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Matrix Stiffness-Induced Transcriptome Alterations and Regulatory Mechanisms Revealed by RNA-Seq in Endothelial Cells

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Journal of Biomaterials and Nanobiotechnology 2022年第3期13卷 61-79页

作者： Chao Zhang xiaomei han Jin Xu Yue Zhou School of Biomedical Engineering Shanghai Jiao Tong University Shanghai China

Changes in vascular stiffness are associated with the development and progression of many diseases, especially in cardiovascular disease. However, the effect of vascular stiffness on the endothelial cells (ECs) is not fully understood. Therefore, this study aims to determine the gene expression changes of ECs cultured on the matrices with different stiffness (1 kPa and 40 kPa, respectively) by RNA-seq, thereby broadening the knowledge between mechanics and biology. We obtained 1775 differentially expressed genes (DEGs) by RNA-seq, with 450 up-regulated and 1325 down-regulated DEGs in ECs cultured on soft matrix (1 kPa) compared to those cultured on stiff matrix (40 kPa). After that, we performed a series of functional enrichment analyses based on DEGs and found that DEGs were enriched in many signaling pathways like adhesion junction. Furthermore, transcription factor (TF) target gene prediction analysis and protein-protein interaction (PPI) analysis were also conducted. We found that mechanotransduction signaling related TFs such as BRD4 are involved in. And in the PPI analysis, some genes encoding extracellular matrix proteins such as fibronectin 1 (FN1) were identified as the hub genes. In order to confirm the RNA-seq results, we performed real-time qPCR analysis on the genes of interest, including FN1, collagen α2 (IV) chain, matrix metalloproteinase-14 and integrin α5, and found that the expression levels of all these genes were down-regulated on soft matrix, suggesting that soft matrix caused by pathological conditions may directly attenuate vascular barrier function. This study offers the insights about the effects of physical stimulation on cells, paving a way for vascular tissue engineering, regenerative medicine, disease modeling and therapies.

关键词： Endothelial Cells Matrix Stiffness RNA-Seq Cell-Cell Junctions Extracellular Matrix

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Microbiologically influenced corrosion of Cu by nitrate reducing marine bacterium Pseudomonas aeruginosa

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Journal of Materials Science & Technology 2020年第12期47卷 10-19页

作者： Yanan Pu Wenwen Dou Tingyue Gu Shiya Tang xiaomei han Department of Materials Science and Engineering Ocean University of ChinaQingdao 266100China Department of Chemical and Biomolecular Engineering Institute for Corrosion and Multiphase TechnologyOhio UniversityAthensOH 45701USA SINOPEC Research Institute of Safety Engineering Qingdao266071China Shandong Key Laboratory of Corrosion Science Institute of OceanologyChinese Academy of SciencesQingdao266071China

The microbiologically influenced corrosion(MIC) mechanisms of copper by Pseudomonas aeruginosa as a typical strain of nitrate reducing bacteria(NRB) was investigated in this lab study.Cu was immersed in deoxygenated LB-NO3 seawater inoculated with P.aeruginosa and incubated for 2 weeks.Results showed that this NRB caused pitting and uniform corrosion.The maximum pit depths after 7 d and 14 d in125 mL anaerobic vials with 50 mL broth were 5.1 μm and 9.1 μm,accompanied by specific weight losses of 1.3 mg/cm2(7 d) and 1.7 mg/cm2(14 d),respectively.Electrochemical measurements corroborated weight loss and pit depth data trends.Experimental results indicated that extracellular electron transfer for nitrate reduction was the main MIC mechanism and ammonia secreted by P.aeruginosa could also play a role in the overall Cu corrosion process.

关键词： Cu Pseudomonas aeruginosa Biofilm Nitrate reducing bacteria Microbiologically influenced corrosion Pitting corrosion

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Substrate Stiffness Affects Endothelial Cell Junctions and MAPK Signaling Pathway

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Journal of Biosciences and Medicines 2022年第2期10卷 124-135页

作者： Huanli Wang Chao Zhang xiaomei han Jin Xu Yue Zhou School of Biomedical Engineering Shanghai Jiao Tong University Shanghai China

Vascular homeostasis is critical for maintaining normal vascular structure and function. Aging is an irreversible trigger of vascular sclerosis, which causes structural and functional damage to blood vessels, leading to severe atherosclerosis. Endothelial cells (ECs) can respond to mechanical stimuli from the extracellular matrix, causing disruption of endothelial barrier function and activating signaling pathways to regulate cellular behavior under pathological conditions. In this paper, we investigated the effect of substrate stiffness on endothelial cell junctions, and the activation of mitogen-activated protein kinase (MAPK) signaling pathways. An in vitro stiffness model was established using polyacrylamide hydrogels of 1 kPa, 20 kPa and 100 kPa. By transcriptome analysis, we found that the cell-cell junction, cadherin binding, cytoskeleton and classical signaling pathways such as MAPK and Rho GTPase of endothelial cells were regulated by substrate stiffness. The expression of cell junction-related molecules TJP1, TJP2, JAM3 and JCAD was also found to be reduced at higher stiffness. The MAPK signaling pathway-related molecules MAP2K3, MAP2K7, MAP3K3, MAP3K6, MAPK3, MAPK7 were upregulated with increased stiffness. qRT-PCR analysis showed that the gene expression of JCAD was reduced with increased stiffness. Immunofluorescence staining of VE-cadherin indicated that the total fluorescence level of VE-cadherin decreased significantly with increased stiffness, and stiffness impaired the cell-cell junction with increased punctuation and discontinuity. Western blotting analysis confirmed that the protein expression ratio of pp38MAPK/p38MAPK increased with stiffness. Our research suggested that substrate stiffness played an important role in regulating endothelial cell integrity and MAPK signaling pathway.

关键词： Substrate Stiffness Endothelial Cell Cell Junction MAPK Signaling Pathway

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习近平文化思想引领中华优秀传统文化传承发展的三重论域

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河北科技大学学报（社会科学版） 2024年第2期24卷 1-7页

作者：韩晓梅李敏西华师范大学美术学院四川南充637002 西华师范大学马克思主义学院四川南充637002

习近平文化思想蕴含中华优秀传统文化传承发展的新思想新观点新论断,深刻论述了传承中华优秀传统文化的价值意蕴,科学阐明了弘扬中华优秀传统文化的思维方法,是新时代继承和弘扬中华优秀传统文化的思想指引与实践指南。以习近平文化思... 详细信息

习近平文化思想蕴含中华优秀传统文化传承发展的新思想新观点新论断,深刻论述了传承中华优秀传统文化的价值意蕴,科学阐明了弘扬中华优秀传统文化的思维方法,是新时代继承和弘扬中华优秀传统文化的思想指引与实践指南。以习近平文化思想为指引,挖深度,用中华民族最深沉的精神追求回答为何要传承中华优秀传统文化;讲高度,以国家战略资源的把握回复为何要弘扬中华优秀传统文化;拓广度,从推动中华民族现代化进程的角度思考实践理路,回应如何传承发展中华优秀传统文化。

关键词：习近平文化思想中华优秀传统文化价值意蕴思维方法实践理路

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The signaling pathway in modulating bone metabolism after dental implant in diabetes

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BIOCELL 2021年第6期45卷 1509-1519页

作者： xiaomei han SHUYING ZhanG YIFU WANG ChanGE QI PENGNYU GUO YALI XU GUANGHUI LYU Department of Oral Implantology Affiliated Hospital of Chifeng UniversityChifeng024000China

Diabetes Mellitus is a systematic disease with complications in multi-organs,including decreased implant osseointegration and a high failure rate of dental transplants.Accumulating evidence indicates that the signaling pathway directly impacts the process of bone metabolism and inflammatory response implicated with dental implants in diabetic patients.This review summarizes the recent advance in signaling pathways regulate osseointegration and inflammatory response in dental transplantation,aiming to identify the potential therapeutic target to reduce the dental transplant failure in diabetes patients,with emphasis on the surface characteristics of the implant,inflammatory signaling,AMPK,PPARγ,WNT,ROS,and adiponectin signaling.

关键词： Diabetes Osseointegration Titanium Wnt ROS Adiponectin PPARγ Dental transplant

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政府印刷业务预算绩效管理需要印刷企业同步

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印刷杂志 2024年第3期 20-24页

作者：李保强韩小梅河北凡芃资产评估咨询有限公司

政府性的印刷业务是一块重要的市场,成为政府定点印刷企业也是企业市场形象的展现,历来为印刷企业所重视。印刷品是行政事业单位履行部门职能过程中的一种必不可少的物料,不仅包括日常办公印刷品,也有针对专项工作的印刷品,如证书、宣... 详细信息

政府性的印刷业务是一块重要的市场,成为政府定点印刷企业也是企业市场形象的展现,历来为印刷企业所重视。印刷品是行政事业单位履行部门职能过程中的一种必不可少的物料,不仅包括日常办公印刷品,也有针对专项工作的印刷品,如证书、宣传品等。

关键词：政府印刷业务绩效管理绩效评价

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匐行性回状红斑样荨麻疹性血管炎1例国内首报

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中国皮肤性病学杂志 2024年第3期38卷 329-331页

作者：张双马静张燕王学良韩晓梅杜明河北医科大学第四医院皮肤科河北石家庄050011

患者男,57岁,全身出现环状红斑,伴瘙痒和疼痛5 d。皮肤科情况:面部、躯干及四肢可见水肿性同心圆形红斑,呈木纹状外观,边缘隆起,未见明显鳞屑,全身浅表淋巴结未触及肿大。皮损组织病理示:白细胞碎裂性血管炎,真皮浅层水肿明显,胶原纤维... 详细信息

患者男,57岁,全身出现环状红斑,伴瘙痒和疼痛5 d。皮肤科情况:面部、躯干及四肢可见水肿性同心圆形红斑,呈木纹状外观,边缘隆起,未见明显鳞屑,全身浅表淋巴结未触及肿大。皮损组织病理示:白细胞碎裂性血管炎,真皮浅层水肿明显,胶原纤维束间隙增宽;血管内皮肿胀,血管壁纤维蛋白样变性不明显;真皮浅层及血管周围可见以淋巴细胞为主的炎细胞浸润,少许红细胞外渗。根据患者临床表现和组织病理学检查,诊断为匐行性回状红斑样荨麻疹性血管炎。给予降低毛细血管通透性等治疗,皮疹基本消退,仅残留淡褐色色素沉着斑。患者出院4个月后随访皮疹未再复发。

关键词：匐行性回状红斑荨麻疹性血管炎首报

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表现为面部孤立结节的传染性软疣

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临床皮肤科杂志 2024年第2期53卷 67-68页

作者：胡彩霞赵璐韩晓梅崔瑜程毅王文氢河北医科大学第四医院皮肤科河北石家庄050000

患者女,19岁。主诉:右侧颞部结节4个月。现病史:患者4个月前右侧颞部出现米粒大丘疹,缓慢增大,未发生破溃,无疼痛及瘙痒,于2020年10月来我科就诊。既往史、个人史及家族史:无特殊。皮肤科检查:右侧颞部一黄豆大肤色结节,表面光滑,界限清... 详细信息

患者女,19岁。主诉:右侧颞部结节4个月。现病史:患者4个月前右侧颞部出现米粒大丘疹,缓慢增大,未发生破溃,无疼痛及瘙痒,于2020年10月来我科就诊。既往史、个人史及家族史:无特殊。皮肤科检查:右侧颞部一黄豆大肤色结节,表面光滑,界限清楚(图1),质韧,表面有针尖样小孔。

关键词：软疣传染性

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A semantic segmentation-based underwater acoustic image transmission framework for cooperative SLAM

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Defence Technology（防务技术） 2024年第3期33卷 339-351页

作者： Jiaxu Li Guangyao han Shuai Chang xiaomei Fu School of Marine Science and Technology Tianjin UniversityTianjin 300072China

With the development of underwater sonar detection technology,simultaneous localization and mapping(SLAM)approach has attracted much attention in underwater navigation field in recent years.But the weak detection ability of a single vehicle limits the SLAM performance in wide areas.Thereby,cooperative SLAM using multiple vehicles has become an important research direction.The key factor of cooperative SLAM is timely and efficient sonar image transmission among underwater vehicles.However,the limited bandwidth of underwater acoustic channels contradicts a large amount of sonar image data.It is essential to compress the images before transmission.Recently,deep neural networks have great value in image compression by virtue of the powerful learning ability of neural networks,but the existing sonar image compression methods based on neural network usually focus on the pixel-level information without the semantic-level information.In this paper,we propose a novel underwater acoustic transmission scheme called UAT-SSIC that includes semantic segmentation-based sonar image compression(SSIC)framework and the joint source-channel codec,to improve the accuracy of the semantic information of the reconstructed sonar image at the receiver.The SSIC framework consists of Auto-Encoder structure-based sonar image compression network,which is measured by a semantic segmentation network's residual.Considering that sonar images have the characteristics of blurred target edges,the semantic segmentation network used a special dilated convolution neural network(DiCNN)to enhance segmentation accuracy by expanding the range of receptive fields.The joint source-channel codec with unequal error protection is proposed that adjusts the power level of the transmitted data,which deal with sonar image transmission error caused by the serious underwater acoustic channel.Experiment results demonstrate that our method preserves more semantic information,with advantages over existing methods at the same compression ratio.It also improves the error

关键词： Semantic segmentation Sonar image transmission Learning-based compression

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1例犬后肢瘫痪的中西医诊治报告

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现代畜牧兽医 2024年第2期 53-56页

作者：任杰肖红严易高俊波韩晓梅桂国宏铜仁职业技术学院贵州铜仁550025 铜仁市中医院贵州铜仁550025 贵州农业职业学院贵州贵阳551400

犬后肢瘫痪主要是由椎间盘损伤、神经阻滞、代谢紊乱等因素作用使动物后肢失去运动功能的病症,常采用手术和激素进行治疗,但效果不理想。文章介绍了一例犬后肢瘫痪的中西医结合诊治过程。通过临床症状观察、血常规、血液生化检查、C-反... 详细信息

犬后肢瘫痪主要是由椎间盘损伤、神经阻滞、代谢紊乱等因素作用使动物后肢失去运动功能的病症,常采用手术和激素进行治疗,但效果不理想。文章介绍了一例犬后肢瘫痪的中西医结合诊治过程。通过临床症状观察、血常规、血液生化检查、C-反应蛋白检查、血气分析及影像学检查,对该病例进行综合确诊。采用支持疗法、针灸、穴位注射神经营养液及辅助康复练习进行治疗,患犬痊愈。

关键词：犬后肢瘫痪针灸穴位

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