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社会文化理论视角下翻译硕士专业研究生翻译实践能动性研究

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北京化工大学学报（社会科学版） 2024年第1期 128-135页

作者：孙晓曼张庆华中国地质大学(北京)外国语学院北京100083

翻译硕士专业学位注重培养学生的实践能力,学生的翻译实践能动性对于提高其翻译能力和翻译实践效果起着关键作用,但目前相关研究较少。本研究基于社会文化理论,采用定量分析与定性分析相结合的研究方法,探究翻译硕士研究生翻译实践能动... 详细信息

翻译硕士专业学位注重培养学生的实践能力,学生的翻译实践能动性对于提高其翻译能力和翻译实践效果起着关键作用,但目前相关研究较少。本研究基于社会文化理论,采用定量分析与定性分析相结合的研究方法,探究翻译硕士研究生翻译实践能动性的维度和水平。研究发现:(1)翻译硕士研究生的翻译实践能动性可划分为情感认同、过程控制和自我驱动三个维度;(2)64.9%的学生翻译实践能动性总水平偏低,且翻译实践能动性三个维度间存在显著差异,其中自我驱动维度得分最低。本文可为创新翻译实践研究视角、提高翻译硕士研究生翻译实践质量提供参考。

关键词：翻译硕士专业研究生能动性翻译实践社会文化理论

人GⅡ.14诺如病毒VLP抗原性及组织血型抗原结合特征

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病毒学报 2024年第4期40卷 705-715页

作者：欧阳瑄泽柴鹏弟宋敬东靳淼李利利李金松孙晓曼段招军甘肃中医药大学公共卫生学院兰州730020 中国疾病预防控制中心病毒病预防控制所传染病溯源预警与智能决策全国重点实验室国家卫生健康委员会医学病毒和病毒病重点实验室北京102206

本研究旨在制备人GⅡ.14诺如病毒的(Norovirus,NoV)病毒样颗粒(Virus⁃like particle,VLP),探索其抗原性与组织血型抗原结合的特征。利用杆状病毒系统表达人GⅡ.14 NoV VP1蛋白,通过超速离心和分子筛层析纯化VLP;将VLP免疫动物制备兔多... 详细信息

本研究旨在制备人GⅡ.14诺如病毒的(Norovirus,NoV)病毒样颗粒(Virus⁃like particle,VLP),探索其抗原性与组织血型抗原结合的特征。利用杆状病毒系统表达人GⅡ.14 NoV VP1蛋白,通过超速离心和分子筛层析纯化VLP;将VLP免疫动物制备兔多抗血清,通过免疫印迹(Western blot,WB)、酶联免疫吸附(ELISA)进行抗原性评价及多抗功能验证;利用唾液结合实验检测人GⅡ.14 VLP的糖结合特征;通过蛋白序列及结构比较分析GⅡ.14 P蛋白潜在的糖结合位。纯化获得人GⅡ.14 VP1蛋白,电镜观察显示可以形成结构较为均一的VLP;制备的兔多抗能与GⅡ.14 VLP特异性结合,与检测的其他基因型VLP没有交叉反应;唾液结合实验表明GⅡ.14 VLP与人A/B/O/AB型别唾液都有较好的结合;序列和结构分析显示GⅡ.14 P蛋白模拟结构与GⅡ.9最为接近,具有与GⅡ.9以及流行株GⅡ.4等相似的潜在糖结合区。本研究表明GⅡ.14具有较为广泛的唾液结合特性,通过序列比对和结构学方法分析了GⅡ.14潜在的受体结合机制,为不同型别NoVs的流行监测提供了更多依据。

关键词：诺如病毒病毒样颗粒组织血型抗原

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CircR-ZC3HC1 mediates MiR-384-5p/SIRT1 axis to promote neuronal autophagy and relieves ischemic stroke

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BIOCELL 2024年第3期48卷 491-499页

作者： MIN SHEN xiaoman XU GUANGLING sun LIANGZHU WANG TAO YING HANG SU WEI WANG QINGHUA CAO ZHEZHE sun Department of Neurology The First Affiliated Hospital of Ningbo UniversityNingbo315010China School of Medicine Ningbo UniversityNingbo315211China

Objective:Circular RNAs(circRNAs)have been shown to involve in pathological processes of ischemic stroke(IS),including *** study was designed to explore the effect of circR-ZC3HC1 on neuronal autophagy in IS and the related ***:Expression of circR-ZC3HC1 in blood samples of IS patients and healthy controls was *** neurons were treated with oxygen and glucose deprivation(OGD)to establish IS in vitro *** expression of LC3 and p62 and the number of autophagosomes were examined to evaluate the autophagy level of OGD induced neurons using western blotting and transmission electron *** apoptosis rate and the expression of cleaved caspase-3,Bax,and Bcl-2 were assessed byﬂow cytometry and western *** binding relationships among circR-ZC3HC1,miR-384-5p,and SIRT1 were predicted and veriﬁ***:Low expression of circR-ZC3HC1 was found in blood samples of IS patients and OGD-treated *** circR-ZC3HC1 or inhibited miR-384-5p expression promoted autophagy and inhibited apoptosis of OGD-treated neurons,which could be reversed by further 3-MA ***,circR-ZC3HC1 targeted miR-384-5p to mediate SIRT1 ***-384-5p overexpression or SIRT1 knockdown in the presence of circR-ZC3HC1 overexpression in OGD-treated neurons lead to reduced autophagy and enhanced ***:Collectively,circR-ZC3HC1 promoted neuronal autophagy to attenuate IS via miR-384-5p/SIRT1 axis.

关键词： Ischemic stroke circR-ZC3HC1 miR-384-5p SIRT1 Autophagy

Styrax japonicus functional genomics:an efficient virus induced gene silencing(VIGS)system

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Horticultural Plant Journal 2024年第1期10卷 252-258页

作者： Gangyu sun Yiqian Ju Cuiping Zhang Lulu Li Xinqiang Jiang xiaoman Xie Yizeng Lu Kuiling Wang Wei Li College of Landscape Architecture and Forestry Qingdao Agricultural UniversityQingdaoShandong 266109China Shandong Provincial Center of Forest and Grass Germplasm Resources JinanShandong 250102China

VIGS(Virus-induced gene silencing),a method for posttranscriptional gene silencing,is an effective technique for investigating the activities of genes in *** there is no report for available VIGS system in Styrax japonicus,the application of a VIGS approach that results in a gene knockdown to study gene function is *** this study,we compared the characteristics that could affect the viability of VIGS in ***,including the acetosyringone(AS)concentration,the Agrobacterium’s optical density and the inoculation *** stable reference genes of *** were selected to validate the gene’s knockdown by quantitative *** a result,we successfully constructed 2 VIGS systems based on TRV virus:vacuum with AS concentration of 200μmol·L^(-1)and OD600of 0.5,and friction-osmosis with AS concentration of 200μmol·L^(-1)and OD600of 1.0,which silencing efficiency was 83.33%and 74.19%,*** successfully applied VIGS method provides a rapid and effective reverse gene functional analysis approach in *** to identify unknown gene functions.

关键词： Styrax japonicus VIGS Reference gene

艾司氯胺酮联合丙泊酚在儿童腹股沟疝手术的应用效果分析

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齐齐哈尔医学院学报 2024年第12期45卷 1127-1131页

作者：孙晓满杨铎胡锦钰谢贵彬林旭林陈哲璇张隆盛揭阳市人民医院麻醉科广东揭阳522000

目的探讨艾司氯胺酮联合丙泊酚在儿童腹股沟疝手术中的应用效果。方法选择2022年11月—2023年6月本院收治的拟行传统疝囊高位结扎术的住院患儿96例作为研究对象,年龄1~6岁,ASAⅠ~Ⅱ级。采用随机数表法将本院收治的患儿分为艾司氯胺酮组(... 详细信息

目的探讨艾司氯胺酮联合丙泊酚在儿童腹股沟疝手术中的应用效果。方法选择2022年11月—2023年6月本院收治的拟行传统疝囊高位结扎术的住院患儿96例作为研究对象,年龄1~6岁,ASAⅠ~Ⅱ级。采用随机数表法将本院收治的患儿分为艾司氯胺酮组(E组)、芬太尼组(F组)和丙泊酚组(P组),每组各32例。E组、F组和P组分别在术者铺巾完成后静脉注射0.5 mg/kg艾司氯胺酮、1μg/kg芬太尼和1 mg/kg丙泊酚,5 min后用0.5%利多卡因1 mg/kg在手术侧行局麻后开始手术。每组均在入室后静脉持续泵注丙泊酚9~15 mg/(kg·h),术中根据患者体动情况及生命体征变化情况增减丙泊酚的泵注速度。记录入室前(T_(0))、试验药物给药后5 min(T_(1))、手术开始时(T_(2))、手术结束时(T_(3))、入恢复室时(T_(4))和患者苏醒时(T_(5))的心率(HR)、平均动脉压(MAP)。记录术中丙泊酚泵注剂量、手术时间、苏醒时间、恢复室滞留时间以及苏醒期躁动和疼痛评分(PAED评分)。记录三组呼吸抑制、苏醒期躁动、恶心呕吐、不自主体动等不良反应发生情况。结果最终完成研究92例,与E组相比,F组和P组丙泊酚泵注剂量显著减少(P0.05)。结论0.5 mg/kg艾司氯胺酮联合丙泊酚用于儿童腹股沟疝手术,血流动力学稳定,不仅可降低丙泊酚的泵注剂量,而且可降低呼吸抑制和不自主体动等不良反应的发生率。

关键词：艾司氯胺酮丙泊酚芬太尼儿童腹股沟疝手术

Imide-pillared covalent organic framework protective films as stable zinc ion-conducting interphases for dendrite-free Zn metal anodes

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Journal of Energy Chemistry 2024年第10期97卷 470-477页

作者： xiaoman Ye Xuemei Xiao Zhijing Wu Xin Wu Lin Gu Sheng Liu School of Chemical Engineering and Technology Sun Yat-sen University The Key Lab of Low-Carbon Chemistry & Energy Conservation of Guangdong Province Sun Yat-sen University

The notorious growth of zinc dendrite and the water-induced corrosion of zinc metal anodes（ZMAs）restrict the practical development of aqueous zinc ion batteries（AZIBs）.In this work,a zinc metallized,imide-pillared covalent organic framework（ZPC） protective film has been engineered as a stable Zn2+ion-conducting interphase to modulate interfacial kinetics and suppress side reactions for *** to bare Zn,ZPC@Zn exhibits a higher Zn2+ionic conductivity,a larger Zn2+transference number,a lower electronic conductivity,a smaller desolvation activation energy and correspondingly a significant suppression of corrosion,hydrogen evolution and Zn ***,the ZPC@Zn||ZPC@Zn symmetric cell obtains a cycling lifespan over 3000 h under 5 mA cm-2for 1 mA h *** ZPC@Zn||NH4V4O10coin-type full battery delivers a specific capacity of 195.8 mA h g-1with a retention rate of78.5% at 2 A g-1after 1100 cycles,and the ZPC@Zn||NH4V4O10pouch full cell shows a retention of70.1% in reversible capacity at 3 A g-1after 1100 *** present incorporation of imide-linked covalent organic frameworks in the surface modification of ZMAs will offer fresh perspectives in the search for ideal protective films for the practicality of AZIBs.

关键词： Aqueous zinc ion batteries Covalent organic frameworks Imide linkage Surface modification Zinc metal anodes

一株罕见G4P[6]-I5-A8基因型人轮状病毒全基因组序列分析

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国际病毒学杂志 2024年第1期31卷 17-21页

作者：车如意周杰英范佳欣唐晓苹董梦洁熊光萍孙晓曼王宏章青庞立丽孔翔羽李丹地传染病溯源预警与智能决策全国重点实验室、中国疾病预防控制中心病毒病预防控制所、国家卫生健康委员会医学病毒和病毒病重点实验室北京102206 郴州市第一人民医院 423000

目的研究一株G4P[6]基因型A组轮状病毒(group A rotavirus,RVA)CZ079的全基因组特征及进化规律.方法利用逆转录-聚合酶链式反应(reverse transcription-polymerase chain reaction,RT-PCR)对CZ079的VP7、VP4、VP6、NSP1节段分别进行RT-PCR扩增,通过在线RVA自动分型工具对其分型,采用DNAstar5.1和Mega 11.0软件对各节段进行同源性及遗传进化分析.结果CZ079毒株基因型为G4-P[6]-I5-R ***6为I5型,NSP1为A8型.CZ079与LLR^(TM)、RotaTeq^(TM)和Rotarix^(TM)三种疫苗株在VP7的抗原表位中7-1b区域均存在氨基酸差异;VP4蛋白裂解后的VP8*抗原表位中8-1、8-2、8-3、8-4、5-1区域存在较大差异.结论2022年RVA毒株CZ079为罕见的G4P[6]-I5-A8型,推测该基因型RVA是由越南等东南亚地区传入中国,与中国RVA发生重配产生的新型重配毒株.

关键词： A组轮状病毒基因型重配株全基因组抗原表位

Polyfunctional Conjugated Microporous Polymers for Applications in Direct C—H Arylation of Unactivated Arenes and Aqueous Adsorption of Aromatic Amines

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Chemical Research in Chinese Universities 2020年第6期36卷 1302-1309页

作者： LUO Kexin SU Qing JU Pengyao LI Xiaodong LIU Ziqian sun xiaoman LI Guanghua WU Qiaolin College of Chemistry Jilin UniversityChangchun 130012P.R.China State Key Laboratory of Inorganic Synthesis and Preparative Chemistry Jilin UniversityChangchun 130012P.R.China

Salen-porphyrin-based conjugated microporous polymers(CMPs)have been demonstrated to be an attractive material platform for predesigned structures and promising ***,a new salen-porphyrin-based conjugated microporous polymer(SP-CMP-L)was solvothermally prepared by porphyrin-forming condensation reaction of pyrrole and salen-dialdehyde *** SP-CMP-L was characterized by spectroscopy technologies,and also exhibited excellent thermal and chemical *** porosity of SP-CMP-L was examined by N2 adsorption/desorption *** BET specific surface area of the CMP material was calculated to be 290.4 m^2/g with the pore volume of 0.19 cm^3/*** microstructure property of the resulting material was further evaculated by scanning electron microscopy(SEM)and transmission electron microscopy(TEM).The SP-CMP-L with salen and porphyrin multidentate coordination sites was proposed to serve as an initiator to promote the cross-coupling between aryl halides with unactivated arenes under base mediated *** transition-metal-free catalytic protocol provided high catalytic activity for direct C-H arylation reaction of unactivated arenes,and thus offered a convenient and efficient alternative for the construction biaryl *** addition,the salen-porphyrin based CMP material possessed remarkable adsorption capability for the removal of organic amines from water.

关键词： Conjugated microporous polymer C-H arylation Aqueous adsorption

Design of high-performance and sustainable Co-free Ni-rich cathodes for next-generation lithium-ion batteries

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SusMat 2024年第1期4卷 48-71页

作者： Hao Ge Zhiwen Shen Yanhong Wang Zhijia sun xiaoman Cao Chaoyue Wang Xinyue Fan Jinsong Bai Rundong Li Tianhua Yang Gang Wu School of Energy and Environment Shenyang Aerospace UniversityShenyangP.R.China Shenyang Aircraft Airworthiness Certification Center of CAAC ShenyangP.R.China College of Chemistry and Materials Engineering Bohai UniversityJinzhouP.R.China Liaoning General Aviation Academy ShenyangP.R.China Department of Chemical and Biological Engineering University at BuffaloState University of New YorkBuffaloNew YorkUSA

Great attention has been given to high-performance and inexpensive lithiumion batteries(LIBs)in response to the ever-increasing demand for the explosive growth of electric vehicles(EVs).High-performance and low-cost Co-freeNi-rich layered cathodes are considered one of the most favorable candidates for nextgeneration LIBs because the current supply chain of EVs relies heavily on scarce and expensive ***,we review the recent research progress on Co-free Nirich layered cathodes,emphasizing on analyzing the necessity of replacing Co and the popular improvment *** current advancements in the design strategies of Co-free Ni-rich layered cathodes are summarized in *** considerable improvements achieved so far,the main technical challenges contributing to the deterioration of Co-free Ni-rich cathodes such as detrimental phase transitions,crack formation,and severe interfacial side reactions,are difficult to resolve by a single *** cooperation of multiple modification strategies is expected to accelerate the industrialization of Co-free Ni-rich layered cathodes,and the corresponding synergistic mechanisms urgently need to be *** effects will be aroused to explore high-performance Co-free Ni-rich layered cathodes to promote the sustainable development of LIBs.

关键词： Co-free cathodes electrochemical performance lithium-ion batteries modification strategies Ni-rich layered cathodes