA结合蛋白2(insulin-like growth factor 2 mRNA binding protei... 详细信息

2型糖尿病(type 2 diabetes mellitus,T2DM)是一种多基因遗传和环境因素共同作用的慢性、非传染性疾病,其主要特征包括胰岛素抵抗和胰岛素分泌不足。胰岛素样生长因子2 mRNA结合蛋白2(insulin-like growth factor 2 mRNA binding protein 2,IGF2BP2/IMP2)作为机体一种重要的胰岛素分泌相关蛋白,其主要在胰腺、脂肪和肠道等组织细胞中表达,IGF2BP2被证实可下调IGF2的表达,与其有关的胰岛β细胞功能破坏是T2DM及血管并发症的重要原因,因此,IGF2BP2基因可作为预测糖尿病风险的重要因子之一。本文就IGF2BP2基因与T2DM之间的相关性作一综述。

关键词：胰岛素样生长因子2 mRNA结合蛋白2 2型糖尿病相关性

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老年小鼠踝关节软骨细胞体外分离及培养鉴定

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中国组织工程研究 2022年第32期26卷 5097-5101页

作者：贾彩霞何斯哈小琴兰州大学公共卫生学院甘肃省兰州市730000 中国人民解放军联勤保障部队第九四〇医院甘肃省兰州市730050 甘肃省干细胞与基因药物重点实验室甘肃省兰州市730050 兰州大学第二临床医学院甘肃省兰州市730000

背景:老年小鼠的关节软骨本身存在一定的磨损,提取难度较大;尤其是踝关节等难以分离关节软骨面的小关节软骨细胞的提取方法更加少见。目的:探究实验室改良两步酶消化法提取老年小鼠踝关节软骨细胞的效果,体外培养观察并评价其生物学特... 详细信息

背景:老年小鼠的关节软骨本身存在一定的磨损,提取难度较大;尤其是踝关节等难以分离关节软骨面的小关节软骨细胞的提取方法更加少见。目的:探究实验室改良两步酶消化法提取老年小鼠踝关节软骨细胞的效果,体外培养观察并评价其生物学特性。方法:选取46周龄老年BALB/c雄性小鼠踝关节,通过实验室改良的0.25%胰酶EDTA、2 g/LⅡ型胶原酶两步酶消化法顺序进行分离提取及体外培养。使用倒置显微镜观察细胞生长形态,锥虫蓝染色并计数原代贴壁细胞,甲苯胺蓝和Ⅱ型胶原免疫荧光染色,二甲基亚甲基蓝显色法测定原代贴壁细胞培养液和消化液中硫酸糖胺多糖水平,CCK-8试剂盒检测软骨细胞增殖进行软骨细胞生物学特性鉴定及评价。结果与结论:①关节软骨经两步酶消化后,细胞全部解离释放,老年小鼠每个踝关节平均收获9×10^(5)个原代贴壁细胞;原代及第1代、第2代细胞贴壁形态为梭形和多角形;②细胞甲苯胺蓝染色可见蓝紫色易染颗粒,Ⅱ型胶原免疫荧光染色可见红色荧光阳性表达;③原代细胞培养液和消化液中硫酸糖胺多糖分别为(42.41±15.00),(65.63±10.00)mg/L;阴性对照中硫酸糖胺多糖分别为(0.35±3.78),(0.21±2.56)mg/L;④原代细胞第6天细胞数量达到最大,第1代和第2代细胞第5天达到最大,同一时间点,第1代细胞的细胞活力大于第2代细胞和原代细胞;⑤结果证实:实验室改良的两步酶消化法可成功分离老年小鼠踝关节软骨细胞,贴壁细胞纯度高、数量多,可成功合成和分泌Ⅱ型胶原及蛋白聚糖,具有良好的生物学特性,其中第1代软骨细胞的活力最高,第2代次之,可用于实验研究。

关键词：老年小鼠踝关节软骨细胞酶消化法细胞培养

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将内皮祖细胞纳入心血管疾病治疗方案的争论与探讨

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临床心血管病杂志 2016年第10期32卷 1055-1059页

作者：徐倩曹荟哲哈小琴兰州大学第二附属医院兰州730030 兰州理工大学兰州军区兰州总医院检验科

内皮祖细胞(EPCs)对受损血管的修复和新血管的形成至关重要。机体缺血或血管损伤时,EPCs可直接分化为成熟的内皮细胞(ECs)或分泌多种细胞因子促进血管的修复和新生。心血管疾病(CVD)的发生与体内EPCs的数量和功能密切相关,有建议认为可... 详细信息

内皮祖细胞(EPCs)对受损血管的修复和新血管的形成至关重要。机体缺血或血管损伤时,EPCs可直接分化为成熟的内皮细胞(ECs)或分泌多种细胞因子促进血管的修复和新生。心血管疾病(CVD)的发生与体内EPCs的数量和功能密切相关,有建议认为可将其作为CVD的临床生物标志物。然而,目前关于EPCs的定义及其鉴定方法尚未标准化,因此关于将其作为CVD标志物值得探讨。

关键词：内皮祖细胞内皮细胞心血管疾病血管损伤

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益生菌、益生元对慢性肾脏疾病治疗的研究进展

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中国微生态学杂志 2018年第5期30卷 608-612页

作者：杨波哈小琴中国人民解放军兰州总医院检验科甘肃兰州730050

益生菌是近些年来的研究热点,其对人体的有益作用越来越被关注,其在治疗众多疾病上有显著效果,本文将分析和总结益生菌对慢性肾脏疾病(chronic kidney disease,CKD)方面的重要影响。益生菌通过黏液层、上皮层、肠相关淋巴组织这三个层... 详细信息

益生菌是近些年来的研究热点,其对人体的有益作用越来越被关注,其在治疗众多疾病上有显著效果,本文将分析和总结益生菌对慢性肾脏疾病(chronic kidney disease,CKD)方面的重要影响。益生菌通过黏液层、上皮层、肠相关淋巴组织这三个层次对肠道进行作用,并且增加黏液和上皮细胞紧密连接以及上皮细胞的存活力来增强肠道屏障,而且又可以发挥营养作用。在其对CKD的影响中,益生菌、益生元和合生元可以降低尿毒症毒素,也可以减少免疫炎症的反应,提高肾功能和生活质量。益生菌组合剂量、益生菌与益生元组合方式都会影响益生菌制剂的效果,并且个体肠道的差异以及抗生素的使用等也都对实验有影响。本综述包括了益生菌对CKD的潜在作用机制和研究方法的进展,对以后精准医疗模式下防治CKD提供新的思路和靶点。

关键词：益生菌慢性肾脏疾病肠道益生菌制剂

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鞘氨醇激酶在血管相关疾病中的保护作用及其机制研究进展

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心血管病学进展 2018年第1期39卷 87-89页

作者：徐倩哈小琴兰州军区兰州总医院甘肃兰州730050 兰州大学第二医院甘肃兰州730050

鞘氨醇激酶是调控机体鞘脂代谢的重要激酶之一,具有广泛的生物学作用。越来越多的研究表明,鞘氨醇激酶与临床多种血管疾病的发生发展密切相关。机体缺血或血管损伤时,鞘氨醇激酶可通过诱导血管新生、调节血管收缩以及改善血管内皮细胞... 详细信息

鞘氨醇激酶是调控机体鞘脂代谢的重要激酶之一,具有广泛的生物学作用。越来越多的研究表明,鞘氨醇激酶与临床多种血管疾病的发生发展密切相关。机体缺血或血管损伤时,鞘氨醇激酶可通过诱导血管新生、调节血管收缩以及改善血管内皮细胞应激耐受能力等多种方式避免血管内皮细胞的应激损伤,从而维持血管的完整性。鞘氨醇激酶对血管相关疾病的保护作用可通过鞘氨醇激酶/1-磷酸鞘氨醇-RAS/细胞外调节蛋白激酶及鞘氨醇激酶1/1-磷酸鞘氨醇/沉默信息调节因子1等多条信号通路实现。现根据已报道的文献,针对鞘氨醇激酶在血管相关疾病中的保护作用及其机制做一综述。

关键词：鞘氨醇激酶血管疾病保护机制

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PGLYRP1 protein as a novel mediator of cellular dialogue in neuroinflammation

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Neural Regeneration Research 2025年第7期20卷 1993-1994页

作者： Anup Bhusal Won-ha Lee Kyoungho Suk Department of Pharmacology School of MedicineKyungpook National UniversityDaeguRepublic of Korea BK21 Plus KNU Biomedical Convergence Program Department of Biomedical ScienceSchool of MedicineKyungpook National UniversityDaeguRepublic of Korea School of Life Sciences BK21 plus KNU Creative BioResearch GroupKyungpook National UniversityDaeguRepublic of Korea Brain Science and Engineering Institute Kyungpook National UniversityDaeguRepublic of Korea

Neuroinflammation has been identified as a crucial element in several neurological disorders. Glial cells play a critical role in directing neuroinflammation, both in deleterious and beneficial ways.

关键词： inflammation considerably

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