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A smoothed assumed enhanced strain method for frictional contact with constant strain elements

Journal of Rock Mechanics and Geotechnical Engineering 2024年第11期16卷 4369-4385页

作者： Fushen Liu Wei Sun Minghui Li xiaonan shang Computing Center for Geotechnical Engineering Zhejiang UniversityHangzhou310058China Research Center of Coastal and Urban Geotechnical Engineering Zhejiang UniversityHangzhou310058China Engineering Research Center of Urban Underground Development of Zhejiang Province Hangzhou310058China School of Civil Engineering Sun Yat-sen UniversityGuangzhou510275China School of Energy Resource China University of Geosciences(Beijing)Beijing100083China

This paper presents a finite element framework for imposing frictional contact conditions on embedded fracture faces,implemented by the constant-strain assumed enhanced strain(AES)method,where penalty method is used to impose both non-penetration constraint and Coulomb’s law of *** proposed constant-strain AES method for modeling embedded frictional contact can be cast into an integration algorithm similar to those used in the classical plasticity theory,where displacement jump is calculated from the local traction equilibrium at Gauss point,so the method does not introduce any additional global degrees of ***,constant-strain elements are often desirable in practice because they can be easily created automatically for large-scale engineering applications with complicated *** encountered in other enriched finite element methods for frictional contact,the problem of normal contact pressure oscillations is also observed in the constant-strain AES ***,we developed a strain-smoothing procedure to effectively mitigate the *** investigated and verified the proposed AES framework through several numerical examples,and illustrated the capability of this method in solving challenging nonlinear frictional contact problems.

关键词： Assumed enhanced strain(AES)method Frictional contact Strain-smoothing method Penalty method

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马铃薯X病毒荧光定量PCR检测体系的建立及应用

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植物保护 2016年第3期42卷 165-169页

作者：尚晓楠吴蓓蕾中国农业科学院植物保护研究所植物病虫害生物学国家重点实验室北京100193

马铃薯X病毒(Potato virus X,PVX)是危害茄科作物的一种重要病毒,为了建立特异性检测PVX的实时荧光定量PCR体系,本研究以PVX-1985分离物中外壳蛋白(coat protein,CP)基因序列为模板,设计引物构建重组质粒并选择扩增效率高、特异性强的... 详细信息

马铃薯X病毒(Potato virus X,PVX)是危害茄科作物的一种重要病毒,为了建立特异性检测PVX的实时荧光定量PCR体系,本研究以PVX-1985分离物中外壳蛋白(coat protein,CP)基因序列为模板,设计引物构建重组质粒并选择扩增效率高、特异性强的引物成功构建出标准曲线。利用建立的体系,成功检测到以含pCaPVX440侵染性克隆载体的农杆菌C58C1接种后的本氏烟(Nicotiana benthamiana)中PVX病毒RNA的拷贝数。

关键词：马铃薯X病毒荧光定量PCR 本氏烟

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盾构滚刀破岩的近场动力学模拟简述

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地下空间与工程学报 2023年第S1期19卷 87-96页

作者：朱建才尚肖楠刘福深袁逢逢浙江大学平衡建筑研究中心杭州310012 浙江大学建筑设计研究院有限公司杭州310012 浙江大学滨海和城市岩土工程研究中心杭州310058 浙江省城市地下空间开发工程技术研究中心杭州310058 浙江大学岩土工程计算中心杭州310058 中铁隧道股份有限公司郑州450001

盾构施工是目前修建隧道的主要方法之一。在盾构施工中,岩土材料在刀具作用下的响应是盾构掘进主要关心的内容之一。滚刀是盾构机破岩的主要刀具,从数值模拟的角度出发,首先总结了目前应用于滚刀破岩的数值方法,包括有限元法、离散元法... 详细信息

盾构施工是目前修建隧道的主要方法之一。在盾构施工中,岩土材料在刀具作用下的响应是盾构掘进主要关心的内容之一。滚刀是盾构机破岩的主要刀具,从数值模拟的角度出发,首先总结了目前应用于滚刀破岩的数值方法,包括有限元法、离散元法以及其他数值方法,并分析了以上方法的不足之处;为了突破现有方法在滚刀破岩领域遇到的瓶颈,提供了一种全新的思路—利用近场动力学模拟滚刀破岩,并简单阐述该方法的理论,同时列举了近场动力学在模拟岩石裂纹扩展以及破冰领域的成功案例;最后利用Peridigm模拟单滚刀破岩的算例,表明将近场动力学应用于模拟岩土材料在滚刀作用下损伤的演化及裂纹扩展等方面将有很大的应用前景。

关键词：滚刀破岩数值方法总结近场动力学 Peridigm

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油茶内生拮抗细菌的筛选、鉴定及防效

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中国生物防治学报 2021年第3期37卷 575-583页

作者：尚笑男刘君昂冯福山周国英中南林业科技大学长沙410004 人工林病虫害防控国家林业局重点实验室/森林有害生物防控湖南省重点实验室/经济林培育与保护教育部重点实验室长沙410004

为筛选出对油茶炭疽菌有拮抗效果的生防菌株,以油茶健康叶为对象,平板对峙法筛选,利用形态和生理生化特征以及16S rDNA基因和gyrA基因序列对菌株进行鉴定,测定其抑菌活性,并将该菌株与枯草芽胞杆菌Y13复配进行林间防效试验。结果表明,... 详细信息

为筛选出对油茶炭疽菌有拮抗效果的生防菌株,以油茶健康叶为对象,平板对峙法筛选,利用形态和生理生化特征以及16S rDNA基因和gyrA基因序列对菌株进行鉴定,测定其抑菌活性,并将该菌株与枯草芽胞杆菌Y13复配进行林间防效试验。结果表明,对油茶炭疽病菌拮抗作用效果较强的内生菌株为HBMC-B05;菌落为乳白色,不透明,边缘不整齐,中间有凸起;分子鉴定结果显示菌株HBMC-B05为贝莱斯芽胞杆菌Bacillus velezensis,其对果生炭疽菌Collectotrichum fructicola,抑菌率可达到81.31%,对另外3种油茶炭疽病病原菌抑菌率可达到70%以上,对其他4种致病菌也有抑制作用;复合菌剂对油茶病害的田间防治效果达到59%以上。该菌株的抑菌具有广谱性,可为油茶病害的生物防治扩充菌种资源,具有广泛的应用前景。

关键词：油茶内生细菌分离鉴定抑菌活性林间防效

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Immunity of Unloaded Dendritic Cells in Lung Melanoma of Mice

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Journal of Huazhong University of Science and Technology(Medical Sciences) 2007年第4期27卷 381-384页

作者：刘红菊辛建保陶晓南尚丹周琼 Department of Respiratory Diseases Union HospitalTongji Medical CollegeHuazhong University of Science and Technology

In order to investigate the immunity of unloaded dendritic cells （DCs） derived from murine bone marrow to preexisting lung melanoma metastases of mice, MO5 were intravenously injected to induce lung metastases in syngeneic C57BI-/6 mice. Unloaded GM-CSF DCs, PBS and DCs＋SIINFEKEL were subcutaneously injected into the mice, which were divided as experimental group, negative control group and positive control group respectively. Monoclonal antibody was used to deplete NK or T cells separately. The immunity-inhibitory effects on the lung melanoma were observed and the corresponding effector cells were examined. It was found that in the experimental and positive groups, the regression was induced in metastatic nodules in the lungs of tumor-bearing mice, but abrogated by treatment with anti-asialo-GM1 but not anti-CD8. It was concluded that the unloaded DCs could suppress the lung melanoma metastases to some extent, which was mediated by NK cells, and could be used as a potent therapeutic agents for lung tumor.

关键词： dendritic cell NK cell lung tumor immunity

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某品牌抗sp100抗体IgG检测试剂盒的性能评价

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医疗装备 2023年第12期36卷 13-17页

作者：尚晓楠尤瑞国李忠信谢茜郑州安图生物工程股份有限公司河南郑州450016

目的对郑州安图生物工程股份有限公司生产的抗sp100抗体Ig G检测试剂盒进行性能评价,为原发性胆汁性胆管炎(PBC)的辅助诊断提供可靠指标。方法参照美国临床和实验室标准协会(CLSI)的EP文件评价抗sp100抗体IgG检测试剂盒的灵敏度、精密... 详细信息

目的对郑州安图生物工程股份有限公司生产的抗sp100抗体Ig G检测试剂盒进行性能评价,为原发性胆汁性胆管炎(PBC)的辅助诊断提供可靠指标。方法参照美国临床和实验室标准协会(CLSI)的EP文件评价抗sp100抗体IgG检测试剂盒的灵敏度、精密度、线性区间、准确度、抗干扰等性能,同时与参比试剂盒进行临床比对。结果抗sp100抗体Ig G检测试剂盒的空白限(LoB)、检出限(LoD)、定量限(LoQ)分别为1.0、2.0、3.0 AU/ml,重复性的变异系数(CV)<4.00%,中间精密度的CV<6.00%,线性区间为10~200 AU/ml,准确度符合要求[回收率R在90%~110%之间];标本中的血红蛋白、三酰甘油、胆红素、生物素水平分别为3000 mg/dl、6000 mg/dl、50 mg/dl、250 ng/ml时,干扰率在±10%范围内。抗sp100抗体IgG检测试剂盒与两个参比试剂盒检测结果的总符合率分别为98.40%和99.20%,一致性均较好(Kappa=0.950、0.975,P<0.001)。结论该试剂盒性能良好,可满足临床检测抗sp100抗体IgG水平的需要。

关键词：磁微粒抗sp100抗体IgG 化学发光性能评估

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新型高温井井下控制阀的研制与应用

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石油机械 2005年第7期33卷 41-42页

作者：胡义李啸南尚跃强逯国成黄侠崔晓霖胜利油田有限公司孤岛采油厂胜利油田有限公司采油工艺研究院胜利油田有限公司东辛采油厂

针对稠油注汽热采井中,压井液、洗井液进入地层使井下温度降低,造成转抽后开井困难;转抽前井内压力释放不彻底,易发生井喷或外溢等情况,研制了新型高温井井下控制阀。该阀的创新点:(1)采用齿轮齿条传动机构把管柱的直线运动变为阀球旋... 详细信息

针对稠油注汽热采井中,压井液、洗井液进入地层使井下温度降低,造成转抽后开井困难;转抽前井内压力释放不彻底,易发生井喷或外溢等情况,研制了新型高温井井下控制阀。该阀的创新点:(1)采用齿轮齿条传动机构把管柱的直线运动变为阀球旋转运动,使阀球的开关更灵活,由锁爪、弹簧和支撑环组成的锁紧机构牢靠,不受阀球上下压差的影响;(2)球面金属密封在高温高压下密封性好;(3)阀球内通径大,打开后阀球通道与管柱同心,成为流体流动的畅通通道;(4)提放管柱开关球阀操作方便,可用于注汽热采井、直井、非热采井和大斜度井。

关键词：控制阀高温井井下研制应用热采井温度降低压力释放旋转运动直线运动传动机构齿轮齿条锁紧机构金属密封流体流动操作方便大斜度井阀球压井液洗井液管柱支撑环密封性注汽开关通道稠油井喷压差

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Nonconserved epitopes dominate reverse preexisting T cell immunity in COVID-19 convalescents

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Signal Transduction and Targeted Therapy 2024年第7期9卷 2984-2998页

作者： Xin Wang Jie Zhang Maoshun Liu Yuanyuan Guo Peipei Guo xiaonan Yang Bingli shang Min Li Jinmin Tian Ting Zhang Xi Wang Ronghua Jin Jikun Zhou George F.Gao Jun Liu Department of Epidemiology School of Public HealthCheeloo College of MedicineShandong UniversityJinan 250012China NHC Key Laboratory of Biosafety Research Unit of Adaptive Evolution and Control of Emerging VirusesChinese Academy of Medical SciencesNational Institute for Viral Disease Control and PreventionChinese Center for Disease Control and Prevention(China CDC)Beijing 102206China Beijing Key Laboratory of Emerging Infectious Diseases Institute of Infectious DiseasesBeijing Ditan HospitalCapital Medical UniversityBeijing 100015China Beijing Institute of Infectious Diseases Beijing 100015China National Center for Infectious Diseases Beijing Ditan HospitalCapital Medical UniversityBeijing 100015P.R.China School of Laboratory Medicine and Life Sciences Wenzhou Medical UniversityWenzhou 325035China School of Ophthalmology&Optometry Wenzhou Medical UniversityWenzhou 325027China Collaborative Innovation Centre of Regenerative Medicine and Medical BioResource Development and Application Co-constructed by the Province and Ministry Guangxi Medical UniversityNanningGuangxi 530021China Shijiazhuang Fifth Hospital Shijiazhuang 050011China CAS Key Laboratory of Pathogen Microbiology and Immunology Institute of MicrobiologyChinese Academy of Sciences(CAS)Beijing 100101China Savaid Medical School University of Chinese Academy of SciencesBeijing 100049China

The herd immunity against SARS-CoV-2 is continuously consolidated across the world during the ongoing ***,the potential function of the nonconserved epitopes in the reverse preexisting cross-reactivity induced by SARS-CoV-2 to other human coronaviruses is not well *** our research,we assessed T cell responses to both conserved and nonconserved peptides shared by SARS-CoV-2 and SARS-CoV,identifying cross-reactive CD8^(+)T cell epitopes using enzyme-linked immunospot and intracellular cytokine staining ***,in vitro refolding and circular dichroism were performed to evaluate the thermal stability of the HLA/peptide ***,single-cell T cell receptor reservoir was analyzed based on tetramer ***,we discovered that cross-reactive T cells targeting SARS-CoV were present in individuals who had recovered from COVID-19,and identified SARS-CoV-2 CD8^(+)T cell epitopes spanning the major structural antigens.T cell responses induced by the nonconserved peptides between SARS-CoV-2 and SARS-CoV were higher and played a dominant role in the cross-reactivity in COVID-19 ***-T cell reactivity was also observed within the identified series of CD8^(+)T cell *** representative immunodominant peptide pairs,although the HLA binding capacities for peptides from SARS-CoV-2 and SARS-CoV were similar,the TCR repertoires recognizing these peptides were *** results could provide beneficial information for the development of peptide-based universal vaccines against coronaviruses.

关键词： immunity conserved assessed

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Optimizing a high-sensitivity NanoLuc-based bioluminescence system for in vivo evaluation of antimicrobial treatment

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mLife 2023年第4期2卷 462-478页

作者： Weilong shang Zhen Hu Mengyang Li Yuting Wang Yifan Rao Li Tan Juan Chen xiaonan Huang Lu Liu He Liu Zuwen Guo Huagang Peng Yi Yang Qiwen Hu Shu Li Xiaomei Hu Jiao Zou Xiancai Rao Department of Microbiology College of Basic Medical SciencesKey Laboratory of Microbial Engineering Under the Educational Committee in ChongqingArmy Medical University(Third Military Medical University)ChongqingChina Department of Microbiology School of MedicineChongqing UniversityChongqingChina Department of Emergency Medicine Xinqiao HospitalArmy Medical University(Third Military Medical University)ChongqingChina Department of Pharmacy Xinqiao HospitalArmy Medical University(Third Military Medical University)ChongqingChina Department of Military Cognitive Psychology School of PsychologyArmy Medical University(Third Military Medical University)ChongqingChina.

Focal and systemic infections are serious threats to human *** models enable the development of new drugs and therapeutic *** vivo,animal bioluminescence(BL)imaging has been used with bacterial reporter strains to evaluate antimicrobial treatment ***,high-sensitivity bioluminescent systems are required because of the limited tissue penetration and low brightness of the BL signals of existing ***,we report that NanoLuc(Nluc)showed better performance than LuxCDABE in ***,the retention rate of plasmid constructs in bacteria was *** construct stable Staphylococcus aureus reporter strains,a partner protein enolase(Eno)was identified by screening of *** strain USA300 for fusion expression of Nluc-based luciferases,including Nluc,Teluc,and *** substrates,such as hydrofurimazine(HFZ),furimazine(FUR),and diphenylterazine(DTZ),were used to optimize a stable reporter strain/substrate pair for BL *** USA300/Eno-Antares2/HFZ produced the highest number of photons of orange-red light in vitro and enabled sensitive BL tracking of *** in vivo,with sensitivities of approximately 10 CFU from mouse skin and 750 CFU from mouse ***300/Eno-Antares2/HFZ was a powerful combination based on the longitudinal evaluation of the therapeutic efficacy of *** optimized *** Eno-Antares2/HFZ pair provides a technological advancement for the in vivo evaluation of antimicrobial treatment.

关键词： antimicrobial evaluation bioluminescence imaging Nluc-based luciferases Staphylococcus aureus therapeutic efficacy

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Constructing a high-density linkage map for Gossypium hirsutum × Gossypium barbadense and identifying QTLs for lint percentage

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Journal of Integrative Plant Biology 2015年第5期57卷 450-467页

作者： Yuzhen Shi Wentan Li Aiguo Li Ruihua Ge Baocai Zhang Junzhi Li Guangping Liu Junwen Li Aiying Liu Haihong shang Juwu Gong Wankui Gong Zemao Yang Feiyü Tang Zhi Liu Weiping Zhu Jianxiong Jiang xiaonan Yu Tao Wang Wei Wang Tingting Chen Kunbo Wang Zhengsheng Zhang Youlu Yuan State Key Laboratory of Cotton Biology Key Laboratory of Biological and Genetic Breeding of Cottonthe Ministry of AgricultureInstitute of Cotton Researchthe Chinese Academy of Agricultural Sciences Agricultural College of Jiangxi Agricultural University Academy of Agricultural Sciences of Luohe City of Henan Province College of Bioscience and Biotechnology Hunan Agricultural University College of Agronomy Hunan Agricultural University Agricultural College of Southwest University

To introgress the good fiber quality and yield from Gossypium barbadense into a commercial Upland cotton variety, a high‐density simple sequence repeat （SSR） genetic linkage map was developed from a BC1F1 population of Gossypium hirsutum × Gossypium barbadense. The map com-prised 2,292 loci and covered 5115.16 centiMorgan （cM） of the cotton AD genome, with an average marker interval of 2.23 cM. Of the marker order for 1,577 common loci on this new map, 90.36% agrees well with the marker order on the D genome sequence genetic map. Compared with five pub-lished high‐density SSR genetic maps, 53.14% of marker loci were newly discovered in this map. Twenty‐six quantitative trait loci （QTLs） for lint percentage （LP） were identified on nine chromosomes. Nine stable or common QTLs could be used for marker‐assisted selection. Fifty percent of the QTLs were from G. barbadense and increased LP by 1.07%–2.41%. These results indicated that the map could be used for screening chromosome substitution segments from G. barbadense in the Upland cotton background, identifying QTLs or genes from G. barbadense, and further developing the gene pyramiding effect for improving fiber yield and quality.

关键词： Cotton (Gossypium spp.) genetic linkage map Gossypiumbarbadense lint percentage simple sequence repeat

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