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Targeting GATA1 and p2x7r Locus Binding in Spinal Astrocytes Suppresses Chronic Visceral Pain by Promoting DNA Demethylation

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Neuroscience Bulletin 2022年第4期38卷 359-372页

作者： Yan-Yan Wu Hai-Long Zhang xiaomin lu Han Du Yong-Chang Li Ping-An Zhang Guang-Yin Xu Jiangsu Key Laboratory of Neuropsychiatric Diseases Institute of NeuroscienceSoochow UniversitySuzhou 215123China Department of Oncology The Affiliated Haian Hospital of Nantong UniversityNantong 226600China Center for Translational Medicine The Affiliated Zhangjiagang Hospital of Soochow UniversitySuzhou 215600China

Irritable bowel syndrome is a gastrointestinal disorder of unknown etiology characterized by widespread,chronic abdominal pain associated with altered bowel movements.Increasing amounts of evidence indicate that injury and inflammation during the neonatal period have long-term effects on tissue structure and function in the adult that may predispose to gastrointestinal diseases.In this study we aimed to investigate how the epigenetic regulation of DNA demethylation of the p2x7r locus guided by the transcription factor GATA binding protein 1(GATA1)in spinal astrocytes affects chronic visceral pain in adult rats with neonatal colonic inflammation(NCI).The spinal GATA1 targeting to DNA demethylation of p2x7r locus in these rats was assessed by assessing GATA1 function with luciferase assay,chromatin immunoprecipitation,patch clamp,and interference in vitro and in vivo.In addition,a decoy oligodeoxynucleotide was designed and applied to determine the influence of GATA1 on the DNA methylation of a p2x7r CpG island.We showed that NCI caused the induction of GATA1,Ten-eleven translocation 3(TET3),and purinergic receptors(P2X7Rs)in astrocytes of the spinal dorsal horn,and demonstrated that inhibiting these molecules markedly increased the pain threshold,inhibited the activation of astrocytes,and decreased the spinal sEPSC frequency.NCI also markedly demethylated the p2x7r locus in a manner dependent on the enhancement of both a GATA1–TET3 physical interaction and GATA1 binding at the p2x7r promoter.Importantly,we showed that demethylation of the p2x7r locus(and the attendant increase in P2X7R expression)was reversed upon knockdown of GATA1 or TET3 expression,and demonstrated that a decoy oligodeoxynucleotide that selectively blocked the GATA1 binding site increased the methylation of a CpG island in the p2x7r promoter.These results demonstrate that chronic visceral pain is mediated synergistically by GATA1 and TET3 via a DNA-demethylation mechanism that controls p2x7r transcription in spinal dorsal horn astrocytes,and pr

关键词： Chronic visceral pain GATA binding protein 1 Ten-eleven translocation 3 Purinergic receptor Epigenetic regulation Spinal astrocytes

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Drainage pattern recognition method considering local basin shape based on graph neural network

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International Journal of Digital Earth 2023年第1期16卷 593-619页

作者： Wenning Wang Haowen Yan xiaomin lu Yi He Tao Liu Wende Li Pengbo Li Fang Xu Faculty of Geomatics Lanzhou Jiaotong UniversityLanzhouPeople’s Republic of China Lanzhou Ji Er Ai Si Spatial Information Technology Company Limited LanzhouPeople’s Republic of China National-Local Joint Engineering Research Center of Technologies and Applications for National Geographic State Monitoring LanzhouPeople’s Republic of China Gansu Provincial Engineering Laboratory for National Geographic State Monitoring LanzhouPeople’s Republic of China People’s Republic of China Navigation Publication Press TianjinPeople’s Republic of China

Drainage pattern recognition is crucial for geospatial understanding and hydrologic modelling.Currently,drainage pattern recognition methods employ geometric measures of overall and local features of river networks but lack measures of river basin unit shape features,so that potential correlations between river segments are usually ignored,resulting in poor drainage pattern recognition results.In order to overcome this problem,this paper proposes a supervised graph neural network method that considers the local basin unit shape of river networks.First,based on the overall hierarchy of the river networks,the confluence angle of river segments and the shape of river basin units,multiple drainage pattern classification features are extracted.Then,typical drainage pattern samples from the multi-scale NSDI and USGS databases are used to complete the training,validation and testing steps.Experimental results show that the drainage pattern indexes proposed can describe the characteristics of different drainage patterns.The method can effectively sample the adjacent river segments,flexibly transfer the associated pattern features among river segment neighbours,and aggregate the deeper characteristics of the river networks,thus improving the drainage pattern recognition accuracy relative to other methods and reliably distinguishing different drainage patterns.

关键词： River drainage pattern recognition Basin unit shape supervised learning graph neural networks

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顾及权重信息的地图点群目标自动综合方法

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测绘学报 2024年第2期53卷 398-398页

作者：禄小敏兰州交通大学测绘与地理信息学院甘肃兰州730070 地理国情监测技术应用国家地方联合工程研究中心甘肃兰州730070

在小比例尺地图上,许多地物呈点群状分布。当地图比例尺进一步缩小时,点状符号之间会出现相互堆叠、覆盖等情形。为了保持地图的清晰度、层次特征与美观性,需要对点群进行综合,即从原始点群中抽取出一定数量相对重要的点而删除相对次要... 详细信息

在小比例尺地图上,许多地物呈点群状分布。当地图比例尺进一步缩小时,点状符号之间会出现相互堆叠、覆盖等情形。为了保持地图的清晰度、层次特征与美观性,需要对点群进行综合,即从原始点群中抽取出一定数量相对重要的点而删除相对次要点。点的权重反映了单个点在点群整体中的重要性程度,在点群综合算法设计中非常关键。按照点群综合过程中对权重信息的顾及情况,可以将已有的点群综合算法分为两大类:第1类算法在点群综合过程中没有顾及其权重信息,第2类算法在综合过程中虽然顾及到了权重信息,但仍然存在点群权重设定比较主观、综合结果缺乏现势参考价值,以及没有顾及道路网在点群综合中的制约与影响作用等问题。

关键词：地图比例尺小比例尺点群综合点状符号自动综合权重信息点群目标算法设计

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DP算法自动实现方法:以河流化简为例

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武汉大学学报（信息科学版） 2024年第2期49卷 264-270页

作者：闫浩文张鑫港禄小敏李蓬勃兰州交通大学测绘与地理信息学院甘肃兰州730070 地理国情监测技术应用国家地方联合工程研究中心甘肃兰州730070 甘肃省地理国情监测工程实验室甘肃兰州730070

曲线化简是自动地图综合的重要内容,但其中广泛应用的DP(Douglas-Peucker)算法却是非自动化的,原因是需要在算法执行之初由人工输入距离阈值ε。为此,首先提出了一个多尺度曲线相似度的计算公式;然后基于该公式,以河流数据为例给出了地... 详细信息

曲线化简是自动地图综合的重要内容,但其中广泛应用的DP(Douglas-Peucker)算法却是非自动化的,原因是需要在算法执行之初由人工输入距离阈值ε。为此,首先提出了一个多尺度曲线相似度的计算公式;然后基于该公式,以河流数据为例给出了地图比例尺与曲线目标相似度的函数关系推导方法和曲线目标相似度与ε的函数关系推导方法,进而得出了ε与比例尺的函数关系;最后实现了DP算法的自动化。实验研究表明,利用提出的自动化DP算法可以获得指定地理区域不同比例尺的水系要素的化简结果,化简结果与经验丰富的制图员的手工化简结果的相似度平均值为0.927,相似度总体表现良好,表明了该方法良好的可靠性和较高的智能化。

关键词：曲线化简地图综合 Douglas-Peucker算法相似度河流数据

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Identification and isolation of Mu-flanking fragments from maize

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Journal of Genetics and Genomics 2008年第4期35卷 207-213页

作者： Yijun Wang Guangming Yin Qin Yang Jihua Tang xiaomin lu Schuyler S. Korban Mmghang Xu Agricultural College Yangzhou University Yangzhou 225009 China National Maize Improvement Center of China China Agricultural University Beijing 100094 China Corn Research Institute Henan Agricultural University Zhengzhou 450002 China Department of Natural Resources and Environmental Sciences University of Illinois Urbana 1L61801 USA

Transposable elements have been utilized as mutagens to create mutant libraries for functional genomics. Isolation of genomic segments flanking the insertion Mutator （Mu） is a key step in insertion mutagenesis studies. Herein, we adopted a modified AFLP method to identify and isolate Mu-flanking fragments from maize. The method consists of the following steps： 1） double-digestion of genomic DNA with Bgl II/Msp I and ligation of digested fragments to the Bgl II- and Msp I-adaptors; 2） enrichment of a subset of Bgl II/Msp I fragments followed by selective amplification of the Mu-flanking fragments; 3） simultaneous display of AFLP bands derived from the flanking regions for both insert and native Mu transposons; 4） identification and isolation of AFLP bands resulting from Mu insertions by comparing the banding profiles between Mu-induced mutants and their parental lines; and 5） confirmation of flanking fragments related to these Mu insertions. Using this approach, we have isolated flanking fragment（s） resulting from Mu insertion for every Mu-induced mutant, and one such fragment, M196-FF, is found to contain a partial sequence of the DNA topoisomerase I gene Topl. Moreover, the modified AFLP method including all restriction enzymes, adaptors and primers has been optimized in this study. The modified AFLP method has been proved to be simple and efficient in the isolation of Mu-flanking fragments and will find its usefulness in the functional genomics of maize.

关键词： Mutator transposon flanking fragment AFLP maize

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Identification and Fine Mapping of rhm1 Locus for Resistance to Southern Corn Leaf Blight in Maize

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Journal of Integrative Plant Biology 2012年第5期54卷 321-329页

作者： Yuanzeng Zhao xiaomin lu Chaoxian Liu Haiying Guan Mei Zhang Zhongfeng Li Hongwei Cai Jinsheng Lai State Key Laboratory of Agrobiotechnology and National Maize Improvement Center Department of Plant Genetics and BreedingChina Agricultural UniversityBeijing 100193China Department of Life Science and Technology Henan Institute of Science and TechnologyXinxiang 453003China Henan Academy of Agricultural Sciences Zhengzhou 450002China

rhml is a major recessive disease resistance locus for Southern corn leaf blight （SCLB）. To further narrow down its genetic position, F2 population and BCIFI population derived from the cross between resistant （H95rhm） and susceptible parents （H95） of maize （Zea mays） were constructed. Using newly developed markers, rhml was initially delimited within an interval of 2.5 Mb, and then finally mapped to a 8.56 kb interval between InDel marker IDP961-503 and simple sequence repeat （SSR） marker A194149--1. Three polymorphic markers IDP961-504, IDP B2-3 and A194149-2 were shown to be co-segregated with the rhml locus. Sequence analysis of the 8.56 kb DNA fragment revealed that it contained only one putative gene with a predicted amino acid sequence identical to lysine histidine transporter 1 （LHT1）. Comparative sequence analysis indicated that the LHT1 in H95rhrn harbors a 354 bp insertion in its third exon as compared with that of susceptible alleles in B73, H95 and Mo17. The 354 bp insertion resulted in a truncation of the predicted protein of candidate resistance allele （LHT1-H95rhm）. Our results strongly suggest LHTI as the candidate gene for rhml against SCLB. The tightly linked molecular markers developed in this study can be directly used for molecular breeding of resistance to Southern corn leaf blight in maize.

关键词： Bipolaris maydis fine-mapping maize rhml Southern corn leaf blight Zea mays.

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Maize WI5 encodes an endo-1,4-β-xylanase required for secondary cell wall synthesis and water transport in xylem

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Journal of Integrative Plant Biology 2020年第10期62卷 1607-1624页

作者： Xiaojiao Hu Yang Cui xiaomin lu Weibin Song Lei Lei Jinjie Zhu Jinsheng Lai Lizhu E Haiming Zhao State Key Laboratory of Plant Physiology and Biochemistry National Maize Improvement CenterCollege of Agronomy and BiotechnologyChina Agricultural UniversityBeijing 100193China

Water transport from roots to leaves through xylem is important for plant growth and development.Defects in water transport can cause drought stress,even when there is adequate water in the soil.Here,we identi-fied the maize(Zea mays)wilty5(wi5)mutant,which ex-hibits marked dwarfing and leaf wilting throughout most of its life cycle under normal growth conditions.wilty5 seed-lings exhibited lower xylem conductivity and wilted more rapidly under drought,NaCl,and high temperature treat-ments than wild-type plants.Map-based cloning revealed that WI5 encodes an active endo-1,4-β-xylanase from gly-cosyl dehydration family 10,which mainly functions in degrading and reorganizing cell wall xylan.Reverse-transcription polymerase chain reaction andβ-glucuronidase assays revealed that WI5 is highly expressed in stems,especially in internodes undergoing secondary wall as-sembly.RNA sequencing suggested that WI5 plays a unique role in internode growth.Immunohistochemistry and electron microscopy confirmed that wi5 is defective in xylan deposition and secondary cell wall thickening.Lignin deposition and xylan content were markedly re-duced in wi5 compared to the wild-type plants.Our results suggest that WI5 functions in xylem cell wall thickening through its xylanase activity and thereby regulates xylem water transport,the drought stress response,and plant growth in maize.

关键词： dwarf fir transport

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Nonlinear temporal pulse cleaning techniques and application

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High Power Laser Science and Engineering 2013年第2期1卷 98-101页

作者： Yi Xu Jianzhou Wang Yansui Huang Yanyan Li xiaomin lu Yuxin Leng State Key Laboratory of High Field Laser Physics Shanghai Institute of Optics and Fine MechanicsChinese Academy of Sciences

Two different pulse cleaning techniques for ultra-high contrast laser systems are comparably analysed in this work.The first pulse cleaning technique is based on noncollinear femtosecond optical-parametric amplification(NOPA)and second-harmonic generation(SHG)processes.The other is based on cross-polarized wave(XPW)generation.With a double chirped pulse amplifier(double-CPA)scheme,although temporal contrast enhancement in a high-intensity femtosecond Ti:sapphire chirped pulse amplification(CPA)laser system can be achieved based on both of the techniques,the two different pulse cleaning techniques still have their own advantages and are suitable for different contrast enhancement requirements of different laser systems.

关键词： pulse cleaning temporal contrast chirped pulse amplification

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Molecular mapping and candidate gene analysis of the semi-dominant gene Vestigial glume1 in maize

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The Crop Journal 2019年第5期7卷 667-676页

作者： Chaoxian Liu Yuanzeng Zhao Yang Bai xiaomin lu Weibin Song Liping Qin Yilin Cai Maize Research Institute Southwest UniversityChongqing 400715China Henan Institute of Science and Technology Xinxiang 453003HenanChina Henan Academy of Agricultural Sciences Zhengzhou 450002HenanChina National Maize Improvement Center of China China Agricultural UniversityBeijing 100193China

The glume is an organ of the maize spikelet and plays important roles in anther and kernel development.Vestigial glume1(Vg1)is a classic mutant associated with ligule and glume development.Here we report the phenotypic characterization,fine mapping,and candidate gene analysis of the Vg1 mutant.Vg1 is a semi-dominant and pleiotropic gene,and also affects plant height,ear height,and tassel length.Vg1 ligule degeneration begins at the first leaf,and the Vg1 tassel and ear can be distinguished from those of wild-type plants when their lengths reach respectively 55 mm and 51 mm.Using a BC3 mapping population of 11,445 plants,we delimited the Vg1 functional site to an interval of 7.4 kb,flanked by the markers InDelLM and CRM6.A putative cyclopropane fatty-acid synthase gene(ZmCPA-FAS1)was hypothesized to underlie the mutant phenotype.We detected a Helitron insertion in the sixth intron of ZmCPAFAS1.Its presence caused abnormal alternative splicing of ZmCPA-FAS1 that conferred new characteristics on the Vg1 mutant.These findings are a basis for further discovery of the molecularmechanism underlying glume development and a potential guide formaize breeding of small-glume varieties,especially sweet corn breeding.

关键词： Vestigial glume1 Fine mapping Candidate gene analysis ZmCPA-FAS1 Glume development

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Effect of a novel dietary supplement on pH levels of healthy volunteers:a pilot study

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Journal of Integrative Medicine 2013年第6期11卷 384-388页

作者： Stephen Douglas Anton xiaomin lu Ginny Bank Kacey Heekin Debbie Saha Pamela J.Dubyak Heather Ann Hausenblas Department of Aging and Geriatric Research University of Florida Department of Clinical and Health Psychology University of Florida Department of Biostatistics University of Florida RFI Ingredients Department of Sport and Exercise Sciences University of Florida Department of Sport and Exercise Sciences Jacksonville University

OBJECTIVE： To examine the effects of a greens alkalizing dietary supplement on urinary pH levels in individuals with lower-than-average pH levels. METHODS： The present study investigated the effects of an alkalizing formula （Reserveage Wholeganic GreensTM） on four individuals who had average urinary pH levels below 6.0 for three consecutive days. Following the three-day, baseline period, participants received Reserveage Wholeganic GreensTM for four consecutive days and were instructed to continue to measure their urine pH levels. Paired samples t-tests were used to examine pH levels before and after a four-day treatment period with Reserveage Wholeganic GreensTM. RESULTS： Compared to baseline, mean urine pH levels in all volunteers were significantly higher following the supplementation with Reserveage Wholeganic GreensTM （5.89 ±0.20 vs 5.56 ± 0.23; P〈0.01）. Participants＇ pH levels were also significantly higher than baseline on days 5, 6, and 7 of the treatment period （P 〈 0.05）. Noteworthy, on day 7, participants＇ mean pH levels were significantly higher than at the beginning of the treatment period （6.03 ± 0.15 at day 7 vs 5.65 ± 0.24 at day 4; P 〈 0.01）. CONCluSION： The findings of this study suggest that supplementation with Reserveage Wholeganic GreensTM has an alkalizing effect on the body and can increase the urine pH levels in individuals with lower-than-average pH levels.

关键词： dietary supplements urinalysis acidosis pilot study

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