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Mitochondrial transplantation confers protection against the effects of ischemic stroke by repressing microglial pyroptosis and promoting neurogenesis

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Neural Regeneration Research 2024年第6期19卷 1325-1335页

作者： Li Sun Zhaoyan Zhao Jing Guo Yuan Qin Qian Yu xiaolong shi Fei Guo Haiqin Zhang Xude Sun Changjun Gao Qian Yang Department of Experimental Surgery The Second Affiliated Hospital of Air Force Medical University Department of Anesthesiology The Second Affiliated Hospital of Air Force Medical University

Transferring healthy and functional mitochondria to the lateral ventricles confers neuroprotection in a rat model of ischemia-reperfusion injury. Autologous mitochondrial transplantation is also beneficial in pediatric patients with cardiac ischemia-reperfusion injury. Thus, transplantation of functional exogenous mitochondria may be a promising therapeutic approach for ischemic disease. To explore the neuroprotective effect of mitochondria transplantation and determine the underlying mechanism in ischemic stroke, in this study we established a photo-thrombosis-induced mouse model of focal ischemia and administered freshly isolated mitochondria via the tail vein or to the injury site(in situ). Animal behavior tests, immunofluorescence staining, 2,3,5-triphenyltetrazolium chloride(TTC) staining, mRNA-seq, and western blotting were used to assess mouse anxiety and memory, cortical infarct area, pyroptosis, and neurogenesis, respectively. Using bioinformatics analysis, western blotting, co-immunoprecipitation, and mass spectroscopy, we identified S100 calcium binding protein A9(S100A9) as a potential regulator of mitochondrial function and determined its possible interacting proteins. Interactions between exogenous and endogenous mitochondria, as well as the effect of exogenous mitochondria on recipient microglia, were assessed in vitro. Our data showed that:(1) mitochondrial transplantation markedly reduced mortality and improved emotional and cognitive function, as well as reducing infarct area, inhibiting pyroptosis, and promoting cortical neurogenesis;(2) microglial expression of S100A9 was markedly increased by ischemic injury and regulated mitochondrial function;(3) in vitro, exogenous mitochondria enhanced mitochondrial function, reduced redox stress, and regulated microglial polarization and pyroptosis by fusing with endogenous mitochondria; and(4) S100A9 promoted internalization of exogenous mitochondria by the microglia, thereby amplifying their pro-prolifera

关键词： ischemic stroke microglia mitochondria transplantation neurogenesis pyroptosis

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Priority Encoder Based on DNA Strand Displacement

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Chinese Journal of Electronics 2024年

作者： Fang WANG Xinjian ZHANG Xin CHEN Shuying Lü Congzhou CHEN xiaolong shi Institution of Computing Science and Technology Guangzhou University Beijing University of Technology School of Computer Science Peking University

The slow development of traditional computing has prompted a search for new materials to replace silicon-based computers. Bio-computers, which use molecules as the basis of computation, are highly parallel and information capable, attracting a lot of attention. In this study, we designed a NAND logic gate based on the DNA strand displacement mechanism. We assembled a molecular calculation model, a 4-wire-2-wire priority encoder logic circuit, by cascading the proposed NAND gates. Different concentrations of input DNA chains were added into the system, resulting in corresponding output, through DNA hybridization and strand displacement. Therefore, it achieved the function of a priority encoder. Simulation results verify the effectiveness and accuracy of the molecular NAND logic gate and the priority coding system presented in this study. The unique point of this proposed circuit is that we cascaded only one kind of logic gate, which provides a beneficial exploration for the subsequent development of complex DNA cascade circuits and the realization of the logical coding function of information.

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普遍诠释学视域下高职思政理论课有效性提升的机理、目标与路径

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中国职业技术教育 2024年第26期 18-24页

作者：施小龙杜东芳重庆幼儿师范高等专科学校

高职思政理论课是塑造高职学生正确三观,培养学生崇高思想品质的关键课程。引导高职学生对思政理论课程文本原义达成理解,对课程文本蕴含思想的领悟、内化、践行是高职思政理论课有效性的内在目标。当前高职院校思政理论课教学中,存在... 详细信息

高职思政理论课是塑造高职学生正确三观,培养学生崇高思想品质的关键课程。引导高职学生对思政理论课程文本原义达成理解,对课程文本蕴含思想的领悟、内化、践行是高职思政理论课有效性的内在目标。当前高职院校思政理论课教学中,存在学生对课程文本语言理解不深、通过语言而领悟思想不透、以思想引领行动的实践不强等低效性表征。借鉴普遍诠释学相关理念,充分调动学生认知“前结构”,引导学生积极对思政理论课的课程文本进行“语法诠释”和“心理诠释”,从而达成深刻领悟课程文本蕴含思想内涵的目标,进而形成提升高职院校思政理论课有效性的合理路径。

关键词：普遍诠释学高职思政理论课

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淹没式移动射流对黏性底床开沟试验研究

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海洋工程 2024年第2期42卷 115-124页

作者：陈凌峰赵林石晓龙邹星中国海洋大学工程学院山东青岛266100 中海石油(中国)有限公司北京研究中心北京100028

以试验方法研究双喷式射流结构对软质黏性底床上可沉降管道移动射流开挖规律。通过黄土、高岭土、水泥配置低剪切强度试验黏土,揭示喷嘴射流速度、喷嘴平移速度对冲坑特征尺寸影响规律。以边界层剪应力分析冲坑形态变化,并引入低射流靶... 详细信息

以试验方法研究双喷式射流结构对软质黏性底床上可沉降管道移动射流开挖规律。通过黄土、高岭土、水泥配置低剪切强度试验黏土,揭示喷嘴射流速度、喷嘴平移速度对冲坑特征尺寸影响规律。以边界层剪应力分析冲坑形态变化,并引入低射流靶距下反映侵蚀能力强度的回归侵蚀特征值Z进行无量纲化分析。结果表明:双喷式垂直射流形成的冲坑断面轮廓具有中心对称性,移动射流形成的冲坑纵向剖面线具有几何相似性。冲坑特征尺寸随射流速度呈近似线性变化,随喷嘴平移速度增加呈减小趋势。剪切强度增加使得黏土抗侵蚀能力增强,冲坑稳定断面曲线由V形向U形逐渐过渡变化。

关键词：黏性土壤土体剪切强度移动射流射流侵蚀冲坑特征尺寸

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Reversible spiking neural P systems

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Frontiers of Computer Science 2013年第3期7卷 350-358页

作者： Tao SONG xiaolong shi Jinbang XU Key Laboratory of Image Processing and Intelligent Control Department of Control Science and Engineering Huazhong University of Science and Technology Wuhan 430074 China

Spiking neural （SN） P systems are a class of distributed parallel computing devices inspired by the way neurons communicate by means of spikes. In this work, we investigate reversibility in SN P systems, as well as the computing power of reversible SN P systems. Reversible SN P systems are proved to have Turing creativity, that is, they can compute any recursively enumerable set of non-negative integers by simulating universal reversible register machine.

关键词： membrane computing spiking neural P system reversible computing model universality reversible register machine

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行为表象和感性再造:基于文化符号学理论的传统武术文化研究

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武术研究 2024年第7期9卷 33-36页

作者：卫微石晓龙山西农业大学山西太谷030800

为研究传统武术的文化符号性,文章运用文献资料法与逻辑分析法,以文化符号学的基本理论为基础,深入剖析传统武术的文化符号特征。研究认为,文化符号学理论适用于解释传统武术的文化符号特征;传统武术符号是传统武术文化在传播中的身体... 详细信息

为研究传统武术的文化符号性,文章运用文献资料法与逻辑分析法,以文化符号学的基本理论为基础,深入剖析传统武术的文化符号特征。研究认为,文化符号学理论适用于解释传统武术的文化符号特征;传统武术符号是传统武术文化在传播中的身体技术载体和文化内涵共同组成的符号系统,在形式上体现为传统武术文化的行为表象,在内容上体现为习练者对其文化的感性再造;传统武术文化符号的形式具有依赖身体媒介的不确定性,演变出丰富符号的特点,并由此促进传统武术身体技术的创造,同时,因符号形式的代际传承,使它拥有了符号交流和保存中具有的审美普遍性,并具有符号选择的任意性特征;传统武术文化符号的内容在能指与所指的传递过程中,依靠个体的感性认识和再造实现所指内容的无限丰富,同时,社会群体对个体的规范作用,使符号内容具有个体与群体的双重维度特征和在符号内容上具有思想教化的人格改造功能。

关键词：文化符号传统武术形式内容特质

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DNA sequencing by synthesis with degenerate primers

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Journal of Genetics and Genomics 2008年第9期35卷 545-551页

作者： Chao Tang xiaolong shi Xiujie Li Zuhong Lu State Key Laboratory of Bioelectronics Southeast University Nanjing 210096 China The School of Basic Medical Sciences Southeast University Nanfing 210096 China

The degenerate primer-based sequencing was developed by a synthesis method （DP-SBS） for high-throughput DNA sequencing, in which a set of degenerate primers are hybridized on the arrayed DNA templates and extended by DNA polymerase on microarrays. In this method, a different set of degenerate primers containing a given number （n） of degenerate nucleotides at the 3＇-ends were annealed to the sequenced templates that were immobilized on the solid surface. The nucleotides （n＋1） on the template sequences were determined by detecting the incorporation of fluorescent labeled nucleotides. The fluorescent labeled nucleotide was incorporated into the primer in a base-specific manner after the enzymatic primer extension reactions and nine-base length were read out accurately. The main advantage of the DP-SBS is that the method only uses very conventional biochemical reagents and avoids the complicated special chemical reagents for removing the labeled nucleotides and reactivating the primer for further extension. From the present study, it is found that the DP-SBS method is reliable, simple, and cost-effective for laboratory-sequencing a large amount of short DNA fragments.

关键词： microarrays DNA sequencing degenerate primer extension SBS DP-SBS

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Simulation of ChIP-Seq based on extra-sonication of IPed DNA fragments

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Chinese Science Bulletin 2010年第22期55卷 2380-2389页

作者： WANG Wei shi xiaolong LU ZuHong State Key Laboratory of Bioelectronics School of Biological Science and Medical Engineering Southeast University Nanjing 210096 China

The combination of chromatin immunoprecipitation with sequencing (ChIP-Seq) is an effective method for obtaining an in vivo genome-wide profile of the interaction of a protein with DNA. With the dramatic development of high-throughput short sequencing technologies, several new algorithms have been developed to process ChIP-Seq. However, the reported analytical tools for ChIP-Seq based on size selection of immunoprecipitated (IPed) DNA fragments are mainly adopted on the Solexa system. As a sequencer with the highest throughput, few studies of ChIP-Seq based on SOLiD system have been reported. The main difference of the SOLiD and Solexa systems exists in the length of DNA fragments during preparing sequencing libraries. The SOLiD system has relatively short DNA fragments if it processes a further sonication of IPed DNA fragments in order to meet the length requirement of DNA fragments for emulsion-PCR (ePCR). This work aims to investigate the influences of DNA fragment length on data analysis from ChIP-Seq. Previous studies show that typical bimodal peaks can be observed in Solexa ChIP-Seq data, but based on the analysis of the real SOLiD ChIP-Seq data in this study, we found that there were no double peaks with apparent reads shift in a local enriched region and the local reads distribution of peaks were tested by normal distribution. Using real and simulated ChIP-Seq data, three main ChIP-Seq algorithms (CisGenome, SISSRs and MACS) have been investigated. We found that algorithms developed for processing ChIP-Seq data generated from Solexa library protocol, cannot efficiently capture the feature of the ChIP-Seq data from SOLiD library. Misuse of those analytical tools would be a possible reason for failure of ChIP-Seq on the SOLiD system. Therefore, a new ChIP-Seq analytical strategy for an extra-sonication of IPed DNA fragments needs to be developed.

关键词： DNA片段超声模拟染色质免疫沉淀 iD系统芯片片段长度聚合酶链反应

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2种樟树叶片基质添加对牛樟芝三萜含量及抗氧化活性影响

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西南林业大学学报（自然科学） 2024年第5期44卷 120-128页

作者：郑洋群尹以杭余思廷赵晓雷石晓龙郑元西南林业大学林学院云南昆明650233 西南林业大学云南省森林灾害预警与控制重点实验室云南昆明650233

比较分析在大米培养基中添加2种樟树不同浓度叶片匀浆对牛樟芝三萜含量及抗氧化活性的影响,筛选出最适的培养条件,为牛樟芝人工培养及樟属植物资源开发利用提供参考。以大米培养基为对照,分别添加0、0.5、1、2、3、4 g共6个浓度的牛樟... 详细信息

比较分析在大米培养基中添加2种樟树不同浓度叶片匀浆对牛樟芝三萜含量及抗氧化活性的影响,筛选出最适的培养条件,为牛樟芝人工培养及樟属植物资源开发利用提供参考。以大米培养基为对照,分别添加0、0.5、1、2、3、4 g共6个浓度的牛樟和香樟叶片匀浆基质,测量了6种不同处理下牛樟芝的生长特性及三萜含量,并比较其对DPPH自由基清除能力和总还原能力,以评价牛樟芝的抗氧化活性。结果表明:不同浓度的牛樟和香樟叶片匀浆基质添加均能显著提高牛樟芝的生长和生物活性,各处理分别在9~27 d完成高峰生长。经过生长特性因子综合评价得出,在大米培养基中添加3 g香樟基质显著促进了牛樟芝的生长,较对照提高了4倍,为促进牛樟芝生长的最优处理;添加1 g牛樟基质显著提高了牛樟芝的三萜含量、DPPH清除能力及总还原能力,较对照分别提高了5.2、15.8和23倍,为提高牛樟芝三萜含量及抗氧化活性的最优处理。

关键词：牛樟芝牛樟香樟三萜含量抗氧化活性

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基于单片机的倒车超声波测距系统设计

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农业装备与车辆工程 2024年第4期62卷 107-111页

作者：石小龙王智超孙婷婷盐城工学院汽车工程学院江苏盐城224051

为实现精准测距,提高驾驶安全性,对以51单片机为核心的超声波测距系统的硬件电路和软件程序进行设计,结合Proteus进行联合仿真,并依照原理图制作了产品原型。实物测试结果表明,该超声波测距系统硬件电路简单合理、性能优良、运行稳定、... 详细信息

为实现精准测距,提高驾驶安全性,对以51单片机为核心的超声波测距系统的硬件电路和软件程序进行设计,结合Proteus进行联合仿真,并依照原理图制作了产品原型。实物测试结果表明,该超声波测距系统硬件电路简单合理、性能优良、运行稳定、测距较快,可以在与障碍物距离较近时自动触发警报,在测量精度方面基本符合汽车倒车时的测距需求。

关键词：单片机测距系统超声波倒车警报

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