检索结果-南通市图书馆

Amplifying protection against acute lung injury: Targeting both inflammasome and cGAS-STING pathway by Lonicerae Japonicae Flos-Forsythiae Fructus drug pair

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chinese Herbal Medicines 2024年第3期16卷 422-434页

作者： Junjie Li Ming Dong Qing yao Xu Dong Yuanyuan Chen Jincai Wen Yingjie Xu Zhixin Wu xiaomei Zhao Ye Xiu Xiaoyan Zhan Zhaofang Bai Xiaohe Xiao Chengde Medical University Department of Hepatology The Fifth Medical Center of PLA General Hospital China Military Institute of Chinese Materia Fifth Medical Center of Chinese PLA General Hospital National Key Laboratory of Kidney Diseases The Fifth Medical Center of PLA General Hospital School of Stomatology Heilongjiang Key Lab of Oral Biomedicine Materials and Clinical Application & Experimental Center for Stomatology EngineeringJiamusi University Southern Medical University

Objective:Acute lung injury(ALI) is characterized by inflammation and currently lacks an efficacious pharmacological intervention.The medicine combination of Lonicerae Japonicae Flos(LJF) and Forsythiae Fructus(FF) demonstrates combined properties in its anti-infective,anti-inflammatory,and therapeutic effects,particularly in alleviating respiratory symptoms.In previous studies,Chinese medicine has shown promising efficacy in lipopolysaccharides(LPS)-induced ALI.However,there have been no reports of LJF and FF pairing for lung injury.The aim of this study is to compare the efficacy of herb pair Lonicerae Japonicae Flos-Forsythiae Fructus(LF) with LJF or FF alone in the treatment of ALI,and to explore whether LJF and FF have a combined effect in the treatment of lung injury,along with the underlying mechanism involved.Methods:A total of 36 mice were divided into six groups(control,model,LJF,FF,LF,dexamethasone)based on the treatments they received after undergoing sham-operation/LPS tracheal instillation.H&E staining and pulmonary edema indexes were used to evaluate lung injury severity.Alveolar exudate cells(AECs) were counted based on cell count in bronchoalveolar lavage fluid(BALF),and neutrophil percentage in BALF was measured using flow cytometry.Myeloperoxidase(MPO) activity in BALF was measured using enzyme-linked immunosorbent assay(ELISA),while the production of IL-1β,TNF-α,and IL-6 in the lung and secretion level of them in BALF were detected by quantitative polymerase chain reaction(qPCR)and ELISA.The effect of LJF,FF,and LF on the expression of Caspase-1 and IL-1β proteins in bone marrow derived macrophages(BMDMs) supernatant was assessed using Western blot method under various inflammasome activation conditions.In addition,the concentration of IL-1β and changes in lactatedehydrogenase(LDH) release levels in BMDMs supernatant after LJF,FF,and LF administration,respectively,were measured using ELISA.Furthermore,the effects of LJF,FF and LF on STING and IRF3 phosphorylation in BMDMs were detected by West

关键词： acute lung injury BMDMs inflammasomes Lonicerae Japonicae Flos - Forsythia Fructus drug pair STING

Some thoughts on conducting and implementing clinical practice guidelines in a pandemic

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Chinese Medical Journal 2021年第8期134卷 910-912页

作者： xiaomei yao Ying-Hui Jin Benjamin Djulbegovic Departm ent of Health Research Evidence and ImpactMcMaster UniversityHamiltonOntarioCanada Center for Evidence-based and Translational Medicine Zhongnan Hospital of Wuhan UniversityWuhanHubei 430071China Department of Supportive Care Medicine Department of HematologyCity of Hope National Medical CenterDuarteCAUSAProgram for Evidence-based Medicine and Comparative Effectiveness ResearchDuarteCAUSA.

A pandemic is a disease that spreads worldwide.When disease is new such as coronavirus disease 2019(COVID-19),evidentiary basis,by definition,to prevent,diagnose,and treat the disease is limited.Nevertheless,clinical practice guidelines are urgently required to assist policy and healthcare workers to make informed decisions.But,acting in the high-quality evidentiary vacuum,that is,basing guidelines on poor-quality research can be counterproductive and potentially harmful.[1,2] Thus,guideline developers face a number of challenges during a pandemic like COVID-19 that we wish to address in this paper.

关键词： clinical evident conducting

在线全文

学校读者我要写书评

暂无评论

冰藏条件下不同贮存形式养殖大菱鲆品质对比

食品科学 2024年第12期45卷 243-252页

作者：庄小妹郑尧马园郭全友中国水产科学研究院东海水产研究所上海200090 上海海洋大学食品学院上海201306

为探究不同贮存形式对养殖大菱鲆(Scophthalmus maximus)品质的影响,测定其3种贮存形式(整鱼、去内脏和鱼片)在冰藏期间菌落总数、总挥发性盐基氮(total volatile base nitrogen,TVB-N)、硫代巴比妥酸(thiobarbituric acid,TBA)值、K值... 详细信息

为探究不同贮存形式对养殖大菱鲆(Scophthalmus maximus)品质的影响,测定其3种贮存形式(整鱼、去内脏和鱼片)在冰藏期间菌落总数、总挥发性盐基氮(total volatile base nitrogen,TVB-N)、硫代巴比妥酸(thiobarbituric acid,TBA)值、K值、水分分布、微观结构及蛋白降解程度的变化,采用电子舌采集其滋味轮廓,通过气相色谱-离子迁移谱对不同处理大菱鲆挥发性物质进行分析检测。结果表明:鱼片组在贮藏过程中菌落总数、TVB-N及TBA值较高,其中菌落总数在冰藏12 d时达7.05(lg(CFU/g)),超过贮藏终点。除此之外,鱼片组盐溶性蛋白质量分数在第19天时降至最低,为1.57%,同时肌纤维断裂和间隙增大的程度也更严重。与整鱼组相比,去内脏组第19天时菌落总数和TVB-N值均较高,分别为6.58(lg(CFU/g))和16.72 mg/100 g。贮藏末期整鱼组和去内脏组自由水含量占比分别为0.21%和0.28%,显著低于鱼片组(P<0.05),说明其肌肉组织结构也维持较好。最后根据气相色谱-离子迁移谱采集的指纹图谱,结合正交偏最小二乘判别分析及聚类热图分析可将不同冰藏时间下3种贮存形式大菱鲆区分开。综上所述,冰藏条件下鱼片组品质劣化速度最快,去内脏组次之,整鱼组最慢,研究结果可为替代大菱鲆传统“活品”流通方式提供参考。

关键词：大菱鲆冰藏整鱼去内脏鱼鱼片品质

Au-catalysed free-standing wurtzite structured InAs nanosheets grown by molecular beam epitaxy

在线全文

学校读者我要写书评

暂无评论

Nano Research 2019年第11期12卷 2718-2722页

作者： Qiang Sun Han Gao xiaomei yao Kun Zheng Pingping Chen Wei Lu Jin Zou Materias Engineering The University of Queensland St LuciaQLD 4072Australia State Key Laboratory for Infrared Physics Shanghai Institute of Techrical PlhysicsChinese Acadenry of Sciences500 Yutian RoadShanghai 200083China University of Chinese Academy of Sciences No.19A Yuquan RoadBeijing 100049China Institute of Microstructure and Properties of Advanced Materials Beijing University of TechnologyBeijing 100124China Centre for Microscopy and Microanalysis The University of QueenslandSt LuciaQLD 4072 Alustraia

In this study,we report the growth of free-standing InAs nanosheets using Au catalysts in molecular beam epitaxy.Detailed structural characterizations suggest that wurtzite structured InAs nanosheets,with features of extensive{1120}surfaces,grown along thedirection and adopted{0001}nanosheet/catalyst interfaces,are initiated from wurtzite structured[0001]nanowires as the inclined epitaxial growth due to relatively higher In concentrations in Au catalysts,and grown from these inclined nanostructures through catalyst-induced axial growth and their enhanced lateral growth under the high growth temperature.Based on the facts that the nanosheets contain large low energy{1120}surfaces and{0001}nanosheet/catalyst interfaces,the growth of our nanosheets is a thermodynamically driven process.This study provides new insights into fabricating free-standing Ⅲ-Ⅴ nanosheets for their applications in future nanoscale devices.

关键词： InAs nanosheet wurtzite structure catalyst supersaturation molecular beam epitaxy

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

玛曲县退牧还草工程实施效果评估

中国草地学报 2024年第3期46卷 47-59页

作者：张琴孙小妹蒲强胜姚宝辉张倩苏军虎甘肃农业大学资源与环境学院甘肃兰州730070 甘肃农业大学‒新西兰梅西大学草地生物多样性研究中心甘肃兰州730070 甘肃农业大学草业学院/草业生态系统教育部重点实验室/甘肃省草业科学工程实验室/中‒美草地畜牧业可持续发展研究中心甘肃兰州730070

为了综合评价甘肃省玛曲县在第二轮退牧还草工程(2005~2020年)实施中取得的成效,本文构建经济效益、生态效益、社会效益、工程的可持续性、工程的自身特性5个准则层、17个指标层的综合评价体系。采取随机抽样的方法展开问卷调查,结合相... 详细信息

为了综合评价甘肃省玛曲县在第二轮退牧还草工程(2005~2020年)实施中取得的成效,本文构建经济效益、生态效益、社会效益、工程的可持续性、工程的自身特性5个准则层、17个指标层的综合评价体系。采取随机抽样的方法展开问卷调查,结合相关部门意见构建层次分析-模糊综合评价模型,对实施成效进行评估。评估结果表明,自退牧还草工程实施以来,当地生态效益(0.4274)上升最为显著,其次是经济效益(0.2658)、社会效益(0.1693)、工程的可持续性(0.0838)和工程的自身特性(0.0564)。虽然退牧还草工程取得了显著的生态成效,但如何使成果持续有效地延续下去至关重要。通过项目实施前后指标层的权重分析,建议政府需加大与科研院所的合作力度,采取近自然修复技术,选择适合高寒环境的乡土草种补播,提高草场的生物多样性和稳定性;引领牧民构建畜产品产业链,促进第二、三产业发展,向以非传统牧业为主的经济收入模式迈进;加大对牧民文化教育事业的投入,提高牧民保护草原的生态意识;构建以草定畜、草畜同步发展的自然-人文耦合系统下的放牧模式和“减畜不减产、减畜不减收”的草地农业模式,促进草场可持续发展。以上研究结果有助于政府对退牧还草工程制定科学的规划和宏观的管理措施。

关键词：玛曲县退牧还草工程层次分析-模糊综合评价法成效评价

玉米茎腐病病原菌Fusarium graminearum拮抗真菌的筛选与鉴定

在线全文

学校读者我要写书评

暂无评论

吉林农业大学学报 2024年第1期46卷 34-39页

作者：赵子健王瑶张逸凡邓颖张丹王晓梅吉林农业大学植物保护学院长春130118

针对吉林省玉米茎腐病的主要优势致病菌禾谷镰孢菌进行拮抗菌株的筛选和鉴定,为玉米茎腐病生防菌剂的研制奠定基础。从300份土壤中分离出685个菌株,选取生长势较好的212个菌株,分别与禾谷镰孢菌进行平板对峙试验,筛选出拮抗效果较好的... 详细信息

针对吉林省玉米茎腐病的主要优势致病菌禾谷镰孢菌进行拮抗菌株的筛选和鉴定,为玉米茎腐病生防菌剂的研制奠定基础。从300份土壤中分离出685个菌株,选取生长势较好的212个菌株,分别与禾谷镰孢菌进行平板对峙试验,筛选出拮抗效果较好的拮抗菌株5株。将这5株菌进行抑菌试验,其中M-43号菌株抑菌率最高,为75.71%,选取M-43号真菌菌株进行后续研究。基于形态学显微观察和ITS序列分析,鉴定M-43号菌株为Talaromyces pinophilus,该种菌为吉林省新纪录种。

关键词：玉米茎腐病拮抗菌株 Fusarium graminearum Talaromyces pinophilus

Development of a dual temperature control system for isoprene biosynthesis in Saccharomyces cerevisiae

在线全文

学校读者我要写书评

暂无评论

Frontiers of Chemical Science and Engineering 2022年第7期16卷 1079-1089页

作者： Jiaxi Lin Zhen yao xiaomei Lyu Lidan Ye Hongwei Yu Institute of Bioengineering College of Chemical and Biological EngineeringZhejiang UniversityHangzhou 310027China Hangzhou Global Scientific and Technological Innovation Center Zhejiang UniversityHangzhou 311200China Key Laboratory of Systems Microbial Biotechnology Tianjin Institute of Industrial BiotechnologyChinese Academy of SciencesTianjin 300308China School of Food Science and Technology Jiangnan UniversityWuxi 214122China

Conflict between cell growth and product accumulation is frequently encountered in the biosynthesis of secondary metabolites. To address the growth-production conflict in yeast strains harboring the isoprene synthetic pathway in the mitochondria, the dynamic control of isoprene biosynthesis was explored. A dual temperature regulation system was developed through engineering and expression regulation of the transcriptional activator Gal4p. A cold-sensitive mutant, Gal4ep19, was created by directed evolution of Gal4p based on an internally developed growth-based high-throughput screening method and expressed under the heat-shock promoter PSSA4 to control the expression of PGAL-driven pathway genes in the mitochondria. Compared to the control strain with constitutively expressed wild-type Gal4p, the dual temperature regulation strategy led to 34.5% and 72% improvements in cell growth and isoprene production, respectively. This study reports the creation of the first cold-sensitive variants of Gal4p by directed evolution and provides a dual temperature control system for yeast engineering that may also be conducive to the biosynthesis of other high-value natural products.

关键词： transcriptional activator directed evolution dynamic control heat-shock isoprene

以功能康复为核心的护理模式对急性缺血性脑卒中取栓术后患者的影响

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

中外医学研究 2024年第5期22卷 74-78页

作者：凌素连董小梅岑瑶南宁市第二人民医院广西南宁530031

目的:探究以功能康复为核心的护理模式对急性缺血性脑卒中(AIS)取栓术后患者神经功能、肢体功能、并发症发生情况及生活质量的影响。方法:选取2021年12月—2023年4月南宁市第二人民医院神经内科收治的86例AIS取栓术后患者,按照护理方法... 详细信息

目的:探究以功能康复为核心的护理模式对急性缺血性脑卒中(AIS)取栓术后患者神经功能、肢体功能、并发症发生情况及生活质量的影响。方法:选取2021年12月—2023年4月南宁市第二人民医院神经内科收治的86例AIS取栓术后患者,按照护理方法分为对照组和观察组,各43例。其中对照组采用常规护理,观察组采用以功能康复为核心的护理模式。评估两组的神经功能、肢体功能、生活质量及并发症发生情况。结果:护理后,观察组美国国立卫生研究院卒中量表(NIHSS)评分及术后并发症发生率均低于对照组,差异有统计学意义(P<0.05);护理后,观察组Fugl-Meyer评估表(FMA)评分、运动功能评估量表(MAS)评分及健康调查简表(SF-36)中各个项目评分均高于对照组,差异有统计学意义(P<0.05)。结论:AIS患者取栓术后采用以功能康复为核心的护理模式有利于患者的神经功能、肢体功能的恢复,降低术后并发症的发生率,提升患者的生活质量。

关键词：功能康复为核心护理模式急性缺血性脑卒中取栓术生活质量

传统节日庆典与村落共同体意识的构建——以恭城石口村瑶族“花炮节”为例

在线全文

学校读者我要写书评

暂无评论

重庆文理学院学报（社会科学版） 2024年第2期43卷 24-31页

作者：彭小梅广西师范大学文学院广西桂林541000

花炮节是广西恭城瑶族自治县石口村最具地域性和民族性的传统节日庆典。花炮节主要内容包括还花炮、游花炮、文艺表演和抢花炮,是一个有祈福、庆祝、纪念、祭祀、社交等多重内涵和性质的综合性节日。花炮节承载着村落历史、民族记忆,有... 详细信息

花炮节是广西恭城瑶族自治县石口村最具地域性和民族性的传统节日庆典。花炮节主要内容包括还花炮、游花炮、文艺表演和抢花炮,是一个有祈福、庆祝、纪念、祭祀、社交等多重内涵和性质的综合性节日。花炮节承载着村落历史、民族记忆,有利于地理边界、社会边界的再确立;有利于推动传统伦理与现代观念、大传统与小传统之间的碰撞,实现村落秩序的整合;有利于利用文化资本,助力村落振兴,实现了文化共享。花炮节推动了村落边界的再确认、秩序的整合以及文化共享等多重目的实现,展现了村落共同体生成的实践路径。

关键词：花炮节节日庆典集体仪式村落共同体